Levels of leucaena hay associated with cactus pear in diets for crossbred steers.

Our hypothesis is that the total replacement of ground corn grain (GCG) by cactus pear (CP) in association with 70% of leucaena hay (LH) increases dry matter (DM) intake and digestibility and nitrogen metabolism and maintains the feeding behavior of cattle. The objective was to evaluate the effects of inclusion of these feeds on the nutritional parameters and nitrogen metabolism of cattle. Eight steers, average age of 12 months, weight of 267.5 ± 15 kg, were distributed into two simultaneous 4 × 4 Latin squares according to a 2 × 2 factorial scheme, in which the first factor evaluated was different proportions of inclusion of LH (50 or 70% DM basis), and the second factor was the total replacement of GCG with CP. There was lower DM intake in animals fed with 70% LH. There was an interaction between the factors for DM digestibility, in which the animals fed CP did not differ regardless of the inclusion of LH, but for animals without CP, the greater inclusion of LH decreases DM digestibility. The inclusion of CP decreased free water intake. There was an interaction for total water intake (WI), in which the animals fed without CP presented similarity for this, while in the inclusion of CP, the animals fed 70% of LH presented higher WI. Also, lower values of chewing were observed for animals fed with CP. The replacement of GCG with CP improves the efficiency of nitrogen utilization in the rumen. The inclusion of 70% LH depresses intake.

Inclusion of calcium oxide in sugarcane silage under different particle sizes on performance of dairy cows.

Our objective was to evaluate voluntary intake, apparent digestibility, performance, and nitrogen balance of dairy cows fed sugarcane silage under different particle sizes treated or not with calcium oxide (CaO). Eight F1 Holstein/Zebu cows, with body weight of 521.5 ± 55.17 kg, and 60 ± 10 days in milk were used, distributed into two simultaneous 4 × 4 Latin squares. The treatments were composed of sugarcane into two particle sizes (15 and 30 mm) with and without the addition of CaO (10 g/kg of natural matter), being the treatments compared according to a 2 × 2 factorial arrangement. Data were analyzed by the MIXED procedure of SAS. The intake of dry matter (13.05 kg/day), crude protein, non-fibrous carbohydrates, and neutral detergent fiber was not modified (P > 0.05) by the inclusion of the calcium oxide, nor by particles size and interaction among both. However, there was interaction between CaO and particle size on dry matter digestibility (P = 0.002), being that the CaO was effective in promoting greater DM digestibility in silages with larger particle size. The milk yield and composition were also not affected by the diets (P > 0.05), similarly for nitrogen balance (P > 0.05). The addition of CaO to sugarcane silage with different particle sizes (15 and 30 mm) does not affect the milk yield and composition of dairy cows, nor the nitrogen balance. However, when CaO is added to the sugarcane silage under larger particle sizes, there are benefits in DM digestibility.

Intake, digestibility, milk yield and composition, and ingestive behavior of cows supplemented with byproducts from biodiesel industry.

This trial aimed to analyze the effects of including three byproducts from the biodiesel industry on the intake, digestibility, milk yield and composition, and feeding behavior of lactating cows. Eight crossbred Holstein-Zebu lactating cows with average body weight 525 ± 18.5 kg and average milk yield of 8 ± 1.45 kg day-1 were assigned to four treatments (diets) in a double-Latin square design, as follows: a diet based on corn- and soybean meal-based concentrate and three diets with 20% inclusion of byproducts from the biodiesel industry (cottonseed cake, sunflower meal, and castor bean cake) on a total dry matter basis. The cows were housed in individual covered stalls with concrete floor equipped with individual concrete troughs for feeding and automatic drinkers, and fed diets containing 60% sugarcane and 40% concentrate. The inclusion of the byproducts in the diet changed the intake, digestibility of some nutritional components, milk yield and composition, and feeding behavior of lactating cows. The use of cottonseed cake and sunflower meal in the diet increased milk yield, and fat-corrected milk yield; while the use of castor bean cake reduced the intake, digestibility of dry matter and total digestible nutrients, milk yield, and fat-corrected milk yield. The inclusion of byproducts from the biodiesel industry in the diets did not change the fat, lactose, total solids, and solids-not-fat of milk. Therefore, the cottonseed cake and sunflower meal can be included at up to 20% of the total diet.

WITHDRAWN:Performance and meat quality of lambs fed detoxified castor meal.

Ahead of Print article withdrawn by publisher.

Feeding behavior of feedlot lambs fed diets containing levels of cassava wastewater.

In this study, we evaluated the effects of including cassava wastewater in the diet on the feeding behavior of feedlot lambs in 35 male uncastrated Santa Inês × Dorper crossbred lambs at an approximate age of 3 months, with an average live weight of 20.0 ± 3.4 kg. Diets were formulated with hay of cassava shoots (roughage) and a concentrate based on corn and soybean, with a roughage:concentrate ratio of 50:50, plus inclusion of cassava wastewater at the levels of 0, 12, 24, 36, or 48 g/kg of the total diet. Feeding behavior was evaluated between the 46th and 52nd days of the experiment. Increasing cassava wastewater levels in the diet reduced (P < 0.05) the intakes (kg/day) of dry matter and neutral detergent fiber as well as the efficiency of rumination (g/cud and g/h) of dry matter and neutral detergent fiber. The other behavioral parameters were not affected by wastewater inclusion in the diet. Therefore, the inclusion of up to 48 g/kg of cassava wastewater on fresh matter of diets is not recommended for feedlot lambs.

Ingestive behavior and nitrogen balance of confined santa ines lambs fed diets containing soybean hulls.

The objective of this study was to assess the effect of substituting corn with soybean hulls on the ingestive behavior and nitrogen balance of Santa Ines lambs. A total of 25 lambs with an initial body weight of 20±2 kg at approximately six months of age, sheltered individually in stalls (1.10 m×1.0 m), considering an entirely casual experimental delineation. Soybean hulls were substituted for corn at 0, 250, 500, 750, and 1,000 g/kg of dry matter (DM). The time spent feeding, ruminating, masticating, and resting was not affected by the substitution of corn with soybean hulls. In fact, the feeding efficiency in g DM/h and the rumination efficiency in g DM/bolus increased linearly with soybean hull substitution in the feed. Although the nitrogen balance was not altered by the use of soybean hulls as a substitute for corn in the diets of Santa Ines lambs, the N ingested and N digested expressed in g/d, N retained as a percentage of that ingested, and N retained as a percentage of that digested displayed quadratic behavior. In conclusion, corn can be substituted with soybean hulls up to 1,000 g/kg of dry matter in the concentrate, without changing the ingestive behavior and nitrogen balance.

Long-term Bias of Internal Markers in Sheep and Goat Digestion Trials.

Two digestion trials, one with sheep and another with goats, were conducted to evaluate the long-term bias (LTB) of the indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) internal markers. The study used eight Santa Inês castrated male sheep (average body weight of 16.6 kg) distributed in two 4×4 Latin squares and eight Saanen castrated male goats (average body weight of 22.6 kg) distributed in two 4×4 Latin squares. The experiments were conducted simultaneously, and the animals were housed in 1.2 m(2) individual pens with wood-battened floors equipped with individual feeders and drinkers. The animals received isonitrogenous diets that were offered ad libitum and contained 14% crude protein and 70% sugar cane (with 0, 0.75, 1.5 or 2.25% CaO, in natural matter percentage), corrected with 1% urea and 30% concentrate. The experiment consisted of four experimental periods of 14 d each, with the feed, leftovers and feces sampled on the last four days of each period. The marker concentrations in the feed, leftovers and fecal samples were estimated by an in situ ruminal incubation procedure with a duration 240 h. The relationship between the intake and excretion of the markers was obtained by adjusting a simple linear regression model, independently from the treatment (diets) fixed effects and Latin squares. For both the sheep and goats, a complete recovery of the iDM and iNDF markers was observed (p>0.05), indicating the absence of LTB for these markers. However, the iADF was not completely recovered, exhibiting an LTB of -9.12% (p<0.05) in the sheep evaluation and -3.02% (p<0.05) in the goat evaluation.

The inclusion of detoxified castor bean meal in sugarcane silage increases the carcass weight and the content of unsaturated fatty acids in lamb meat.

The aim of this study was to evaluate the performance and meat quality of lambs fed sugarcane silage containing increasing levels of detoxified castor bean meal (DCBM). Twenty-four Santa Inês male sheep were used, with initial body weight of 25.34 ± 2.57 kg, distributed in a completely randomized design with four treatments (0, 70, 140, and 210 g/kg of DCBM in the sugarcane silage). Intake and digestibility of dry matter (DM) and crude protein (CP) were positively influenced (P < 0.05) by the levels of DCBM. There was a linear increase (P < 0.05) on the intake and digestibility of neutral detergent fiber with the inclusion of DCBM. The daily and total gains showed a quadratic effect (P < 0.05) with the inclusion of DCBM, with a maximum point of 241.64 g/d and 18.12 kg at the level of 161.9 g/kg of DCBM. There was a linear increase (P < 0.01) on the hot carcass with the inclusion of DCBM. The inclusion of DCBM did not influence (P > 0.05) the levels of total lipids (2.1%) in lamb meat, but the levels of oleic acid (C18:1 n-6c) and total monounsaturated fatty acids presented a quadratic effect (P < 0.05). It is recommended to include 160 g/kg of detoxified castor bean meal in sugarcane silage.

Metabolizable Protein: 1. Predicting Equations to Estimate Microbial Crude Protein Synthesis in Small Ruminants.

Microbial crude protein (MCP) produced in rumen could be estimated by a variety of protocols of experimental sampling and analysis. However, a model to estimate this value is necessary when protein requirements are calculated for small ruminants. This model could be useful to calculate rumen degradable protein (RDP) requirements from metabolizable protein (MP). Then, our objective was to investigate if there is a difference in MCP efficiency between sheep and goats, and to fit equations to predict ruminal MCP production from dietary energy intake. The database consisted of 19 studies with goats (n = 176) and sheep (n = 316), and the variables MCP synthesis (g/day), total digestible nutrients (TDN), and organic matter (OM) intakes (g/day), and OM digestibility (g/kg DM) were registered for both species. The database was used for two different purposes, where 70% of the values were sorted to fit equations, and 30% for validation. A meta-analytical procedure was carried out using the MIXED procedure of SAS, specie was considered as the fixed dummy effect, and the intercept and slope nested in the study were considered random effects. No effect of specie was observed for the estimation of MCP from TDN, digestible Organic Matter (dOM), or metabolizable energy (ME) intakes (P > 0.05), considering an equation with or without an intercept. Therefore, single models including both species at the same fitting were validated. The following equations MCP (g/day) = 12.7311 + 59.2956 × TDN intake (AIC = 3,004.6); MCP (g/day) = 15.7764 + 62.2612 × dOM intake (AIC = 2,755.1); and MCP (g/day) = 12.7311 + 15.3000 × ME intake (AIC = 3,007.3) presented lower values for the mean square error of prediction (MSEP) and its decomposition, and similar values for the concordance correlation coefficient (CCC) and for the residual mean square error (RMSE) when compared with equations fitted without an intercept. The intercept and slope pooled test was significant for equations without an intercept (P < 0.05), indicating that observed and predicted data differed. In contrast, predicted and observed data for complete equations were similar (P > 0.05).

Carcass and meat traits of goats fed diets containing cottonseed cake.

The cottonseed cake has the necessary nutritional characteristics to be able to substitute the traditional ingredients (such as soybean meal) and reduce the costs of the diet. However, it is necessary to determine the best level of inclusion of cottonseed cake in the diets of fattening goats to improve meat production and quality. The objective of this study was to evaluate carcass and meat traits of feedlot goats fed diets containing cottonseed cake replacing soybean meal (33 %, 66 % and 100 %). Thirty-two uncastrated Boer crossbred goats (4 months old, 16  ±  2 kg initial body weight) were used in a completely randomized experimental design. Replacing soybean meal with cottonseed did not compromise ( P > 0.05 ) slaughter weight, carcass traits (dressing percentage, loin-eye area and back-fat thickness), primal cuts or carcass morphometric measurements; moisture, protein, or total lipid contents of meat; or the physicochemical traits of color ( L * , a * and b * coordinates), pH, shear force, and cooking loss. However, there was a reduction ( P = 0.001 ) in the mineral matter content (from 1.08 % to 0.97 %) and an increase ( P = 0.006 ) in the cholesterol content (from 50.85 to 70.55 mg/100 g of meat) of the meat as the dietary levels of cottonseed cake were increased. Based on the results of production and meat quality, we recommend using cottonseed cake as an alternative protein source to replace up to 100 % of soybean meal in feedlot goat diets.

Selection of cassava varieties for biomass and protein production in semiarid areas from Bahia / Seleção de variedade de mandioca para produção de biomassa e proteína no semiárido baiano

The selection of cassava varieties for cultivation in semiarid regions constitutes an alternative to generate income and for animal feed. The objective of this study was to evaluate the potential for biomass and protein production of seven cassava varieties in semiarid area from Bahia. Eleven agronomic shoot (SH) and root (ROT) traits, as well as crude protein (CP), were evaluated using a randomized block design with four replications in Senhor do Bonfim (BA). Principal component analysis (PCA) and correlations analysis were carried out among the traits. Significant differences were found among varieties for all traits except for dry matter content of the shoots. Important variations were identified for crude protein content (17.9 to 25.13%), root yield (8.17 to 19.79 t.ha-1), yield of the upper third of the aerial part (from 9.36 to 15.89 t.ha-1) and dry matter yield of the shoot (1.99 to 3.14 t.ha-1), crude protein content in the shoot (0.37 to 0.64 t.ha-1) and roots (from 0.12 to 0.37 t.ha-1). According to the PCA the first two components accounted for over 77% of the variation, and traits related to yield were the main sources of diversity among the cassava varieties. Most of the correlations were positive and favorable for the selection of the most suitable varieties for production in semiarid regions. Although, grouping the cassava varieties based on PCA was not possible, varieties 'BRS Verdinha' showed good potential for protein production and variety 'Izabel de Souza' as a producer of biomass (shoot and roots).

A seleção de variedades de mandioca para cultivo nas regiões semiáridas constitui-se uma alternativa para geração de renda e uso na alimentação animal. O trabalho teve por objetivo avaliar o potencial de produção de biomassa e proteína de sete variedades de mandioca no semiárido baiano. Foram avaliadas 11 características agronômicas da parte aérea e raiz, bem como teor de proteína bruta (PB), utilizando delineamento experimental de blocos casualizados com quatro repetições. Também foram realizadas a análise de componentes principais e de correlações entre características. Foram identificadas diferenças significativas entre as variedades para todas as características exceto para teor de matéria seca na parte aérea. Variações importantes foram identificadas para teor de proteína (17,90-25,13%), produtividade de raízes (8,17 a 19,79 t.ha-1), matéria fresca (9,36-15,89 t.ha-1) e seca da parte aérea (1,99-3,14 t.ha-1), proteína na parte aérea (0,37-0,64 t.ha-1) e na raiz (0,12-0,37 t.ha-1). De acordo com a análise de componentes principais os dois primeiros responderam por mais de 77% da variação dos dados, e as características relacionadas à produtividade foram as principais fontes de diversidade entre as variedades de mandioca. A maioria das correlações foram positivas e favoráveis à seleção de variedades mais aptas para produção no semiárido. Embora, não tenha sido possível agrupar as variedades de mandioca com base na análise de componentes principais, foi possível verificar o potencial da variedade BRS Verdinha para produção de proteína e da variedade Izabel de Souza como produtora de biomassa tanto parte aérea quanto raiz.