Nitrogen metabolism in lambs fed diets containing peach palm meal.

The objective of this study was to evaluate the nitrogen metabolism of lambs fed diets containing peach palm meal substituting maize (0, 10, 40, 60, and 85 % DM). Thirty Santa Inês uncastrated lambs with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. The nitrogen (N) intake (g/day, %BW) and the fecal N decreased linearly as the peach palm meal was added to the concentrate. The urinary N, however, responded quadratically, with maxima at the level of 29.97 and 40.44 % of substitution of maize for peach palm meal when expressed in g/day and %BW, respectively. The substitution of maize for peach palm meal reduced the retention of nitrogen and the microbial protein synthesis in the lambs.

Intake, digestibility and performance of lambs fed diets containing peach palm meal.

The aim of this study was to evaluate the intake and apparent digestibility of nutrients, performance, and plasma glucose concentration of ram lambs fed diets containing peach palm meal substituting maize (0, 10, 40, 60, and 85 % dry matter (DM)). Thirty Santa Inês rams with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. The substitution of the maize for the peach palm meal affected (P < 0.05) the intakes of DM, organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), total carbohydrates (TC), total digestible nutrients (TDN), and metabolizable energy (ME), which decreased linearly (P < 0.05); the intake of ether extract (EE), however, fit an increasing linear equation (P < 0.05). The apparent digestibility coefficients of DM, OM, NDFap, and TC decreased linearly (P < 0.05) as the level of peach palm meal in the concentrate was increased. The total weight gain and the average daily gain decreased by 0.09 and 0.001 kg with each level of substitution of the maize for peach palm meal, respectively. It is recommended to substitute 40 % of the maize for peach palm meal.

Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal.

The objective was to evaluate the influence of diets on lambs using different levels of peach palm meal as a replacement for maize (0, 10, 40, 60, and 85% of diet dry matter) on the endogenous creatinine clearance (CC), urine concentration ratio of purine derivatives to creatinine (PDC index), and daily creatinine excretion (DCE) as a marker to estimate purine derivatives (PD) excretion from urinary spot samples collected at different time points (4, 8, 12, 16, 20, 24 h after morning feeding) compared to 24-h total urine collection. The measured parameters were voluntary intake, urinary volume, CC, DCE, the concentration of plasma creatinine, and PD and purine derivatives' excretion (PDE). Five lambs were allocated to metabolic cages and distributed in a 5 × 5 Latin square. Urine collection was taken daily on days 16 to 19 of each experimental period. The inclusion of peach palm meal linearly reduced the intake of dry matter (g kg BW−0.75, p = 0.005), crude protein (g kg BW−0.75, p = 0.010), metabolizable energy (MJ kg BW−0.75, p = 0.010) and CC (p < 0.0001). It also quadratically affected the urinary volume (p = 0.008) and DCE (p = 0.004). There was a linear decrease for PDC index (p = 0.032) and PDE (p < 0.0001) measured in the 24-h total urine with peach palm meal levels. The different times of spot urine sampling did not affect (p > 0.05) the PDC index and PDE. Peach palm meal decreases the CC thereby compromising the use of a mean value of DCE as a PDE marker in spot urine samples. There is greater accuracy when using different values of DCE obtained for each diet as markers for the PDE in spot urine samples. Unconventional foodstuffs of low palatability affecting the voluntary intake of feed change the renal function.

Metabolizable Protein: 1. Predicting Equations to Estimate Microbial Crude Protein Synthesis in Small Ruminants.

Microbial crude protein (MCP) produced in rumen could be estimated by a variety of protocols of experimental sampling and analysis. However, a model to estimate this value is necessary when protein requirements are calculated for small ruminants. This model could be useful to calculate rumen degradable protein (RDP) requirements from metabolizable protein (MP). Then, our objective was to investigate if there is a difference in MCP efficiency between sheep and goats, and to fit equations to predict ruminal MCP production from dietary energy intake. The database consisted of 19 studies with goats (n = 176) and sheep (n = 316), and the variables MCP synthesis (g/day), total digestible nutrients (TDN), and organic matter (OM) intakes (g/day), and OM digestibility (g/kg DM) were registered for both species. The database was used for two different purposes, where 70% of the values were sorted to fit equations, and 30% for validation. A meta-analytical procedure was carried out using the MIXED procedure of SAS, specie was considered as the fixed dummy effect, and the intercept and slope nested in the study were considered random effects. No effect of specie was observed for the estimation of MCP from TDN, digestible Organic Matter (dOM), or metabolizable energy (ME) intakes (P > 0.05), considering an equation with or without an intercept. Therefore, single models including both species at the same fitting were validated. The following equations MCP (g/day) = 12.7311 + 59.2956 × TDN intake (AIC = 3,004.6); MCP (g/day) = 15.7764 + 62.2612 × dOM intake (AIC = 2,755.1); and MCP (g/day) = 12.7311 + 15.3000 × ME intake (AIC = 3,007.3) presented lower values for the mean square error of prediction (MSEP) and its decomposition, and similar values for the concordance correlation coefficient (CCC) and for the residual mean square error (RMSE) when compared with equations fitted without an intercept. The intercept and slope pooled test was significant for equations without an intercept (P < 0.05), indicating that observed and predicted data differed. In contrast, predicted and observed data for complete equations were similar (P > 0.05).

Effects of alkaloid extracts of mesquite pod on the products of in vitro rumen fermentation.

The objective of this study was to evaluate the effects of alkaloid extracts of Prosopis juliflora (Sw.) D.C. pods obtained by two extraction methods as compared with sodium monensin on the gas production kinetic, mitigation of methane, and rumen fermentation products using wheat bran or Tifton 85 hay as substrates, by the semi-automatic in vitro gas production technique. A completely randomized design was adopted, and two natural additives were tested made from mesquite pod (alkaloid extract I and alkaloid extract II) at three levels (3.9, 7.9, and 12 µg), sodium monensin 5 µM (positive control), and no inclusion of additives (negative control). The volume of gases produced by the degradation of the fibrous fraction of wheat bran was influenced by the concentration of the extract I added to the medium, and the amounts of 7.9 and 12 µg were equal to monensin at the lowest value. The degradation rate of the fibrous carbohydrates with additive extract I at 12 µg was lower in relation to monensin. When Tifton 85 hay was utilized, alkaloid extract I provided a shorter colonization time as compared with monensin at the added amounts of 7.9 and 12 µg and higher production of gases from the fibrous fraction but without interfering with the total volume of gases produced during 96 h of fermentation of carbohydrates. In the periods of 12 and 24 h of incubation, utilizing alkaloid extract I, the mean values of methane production with wheat bran and Tifton 85 hay were lower than monensin (p < 0.05) when the respective amounts of 7.9 and 12 µg were added. Alkaloid extract I has similar potential to sodium in reducing production of total gases, methane, and the acetate/propionate ratio.

Atividade antioxidante e correlação com fenólicos totais em genótipos de Urucum (Bixa orellana L) / Antioxidant activity and correlation with total phenolicin genotypes of Annatto (Bixa orellana L)

O estudo objetivou quantificar os fenóis totais em cinco genótipos de Bixa orellana (Piave vermelha, Piavevermelha gigante, CPATU 0060, Bico-de-pato I e Peruana Paulista). Os extratos foram obtidos em etanol PAe solução hidroetanólica (80:20,v.v-1), sendo os resultados obtidos comparados a dois antioxidantes sintéticos, butil-hidroxitolueno (BHT) e ácido gálico (GAE). Os teores de fenólicos totais dos genótipos analisados apresentaram valores médios de 776,02 a 1.498,48 mg GAE.100 g-1 de amostra (peso seco) e 297,08 a 450,97 mgGAE.100 g-1, para os extratos hidroetanólicos e etanólicos, respectivamente. A medida da atividade antioxidante para os extratos hidroetanólicos variou entre 48,31 e 70,10. Valores inferiores foram obtidos para atividade antioxidante dos extratos etanólicos (18,91 a 35,26). Os resultados evidenciaram que o genótipo CPATU0060 apresentou teores de fenólicos totais e atividade antioxidante superiores aos demais genótipos analisados. Foi observada baixa correlação entre as variáveis de fenóis totais e atividade antioxidante para os extratos hidroetanólicos (R= -0,5328) e etanólicos (R= 0,4115). Considerando a ampla aplicação do urucum na indústria de alimentos e farmacêutica, os resultados sugerem que seus grãos podem ser considerados uma fonte potencial de antioxidante natural.