RESUMO
Glyphosate is the most sprayed pesticide across the globe. Its toxicity to non-target marine organisms has recently piqued the scientific community's interest. Therefore, the purpose of this study is to investigate the potentially toxic effects of glyphosate on scallops, an ecologically and economically important bivalve group. To do that, specimens of the smooth scallop Flexopecten glaber were exposed to different concentrations (10, 100, and 1000 µg L-1) of the technical-grade glyphosate acid (GLY) for 96 h. The detrimental effects of this pollutant were assayed at cellular and tissular levels. The obtained results showed that the GLY was able to induce oxidative stress in the gills and the digestive gland of F. glaber as revealed by the enhanced hydrogen peroxide (H2O2), protein carbonyls (PCO), malondialdehyde (MDA), and lipid peroxides (LOOH) levels and the altered antioxidant defense system (the glutathione GSH content and the superoxide dismutase (SOD) activity). Additionally, GLY was found to alter the fatty acid profile, to exert a neurotoxic effect through the inhibition of the acetylcholinesterase (AChE) activity, and to provoke several histopathological damages in the two organs studied. The obtained results revealed that the pure form of GLY may exert toxic effects on F. glaber even at relatively low concentrations.
Assuntos
Pectinidae , Poluentes Químicos da Água , Acetilcolinesterase/metabolismo , Animais , Antioxidantes/metabolismo , Glutationa/metabolismo , Glicina/análogos & derivados , Peróxido de Hidrogênio , Lipídeos , Estresse Oxidativo , Pectinidae/metabolismo , Superóxido Dismutase/metabolismo , Poluentes Químicos da Água/toxicidade , GlifosatoRESUMO
The aim of this study is to elucidate the toxicity induced by PbCl2, administered in graded doses (1; 2.5; 5 mg/L), in the redox state, in the fatty acid composition and in the histological structure of Mactra corallina digestive glands. Our findings showed a progressive accumulation of Pb in the M. corallina digestive glands in all treated groups. After 5 days of treatment with PbCl2, an increase in H2O2, MDA, PCO, GSH, NPSH and MT levels was observed in the digestive glands of treated groups. Moreover, activities of antioxidant enzymes, such as GPx and CAT, increased while SOD activity decreased in all treated groups, indicating a failure of the antioxidant defense system. Furthermore, the cholinergic function was evaluated by assessing the acetylcholinesterase activity, which was inhibited in all the treated digestive glands compared to the control group. In our experiment, the levels of n-3 (Omega-3) and n-6 (Omega-6) polyunsaturated fatty acids were greatly altered in the groups treated with 5 mg/L of PbCl2 (p < 0.001). Indeed, DHA and EPA decreased significantly in the digestive glands treated with 2.5 and 5 mg/L of PbCl2, respectively. On the other hand, under exposure to the medium and high doses (2.5 and 5 mg/L), arachidonic acid (ARA) and linoleic acid (LA) significantly increased (p < 0.001). These changes in PUFA were confirmed by significant modifications in the polyene and peroxidation indices. The histological findings confirmed the biochemical results.HighlightsIntegrated parameters were used to explore the toxic effect of PbCl2 graded doses in Mactra corallina digestive gland.Metals accumulation in digestive glands of M. corallina was greatly dependent to dose of PbCl2.Metabolic and macromolecules damaging of clams digestive glands were more sensitive at higher PbCl2 doses.Fatty acids composition especially polyunsaturated fatty acids (PUFA) and essentials fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in clams digestive glands were intensively altered at higher PbCl2 doses.Inflammation responses revealed in studied tissue.
Assuntos
Bivalves , Ácidos Graxos Ômega-3 , Acetilcolinesterase , Animais , Ácidos Graxos , Peróxido de Hidrogênio , Chumbo/toxicidade , OxirreduçãoRESUMO
Marine heavy metal pollution is a worldwide serious issue. Like almost all Mediterranean lagoons, the Bizerte lagoon is highly urbanized and suffers from intensive anthropogenic pressure. In the present study, we screened the metal contamination and biomarker responses in the smooth scallop Flexopecten glaber inhabiting this vulnerable ecosystem. To this end, the concentrations of six heavy metals (HM) (i.e., Cu, Pb, Zn, Cd, Hg, and Fe) and a panel of biochemical endpoints including malondialdehyde (MDA), metallothioneins (MT), reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were determined in the gills and digestive gland across seasons (warm and cold) and sites (S1 and S2). The distribution of almost all analyzed metals in F. glaber tissues varied significantly between sites, seasons, and organs. The highest levels were recorded at S2 during the warm period. Moreover, the digestive gland was found to accumulate greater concentrations of HM than the gills. Marked spatio-temporal variations were also observed for oxidative stress biomarkers, mainly in the gills, while the digestive gland seems to be rather sensitive to seasonal variability. Particularly, we noticed that among the used biomarkers, MT did not show significant variations in the two tested organs across seasons and sites. From the obtained results, F. glaber appears as a sensitive organism to anthropogenic metal contamination and can be proposed as a promising bioindicator species for marine pollution.
Assuntos
Metais Pesados , Pectinidae , Animais , Biomarcadores , Ecossistema , Monitoramento Ambiental/métodos , Metalotioneína , Metais Pesados/análiseRESUMO
This study aims to verify the relevance of Brachidontes pharaonis to assess the ecotoxicological status of polluted sites. For this, the levels of some heavy metals (i.e. Zn, Cu, Pb, and Cd) and a battery of biomarkers including metallothionein (MT), malondialdehyde (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were assessed in mussels collected from the harbor of Rades (North), and the harbor of Zarzis (South). Moreover, abiotic parameters including temperature, salinity, pH, and dissolved oxygen were assessed. Results from the ICP-OES showed that the southern population exhibited a higher metal pollution index with significantly higher Zn, Cu, and Pb concentrations. Moreover, the specimens from Zarzis displayed significantly higher levels of MDA, MT, GSH, GPx, SOD, and CAT reflecting higher levels of oxidative and chemical stress. These results emphasize the potential utility of B. pharaonis for the monitoring of heavily impacted sites.
Assuntos
Bivalves , Metais Pesados , Mytilidae , Poluentes Químicos da Água , Animais , Biomarcadores Ambientais , Bioacumulação , Chumbo , Metais Pesados/toxicidade , Metais Pesados/análise , Bivalves/metabolismo , Estresse Oxidativo , Catalase/metabolismo , Metalotioneína/metabolismo , Glutationa Peroxidase/metabolismo , Superóxido Dismutase/metabolismo , Biomarcadores/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/análiseRESUMO
Parasitic copepod Lernaeocera lusci is a common mesoparasite of the hake Merluccius merluccius. Although widely distributed throughout the Mediterranean, little is known about this pathogen. The current study was designed to assess the impact of different L. lusci infection loads on lipid classes and their fatty acid (FA) composition in both parasite and the host organs (gills, liver, and muscle). Results showed a significant decrease in total lipid, neutral lipid (NL), and polar lipid (PL) contents in all analyzed host's organs in relationship with parasite intensity. Gills appeared to be the most impacted organ under the lowest parasite intensity (loss of 50% of NL and PL amounts). At the highest parasitic infection, a loss of about 80% of lipid moieties was recorded in all analyzed organs. Simultaneously, no significant differences were found for the parasite reflecting its ability to sustain an appropriate lipid amount required for its survival and development. Significant changes in the FA composition were recorded in both host and parasite. Particularly, we have noticed that for L. lusci, the intraspecific competition has resulted in an increased level of some essential FA such as C22:6n-3 (docosahexaenoic acid, DHA), C20:5n-3 (eicosapentaenoic acid, EPA), and C20:4n-6 (arachidonic acid, ARA). This probably reflects that in addition to a direct host FA diversion, L. Lusci can modulate its FA composition by increasing the activity of desaturation. Within the host, liver PL appeared to be the less impacted fraction which may mirror an adaptive strategy adopted by the host to preserve the structural and functional integrity of this vital organ.
Assuntos
Copépodes/química , Gadiformes/parasitologia , Metabolismo dos Lipídeos , Lipídeos/classificação , Doenças Parasitárias em Animais/parasitologia , Animais , Copépodes/fisiologia , Gadiformes/fisiologia , Interações Hospedeiro-Parasita , Lipídeos/química , Doenças Parasitárias em Animais/metabolismoRESUMO
Coastal lagoons are among the most vulnerable ecosystems as they are often exposed to different anthropogenic activities. The Polychaetes, which are dominant components in macrobenthic community, are particularly exposed to contamination. The current study was designed to assess and compare the sensitivity of different polychaetes species towards urban pollution. To do this, three polychaete species: Perinereis cultrifera, Diopatra neapolitana, and Marphysa sanguinea, were collected from the Tunis South Lagoon during summer 2013. A set of biomarkers indicative of genotoxicity (DNA damage), biotransformation, and oxidative stress (glutathione S-transferase, GST) as well as immune response (cyclooxygenase activity (COX), lysozyme activity, and nitric oxide level (NOx)), was used. The results revealed that D. neapolitana and P. cultrifera exhibited higher genetic alteration and GST activity and more prominent immune response when compared with M. sanguinea. These findings denote of the higher sensitivity of D. neapolitana and P. cultrifera to urban pollution and suggest their possible use in environmental biomonitoring programs.
Assuntos
Poliquetos , Poluentes Químicos da Água , Animais , Biomarcadores/metabolismo , Ecossistema , Monitoramento Ambiental , Estresse Oxidativo , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Mercury (Hg) is among the most deleterious contaminant in the aquatic environment and presents a serious risk to humans and ecosystems. This study evaluated the effects of Hg on oxidative stress biomarkers, DNA integrity and histological structure of the respiratory tree of Holothuria forskali exposed to different concentrations of mercury chloride HgCl2 (0.04, 0.08 and 0.16 mg L-1) for 96 h. Exposure of H. forskali to Hg led to oxidative stress with an increase in Malondialdehyde (MDA), hydrogen peroxide (H2O2), advanced oxidation protein product (AOPP) and protein carbonyls (PCO) levels in the treated groups. Alteration of the antioxidant system was also confirmed by the significant increase in glutathione (GSH), nonprotein thiol (NPSH) and vitamin C contents. Moreover, the enzymatic activity of superoxide dismutase (SOD), Glutathione peroxidase (GPX) and Catalase (CAT) increased significantly. Our research revealed that total Metallothionein (MTs) content enhanced in a dose-dependent manner. Interestingly, the exposure to this metal provoked a decrease in Acetylcholinesterase (AChE) activity. Hg genotoxicity was further evidenced by a random DNA degradation that was observed in the treated groups. The histopathological findings confirmed the biochemical results. Overall, our results indicated that mercury-induced genotoxicity, oxidative damage and histopathological injuries in the respiratory tree of H. forskali.
Assuntos
Cloreto de Mercúrio/toxicidade , Metalotioneína/genética , Mutagênicos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Relação Dose-Resposta a Droga , Holothuria/efeitos dos fármacos , Cloreto de Mercúrio/administração & dosagem , Testes de Mutagenicidade , Mutagênicos/administração & dosagem , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Sistema Respiratório/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos , Poluentes Químicos da Água/administração & dosagemRESUMO
The aim of this study was to analyze the biochemical alterations in the gills of Venus verrucosa under exposure for 6 days to three doses of lambda-cyhalothrin (λ-cyh) (100, 250, and 500 µg L-1). Malondialdehyde, lipid hydroperoxide, and hydrogen peroxide levels in the gills of treated groups increased. λ-Cyh exposure significantly increased the protein carbonyl and reduced glutathione levels in the gills of all treated groups. Moreover, the activities of superoxide dismutase and glutathione peroxidase were increased. In our study, the polyunsaturated fatty acid (FA), omega-6, eicosapentaenoic acid (C20:5n-3), and docosahexaenoic acid (C22:6n-3) were increased in the treated groups. A significant decrease in the saturated FAs, omega-3, and arachidonic acid (C20:4n-6) levels was observed. The content of monounsaturated FA was changed in the groups treated with 100 and 250 µg L-1 of λ-cyh. As a corollary, desaturase and elongase activities were significantly increased. Our study provides evidence of the underlying toxic mechanism of λ-cyh and its capacity to create oxidative stress and revealed that FA profiling is a new approach for elucidating the λ-cyh toxicity.
Assuntos
Antioxidantes/metabolismo , Ácidos Graxos/metabolismo , Brânquias/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Nitrilas/farmacologia , Praguicidas/farmacologia , Piretrinas/farmacologia , Animais , Relação Dose-Resposta a DrogaRESUMO
Mercury is one of the most harmful pollutant that threat marine biota. This study assessed the Hg impact on the fatty acid (FA) composition and the antioxidant statues in Holothuria forskali body wall tissue. Specimens were exposed to HgCl2 graded doses (40, 80 and 160⯵gâ¯L-1) for 96â¯h. A decrease in linoleic, arachidonic and eicosapentaenoic acid levels and an increase of docosahexaenoic acid were mainly observed at the nominal tested dose. The exposure to the upper dose promoted oxidative stress with an increase of malondialdehyde, hydrogen peroxide, advanced oxidation protein product, glutathione and non-protein thiols levels. Moreover, a decrease in catalase and an increase in superoxide dismutase and glutathione peroxidase activities were observed. Yet, an increase of the metallothionein level was registered in all treated groups. This study confirmed the Hg toxicity on the redox statue of H. forskali and highlighted the usefulness of the FA composition as an early sensitive bioindicators.
Assuntos
Antioxidantes/metabolismo , Ácidos Graxos/metabolismo , Holothuria/efeitos dos fármacos , Cloreto de Mercúrio/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Biomarcadores/metabolismo , Holothuria/metabolismo , Mar Mediterrâneo , Cloreto de Mercúrio/análise , Oxirredução , Água do Mar/química , Tunísia , Poluentes Químicos da Água/análiseRESUMO
Teleost fish are ectothermic vertebrates. Their metabolism, physiology and behavior rely on the external temperature. This study, on the retina of the sea bass Dicentrarchus labrax, reports on the impact of temperature on the fatty acid composition and mRNA abundance of key enzymes of lipid metabolism: fatty acid desaturase-2 (FADS2), fatty acid elongase-5 (ELOVL5), sterol regulatory element-binding protein-1 (SREBP-1), triglyceride lipase and phospholipase A2 (PLA2). We also report on the effects on the photopigment molecule rhodopsin and on enzymes of the melatonin synthesis pathway, namely arylalkylamine N-acetyltransferases 1a and 1b and acetylserotonin methyltransferase. Juvenile fish were placed for 30 days at 18, 23 or 28 °C. At 23 °C, the fatty acid composition of D. labrax retina showed, as generally reported for the retina of other fish species, particularly high amounts of docosahexaenoic (DHA), palmitic and oleic acids. The fatty acids composition was not significantly (P > 0.05) altered between 23 and 28 °C, but did increase at 18 °C compared to 23 and 28 °C. At 18 °C there were noticeable increases in total DHA, ecosapentaenoic, arachidonic, oleic, linoleic, palmitoleic and stearic acids. A negative correlation was found in the abundance of neutral (NL) vs. polar (PL) lipids: 18 °C induced an increase in NL and a decrease in PL, while 28 °C induced higher PL with decreased NL. In NL the changes affected mainly triglycerides. FADS2 and ELOVL5 mRNA abundance decreased from 18° to 28 °C while SREBP-1 and triglyceride lipase mRNA remained stable. Conversely PLA2 mRNA was more abundant at 23 than at 18 and 28 °C. Temperature increased and decreased rhodopsin mRNA abundance, at 28 °C and 18 °C respectively, while there was no effect on mRNA from the melatonin synthesis enzymes. In conclusion the data indicate a temperature induced redistribution of fatty acids among the lipid classes that might affect the physical properties of the plasma membranes as well as functions associated with photoreception or generation of intracellular second messengers. In addition, the results suggest that temperature targets only the proteins and activities of retinal melatonin production. This study opens new lines of investigation related to the role temperature and fatty acids play in fish visual perception. They are relevant in the context of the global warming of seas affecting both the wild and the aquaculture species.
Assuntos
Ácidos Graxos/metabolismo , Metabolismo dos Lipídeos , Melatonina/metabolismo , Retina/metabolismo , Rodopsina/metabolismo , Animais , Bass , Modelos Animais , Retina/citologia , TemperaturaRESUMO
Despite international recognition as an important coastal wetland, Tunis Lagoon is still subjected to anthropogenic pressures. This article provides valuable data on the spatio-temporal distribution, toxicity, and origins of polycyclic aromatic hydrocarbons (PAHs) in the Tunis Lagoon complex. PAHs' concentrations were measured in Marphysa sanguinea body and excrements, as well as in surface sediments. Total mean PAHs' concentrations reached a maximum of 2398 ng/g dry weight (DW) in sediments, 1007.19 ng/g DW in M. sanguinea, and 2602.05 ng/g DW in excrements. Diagnostic PAHs' ratios were used to determine whether PAHs' origins were pyrogenic or petrogenic. Our data showed a predominance of PAHs with a pyrogenic source. Principal component analysis showed PAHs isolated from polychaetes clearly separated from those measured in sediment and excrement. We believe that sediments are not the main source of bioaccumulation by M. sanguinea. Moreover, the toxicity of PAHs in sediments is moderate to high for benthic organisms.
Assuntos
Poliquetos , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Hidrocarbonetos Policíclicos Aromáticos/análise , Sedimentos Geológicos/análise , Monitoramento Ambiental , Poluentes Químicos da Água/análiseRESUMO
This work assessed the impact of polycyclic aromatic hydrocarbons (PAHs) on the polychaeta Marphysa sanguinea in Tunis Lagoon. Highest PAHs concentrations were accumulated at station E with maximum Σ PAH of 6028,87 ng/g DW. Changes in animal physiology were clearly related to bioaccumulated PAH. In fact, high levels of immune biomarkers (cyclooxygenase [COX] and lysozyme activity with maximum of 44631,10 FU/mn/mg protein and 0,017 lysozyme activity/mn/mg protein, respectively) were recorded at stations B and E. Triacylglycerol (TAG), the energy source, was lowest at the most polluted stations (E and B), while phospholipids (PL) were highest at the control station. Statistical analysis revealed a probable effect of both low and high molecular weight PAHs on variations in energy storage lipids (TAG and sterol and wax esters [SE/WE]) and membrane lipids, particularly PL. Our results encourage the use of M. sanguinea to assess pollution levels in coastal ecosystems.
Assuntos
Poliquetos , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Hidrocarbonetos Policíclicos Aromáticos/análise , Poliquetos/metabolismo , Bioacumulação , Ecossistema , Prostaglandina-Endoperóxido Sintases/metabolismo , Muramidase/metabolismo , Biomarcadores/metabolismo , Dano ao DNA , Fosfolipídeos , Triglicerídeos , Lipídeos de Membrana , Esteróis/análise , Ésteres , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análiseRESUMO
Engineered iron nanoparticles are widely used in environmental remediation, yet their potential toxic effects on marine biota remain poorly elucidated. This study aimed to gain insight into the nanoscale zero-valent iron (NZVI) toxicity mechanisms for marine invertebrates. Aside from the effect on oxidative status and histopathology, the effect of NZVI on lipid metabolism in bivalves was studied for the first time. To this end, specimens of Flexopecten glaber were exposed to ascending concentrations (0.5, 1, and 1.5 mg/L) of NZVI for 96 h. Results illustrate differential patterns of iron accumulation in the gills and the digestive gland. By increasing NZVI concentrations, the total iron level tended to markedly increase in the gills and decrease in the digestive gland, reaching 132 and 37.6 µg/g DW, respectively, in the specimens exposed to 1.5 mg/L. Biochemical and cellular biomarkers highlighted that NZVI caused oxidative stress (measured as hydrogen peroxide, malondialdehyde, and advanced oxidation protein product levels) and alterations of antioxidant defense systems, including reduced glutathione, non-protein thiol, glutathione peroxidase, superoxide dismutase, and catalase. Modulation of lipid metabolism with changed fatty acid compositions (mainly an increase in the saturation and a decrease in unsaturation levels) was also observed in both gills and digestive gland. Moreover, several histological damages, including lipofuscin accumulation, infiltrative inflammations, and digestive tubule alterations, were observed in the two studied organs, providing supplementary evidence regarding the toxic effect of NZVI. This study adds to the growing body of evidence pointing to the hazardous impacts of iron NPs on aquatic ecosystems.
Assuntos
Nanopartículas Metálicas , Pectinidae , Animais , Ferro/química , Catalase/metabolismo , Antioxidantes/metabolismo , Glutationa Peroxidase/metabolismo , Peróxido de Hidrogênio/metabolismo , Ácidos Graxos/farmacologia , Ecossistema , Produtos da Oxidação Avançada de Proteínas/metabolismo , Produtos da Oxidação Avançada de Proteínas/farmacologia , Lipofuscina/metabolismo , Lipofuscina/farmacologia , Nanopartículas Metálicas/toxicidade , Nanopartículas Metálicas/química , Estresse Oxidativo , Malondialdeído/metabolismo , Superóxido Dismutase/metabolismo , Glutationa/metabolismo , Biomarcadores/metabolismo , Compostos de SulfidrilaRESUMO
In this study, the potential hazardous impacts of the technical grade glyphosate acid (GLY) and its commercial formulation roundup (RD®) were evaluated for the first time on holothurians. To do this, redox status, fatty acid (FA) profile, and histopathology aspects were assessed in the respiratory tree tissue of the sea cucumber Holothuria forskali following short-term exposure (96 h) to a series of concentrations (10, 100 and 1000 µg L-1) of GLY and RD® (glyphosate acid equivalent). Our results showed that both GLY and RD® promoted oxidative stress highlighted with an increase of hydrogen peroxide (H2O2), malondialdehyde (MDA), lipid peroxides (LOOH) and advanced oxidation protein product (AOPP) levels in all treated groups. In addition, both glyphosate forms were found to perturb the FA composition. However, changes in saturated (SFA) and polyunsaturated (PUFA) including some essential FA (LA, ARA, EPA and DHA) revealed differential compensatory/adaptive processes in H. forskali depending on the treatment. GLY and RD® were also found to modulate the enzymatic (glutathione S-transferases, glutathione peroxidase and catalase) and non-enzymatic (reduced glutathione and ascorbic acid) antioxidant defense status. Taken together, our results revealed that the commercial formulation produced more pronounced effects on H. forskali respiratory tree than the pure form. This finding was further confirmed by the histological observations.
Assuntos
Herbicidas , Holothuria , Pepinos-do-Mar , Animais , Biomarcadores , Glicina/análogos & derivados , Herbicidas/toxicidade , Peróxido de Hidrogênio , Árvores , GlifosatoRESUMO
Acrylamide (ACR), a ubiquitous agent, has various chemical and industrial applications, and it is found in backed or fried carbohydrate-rich food. It has been related to multiple toxicological effects, and it causes high cytotoxicity through oxidative stress. The present study aimed to investigate the potential effect of ACR toxicity administered at different concentrations (5, 10, and 20 mg/L), during 5 days, in order to evaluate the fatty acid (FA) composition and redox state in the digestive gland of Mactra corallina. The results showed, in ACR-treated clams, a significant increase in malondialdehyde, hydrogen peroxide, protein carbonyl, and metallothionein levels, as well as an alteration of the enzymatic (superoxide dismutase, glutathione peroxidase, and catalase) and non-enzymatic (reduced glutathione and ascorbic acid) antioxidant status. However, acetylcholinesterase activity was inhibited in a concentration-dependent manner. In our experiment, the n-3 (Omega-3) and n-6 (Omega-6) polyunsaturated fatty acid levels were significantly changed in all ACR-treated groups. A decrease in eicosapentaenoic acid (C20:5n-3, EPA) and docosahexaenoic acid (C22:6n-3, DHA) was observed in 10-mg/L and 20-mg/L ACR-treated groups. Nevertheless, arachidonic acid (C20:4n-6, ARA) and its precursor linoleic acid (C18:2n-6, LA) were increased. Besides oxidative stress parameters, FA composition may be an additional tool for assessing ACR contamination.