RESUMO
A new species belonging to genus Eupatorus Burmeister, 1847, Eupatorus pyros Prandi Grossi, new species (Scarabaeidae: Dynastinae: Dynastini: Chalcosomina), from southwestern China is described and illustrated. The new species is close to E. birmanicus Arrow, 1908. A distribution map and an identification key for the Eupatorus siamensis species-group are provided.
Assuntos
Besouros/classificação , Animais , ChinaRESUMO
The taxa of the genus Megasoma Kirby, 1825 (Coleoptera, Scarabaeidae, Dynastinae) related to M. gyas (Jablonsky in Herbst, 1785) are revised. Megasoma (M.) gyas is recognized as a monotypic species restricted to the Caatinga biome of northeastern Brazil. Megasoma gyas rumbucheri Fischer, 1968, is considered as a new synonym of M. gyas. The "long-horned M. gyas" is recognized as a separate polytypic species M. (M.) typhon (Olivier, 1789) with the nominative subspecies occurring through the Mata Atlântica biome of Brazil, from Bahia to São Paulo states and M. (M.) typhon prandii Milani, 2008 restricted to a small area in the state of Santa Catarina, South Brazil. Megasoma gyas porioni Nagai is considered as a new synonym of M. typhon typhon. The "short-horned M. gyas" occurring in Minas Gerais, São Paulo, and southwestern Bahia, is recognized as a separate new species and described as M. (M.) hyperionsp. nov. The paper includes an historical research and the redescriptions of the other nominal species of the genus. Distribution maps and a key to species in the M. (M.) gyas species group (males and females) are also provided.
RESUMO
Capillary electrophoresis (CE) was applied to the quantitation of dermatan sulfate (DS) and chondroitin sulfate (CS) as related substances in sodium heparin. The method is based on the selective digestion of either CS and DS contained in the main drug heparin, by using chondroitinase ABC (specific for both DS and CS) and chondroitinase AC (specific for only CS). The unsaturated disaccharides released after exhaustive digestion, can be separated by CE using a 110mM phosphate buffer, pH 3.5 as the background electrolyte in a fused silica capillary (64.5cmx50mum i.d.) at 40 degrees C and -30kV. Since the level of each disaccharide released upon enzymatic digestion corresponds to its content in the native glycosaminoglycan, the amount of CS and DS was determined by proportion with the released disaccharides. In particular, DeltaUA-->GalNAc-4S Na(2) and DeltaUA-->GalNAc-6S Na(2) were selected for quantitation of CS and DS because of their significant response and short migration time (less than 7min).The method was validated for linearity, accuracy, precision and it showed to be able in detecting selectively, DS and CS at impurity level (LOD 0.01%, w/w). The proposed CE approach was finally applied to real samples. The results obtained were found in excellent correlation with those achieved by the analysis of the same samples using the official USP method based on high performance anion exchange chromatography (HPAEC) with pulsed amperometric detector.