Label-Free Detection of Unbound Bilirubin and Nitrophenol Explosives in Water by a Mechanosynthesized Dual Functional Zinc Complex: Recognition of Picric Acid in Various Common Organic Media.

High levels of unconjugated bilirubin (UB) in serum lead to asymptomatic and neonatal jaundice and brain dysfunctions. Herein, we have reported the detection of UB at as low as 1 µM in an aqueous alkaline medium using a Zn(II) complex. The specificity of the complex has been validated by the HPLC in the concentration window 6-90 µM, which is rare. The sensory response of the probe at physiological pH against nitro explosives developed it as an instant-acting fluorosensor for picric acid (PA) and 2,4-dinitrophenol (2,4-DNP). Spectroscopic titration provided a binding constant of 4×105  M-1 with PA. The naked eye detection was found to be 15 µM. The solid-state photoluminescent nature of the complex enabled it for PA sensing in the solid phase. Interestingly, the probe remained fluorescent in various volatile and non-volatile organic solvents. As a result, it can also detect PA and 2,4-DNP in a wide range of common organic media. NMR studies revealed the coordination of PA, 2,4-DNP, and UB to the Zn(II) center of the probe, which is responsible for the observed quenching of the probe with the analytes.

Supplemental phosphorus can be completely replaced with microbial phytase in White Leghorn layer diets.

1. A study was conducted to assess the possibility of totally replacing supplemental phosphorus sources in White Leghorn (WL) layer diets (aged 28 to 45 weeks of age) with microbial phytase supplementation. One thousand commercial layers (HyLine White) of 28 weeks of age were housed in California cages fitted in open-sided poultry shed at the rate of 20 layers in each replicate. Ten replicates were randomly allotted to each treatment, and the respective diet was fed from 28 to 45 weeks of age.2. A control diet (CD) containing the recommended levels of non-phytate phosphorus (3.6 g/kg NPP) and four other test diets (2-5) having sub-optimal levels of NPP (2.4, 2.0, 1.6 and 1.2 g/kg), but with supplemental microbial phytase (600 FTU/kg) were prepared and fed for the trial duration.3. The layers fed with lower levels of NPP with phytase had the same laying performance as the group fed the CD. Egg production, feed efficiency, egg mass, shell defects, egg density, shell weight, shell thickness, ash content and breaking strength of the tibia and sternum were not affected by feeding the lowest concentration of NPP (1.2 g/kg) plus microbial phytase.4. Phytase supplementation in diets with sub-optimal levels of NPP (2.4, 2 and 1.6 g/kg) significantly improved the Haugh unit score compared to those fed the CD.5. It was concluded that supplemental phosphorus can be completely replaced with microbial phytase (600 FTU/kg) in a diet without affecting egg production, shell quality or bone mineral variables in WL layers (28 to 45 weeks).

Taylor-Gorilla troops optimized deep learning network for surface roughness estimation.

In order to guarantee the desired quality of machined products, a reliable surface roughness assessment is essential. Using a surface profile metre with a contact stylus, which can produce accurate measurements of surface profiles, is the most popular technique for determining the surface roughness of machined items. One of the limitations of this technique is the work piece surface degradation brought on by mechanical contact between the stylus and the surface. Hence, in this paper, a roughness assessment technique based on the suggested Taylor-Gorilla troops optimizer-based Deep Neuro-Fuzzy Network (Taylor-GTO based DNFN) is proposed for estimating the surface roughness. Pre-processing, data augmentation, feature extraction, feature fusion, and roughness estimation are the procedures that the suggested technique uses to complete the roughness estimate procedure. Roughness estimation is performed using DNFN that has been trained using Taylor-GTO, which was created by combining the Taylor series with the Gorilla troop's optimizer. The created Taylor-GTO based DNFN model has minimum Mean Absolute Error, Mean Square Error, and RMSE of 0.403, 0.416, and 1.149, respectively.

Feed withdrawal influences egg production, egg quality and antioxidant variables in White Leghorn layers reared during the tropical summer season.

1. An experiment was conducted with White Leghorn layers (Babcock BV 300) to optimise the feed withdrawal schedule for egg-laying hens (67 to 80 weeks) reared in open-sided poultry houses during the tropical summer season.2. Four feeding schedules (1. ad libitum access to the layer feed, 2. feed withdrawal from 9 AM-5 PM, 3. 11 AM-5 PM, and 4. 1 PM-5 PM followed by free access to the layer feed) were examined. Each feeding schedule was offered to 12 replicates consisting of 12 layers housed in four adjacent cages (45.7 × 35.6 × 43.2 cm).3. Fasting the layers from 1-5 pm reduced egg production compared to layers fasting from 9 or 11 am. The FI/egg or unit egg mass was highest in layers fasted from 1-5 pm. In the 9 am-5 pm fasted group, the cloacal temperature (CT) increased significantly at 11 am and gradually reduced at 3 pm. In the 1-5 pm fasted group, the CT remained higher at 3 pm and 5 pm.4. The cell-mediated immune response in layers fasted from 9 am or 11 am was higher than those fed ad libitum or 1-5 pm fasted group. Lipid peroxidation was higher in layers 1-5 pm fasted group, and significantly lower LP was found in groups that fasted from 9 am or 11 am. In general, the activities of serum antioxidant enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase) were higher in layers that fasted from 9 am or 11 am compared to those that had ad libitum feed or fasted from 1-5 pm.5. It was concluded that the feed withdrawal should be initiated during the forenoon (9 AM/11 am-5 pm) for optimum production and to reduce oxidative stress in egg-laying hens reared during summer in the tropical region.

Feed emulsifier improves the performance and nutrient digestibility in broiler chicken fed diets without antibiotic growth promoter.

1. A study was conducted to assess the impact of supplementing-graded concentrations of emulsifier on the production performance, gut microbial count, and digestibility of nitrogen and energy in broiler chicken fed diets without AGP.2. Male broiler chicks (n = 1500; Vencobb-430), aged one-day-old, were randomly allocated into six dietary groups each with 10 replicates of 25 birds each. A maize-soybean and meat and bone meal-based basal diet without antibiotic (AGP) growth promoter served as negative control (NC). The basal diet was supplemented with BMD (AGP, bacitracin methylene disalicylate-BMD 100 g/T), which served as the positive control (PC). Emulsifier was added to the NC diets at either 250 g/ton in all phases (250-All), 250 g in starter and grower phases, and 500 g in the finisher phase (250:250:500), 250 g in starter and 500 g in both grower and finisher phases (250:500:500) and 500 g in all phases (500 g-All).3. Two broilers per replicate were slaughtered to record carcase traits and gut microbial count on day 43. There was significant improvement in body weight gain (BWG) and reduced FCR in broilers fed 250:250:500 and 250:500:500 g emulsifiers compared to other treatment groups. Carcase traits and faecal microbial count did not differ among treatments. The inclusion of BMD significantly improved nitrogen (N) digestibility compared to the NC group. The digestibility of emulsifier-supplemented groups was similar to those fed by the BMD group except for the 500-All group, which was an intermediary between NC and other emulsifier-fed groups.4. It was concluded that supplementation with emulsifier (250:250:500 or 250:500:500) without antibiotic growth promoter significantly improved FCR and body weight gain similar to broilers receiving antibiotic growth promoter, which was associated with increased ileal digestibility of N and energy.

Effect of dietary supplementation of organic and inorganic Se on performance and antioxidant response in commercial broiler chickens.

1. The study was carried out to determine the effects of supplementing organic (OG) or inorganic (IOG) Se to record the performance, immune and anti-oxidant response in broiler chickens.2. One-day-old Vencobb-400 (50.1 ± 0.84 g; n = 280) male broiler chicks were allocated randomly into seven treatments to give eight replicates containing five chicks each and housed in deep littered floor pens (76 cm × 91 cm) for a period of 42 d.3. The control diet (CD) was formulated without any Se supplementation (diet I), CD plus 0.15, 0.30 or 0.45 mg/kg OG Se (diets II, III and IV, respectively) and CD plus 0.15, 0.30 or 0.45 mg/kg IOG Se (diets V, VI and VII, respectively).4. Feed intake (FI), body weight gain (BWG), immune variables and mRNA expression profile of hepatic selenoproteins (SepW, GSHPx1, TrxR and GSHPx3) genes were determined. The BWG, FI and feed conversion ratio did not differ among various dietary treatments.5. The lipid peroxidation and activity of RBC catalase were lower (P < 0.05) in groups fed diets supplemented OG Se compared to those fed IOG Se and CD. The activity of GSH Px was higher among the groups fed diet supplemented OG Se compared to those groups fed OG Se and CD. However, supplementing diets with OG or IOG Se did not affect humoral or cell mediated immune responses.6. The expression levels of SepW were higher (P < 0.01) among the groups supplemented with OG Se. Expression levels of GSH-Px1, TrxR and GSHPx3 were higher (P < 0.01) among the groups supplemented with OG Se compared to those groups fed IOG Se or CD.7. Supplementing OG Se improved the activities of anti-oxidant enzymes and hepatic expression of selenoproteins genes in the present study. However, supplementing OG or IOG Se did not affect growth performance and immune variables in broiler chickens.

Electric Monopole Transition from the Superdeformed Band in

The electric monopole (E0) transition strength ρ^{2} for the transition connecting the third 0^{+} level, a "superdeformed" band head, to the "spherical" 0^{+} ground state in doubly magic ^{40}Ca is determined via e^{+}e^{-} pair-conversion spectroscopy. The measured value ρ^{2}(E0;0_{3}^{+}→0_{1}^{+})=2.3(5)×10^{-3} is the smallest ρ^{2}(E0;0^{+}→0^{+}) found in A<50 nuclei. In contrast, the E0 transition strength to the ground state observed from the second 0^{+} state, a band head of "normal" deformation, is an order of magnitude larger ρ^{2}(E0;0_{2}^{+}→0_{1}^{+})=25.9(16)×10^{-3}, which shows significant mixing between these two states. Large-scale shell-model (LSSM) calculations are performed to understand the microscopic structure of the excited states and the configuration mixing between them; experimental ρ^{2} values in ^{40}Ca and neighboring isotopes are well reproduced by the LSSM calculations. The unusually small ρ^{2}(E0;0_{3}^{+}→0_{1}^{+}) value is due to destructive interference in the mixing of shape-coexisting structures, which are based on several different multiparticle-multihole excitations. This observation goes beyond the usual treatment of E0 strengths, where two-state shape mixing cannot result in destructive interference.

Genome assisted probiotic characterization and application of Bacillus velezensis ZBG17 as an alternative to antibiotic growth promoters in broiler chickens.

The present study describes genome annotation and phenotypic characterization of Bacillus velezensis ZBG17 and evaluation of its performance as antibiotic growth promoter substitute in broiler chickens. ZBG17 comprises 3.89 Mbp genome with GC content of 46.5%. ZBG17 could tolerate simulated gastrointestinal juices prevalent in the animal gut. Some adhesion-associated genomic features of ZBG17 supported the experimentally determined cell surface hydrophobicity and cell aggregation results. ZBG17 encoded multiple secondary metabolite gene clusters correlating with its broad-spectrum antibacterial activity. Interestingly, ZBG17 completely inhibited Salmonella enterica and Escherichia coli within 6 h and 8 h in liquid co-culture assay, respectively. ZBG17 genome analysis did not reveal any genetic determinant associated with reported safety hazards for use as a poultry direct-fed microbial. Dietary supplementation of ZBG17 significantly improved feed utilization efficiency and humoral immune response in broiler chickens, suggesting its prospective application as a direct-fed microbial in broiler chickens.

Effect of dietary supplementation of zinc proteinate on performance, egg quality, blood biochemical parameters, and egg zinc content in White Leghorn layers.

A study was conducted to investigate the effects of zinc proteinate (Zn-P) on laying performance, egg quality, antioxidant indices, and egg zinc content in laying hens from 38 to 49 weeks of age. A total of 150 White Leghorn layers were randomly assigned to five treatments, each with six replicates with five birds per replication. Dietary treatments included a corn-soybean meal-based basal diet with no zinc addition and basal diet supplemented with Zn-P at 40, 80, 120, or 160 mg/kg of feed for 12 weeks. The analyzed zinc concentrations of the five diets were 29.5, 70.8, 110.2, 147.5, and 187.5 mg Zn/kg, respectively. Dietary Zn-P supplementation had no effect on feed intake and egg production. However, raising the zinc level improved egg weight (P < 0.01) and egg mass (P < 0.05) and lowered the feed conversion ratio (P < 0.05) during the later (46-49 weeks) period. The Zn-P supplementation also significantly (P < 0.05) increased Haugh units, egg shell strength, and shell thickness and had no influence on other egg quality parameters. Increasing zinc levels in the diet resulted in increase in egg zinc contents and serum zinc level. The serum triglyceride and LDL-cholesterol levels significantly decreased (P < 0.05) in Zn-P-supplemented groups. Supplementation of Zn-P significantly (P < 0.05) increased serum Cu-Zn-SOD activity and reduced MDA concentration. It could be concluded that dietary supplementation of higher levels of Zn-P, more than 80 mg/kg diet, significantly improved the egg zinc content, some egg quality traits, antioxidant activity, and serum zinc levels.

Skyrmion-(Anti)Vortex Coupling in a Chiral Magnet-Superconductor Heterostructure.

We report experimental coupling of chiral magnetism and superconductivity in [IrFeCoPt]/Nb heterostructures. The stray field of skyrmions with radius ≈50 nm is sufficient to nucleate antivortices in a 25 nm Nb film, with unique signatures in the magnetization, critical current, and flux dynamics, corroborated via simulations. We also detect a thermally tunable Rashba-Edelstein exchange coupling in the isolated skyrmion phase. This realization of a strongly interacting skyrmion-(anti)vortex system opens a path toward controllable topological hybrid materials, unattainable to date.

Interaction effects of sunflower oil and aflatoxin at graded levels in diet on performance, serum and tissue biochemical profile, organ weights and immuneresponse in broiler chicken.

The dietary supplementation of fat has great potential in countering the toxic effects of aflatoxin (AF) in chickens, but the issue was less researched upon. An experiment was conducted to evaluate the response of broiler chickens to graded levels of AF B1 (0, 150 and 300 ppb) and sunflower oil (SFO) (0, 1.5 and 3.0%) in the diet in a 3 × 3 factorial manner to understand their interaction effects. A total of 360 broiler chickens divided into 9 equal groups were fed the diets during 0 to 35 days of age, and their response was evaluated in terms of performance, serum biochemical profile, organ weights, liver fat content and bone mineralization. Sunflower oil at 1.5% in diet countered (P ≤ 0.01) the adverse effects of 150 ppb AF on body weight, whereas at 300 ppb AF, such a response was seen at the higher level (3%) of SFO. Aflatoxin decreased (P ≤ 0.01) feed intake by 4 and 11% at 150 and 300 ppb concentration, respectively at 35 days of age, which was increased (P ≤ 0.01) with each incremental level of SFO supplementation (by 3.0 and 8.8%, respectively at 1.5 and 3%, respectively). Serum protein concentration increased (P ≤ 0.01) by SFO supplementation only at the higher concentration (300 ppb) of AF (by 42.4%), whereas total cholesterol and triglyceride concentration, and immune response to SRBC inoculation increased (P ≤ 0.01) with SFO at either level of AF (by 16.8, 18.7 and 75.6% at 1.5% SFO and 33.1, 36.9 and 94.2% at 3.0% SFO, respectively at 35 days of age). Weights of the liver, giblets, kidneys and pancreas increased (P ≤ 0.01) by 23.2, 14.7, 34.2 and 16.9%, respectively, and thymus weight decreased (P ≤ 0.04) by 25.4% with 300 ppb AF, and SFO at 3% in diet countered the effect on weight of the liver and giblets. Fat deposition in the liver increased (P ≤ 0.01) as the concentration of AF increased in diet (by 9.4 and 17.3%, respectively at 150 and 300 ppb AF), which was significantly (P ≤ 0.05) countered by SFO at 3% in diet. Tibia bone Ca content increased by 2.4% (P ≤ 0.01) with SFO supplementation in AF-fed chickens. It is concluded that dietary SFO supplementation countered the adverse effects of AF in broiler chicks in a dose-dependent manner, and higher level of oil (3% in diet) was required at the higher concentration of AF (300 ppb) in diet.

Backyard poultry farming for sustained production and enhanced nutritional and livelihood security with special reference to India: a review.

A comprehensive review on backyard poultry farming (BYPF) was carried out with respect to history, status, production systems, management practices, role in socio-economic development, etc., considering the Indian scenario in particular. Backyard poultry is an age-old traditional practice where small numbers of native chickens are reared with or without inputs under the free-range scavenging conditions. Currently, BYPF contributes about 17.8% (18.41 billion) of the total egg production (103.32 billion) of India. The introduction of high yielding chicken varieties, which resemble the native chicken, transformed the BYPF into a highly remunerative farming activity. The BYPF has a proven potential to alleviate poverty, eradicate malnutrition, empower women, and provide subsidiary income, and gainful employment in rural and tribal areas. In India, four types of backyard poultry farming are practiced, i.e., traditional, small-scale rural, small-scale intensive, and native chicken farming. The aspects of breeding and nutritional strategies in the nursery, and free-range conditions, besides the housing and health care practices that are followed in India, are discussed in detail. Backyard poultry farming has huge potential for further expansion as the produce from this system is preferred across the country.

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India.

Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA genes sequencing (GenBank accession nos. KY412519 and MTCC 25160). Antibiotic susceptibility profile against 20 commercial antibiotics showed its more sensitivity compared to human pathogenic strain, i.e., Klebsiella pneumonia. The compound was identified as phenazine-1-carboxylic acid (PCA) using the multinuclear ID (1H and 13C) and 2D (1H-1H COSY and 1H-13C HETCOR) NMR along with high-resolution mass spectrometry. No significant difference in the bacterial growth in the presence of PCA, FeCl3 and Fe(OH)3 indicated involvement of factors other than PCA in bacterial growth. The study first reports the identification and characterization of PCA from Klebsiella sp. both from terrestrial and marine sources.

Concurrent detection and treatment of cyanide-contaminated water using mechanosynthesized receptors.

The development of receptors that can detect as well as treat cyanide ions in aqueous samples is indispensable for environmental protection. Herein, we present the bulk solvent-free and instant green synthesis of a series of turn-on fluorimetric probes that can specifically detect the deadly poison cyanide among various anions and metal ions in water. Selective recognition of cyanide by the mechanosynthesized compounds is even observable by the naked eyes, which remained unaffected in the presence of various challenging species. NMR spectroscopic investigation supports the chemodosimetric sensing of cyanide by the receptors. A remarkable 55-83 fold fluorescence enhancement by the probes enabled us to reach a limit of detection (LOD) in the range of 8-26 ppb, well below the permissible limit of cyanide in drinking water. Being minuscule soluble in water, cyanide treatment studies with the ionophores showed greater than 99% reduction in the free cyanide concentration after three consecutive cycles of operation. Furthermore, the compounds can be used as sensitive probes for the estimation of cyanide in human blood serum in physiological conditions. Overall, the results presented in this article will certainly find great use in the area of cyanide pollution with regard to simultaneous sensing and treatment of free cyanide, which is heretofore unprecedented.

Evaluation of feeding value of combination of alternate protein sources in White Leghorn layers.

1. A study was conducted to evaluate the effects of including graded levels of an alternate protein mixture (APM) containing cottonseed meal, distillery dried grain with solubles and mustard seed meal in a fixed ratio as a substitute for soybean meal in layer diets (hens aged 27 to 46 weeks). The trial studied the possibility of higher inclusion levels of APM with increased dietary concentrations of crude protein (CP) or critical amino acids (CAA). 2. Maize-soybean meal diet was used as the control diet. In experiment 1, APM was included at 0, 120, 160 and 200 g/kg diet (145 g/kg CP). In experiment 2, the control and three test diets (200 g/kg APM) with three levels of CP (145, 155 and 165 g/kg) were used. In experiment 3, a control and four test diets (200 g/kg APM) with four concentrations of CAA (100%, 105%, 110% and 115% relative to the control) were used. In all experiments, each diet was fed ad libitum to nine replicates of 88 birds each, housed in colony cages. 3. In experiment 1, egg production (EP) and feed intake (FI) were not affected by feeding up to 160 g/kg APM, but, at 200 g/kg, EP was significantly reduced. Feed conversion ratio (FCR) for the hens fed the highest level of APM was similar to the control. Egg weight (EW) and egg mass (EM) in the 160 and 200 g/kg AMS groups were lower than the control group. 4. Increase in dietary CP from 145 to 155 g/kg improved EP and reduced FCR to levels similar to the control group. EM in hens fed 165 g/kg APM was similar to the control in experiment 2. 5. Increasing concentrations of CAA progressively improved EP and reduced FCR in experiment 3. The EP at CAA 110% and FCR at CAA 105% were similar to the control group. 6. Based on the results, it was concluded that the APM can be included up to 160 g/kg diet without affecting layer performance, as long as the diet supplied the recommended levels of protein and amino acids. By increasing either protein from 145 to 155 g/kg or CAA concentrations by 10%, the dietary levels of the APM could be increased to 200 g/kg without affecting the layer performance.

Feeding status of free-range scavenging chickens in different agro-climatic regions of India.

1. The aim of this study was to determine the nutritional status of backyard chickens reared under 4 different agro-climatic conditions (tropical, humid subtropical, cool-temperate and semi-arid) of India during different seasons of the year and to compare their nutritional status with those reared under confined feeding system (CFS). In each season, 15 adult and 15 grower chickens were slaughtered for collecting content of crop and gizzard to assess the nutritional status from different agro-climatic conditions.2. The dry matter content of crop and gizzard was higher (P < 0.01) in chickens reared under backyard conditions compared to those reared under CFS.3. Higher (P < 0.01) crop and gizzard contents were recorded during winter compared to the rainy season. The proportion of grains in the crop during summer and winter season was higher compared to rainy season. Further, there were more insects present in the crop during the rainy season and winter compared to summer.4. The proportion of insects was higher in chickens reared in humid, subtropical and cool temperate regions compared to semi-arid conditions. The consumption of grasses was higher in cool-temperate regions as compared to those reared under other areas.5. Crude protein (CP), Ca, P and gross energy (GE) content of the crop were higher in chickens reared under CFS compared to those reared in backyard systems and CP in the crop contents was lower in backyard chickens reared in humid subtropical and semi-arid regions compared to other areas. Similarly, lower (P < 0.01) Ca levels were seen in crop and gizzard contents from birds reared in semi-arid Rajasthan, and P and GE were lower in tropical wet and dry Telangana (TWT) compared to the chickens reared in the backyard conditions in other regions.6. Based on the results, the nutrient intake for birds utilising the scavenging feed resource base (SFRB) in the TWT region was below the requirements of backyard chickens from the other regions selected in the present study. GE and CP were critically deficient in the SFRB of the TWT region. Therefore, evolving location-specific supplementary diets using locally available feed resources to meet nutrient requirement of backyard chickens is required for optimising production potential.

Effect of feeding diet with alternate protein sources and quality protein maize on performance and nutrient utilization in broiler chickens.

The experiment was conducted to study the effect of feeding diets with quality protein maize (QPM) along with cottonseed meal (CSM) and guar meal (GM) on nutrient utilization, body weight (BW) gain (BWG), feed efficiency, and antioxidant status in broiler chickens. The biological trial was conducted on day-old broiler chicks (n = 240), which were randomly distributed into 6 dietary groups having 8 replicates with 5 chicks. Six experimental diets were formulated to contain maize (diet I), QPM (diet II), maize+CSM80 g/kg + GM40 g/kg (diet III), maize + CSM120 g/kg + GM60 g/kg (diet IV), QPM+CSM80 g/kg + GM40 g/kg (diet V), and QPM+CSM120 g/kg + GM60g/kg (diet VI). The BWG and feed intake were recorded at weekly intervals. Supplementation of CSM and GM along with QPM or maize depressed (P < 0.05) BWG, feed conversion ratio, and slaughter variables in commercial broilers. Improvement of BWG and breast weight was recorded among the groups supplemented 80 g/kg CSM and 40 g/kg GM with a QPM-based diet compared with those groups fed 120 g/kg CSM and 60 g/kg GM with maize or QPM. The energy and protein utilization decreased among the groups supplemented CSM and GM. However, protein and energy utilization was increased (P < 0.05) among the groups fed QPM-based diets compared with those groups fed CSM and GM with maize or QPM. Therefore, it has been concluded that the performance and other parameters did not differ between the groups fed maize- and QPM-based diets in the present experiment. However, QPM with CSM and GM improved the performance, slaughter parameters, and nutrient utilization over CSM and GM with maize.

Estimation of bisulfate in edible plant foods, dog urine, and drugs: picomolar level detection and bio-imaging in living organisms.

In order to explore the properties of any species in solution, the actual, i.e. equilibrium concentration of the free species should be taken into account. Researchers have not paid attention to the deprotonation equilibrium between HSO4- and SO42- while probing bisulfate ion. In this study, we have addressed this concern and developed two zwitterions, CG (coumarin-integrated glycine) and CA (coumarin-integrated alanine), for the selective detection of HSO4- at a picomolar level (50 to 325 pM) with very high binding affinity (∼108 M-1) in pure water at physiological pH. The principle of HSO4- recognition was established via UV-vis and fluorescence techniques. DFT calculations suggested that the H-bonding interactions between the probes and HSO4- are the driving force for this unforeseen selectivity. The membrane penetration ability and nontoxicity of CG/CA enable them to function as staining agents in living brine shrimps and bacteria. The use of these probes for the estimation of HSO4- in various day-to-day edible foods and drugs along with urine samples is unprecedented. The significance and novelty of this study lies in the application and development of assays for estimating bisulfate in several real-world samples that are predominantly aqueous in nature, which are the first of their kind.

Affinity Directed Surface Functionalization of Two Different Metal Nanoparticles by a Natural Ionophore: Probing and Removal of Hg2+ and Al3+ Ions from Aqueous Solutions.

In this work, we report affinity controlled surface modifications of two different metal nanoparticles (MNPs) using a hydrophilic natural ionophore (microbial chelator) aeruginic acid (abbreviated as H2L) that possesses two different types of binding pockets viz. O/O from carboxylic acid group and N/O from a phenol-thiazole moiety. Preferred binding of the former donor set (i.e., O/O) on to the surface of silver nanoparticles (AgNPs) has resulted in a colorimetric nanomaterial HL@AgNPs, which showed naked eye observable easy detection of Hg2+ in aqueous HEPES buffer at pH 7.4, even in the presence of other metal ions. On the other hand, excellent affinity of the phenol-thiazole moiety (i.e., N/O) for iron nanosurfaces (FeNPs) develops a fluorogenic nanomaterial HL@FeNPs. Brilliant emission behavior of this nanomaterial enabled it to be useful for highly selective recognition of Al3+ under identical experimental conditions. Remarkable fluorescence enhancement (122-folds) of HL@FeNPs upon addition of Al3+ remain unchanged even in the presence of other competing metal ions. The nanomaterials HL@AgNPs and HL@FeNPs could even detect the target analytes instantly offering lower detection limits of 2 and 80 nM, respectively. Presence of toxic metal ions as environmental pollutant demands for dual-functional materials capable of performing the task of probing cum removal. Surface functionalizations of the nanomaterials of silver and iron with H2L have also resulted two removal agents that can efficiently and easily extract Hg2+ and Al3+ ions from contaminated water, respectively. We are not aware of any work that highlights the manifold utilization of a microbial chelator (i.e., natural ionophore) in the facile construction of different metal nanoparticles for environmental applications such as detection cum removal of toxic metal ions from aqueous solutions.

Effect of supplementing graded concentrations of non-phytate phosphorus on performance, egg quality and bone mineral variables in White Leghorn layers.

1. An experiment was conducted to determine optimal non-phytate phosphorus (NPP) concentrations for White Leghorn (WL) layers (22-72 weeks) fed diet containing 38 g Ca/kg. 2. Eight diets with graded concentrations (1.5-3.25 g/kg in increments of 0.25 g) of NPP were prepared. Each diet was fed to eight pen replicates containing 88 birds in each. Performance data was evaluated in three different phases (phase I-22-37 weeks, phase II-38-53 weeks and phase III-54-72 weeks). Optimum levels of NPP were determined by fitting a quadratic polynomial (QP) regression model. 3. Egg production (EP) was not affected (P = 0.059) by the concentration of NPP and interaction between NPP and diet phase was non-significant, indicating that the lowest concentration (1.5 g/kg diet) of NPP used in the study was adequate across the three phases. However, EP was influenced by phase (P < 0.001). 4. Optimum concentration of NPP for feed intake (FI) was estimated to be 1.5, 1.71 and 2.40 g/kg diet during phases I, II and III, respectively. FI per egg mass (EM) or feed efficiency (FE) responded quadratically with NPP and also differed significantly between phases. Optimum concentration of NPP for FE during phases I, II and III was 1.5, 2.56 and 2.32 g/kg diet, respectively. 5. Egg weight (EW), EM, shell weight and thickness were not affected by NPP concentration although all of these variables (except shell weight) were influenced by phases. 6. Breaking strength of tibia and Ca contents in tibia ash were not affected by the concentration of NPP, but bone ash and P contents in tibia ash were influenced (P < 0.001) by NPP. Predicted optimal concentrations of NPP for responses for tibia ash at 44 or 72 weeks, tibia ash P at 44 weeks and tibia ash P at 72 weeks were 1.55, 2.63 and 1.5 g/kg diet, respectively. 7. Based on the results, it was concluded that WL layers required 1.5 g, 2.63 g and 2.4 g, respectively/kg diet during phase I, II and III with the calculated daily intake of 137.3, 278.3 and 262 mg NPP/b/d.