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1.
Pharm Biol ; 50(4): 523-8, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22136205

RESUMO

CONTEXT: Ursolic acid is a pentacyclic triterpenoid which has hepatoprotective and antihepatotoxic activities. OBJECTIVE: This study investigated whether ursolic acid is able to stimulate liver regeneration in partially hepatectomized mice. MATERIALS AND METHODS: Ursolic acid or the vehicle solution was orally administered to the experimental, sham-operated and vehicle-treated group mice for 7 days, positive control animal (mice) was treated with recombinant human hepatocyte growth factor (rhHGF), and then the 70% liver partial hepatectomy was performed. The liver mass recovery rate was estimated by measuring the ratios of mice liver weight to body weight. The liver cells undergoing DNA synthesis were identified by immunohistochemistry analysis using monoclonal anti-BrdU antibodies. The expression levels of cyclin D1, cyclin E and C/EBP proteins (C/EBPα and C/EBPß) were detected by the Western blotting technique. RESULTS: Our results showed administration of ursolic acid significantly increased the ratio of the liver to body weight and BrdU labeling index at 36 and 48 h after partial hepatectomy, and the potency of UA is similar to rhHGF treated positive control mice. In addition, ursolic acid treatment significantly increased cyclin D1, cyclin E and C/EBPß protein expression levels at 36 h after liver PHx compared with the vehicle-treated control mice. DISCUSSION AND CONCLUSION: All these results suggest that ursolic acid stimulates liver proliferation after partial hepatectomy, and this effect may be associated with the stimulation of C/EBPß expression.


Assuntos
Hepatectomia , Regeneração Hepática/efeitos dos fármacos , Fígado/efeitos dos fármacos , Triterpenos/farmacologia , Administração Oral , Animais , Western Blotting , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Proteína beta Intensificadora de Ligação a CCAAT/metabolismo , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ciclina D1/metabolismo , Ciclina E/metabolismo , Replicação do DNA/efeitos dos fármacos , Fator de Crescimento de Hepatócito/farmacologia , Humanos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Fatores de Tempo , Triterpenos/administração & dosagem , Ácido Ursólico
2.
World J Gastroenterol ; 13(31): 4264-7, 2007 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-17696259

RESUMO

AIM: To evaluate the efficacy and safety of entecavir (ETV) in hepatitis Be antigen (HBeAg)-positive chronic hepatitis B (CHB) patients who had not received a nucleoside analogue and who had failed in lamivudine (LVD) therapy. METHODS: Sixty-one patients were divided into three groups. Forty-two patients who had not received a nucleoside analogue were randomized into two groups: group A (n = 21) received LVD 100 mg/d and group B (n = 21) received ETV 0.5 mg/d. The remaing 19 patients treated with LVD (n = 19), who switched to ETV 1.0 mg/d served as group C. All patients were treated for 48 wk. HBV DNA levels were measured with polimerase-chain-reaction (PCR) analysis. Liver function tests, HBV serology and safety assessments were also conducted. RESULTS: Significantly more patients in group B (52.1% and 71.4%) had undetectable HBV DNA levels than in groups A (35.8% and 38%; P < 0.0001) and C (10.6% and 21.1%, P < 0.0001) at wk 24 and 48, respectively. At wk 48, ALT levels were normalized in more patients in group B (85.7%) than in groups A (76.2%) and C (74%). CONCLUSION: ETV had a significantly higher response rate than LVD in patients with HBeAg-positive CHB who had not previously received a nucleoside analogue; ETV can effectively inhibit the replication of HBV DNA and normalize the levels of ALT in refractory CHB patients treated with LVD; and ETV is safe in clinical application.


Assuntos
Antivirais/uso terapêutico , Guanina/análogos & derivados , Antígenos E da Hepatite B/sangue , Hepatite B Crônica/tratamento farmacológico , Hepatite B Crônica/imunologia , Adulto , Idoso , Antivirais/efeitos adversos , DNA Viral , Progressão da Doença , Relação Dose-Resposta a Droga , Feminino , Guanina/efeitos adversos , Guanina/uso terapêutico , Vírus da Hepatite B/genética , Vírus da Hepatite B/fisiologia , Humanos , Lamivudina/uso terapêutico , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Replicação Viral/efeitos dos fármacos
3.
World J Gastroenterol ; 13(41): 5440-5, 2007 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-17907286

RESUMO

AIM: To investigate the anti-viral mechanism of combination therapy of interferon (IFN)-alpha and ribavirin in patients with chronic hepatitis B. METHODS: Twenty patients were assigned to receive either IFN-alpha plus ribavirin (group A, n = 14) or no treatment as a control (group B, n = 6). Patients were analyzed for T-cell proliferative responses specific for hepatitis B virus (HBV)-antigen and cytokine production by peripheral blood mononuclear cells (PBMCs). RESULTS: Combination therapy induced HBV-antigen specific CD4+ T-cell proliferative responses in four patients (28.6%). Production of high levels of HBV-specific IFN-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-12 by PBMCs was found in five patients (35.7%), who showed significantly lower HBV DNA levels in serum at 12 mo after treatment ended (P = 0.038) and at 24 mo of follow-up (P = 0.004) than those without high levels of cytokine production. CONCLUSION: HBV-antigen specific CD4+ T cells may directly control HBV replication and secretion of anti-viral T helper 1 (Th1) cytokines by PBMCs during combination therapy of chronic hepatitis B with ribavirin and IFN-alpha.


Assuntos
Antivirais/uso terapêutico , Linfócitos T CD4-Positivos/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Citocinas/sangue , Hepatite B Crônica/tratamento farmacológico , Interferon-alfa/uso terapêutico , Ativação Linfocitária/efeitos dos fármacos , Ribavirina/uso terapêutico , Células Th1/efeitos dos fármacos , Adulto , Idoso , Antivirais/farmacologia , Linfócitos T CD4-Positivos/imunologia , DNA Viral/sangue , Quimioterapia Combinada , Feminino , Antígenos da Hepatite B/imunologia , Vírus da Hepatite B/efeitos dos fármacos , Vírus da Hepatite B/genética , Vírus da Hepatite B/imunologia , Hepatite B Crônica/genética , Hepatite B Crônica/imunologia , Hepatite B Crônica/metabolismo , Humanos , Interferon alfa-2 , Interferon-alfa/farmacologia , Interferon gama/sangue , Interleucina-12/sangue , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Proteínas Recombinantes , Ribavirina/farmacologia , Células Th1/metabolismo , Fatores de Tempo , Resultado do Tratamento , Fator de Necrose Tumoral alfa/sangue , Replicação Viral/efeitos dos fármacos
4.
World J Gastroenterol ; 12(29): 4656-9, 2006 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-16937435

RESUMO

AIM: To evaluate the effectiveness of ultrasonographic screening for early detection of hepatocellular carcinoma (HCC). METHODS: The data of 14968 patients who had ultrasonography (US) for chronic liver diseases were collected into a database program from June 1995 to June 2005. The risk factors for HCC were also studied. A total of 6089 patients who had repeated US were enrolled, 264 patients were diagnosed with HCC during follow-up (mean, 39 mo). RESULTS: The detection rate of small HCC (<= 3 cm in diameter) was 67.7%. The tumor size detected by screening at the intervals of 6 mo was significantly smaller than that at longer intervals. Only 29.3% of HCC patients had an elevated serum alpha fetoprotein (AFP) level above 400 ng/mL. The risk of HCC development during follow-up was higher in patients with liver cirrhosis (10.9%) and hepatitis C (9.0%) than in patients with chronic hepatitis (4.2%), hepatitis B (4.9%) and non-B, non-C hepatitis (NBNC, 3.9%). CONCLUSION: US screening at a interval of 6 mo is beneficial to high-risk patients over 40 years old and the early detection of HCC prolongs survival.


Assuntos
Carcinoma Hepatocelular/sangue , Carcinoma Hepatocelular/diagnóstico por imagem , Neoplasias Hepáticas/sangue , Neoplasias Hepáticas/diagnóstico por imagem , alfa-Fetoproteínas/análise , Adulto , Fatores Etários , Biomarcadores Tumorais/sangue , Carcinoma Hepatocelular/etiologia , Doença Crônica , Humanos , Hepatopatias/complicações , Neoplasias Hepáticas/etiologia , Programas de Rastreamento/métodos , Prognóstico , Fatores de Risco , Análise de Sobrevida , Ultrassonografia
5.
World J Gastroenterol ; 12(26): 4273-5, 2006 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-16830394

RESUMO

Percutaneous liver biopsy is considered one of the most important diagnostic tools to evaluate diffuse liver diseases. Pseudoaneurysm of hepatic artery is an unusual complication after ultrasound-guided percutaneous liver biopsy. Delayed hemorrhage occurs much less frequently. We report a case of pseudoaneurysm of the hepatic artery of a 46-year-old man who was admitted for abdominal pain after 4 d of liver biopsy. The bleeding was controlled initially by angiographic embolization. However, recurrent bleeding could not be controlled by repeat angiography, and the patient died 4 d after admission from multiorgan failure. The admittedly rare possibility of delayed hemorrhage should be considered whenever a liver biopsy is performed.


Assuntos
Falso Aneurisma/etiologia , Biópsia por Agulha/efeitos adversos , Hemorragia/etiologia , Artéria Hepática/lesões , Fígado/patologia , Falso Aneurisma/diagnóstico , Biópsia por Agulha/métodos , Embolização Terapêutica , Endossonografia/métodos , Evolução Fatal , Hemorragia/diagnóstico , Hemorragia/terapia , Humanos , Fígado/irrigação sanguínea , Fígado/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Recidiva , Fatores de Tempo
6.
World J Gastroenterol ; 11(1): 122-6, 2005 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-15609410

RESUMO

AIM: To investigate the effect of Boschniakia rossica (BR), oxymatrine (OM) and interferon-alpha (IFN-alpha) 1b on the therapy of rat liver fibrosis and its mechanism. METHODS: By establishing a rat model of pig serum-induced liver fibrosis, liver/weight index and serum alanine transaminase (ALT) were observed to investigate the therapeutic effect of BR,OM and IFN-alpha. Radioimmunoassay was utilized to measure procollagen type III (PCIII) and collagen type IV (CIV). RT-PCR was used to assay the expression of liver transforming growth factor-beta 1 (TGF-beta1) mRNA. Immunohistochemistry of alpha-smooth muscle actin (alpha-SMA) and pathologic changes of liver tissues were also under investigation. RESULTS: Serum PCIII and CIV in BR, OM and IFN-alpha groups were significantly declined compared with those in model group, and their RT-PCR revealed that TGF-beta1 mRNA expression was also reduced more than that in model group. Immunohistochemistry demonstrated that alpha-SMA also declined more than that in model group. Serum ALT in IFN-alpha, control and model groups was within normal level. Serum ALT in BR group had no significant difference from those of IFN-alpha, control and model groups. Serum ALT in OM group was significantly higher than those in BR, IFN-alpha, model, and control groups. CONCLUSION: BR, OM and IFN-alpha can prevent pig serum-induced liver rat fibrosis by inhibiting the activation of hepatic stellate cells and synthesizing collagen. OM has hepatotoxicity to rat liver fibrosis induced by pig serum.


Assuntos
Alcaloides/farmacologia , Antivirais/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Interferon-alfa/farmacologia , Cirrose Hepática/tratamento farmacológico , Orobanchaceae , Animais , Biomarcadores , Proteínas Sanguíneas , Modelos Animais de Doenças , Imuno-Histoquímica , Cirrose Hepática/patologia , Masculino , Tamanho do Órgão , Quinolizinas , RNA Mensageiro/análise , Ratos , Ratos Wistar , Suínos , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta1
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