RESUMO
A widely applied pesticide of azoxystrobin, is increasingly detected in the water environment. Concern has been raised against its potential detriment to aquatic ecosystems. It has been shown that exposure to azoxystrobin interfere with the locomotor behavior of zebrafish larvae. This study aims to investigate whether exposure to environmental levels of azoxystrobin (2 µg/L, 20 µg/L, and 200 µg/L) changes the behavior of male adult zebrafish. Herein, we evaluated behavioral response (locomotor, anxiety-like, and exploratory behaviors), histopathology, biochemical indicators, and gene expression in male adult zebrafish upon azoxystrobin exposure. The study showed that exposure to azoxystrobin for 42 days remarkably increased the locomotor ability of male zebrafish, resulted in anxiety-like behavior, and inhibited exploratory behavior. After treatment with 200 µg/L azoxystrobin, vasodilatation, and congestion were observed in male zebrafish brains. Exposure to 200 µg/L azoxystrobin notably elevated ROS level, MDA concentration, CAT activity, and AChE activity, while inhibiting SOD activity, GPx activity, ACh concentration, and DA concentration in male zebrafish brains. Moreover, the expression levels of genes related to the antioxidant, cholinergic, and dopaminergic systems were significantly changed. This suggests that azoxystrobin may interfere with the homeostasis of neurotransmitters by causing oxidative stress in male zebrafish brains, thus affecting the behavioral response of male zebrafish.
Assuntos
Pirimidinas , Estrobilurinas , Poluentes Químicos da Água , Peixe-Zebra , Animais , Masculino , Peixe-Zebra/metabolismo , Ecossistema , Estresse Oxidativo , Colinérgicos/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/metabolismoRESUMO
Gastric cancer (GC) with pulmonary metastasis is one of the deadliest diseases in the world; however, the underlying pathological mechanisms and potential therapeutic targets remain to be elucidated. As exosomes play indispensable roles in the formation of premetastatic niches (PMN) and cancer metastasis. Therefore, investigating the underlying mechanisms of exosome-mediated pulmonary metastasis of GC may shed new light on identifying novel therapeutic targets for GC treatment. GC-derived exosomes were isolated from the conditioned medium of mouse forestomach carcinoma (MFC) cell line. The effects of MFC-derived exosomes on pulmonary macrophage polarization were analyzed by reverse- transcription polymerase chain reaction and flow cytometry. Expression of PD-L1 and other proteins was evaluated by Western blot. Exosomal microRNAs (miRNAs) were analyzed by microarray. GC-derived exosomes (GC-exo) accumulated in high numbers in the lungs and were ingested by macrophages. The extracellular-signal-regulated kinase (ERK) signaling pathway was activated by GC-exo, inducing macrophage immunosuppressive-phenotype differentiation and increased PD-L1 expression. miRNA-sequencing identified 130 enriched miRNAs in GC-exo. Among the enriched miRNAs, miR-92a-3p plays a major role in activating ERK signaling via inhibition of PTEN expression. In addition, inhibiting ERK signaling with PD98059 significantly reduced the expression of PD-L1 in macrophages and, therefore, reversed the immunosuppressive PMN and inhibited the colonization of GC cells in the lungs. This study identified a novel mechanism of GC-exo mediated PD-L1 expression in lung macrophages that facilitates lung PMN formation and GC pulmonary metastasis, which also provided a potential therapeutic target for GC with pulmonary metastasis treatment.
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Exossomos , Neoplasias Pulmonares , MicroRNAs , Neoplasias Gástricas , Animais , Camundongos , Neoplasias Gástricas/patologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Antígeno B7-H1/genética , Antígeno B7-H1/metabolismo , Exossomos/metabolismo , Linhagem Celular Tumoral , MicroRNAs/genética , MicroRNAs/metabolismo , Macrófagos/metabolismo , Neoplasias Pulmonares/metabolismoRESUMO
High-performance pesticide formulations are essential for sustainable agriculture. Among these, nano-pesticides exhibit great advantages in pest control because of their unique size effects. However, the direct effects of nano-formulation fungicides on fungal pathogens remain largely unexplored. In this study, three qualified formulations, suspension concentrate (SC), microcapsules (CS), and nanocapsules (NCS) of pyraclostrobin (PYR) were prepared and utilized to reveal their biocontrol activities against Rhizoctonia solani. Among these three formulations, NCS exhibited notable biocontrol efficacy against R. solani exemplified by an EC50 of 0.319 mg/L for mycelia, distortion of mycelia and abnormalities in cell ultrastructure. Moreover, NCS displayed excellent internalization within R. solani mycelia, contributing to severe damage to cell membrane permeability. Importantly, an equivalent quantity of NCS-PYR showed potent inhibitory effects on the target pathogen, as indicated by reduced adenosine triphosphate (ATP) content and mitochondrial Complex III activity. The NCS consistently exhibited superior in vivo protective and curative activities against R. solani compared to those of CS and SC in rice and faba bean. In summary, we uncovered the strength of rapid efficacy and biocontrol activity of NCS against R. solani and elucidated the advantages of NCS-PYR from the perspective of the target pathogen in agriculture.
Assuntos
Nanocápsulas , Doenças das Plantas/prevenção & controle , Doenças das Plantas/microbiologia , RhizoctoniaRESUMO
BACKGROUND: Nicotianamine (NA), 2'-deoxymugineic acid (DMA), and mugineic acid (MA) are chelators required for iron uptake and transport in plants. Nicotianamine aminotransferase (NAAT), 2'-deoxymugineic acid synthase (DMAS), transporter of MAs (TOM), and efflux transporter of NA (ENA) are involved in iron uptake and transport in rice (Oryza sativa), wheat (Triticum aestivum), and barley (Hordeum vulgare); however, these families have not been fully identified and comprehensively analyzed in maize (Zea mays L.). RESULTS: Here, we identified 5 ZmNAAT, 9 ZmDMAS, 11 ZmTOM, and 2 ZmENA genes by genome mining. RNA-sequencing and quantitative real-time PCR analysis revealed that these genes are expressed in various tissues and respond differently to high and low iron conditions. In particular, iron deficiency stimulated the expression of ZmDMAS1, ZmTOM1, ZmTOM3, and ZmENA1. Furthermore, we determined protein subcellular localization by transient expression of green fluorescent protein fusions in maize mesophyll protoplasts. ZmNAAT1, ZmNAAT-L4, ZmDMAS1, and ZmDMAS-L1 localized in the cytoplasm, whereas ZmTOMs and ZmENAs targeted to plasma and tonoplast membranes, endomembranes, and vesicles. CONCLUSIONS: Our results suggest that the different gene expression profiles and subcellular localizations of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA family members may enable specific regulation of phytosiderophore metabolism in different tissues and under different external conditions, shedding light on iron homeostasis in maize and providing candidate genes for breeding iron-rich maize varieties.
Assuntos
Genoma de Planta/genética , Ferro/metabolismo , Família Multigênica/genética , Proteínas de Plantas/genética , Zea mays/genética , Ácido Azetidinocarboxílico/análogos & derivados , Ácido Azetidinocarboxílico/metabolismo , Transporte Biológico , Cromossomos de Plantas/genética , Regulação da Expressão Gênica de Plantas , Genes Reporter , Homeostase , Deficiências de Ferro , Filogenia , Proteínas de Plantas/metabolismo , Transporte Proteico , Proteínas Recombinantes de Fusão , Sideróforos/metabolismo , Transaminases/genética , Transaminases/metabolismo , Zea mays/enzimologia , Zea mays/fisiologiaRESUMO
Rice crab co-culture is a new integrated farming model in China. The application of triazole plant growth regulators (PRGs) is often used as an advantageous option to combat rice lodging. However, there is still a gap regarding the toxicity of these PRGs on the growth and development of the Chinese mitten crab (Eriocheir sinensis, E. sinensis). Here the effect of triazoles (paclobutrazol and uniconazole) on the molting mechanism of E. sinensis was investigated. Monitoring of regulatory genes associated with molting showed that the two PRGs were found to inhibit the expression of ecdysteroid hormone (EH), ecdysteroid receptors gene (EcR), and retinoid X receptors gene (RXR) and induce secretion of molt-inhibiting hormone (MIH) gene. In addition, the activities of chitinase (CHIA) and N-acetyl-ß-d-aminoglucosidase (ß-NAGase) were also inhibited by exposure to PRGs. Exposure to PRGs also elevated the mRNA expression of the growth-related myostatin gene (MSTN). These results revealed that there is a long-term risk of exposure to triazoles PRGs that may inhibit molting and affect normal development and immune system of E. sinensis.
Assuntos
Braquiúros , Muda , Animais , Braquiúros/genética , Braquiúros/metabolismo , Ecdisteroides/metabolismo , Ecdisteroides/farmacologia , Muda/genética , Reguladores de Crescimento de Plantas/farmacologia , Triazóis/toxicidadeRESUMO
Unsubstituted pyridin-2-amine has a high quantum yield and is a potential scaffold for a fluorescent probe. However, the facile access to conjugated highly substituted aminopyridines and the study of their fluorescent properties is scarce. In this paper, synthesis and fluorescent properties of multisubstituted aminopyridines were studied based on a recently developed Rh-catalyzed coupling of vinyl azide with isonitrile to form a vinyl carbodiimide intermediate, following tandem cyclization with an alkyne. An aminopyridine substituted with an azide group as a potential probe was further designed, synthesized, and evaluated. The "clicking-and-probing" experiment of it on BSA protein showed the potential of aminopyridine as a scaffold of a biological probe.
RESUMO
The strobilurin fungicide azoxystrobin (AZO) can induce adverse effects in aquatic organisms, but data are lacking on endpoints associated with sexual development and reproduction following chronic exposure to AZO. In this study, zebrafish embryos (F0) at 2-4 h postfertilization (hpf) were exposed to 0.2, 2.0, and 20.0 µg/L AZO until 120 d postfertilization (dpf). Decreased male ratio and increased intersex ratio were observed by 20.0 µg/L AZO at 42 and 60 dpf, but this effect disappeared at 120 dpf. AZO at 20.0 µg/L inhibited growth, retarded gonadal development, and disrupted sex hormone and vitellogenin in females at 60 and 120 dpf and in males at 42, 60, and 120 dpf. These effects were associated with altered expression of cyp19a, cyp19b, hsd3b, hsd17b, vtg1, and vtg2. Exposure to 2.0 µg/L AZO altered mRNA levels of these transcripts in females at 120 dpf and in males at 60 and 120 dpf. Reproduction ability was reduced by 20.0 µg/L AZO at 120 dpf. Developmental defects were observed after F1 embryos from exposed parents of 20.0 µg/L were reared in AZO-free water at 96 hpf. Overall, these data provide new understanding of fish sexual development and reproduction following chronic exposures to AZO.
Assuntos
Poluentes Químicos da Água , Peixe-Zebra , Animais , Feminino , Masculino , Pirimidinas , Reprodução , Desenvolvimento Sexual , EstrobilurinasRESUMO
Broad applications and exposure to the fungicide maneb can lead to toxicity in non-target organisms. Maneb is also associated with neurogenerative diseases such as Parkinson's disease (PD). The objectives of this study were to determine the acute toxicity of maneb to zebrafish by measuring mitochondrial bioenergetics, locomotor activity, and the expression of genes related to the oxidative damage response, as well as those related to dopamine signaling due to its association with PD. Zebrafish embryos at 6â¯h post-fertilization (hpf) were exposed to either solvent control (0.1% DMSO, v/v), or one dose of 0.1, 0.5, 1.0 and 10.0⯵M maneb for 96â¯h. Maneb was moderately toxic to zebrafish embryos, and had a 96-h LC50 value of 4.29⯵M (~â¯1.14â¯mg/L). Maneb induced a dose-dependent increase in mortality, decreased hatching rate, and increased notochord deformity rate at both 1.0 and 10.0⯵M after 72 and 96â¯h. Total body length was also significantly reduced with 1.0⯵M maneb. A 50-60% decrease in mean basal oxygen consumption rate was also observed in embryos following a 24 hpf exposure to 10.0⯵M maneb but oligomycin-induced ATP production and FCCP-induced maximum respiration remained unaffected. No change was detected in the expression levels of genes associated with oxidative stress (sod1 and sod2), nor those related to dopamine synthesis (th1), dopamine transporter (dat), dopamine receptors (drd1, drd2a, drd3, and drd4b). Thus, modifying the expression of these transcripts may not be a mechanism for maneb-induced developmental toxicity in zebrafish. To assess the potential for neurotoxicity, a dark photokinesis assay was conducted in larvae following 7 d exposure to 0.1, 0.5 and 1.0⯵M maneb. Larvae exposed to 0.5 and 1.0⯵M maneb showed signs related to hypoactivity, and this reduced activity is hypothesized to be associated with notochord defects as this deformity was prevalent at higher concentrations of maneb. Overall, these data demonstrate that maneb negatively affects embryonic development (i.e. notochord development), affects basal oxygen consumption rates of embryos, and induces hypoactivity in larval fish. This study improves understanding regarding the developmental neurotoxicity of the fungicide maneb to zebrafish.
Assuntos
Embrião não Mamífero/efeitos dos fármacos , Larva/efeitos dos fármacos , Maneb/toxicidade , Mitocôndrias/efeitos dos fármacos , Notocorda/efeitos dos fármacos , Peixe-Zebra/embriologia , Animais , Proteínas da Membrana Plasmática de Transporte de Dopamina/genética , Proteínas da Membrana Plasmática de Transporte de Dopamina/metabolismo , Relação Dose-Resposta a Droga , Embrião não Mamífero/patologia , Desenvolvimento Embrionário/efeitos dos fármacos , Metabolismo Energético/efeitos dos fármacos , Feminino , Expressão Gênica , Locomoção/efeitos dos fármacos , Masculino , Mitocôndrias/patologia , Notocorda/patologia , Estresse Oxidativo/efeitos dos fármacos , Consumo de Oxigênio , Praguicidas/toxicidade , Receptores Dopaminérgicos/genética , Receptores Dopaminérgicos/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Superóxido Dismutase-1/genética , Superóxido Dismutase-1/metabolismo , Peixe-Zebra/metabolismoRESUMO
Glutathione S-transferases (GSTs) have been widely studied in relation to their role in herbicide tolerance and detoxification. However, a detailed characterization of GSTs from herbicide tolerant and sensitive maize cultivars is still lacking. In this study, we determined the mechanism of differential tolerance between two maize cultivars which had 4-fold difference tolerance to metolachlor. The metabolism rate of metolachlor was more rapid in the tolerant cultivar (Zea mays L. cv Nongda86) than the susceptible one (Zea mays L. cv Zhengda958). Addition of the GST inhibitor ethacrynic acid reduced the metabolism of metolachlor indicating the involvement of GSTs in the differential detoxification of metolachlor. The expression profiles of 32 GST isozymes were measured using quantitative RT-PCR. The results showed the expression of GST genes were slightly up-regulated in Nongda86, but severely inhibited in Zhengdan958 24h after metolachlor treatment. The genes GSTI, GSTIII, GSTIV, GST5, GST6 and GST7, which can detoxify chloroacetanilide herbicides, were all expressed higher in Nongda86 compared to Zhendgan958. The result of GST activity was consistent with the gene expression profiles. Collectively, higher-level expression of GST genes, leading to higher GST activity and faster herbicide detoxification, appears to be responsible for the difference in tolerance to metolachlor in two maize cultivars.
Assuntos
Acetamidas/toxicidade , Glutationa Transferase/genética , Resistência a Herbicidas/genética , Herbicidas/toxicidade , Zea mays/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Inativação Metabólica , Isoenzimas/genética , Proteínas de Plantas/genética , Especificidade da Espécie , Zea mays/genéticaRESUMO
Eleven new scalarane sesterterpenoids, including three 20,24-bishomo-25-norscalaranes, carteriofenones AC (13), and eight 20,24-bishomoscalaranes, carteriofenones DK (411), along with two known analogues (12 and 13), were obtained from the marine sponge Carteriospongia foliascens collected from the South China Sea. Their planar structures and relative configurations were elucidated by extensive NMR spectroscopic data. The absolute configuration of 3 was determined using the modified Mosher's method on its hydrolysis product. Scalarane sesterterpenoids with 4-methylpentanate or pentanoate substituents at the 12-position (18) were reported for the first time. Carteriofenones EH (58) represented rare naturally occurring scalarane sesterterpenoids with a cyclobutane ring. Compound 4 showed cytotoxicity against the mouse lymphocytic leukemia cell line (P388) with an IC50 value of 0.96 µM.
Assuntos
Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Poríferos/química , Sesterterpenos/isolamento & purificação , Sesterterpenos/farmacologia , Animais , Antineoplásicos/química , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Concentração Inibidora 50 , Camundongos , Sesterterpenos/químicaRESUMO
Neonicotinoid insecticides are new class of pesticides and it is very meaningful to evaluate the toxicity of guadipyr to earthworm (Eisenia fetida). In the present study, effects of guadipyr on reproduction, growth, catalase(CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE) and DNA damage in earthworm were assessed using an artificial soil medium. Guadipyr showed low toxicity to earthworms and did not elicit an effect on earthworm reproduction or growth in artificial soils at concentrations <100mg/kg. However, after exposure to guadipyr, the activity of SOD and CAT in earthworm increased and then decreased to control level. AChE activity decreased at day 3 at 50 and 100mg/kg and then increased to control level. Our data indicate that guadipyr did not induce DNA damage in earthworms at concentration of <100mg/kg.
Assuntos
Dano ao DNA , Guanidinas/toxicidade , Inseticidas/toxicidade , Oligoquetos , Poluentes do Solo/toxicidade , Acetilcolinesterase/metabolismo , Animais , Catalase/metabolismo , Ensaio Cometa , Relação Dose-Resposta a Droga , Dose Letal Mediana , Oligoquetos/efeitos dos fármacos , Oligoquetos/crescimento & desenvolvimento , Reprodução/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testes de Toxicidade Aguda , Testes de Toxicidade SubcrônicaRESUMO
A simple, low-cost, and highly sensitive method using a modified QuECHERS procedure based on a liquid chromatography-tandem mass spectrometer (LC-MS/MS) was established to simultaneously quantify lufenuron and chlorfenapyr and the corresponding metabolite tralopyril in cabbage for the first time. On the basis of this method, terminal residue and dietary risk of lufenuron and chlorfenapyr in cabbage were investigated. The recoveries of lufenuron, chlorfenapyr, and tralopyril ranged from 88 to 110%, with relative standard deviation of less than 12.4%. The field trial results showed that at the pre-harvest interval (PHI) of 21 days, the terminal residues of lufenuron, chlorfenapyr, and tralopyril in the supervised trials were not higher than 0.02 mg/kg, and the highest detected residue levels of lufenuron, chlorfenapyr, and tralopyril were 0.047, 0.055, and <0.02 mg·kg-1 at 14-day pre-harvest respectively, which were lower than the maximum residue limits (MRLs) for cabbage established in China. For the dietary risk assessment, the national estimated daily intakes (NEDIs) as proportion of acceptable daily intakes (ADIs) were 80.4% and 29.9% for chlorfenapyr and lufenuron respectively indicating an acceptable dietary risk to Chinese population.
Assuntos
Benzamidas , Brassica , Brassica/química , Medição de Risco , Piretrinas , Resíduos de Praguicidas , China , Espectrometria de Massas em Tandem , Cromatografia Líquida , Humanos , Exposição Dietética , FluorocarbonosRESUMO
Carbofuran, a widely used carbamate insecticide, is frequently detected in water. In this study, a high-performance adsorbent (WAB4) for carbofuran was obtained from laboratory-synthesized biochars. The maximum adsorption of carbofuran by WAB4 reaches 113.7 mg/g approximately. The adsorption of carbofuran by biochar was a multi-molecular layer and the adsorption process conforms to the pseudo-second-order kinetic model (R2 = 0.9984) and Freundlich isotherm model (R2 = 0.99). Importantly, an in vivo rat model was used to assess the combined toxicological effects of biochar-carbofuran complexes. The toxicity of the complexes (LD50 > 12 mg/kg) is lower than that of carbofuran (LD50 = 7.9 mg/kg) alone. The damage of biochar-carbofuran complex on rat liver and lung is significantly less than that of carbofuran. The Cmax and bioavailability of carbofuran were found to be reduced by 64% and 68%, respectively, when biochar was present, by UPLC-MS/MS analysis of carbofuran in rat plasma. Furthermore, it was confirmed that the biochar-carbofuran complex is relatively stable in the gastrointestinal tract, by performing a carbofuran release assay in artificial gastrointestinal fluids in vitro. Collectively, biochar is a bio-friendly material for the removal of carbofuran from water.
Assuntos
Carbofurano , Poluentes Químicos da Água , Animais , Ratos , Carbofurano/toxicidade , Adsorção , Água , Cromatografia Líquida , Espectrometria de Massas em Tandem , Carvão Vegetal , Cinética , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/análise , Concentração de Íons de HidrogênioRESUMO
A new indole-diterpenoid, penijanthine E (1), and a known analogue (2), were obtained from the PDB culture of the marine-derived fungus Penicillium citrinum ZSS-9. The absolute configuration of 1 was elucidated by calculated TDDFT ECD and DP4plus calculations. The absolute configuration of 2 was confirmed by single-crystal X-ray diffraction analysis and TDDFT ECD calculations. Compounds 1 and 2 showed antiviral activity against influenza A virus (IAV) of A/WSN/33(H1N1) and A/PR/8/34(H1N1) strains with IC50 values ranging from 12.6 to 46.8 µM.
Assuntos
Diterpenos , Vírus da Influenza A Subtipo H1N1 , Penicillium , Estrutura Molecular , Penicillium/química , Diterpenos/química , Indóis/farmacologia , Indóis/química , FungosRESUMO
Introduction: While the integrated rice-crayfish (Procambarus clarkii) farming system (IRCFS) is widely developing in China, the widespread use of Unmanned Aerial Spraying Systems (UASS) to protect rice from pests has led to potential pesticide risk for the crayfish in IRCFS. Therefore, it is crucial to examine UASS's spray deposition and drift in IRCFS. Method: In this study, we used the oligonucleotide sequence-tracking / dot-blotting (OSTDB) method to trace pesticide spraying. We collected detailed data not only on spray loss in the paddy fields, but also on spray drift in the breeding ditches caused by upwind and downwind spray areas. Additionally, pesticide residues in the breeding ditches were measured using LC-MS/MS by collecting water samples after pesticide application. Results: The data analysis indicated that the spray loss in the paddy field was significantly greater than that in the breeding ditches. The spray drift in the breeding ditches, caused by the upwind spray area, was seven times higher than that originating from the downwind spray area. Furthermore, the results also revealed that the bulk flow between the paddy fields and the breeding ditches contributed a substantial amount of pesticide residue to the water body in the breeding ditches. In addition, we investigated the acute toxicities of common insecticides using in paddy fields, including thiamethoxam (THI), chlorantraniliprole (CHI), THI·CHI-Mix and THI·CHI-WG. Discussion: The results demonstrated that the spray losses and spray drift from UASS spray applications of these pesticides in IRCFS would not cause acute toxicity or death in crayfish. These findings provide important materials for establishing pesticide application standards and guiding the field testing of droplet deposition and drift in IRCFS.
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The strip filling method in underground reservoir needs high strength to achieve the requirements of water storage. In order to address the challenges associated with costly and weak filling materials, this study aimed to develop an economically efficient and high-strength gangue-based geopolymer backfill material (GBGBM). To achieve this, the Taguchi method was employed to design a series of 25 experiments, each consisting of four factors and five levels. This study focused on investigating the effects of different gangue gradation levels, sand ratios, water binder ratios (w/b), and aggregate binder ratios (a/b) on the working characteristics and unconfined compressive strength (UCS) of the GBGBM. The optimal combination of the GBGBM was determined by employing a signal-to-noise ratio (S/N)-based extreme difference and variance analysis. The results revealed that the w/b ratio exerted the most substantial influence on both the slump and UCS. Specifically, when employing a gradation of 50%, a sand ratio of 55%, an a/b ratio of 2.5, and a w/b ratio of 0.64, the slump measured 251.2 mm, the UCS at 3d reached 5.27 MPa, and the UCS at 28d amounted to 17.65 MPa. These findings indicated a remarkable improvement in early UCS by 131.14% and the late UCS by 49.45% compared to gangue-based cement backfill material (GBCBM). Additionally, this study examined the hydration products and microstructures of both GBGBM and GBCBM using X-ray diffraction (XRD), scanning electron microscopy (SEM), and mercury intrusion porosimetry (MIP) analyses. Significantly, the GBGBM exhibited notable advantages over the GBCBM, including a 78.16% reduction in CO2 emissions, a 73.45% decrease in energy consumption, and a 24.82% reduction in cost. These findings underscore the potential of GBGBM as a sustainable and cost-effective alternative to GBCBM.
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Tricyclazole is used as a common fungicide to control rice blast. However, studies on the toxicity of tricyclazole to crabs in the rice-crab co-culture system are still extremely rare. Here, the environmental dissipation of tricyclazole was monitored in this model, and the potential toxicity of tricyclazole to E. sinensis at environmental concentrations as well as the dietary risk was evaluated. The results showed that tricyclazole had no significant acute toxicity to E. sinensis (LC50 > 100 mg/L), while it promoted body weight gain. Tricyclazole in the hepatopancreas had a higher persistent bioaccumulation risk than in the muscle. Tricyclazole suppressed the immune response of E. sinensis under prolonged exposure and there should be gender differences, with females being more sensitive. Lipid metabolism enzymes were also significantly inhibited. While tricyclazole stimulated males molting but prolonged molting duration, both molting and duration of females were also disturbed. The dietary risk assessment indicated that tricyclazole intake from current crab consumption was low risk. This evidence demonstrated that tricyclazole may have potential risks to individual development, nutritional quality, and economic value on E. sinensis and should be used with caution in rice-crab co-culture system whenever possible.
Assuntos
Braquiúros , Hepatopâncreas , Animais , Feminino , Masculino , Técnicas de Cocultura , Hepatopâncreas/metabolismo , Alimentos Marinhos , ChinaRESUMO
Given the inevitable exposure of Eriocheir sinensis (E. sinensis) to fungicides in rice-crab co-culture systems, understanding the potential effect of fungisides is important for practical application. Molting is a crucial development process of E. sinensis, which is regulated by endocrine system and genetic factors, and is susceptible to exogenous chemicals. However, the impact of fungicides application on the molting of E. sinensis have been rarely reported. In the present study, propiconazole, a widely used fungicide for rice disease management, was found to exert potential effects on the molting of E. sinensis at residual-related level in the rice-crab co-culture fields. After 14 days of short-term exposure to propiconazole, female crabs exhibited remarkably higher levels of hemolymph ecdysone than males. When the exposure was extended to 28 days, propiconazole markedly accelerated molt-inhibiting hormone expression by 3.3-fold, ecdysone receptor expression by 7.8-fold, and crustacean retinoid X receptor expression by 9.6-fold in male crabs, while it showed the opposite effect in females with suppressed gene expression. Propiconazole also induced the activity of N-acetylglucosaminidase in male crabs rather than females during the experiments. Our study suggests that propiconazole exerts sex-specific effects on the molting of E. sinensis. The impact of propiconazole application in the rice-crab co-culture systems remains more assessment to avoid affecting the growth of cultured E. sinensis.
Assuntos
Braquiúros , Fungicidas Industriais , Animais , Masculino , Feminino , Muda/genética , Fungicidas Industriais/toxicidade , Triazóis/toxicidadeRESUMO
Azoxystrobin, a strobilurin widely used to control rice diseases, has raised concerns about possible adverse effects on aquatic ecosystems. At present, very little is known about the effects of azoxystrobin on courtship and aggressive behavior and the potential underlying mechanisms. In the present study, after exposing adult male and female zebrafish to worst-case scenario concentrations of azoxystrobin (0, 2 µg/L, 20 µg/L, and 200 µg/L) for 42 d, we observed a decrease in courtship behavior and an increase in aggressive behavior in both male and female zebrafish. In addition, to elucidate the molecular mechanism of the behavioral effects of azoxystrobin, we quantified the changes in the concentrations of kisspeptin, 5-HT, GnIH, and their corresponding receptor mRNA expression in the brain. The results showed that 200 µg/L azoxystrobin decreased the concentrations of kisspeptin and increased the concentration of GnIH in both male and female zebrafish brain. In addition, azoxystrobin also significantly reduced 5-HT concentration in female zebrafish brain. Further investigation revealed that altered courtship and aggressive behavior were associated with the expression levels of genes (kiss1, kiss2, gnrh3, gnrhr3, 5ht1a, and 5ht2a) involved in kisspeptin-GnIH signaling pathway. In conclusion, our study suggested that azoxystrobin may impair courtship and aggressive behavior in zebrafish by interfering with the kisspeptin-GnIH signaling pathway, which may have more profound effects on natural zebrafish populations.
Assuntos
Kisspeptinas , Peixe-Zebra , Animais , Feminino , Masculino , Peixe-Zebra/metabolismo , Estrobilurinas/toxicidade , Estrobilurinas/metabolismo , Kisspeptinas/genética , Kisspeptinas/metabolismo , Corte , Ecossistema , Serotonina , Transdução de SinaisRESUMO
Tricyclazole, propiconazole, imidacloprid, and thiamethoxam are commonly used pesticides in paddy fields. It is necessary and practical to remove pesticides from the water environment because the low utilization rate of pesticides will produce residues in the water environment. It is known that there are few studies on the preparation of biochar adsorption pesticides by the walnut shell and few studies on the removal of tricyclazole and propiconazole. Based on this, this paper used the walnut shell as raw material and boric acid as an activator to prepare biochar by the one-step method. The boric acid modified walnut shell biochar (WAB4) with a specific surface area of 640.6 m2 g-1, exhibited the high adsorption capacity of all four pesticides (>70%) at pH 3-9. The adsorption capacities of tricyclazole, propiconazole, imidacloprid, and thiamethoxam were 171.67, 112.27, 156.40, and 137.46 mg g-1, respectively. The adsorption kinetics fitted the pseudo-second-order kinetic model and the adsorption isotherm curves conformed to the Freundlich isotherm model. The adsorption of pesticides by WAB4 was associated with hydrogen bonding, pore filling, hydrophobic effects, and π-π interactions. More significantly, WAB4 has excellent adsorption capacity compared to other adsorbents for real water samples. Finally, walnut shell biochar has no significant acute toxicity to Daphnia magna. This work shows that walnut shell-based biochar has a good effect on the removal of pesticides at a wide range of pH and is economical and safe, providing a new idea for the removal of pesticides in water.