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1.
Plant Cell Environ ; 47(5): 1513-1525, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38251425

RESUMO

The DNA damage response avoids mutations into dividing cells. Here, we analysed the role of photoreceptors on the restriction of root growth imposed by genotoxic agents and its relationship with cell viability and performance of meristems. Comparison of root growth of Arabidopsis WT, phyA-211, phyB-9, and phyA-211phyB-9 double mutants unveiled a critical role for phytochrome A (PhyA) in protecting roots from genotoxic stress, regeneration and cell replenishment in the meristematic zone. PhyA was located on primary root tips, where it influences genes related to the repair of DNA, including ERF115 and RAD51. Interestingly, phyA-211 mutants treated with zeocin failed to induce the expression of the repressor of cell cycle MYB3R3, which correlated with expression of the mitotic cyclin CycB1, suggesting that PhyA is required for safeguarding the DNA integrity during cell division. Moreover, the growth of the primary roots of PhyA downstream component HY5 and root growth analyses in darkness suggest that cell viability and DNA damage responses within root meristems may act independently from light and photomorphogenesis. These data support novel roles for PhyA as a key player for stem cell niche maintenance and DNA damage responses, which are critical for proper root growth.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Fitocromo , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Morte Celular , DNA/metabolismo , Reparo do DNA/genética , Luz , Meristema/genética , Meristema/metabolismo , Mutação , Fitocromo/metabolismo , Fitocromo A/genética , Fitocromo A/metabolismo , Fitocromo B/metabolismo
2.
Sensors (Basel) ; 24(2)2024 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-38276372

RESUMO

This study aimed to analyze the effects of contextual variables (i.e., match location and match outcome) and season periods on match load (i.e., internal and external load) in professional Brazilian soccer players. Thirty-six professional players from the same soccer team participated in this study. The season was split into four phases: matches 1-16 (i.e., Phase 1 = P1); matches 17-32 (i.e., Phase 2 = P2); matches 33-48, (i.e., Phase 3 = P3); matches 49-65 (i.e., Phase 4 = P4). Considering match outcome, when the team wins, Cognitive load, Emotional load, and Affective load were significantly higher in away vs. home matches (p < 0.05). Considering season phases, in P3, Mental Fatigue was significantly higher in drawing than in losing matches (p < 0.05). Additionally, considering the match outcome, when the team lost, Total Distance (TD)/min and TD > 19 km·h-1/min were significantly lower in P1 than P2 (p < 0.001), P3 (p < 0.001), and P4 (p < 0.001). These results suggest to strength and conditioning coaches the need to consider the outcome and location of the previous game when planning the week, as well as the phase of the season they are in to reduce fatigue and injury risk.


Assuntos
Desempenho Atlético , Futebol , Humanos , Estações do Ano , Brasil
3.
Biol Sport ; 41(2): 243-248, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38524811

RESUMO

This study aimed to analyse the influence of the match status on the conditional characteristics of tactical sprint actions among Spanish professional soccer players, considering playing positions. Thirty-two Spanish male professional soccer players from a LaLiga Spanish Second Division (LaLiga SmarthBank) team participated in this study. Actions above 85% of the players' maximum velocity were analysed based on their tactical purpose. These findings provide valuable information regarding the tactical aspects of sprinting in soccer, emphasizing the influence of playing positions and match status on the distribution of tactical sprint actions. No effects of match status were observed for any game phase. However, when tactical actions were individually studied, it was observed that the maximum velocity in Chase actions was higher when the team was winning, while in Press actions, the maximum velocity was higher when the team was losing and in in Run in behind/Penetrate, the maximum velocity was higher in drawing situations compared to losing situations. No effects of match status on the distance covered during sprinting were observed, and regarding duration, significant differences were only observed in Recovery run actions. In addition, the influence of match status is higher when playing positions are considered, although the within playing positions analysis revealed significant differences only in CM players. These findings provide valuable information for the design of specific training drills considering playing positions, suggesting the need to analyse the previous match in order to structure the training load of the microcycle in a comprehensive manner.

4.
Planta ; 258(4): 80, 2023 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-37715847

RESUMO

MAIN CONCLUSION: In P. aeruginosa, mutation of the gene encoding N-acyl-L-homoserine lactone synthase LasI drives defense and plant growth promotion, and this latter trait requires adequate nitrate nutrition. Cross-kingdom communication with bacteria is crucial for plant growth and productivity. Here, we show a strong induction of genes for nitrate uptake and assimilation in Arabidopsis seedlings co-cultivated with P. aeruginosa WT (PAO1) or ΔlasI mutants defective on the synthesis of the quorum-sensing signaling molecule N-(3-oxododecanoyl)-L-homoserine lactone. Along with differential induction of defense-related genes, the change from plant growth repression to growth promotion upon bacterial QS disruption, correlated with upregulation of the dual-affinity nitrate transceptor CHL1/AtNRT1/NPF6.3 and the nitrate reductases NIA1 and NIA2. CHL1-GUS was induced in Arabidopsis primary root tips after transfer onto P. aeruginosa ΔlasI streaks at low and high N availability, whereas this bacterium required high concentrations of nitrogen to potentiate root and shoot biomass production and to improve root branching. Arabidopsis chl1-5 and chl1-12 mutants and double mutants in NIA1 and NIA2 nitrate reductases showed compromised growth under low nitrogen availability and failed to mount an effective growth promotion and root branching response even at high NH4NO3. WT P. aeruginosa PAO1 and P. aeruginosa ΔlasI mutant promoted the accumulation of nitric oxide (NO) in roots of both the WT and nia1nia2 double mutants, whereas NO donors SNP or SNAP did not improve growth or root branching in nia1nia2 double mutants with or without bacterial cocultivation. Thus, inoculation of Arabidopsis roots with P. aeruginosa drives gene expression for improved nitrogen acquisition and this macronutrient is critical for the plant growth-promoting effects upon disruption of the LasI quorum-sensing system.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Nitratos , Pseudomonas aeruginosa/genética , Arabidopsis/genética , Lactonas , Acil-Butirolactonas , Nitrato Redutases , Óxido Nítrico , Proteínas de Arabidopsis/genética , Nitrato Redutase/genética
5.
J Sports Sci ; 41(21): 1934-1943, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38258651

RESUMO

This study aimed to assess the effects of a multicomponent training program (ProSAS) on selected measures of physical fitness and joint injuries in Spanish male professional soccer players. Twenty-six professional soccer players (age: 25.2 ± 3.1 years) were randomly assigned to either the experimental (EG; n = 16) or control group (CG; n = 10). Y-balance test (YBT), Single leg countermovement jump (SLCMJ), Single leg hop for distance test (SLH), Side-hop test (SH), Speedy jump test (SpJ), Agility T-test (TT) and Lower extremity functional test (LEFT) were performed before and after an 8-weeks training period. Additionally, any joint injury occurring during the intervention period were recorded. Significant improvements in the EG in all tests (p = 0.001 to p = 0.014), except for SLCMJ (p = 0.632) with the dominant leg were observed. CG displayed decreases in SLCMJ with the non-dominant leg (p = 0.014), SH for both legs (p = 0.001 to p = 0.050), TT (p = 0.005), and LEFT (p = 0.001). Inter-groups differences revealed better results for the EG in all variables (p = 0.001 to p = 0.008) except from SLCMJd (p = 0.555). Moreover, the EG exhibited lower joint injury incidence and burden compared to the CG (p < 0.05). These findings suggest that the ProSAS is an effective multicomponent program to improve variables related to risk of joint injuries, and consequently, to reduce the incidence and burden of these injuries in professional soccer players.


Assuntos
Desempenho Atlético , Futebol , Humanos , Masculino , Adulto Jovem , Adulto , Futebol/lesões , Aptidão Física , Extremidade Inferior , Perna (Membro)
6.
Biol Sport ; 40(3): 723-730, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37398962

RESUMO

The present study presents a novel specific multi-joint isometric test to assess upper limb strength impairment for evidence-based classification in wheelchair sports. Sixteen wheelchair athletes participated in this study and were classified according to their type of physical impairment and health condition as follows: athletes with neurological impairment (ANI, n = 5) and athletes with impaired muscle power (IMP, n = 11). In addition, six non-disabled participants formed a control group (CG, n = 6). All the participants performed the isometric propulsion strength test (IPST), evaluating pushing and pulling actions, and two wheelchair performance tests. Excellent relative intra-session reliability scores were obtained for strength values for the ANI, IMP and CG groups (0.90 < ICC < 0.99) and absolute reproducibility showed acceptable scores of SEM (< 9.52%) for IPST pushing action. The ANI had significantly lower scores in strength and wheelchair performance than the IMP and the CG, while no differences were found between the IMP and the non-disabled participants. In addition, no correlations were found for wheelchair athletes between the isometric upper limb strength measure and wheelchair performance. Our findings suggest that the IPST is a valid test for strength measurement in upper limb impairment wheelchair athletes with different health conditions, which must be used in combination with a performance test to obtain a holistic assessment of this population.

7.
Biol Sport ; 40(1): 111-147, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36636192

RESUMO

One of the most often-used task constraints in designing small-sided games (SSGs) is the manipulation of pitch size to promote increases or decreases in the relative area per player. Such adjustments cause changes in the acute responses during SSGs. This systematic review with meta-analysis aimed to compare the effects of smaller vs. larger pitch sizes on soccer players' physiological, physical, technical, and tactical responses during SSGs. Comparisons between smaller and larger pitches were not considered based on a specific size, but also between using at least two dimensions in the same comparative study, aiming to understand differences between using smaller and larger (independently of the specific dimensions). The data sources utilized were PubMed, PsycINFO, Scielo, Scopus, SPORTDiscus, and Web of Science. The database search initially yielded 249 titles. From those, 41 articles were eligible for the systematic review and meta-analysis. Results revealed that, compared to smaller pitches, SSGs played on larger pitches induced greater values for heart rate (p < 0.001; ES = 0.50), rate of perceived exertion (p < 0.001; ES = 0.70), total distance (p < 0.001; ES = 1.95), high-speed running (p < 0.001; ES = 1.20), stretch index (p < 0.001; ES = 1.02) and surface area (p < 0.001; ES = 1.54). No significant differences were found between pitch size regarding the numbers of accelerations (p = 0.232; ES = 0.45), decelerations (p = 0.111; ES = 0.85), passes (p = 0.897; ES = 0.02), dribbles (p = 0.823; ES = -0.05), or positional centroid (p = 0.053; ES = 0.56). Larger pitch sizes can be implemented as a meaningful task constraint to increase the internal and external load experienced by soccer players during SSGs, as well as to increase the dispersion of players while acting together. These results were found independent of format and age group.

8.
Res Sports Med ; 31(5): 588-603, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-34841995

RESUMO

This study analysed the effects of a training program based on Nordic hamstring and sprint exercises on physical performance and hamstring injuries in young male soccer players. Forty-nine U19 players were randomly assigned to a control (CG; n = 26) or experimental group (EG; n = 23). Linear sprint and with change of direction (COD) were assessed before and after a 14-week training period. Hamstring injuries were collected during the intervention period. Between-groups analysis revealed differences in linear sprint performance (p = 0.012-0.001) in favour of the EG. Pre-to-post performance increased significantly in the EG for 20 m (effect size [ES] = -0.56) and 30 m (ES = -0.62) sprints, but a significant reduction in some COD parameters was observed (ES = 0.45-0.57). In CG, only a significant reduction in COD with dominant leg was found (ES = 0.63). Significant differences in injury burden in favour of the EG was reported such as (27.87 [CG] vs. 3.82 [EG] absence days/1000 h of exposure, rate ratio = 7.30, 95% CI 3.34-15.99). While injury incidence was not different between the EG and CG. These findings suggest that the training program implemented can improve sprint performance and reduce injury burden.

9.
Res Sports Med ; 31(5): 574-587, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-34852693

RESUMO

This study examined the effects of body mass-based resistance training (bmRT) on selected measures of physical fitness and injury incidence and burden in soccer players. Forty-six U16 male soccer players were randomly assigned to an control (CG; n = 26) or experimental group (EG; n = 20) Countermovement jump (CMJ), change of direction (CoD) (i.e., 20 m with one CoD), and linear sprint over 30 m were assessed before and after a 15-weeks training. Any type of musculotendinous injury that occurred throughout the intervention period was recorded. Between-group difference was noted at post-test for CMJ (p = 0.008). Pre-to-post training values increased in the EG (effect size [ES] = 1.01) while in the CG no pre-to-post changes were detected (ES = 0.27). No between-group differences at post-test were observed for sprint and CoD tests. Differences in injury burden were reported (33.28 [CG] vs. 9.55 [EG] absence days/1,000 hours exposure, rate ratio = 3.49, 95% CI 2.03-6.00, p < 0.001), but not in injury incidence. A bmRT programis suitable for improving jumping height but not linear and CoD speed performance. Additionally, bmRT may reduce injury burden, and therefore, the severity of musculotendinous injuries in U16 male soccer players.

10.
Res Sports Med ; 31(3): 228-248, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-34315310

RESUMO

The aims of the current systematic review were to evaluate the current literature surrounding the chronic effect of flywheel training on the physical capacities of soccer players, and to identify areas for future research to establish guidelines for its use.Studies were identified following a search of electronic databases (PubMed and SPORTDiscus) in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols (PRISMA).Eleven studies met the inclusion criteria and were included. The methodological quality of the included studies ranged between 10 and 18 with an average score of 15 points using the PEDro scale. The training duration ranged from 6 weeks to 27 weeks, with volume ranging from 1 to 6 sets and 6 to 10 repetitions, and frequency from 1 to 2 times a week. This systematic review reported that a diverse range of flywheel training interventions can effectively improve strength, power, jump, and changes of direction in male soccer players of varying levels.Flywheel training interventions improve the physical capacities of soccer players of varying levels. Nonetheless, the current literature suggests contrasting evidence regarding flywheel training induced changes in sprint speed and acceleration capacity of soccer players.


Assuntos
Desempenho Atlético , Futebol , Humanos , Masculino , Força Muscular , Aceleração
11.
J Strength Cond Res ; 36(4): 888-894, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32412970

RESUMO

ABSTRACT: Sanchez-Sanchez, J, Raya-González, J, Ramirez-Campillo, R, Chaabene, H, Petisco, C, and Nakamura, FY. The increased effectiveness of resistance training on unstable vs. stable surfaces on selected measures of physical performance in young male soccer players. J Strength Cond Res 36(4): 888-894, 2022-To examine the effects of 10-week (2/wk) resistance training on stable vs. unstable surfaces on selected measures of physical performance in young male soccer players, national-level U19 players participated in this study. They were randomly allocated to an unstable resistance training group (uRT, n = 27) or a stable resistance training group (sRT, n = 28). Before and after the training, horizontal jumping with dominant (Hop D) and nondominant leg (Hop non-D), repeated sprint ability (RSA best time [RSAbest] and RSA mean time [RSAmean]), change-of-direction (COD) speed (Illinois COD test), and aerobic endurance (YoYo Intermittent Recovery Test Level 1 [YoYo IR1]) were assessed. To establish the effects of the interventions on the dependent variables, a 2 (group: uRT and sRT) × 2 (time: pre, post) analysis of variance with repeated measures on time was computed. A significant main effect of time was observed for Hop non-D, RSAbest, and RSAmean (p = 0.003-0.06, effect size [ES] = 0.06-0.15). Furthermore, significant group × time interactions were shown for RSAbest (p = 0.007, ES = 0.13) and RSAmean (p = 0.002, ES = 0.2). Post hoc analysis revealed significant pre- to post-training improvements for RSAbest (p = 0.002, ES = 0.35) and RSAmean (p = 0.0002, ES = 0.36) in the uRT. In the sRT, however, no significant pre-post performance changes were observed in RSAbest and RSAmean. In conclusion, 10 weeks of an in-season resistance training on unstable conditions in addition to regular soccer training was effective in improving repeated-sprint ability performance in youth male elite soccer players including maximal linear sprinting and the ability to perform repeated sprint.


Assuntos
Desempenho Atlético , Treinamento Resistido , Corrida , Futebol , Adolescente , Humanos , Masculino , Desempenho Físico Funcional
12.
Biol Sport ; 39(4): 809-817, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36247955

RESUMO

Growing evidence supports use of eccentric methods for strength development and injury prevention within elite soccer, yet uncertainty remains regarding practitioners' application of flywheel (isoinertial) methods. The aims of this study were to investigate how the flywheel training literature is perceived and applied by elite soccer practitioners, highlight gaps in knowledge and develop industry-relevant research questions. Fifty-one practitioners completed an electronic questionnaire. Fourteen Likert scale statements were grouped into topics: strength and performance; post-activation performance enhancement and methodological considerations; chronic strength; chronic performance; injury prevention. Three general questions followed, allowing more detail about flywheel training application. A Majority of the participants reported ≥ 2 years' experience of programming flywheel training. Nearly all participants agreed that familiarisation is needed. Practitioners agree that flywheel training can improve sport performance, strength and likelihood of non-contact injury outcomes. Most practitioners prescribe 2 weekly sessions during pre- and in-season periods. Flywheel sessions mostly consist of squats but a variety of exercises (lunge, hip hinge, and open kinetic chain) are also frequently included. Practitioners are mostly unsure about differences between flywheel and traditional resistance training equipment and outcomes, practicality of flywheel equipment, and evidence-based guidelines. The investigation provides valuable insight into the perspectives and application of flywheel training within elite soccer, highlighting its perceived efficacy for strength and injury prevention.

13.
Biol Sport ; 39(4): 1081-1086, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36247957

RESUMO

The main objective of this study was to analyse the changes in external demand parameters (e.g., total distance, high-speed running distance, accelerations/decelerations) in Spanish professional soccer teams after the COVID-19 lockdown considering their on-field ranking (i.e., teams whose ranking worsened after the COVID-19 lockdown [WRS] vs. teams that improved their ranking after the COVID-19 lockdown [IMP]). A total of 23,527 individual match observations were collected on players competing during the 2019/20 season in the First Spanish Professional soccer League (LaLiga). Goalkeepers and players who participated for less than 10 minutes in each match were excluded. Relative total distance (TD/min), distance covered at 21-24 km · h-1 (HIRD/min) and > 24 km · h-1 per minute (VHIRD/min), high metabolic load distance (HMLD), and the number of accelerations (3 m/s2) and decelerations (< 3 m/s2) performed were analysed by the ChryonHego video-tracking system. These variables were analysed during two differentiated periods, before the COVID-19 lockdown (i.e., 27 matches) and after the COVID-19 lockdown (i.e., 11 matches), and teams were classified into two groups according to their ranking (i.e., WRS vs. IMP). R-Studio was employed for data analysis and a mixed linear model was conducted. A decrease in external demands in all teams after the COVID-19 lockdown was observed, and this decrease was greater in WRS. These results suggest that, after an inactive period (i.e., the COVID-19 lockdown), teams that return with better physical performance, mainly related to high-intensity actions, have more possibilities of improving their final qualifying position.

14.
Biol Sport ; 39(3): 765-778, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35959331

RESUMO

Plyometric jump training (PJT) can be used for improving balance through bilateral and unilateral jump-landing drills. Since the increased number of articles testing the effects of PJT on dynamic and static balance, it is relevant to summarize the evidence and determine the effects across different original articles. This systematic review with meta-analysis was conducted to assess the effects of PJT programs on dynamic and static balance in soccer players. The data sources utilized were Cochrane, Medline (PubMed), SPORTDiscus, and Web of Science. (i) Soccer players of any age or sex without injury, illness, or other clinical conditions; (ii) PJT-based programs restricted to a minimum of three weeks (duration); (iii) passive or active control groups; (iv) pre-post interventions values of dynamic and/or static balance; (v) randomized-controlled trials; and (vi) peerreviewed original full-text studies written in English, Portuguese, and/or Spanish. The database search initially identified 803 titles. From those, eight articles were eligible for the systematic review and meta-analysis. The results showed no significant differences between PJT and active controls in dynamic anterior, postero-medial, or postero-lateral balance for both left and right legs (p > 0.05). Additionally, no significant differences were found between PJT and active controls in terms of static balance (p = 0.495). The current evidence suggests that PJT has no significant advantage over active control groups in terms of dynamic or static balance.

15.
Res Sports Med ; 30(4): 353-370, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-33401963

RESUMO

This study aimed to evaluate the effect of flywheel training on female populations, report practical recommendations for practitioners based on the currently available evidence, underline the limitations of current literature, and establish future research directions. Studies were searched through the electronic databases (PubMed, SPORTDiscus, and Web of Science) following the preferred reporting items for systematic reviews and meta-analysis statement guidelines. The methodological quality of the seven studies included in this review ranged from 10 to 19 points (good to excellent), with an average score of 14-points (good). These studies were carried out between 2004 and 2019 and comprised a total of 100 female participants. The training duration ranged from 5 weeks to 24 weeks, with volume ranging from 1 to 4 sets and 7 to 12 repetitions, and frequency ranged from 1 to 3 times a week. The contemporary literature suggests that flywheel training is a safe and time-effective strategy to enhance physical outcomes with young and elderly females. With this information, practitioners may be inclined to prescribe flywheel training as an effective countermeasure for injuries or falls and as potent stimulus for physical enhancement.


Assuntos
Força Muscular , Idoso , Feminino , Humanos
16.
Res Sports Med ; : 1-15, 2022 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-35616245

RESUMO

This study aimed to analyse the effects of a 16-week jump training program on the physical performance and lower extremities injury profile in semi-professional male soccer players. Participants were randomly assigned to the control group (CG; n = 13; age = 21.7 ± 3.6 years) or the experimental group (EG; n = 10; age = 22.3 ± 3.5 years). Countermovement jump (CMJ) height (cm), 30 m linear sprint time (s) with split times at 10 m and 20 m distances, and change of direction speed (CODS; 10 + 10 m with 90° turn) time (s) with turns using the dominant or non-dominant leg, were assessed before and after the intervention. Lower extremity injuries sustained throughout the intervention period were collected. Significant within-group improvements were found in EG in CMJ (p = 0.01; effect size [ES] = 1.03; large). Additionally, between-group difference after intervention was found in CMJ (F = 4.42; p = 0.013) in favour of EG. Injury burden was 194.86 (CG) vs 71.37 (EG) days of absence/1,000 h (RR = 2.73; 95% CI 2.10-3.54; p < 0.001). No other significant within-group or between-group differences were found. In conclusion, compared to regular soccer training, jump training was effective to improve jumping ability and burden in soccer players.

17.
New Phytol ; 229(3): 1278-1288, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33034045

RESUMO

Phosphate (Pi ) is a critical macronutrient for the biochemical and molecular functions of cells. Under phosphate limitation, plants manifest adaptative strategies to increase phosphate scavenging. However, how low phosphate sensing links to the transcriptional machinery remains unknown. The role of the MEDIATOR (MED) transcriptional co-activator, through its MED16 subunit in Arabidopsis root system architecture remodeling in response to phosphate limitation was assessed. Its critical function acting over the SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1)-ALUMINUM-ACTIVATED MALATE TRANSPORT1 (ALMT1) signaling module was tested through a combination of genetic, biochemical, and genome-wide transcriptomic approaches. Root system configuration in response to phosphate scarcity involved MED16 functioning, which modulates the expression of a large set of low-phosphate-induced genes that respond to local and systemic signals in the Arabidopsis root tip, including those directly activated by STOP1. Biomolecular fluorescence complementation analysis suggests that MED16 is required for the transcriptional activation of STOP1 targets, including the membrane permease ALMT1, to increase malate exudation in response to low phosphate. Our results unveil the function of a critical transcriptional component, MED16, in the root adaptive responses to a scarce plant macronutrient, which helps understanding how plant cells orchestrate root morphogenesis to gene expression with the STOP1-ALMT1 module.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Fosfatos/metabolismo , Raízes de Plantas/metabolismo , Transativadores , Fatores de Transcrição/genética
18.
J Strength Cond Res ; 35(6): 1593-1598, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32398632

RESUMO

ABSTRACT: Raya-González, J, García-Esteban, S, Hume, P, and Castillo, D. Effects of gluteal muscles strengthening on lower-limb injuries in male professional handball players: a preliminary study. J Strength Cond Res 35(6): 1593-1598, 2021-This study aimed to analyze the effects of a strength training injury-prevention program (STIPP) on injuries associated with weakness of the gluteal muscles in professional handball players. Twenty-seven professional Spanish male handball players from the same club, who competed at the Spanish Second National League level, participated in this study. The investigation was conducted over 2 consecutive seasons (2017-2018 and 2018-2019). The first season served as the control season (n = 21), and the second season served as the experimental season (n = 20). The STIPP was conducted 2 times per week, for 32 weeks, during the in-season period. There were no significant improvements in the injury incidence, pattern, or type for the experimental season group compared with the control season group (injury rate ratio [IRR] = 1.47; 95% confidence interval [CI]: 0.84-2.58; p = 0.17). However, there was a meaningful reduction in the number and burden of lumbar injuries after the STIPP (IRR = 55.83; 95% CI: 0.11-89.01; p = 0.001). In addition, burden values (number of absence days/1000 hours exposure) in all injury pathologies were lower during the experimental season compared with the control season. The STIPP focused on the gluteal muscles could be effective in reducing the number and burden of lumbar injuries in professional handball players.


Assuntos
Traumatismos em Atletas , Treinamento Resistido , Esportes , Traumatismos em Atletas/prevenção & controle , Humanos , Incidência , Extremidade Inferior , Masculino , Músculos
19.
J Strength Cond Res ; 35(12): 3453-3458, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31469765

RESUMO

ABSTRACT: Castillo, D, Raya-González, J, Weston, M, and Yanci, J. Distribution of external load during acquisition training sessions and match play of a professional soccer team. J Strength Cond Res 35(12): 3453-3458, 2021-The aim of this study was to use global position systems to analyze the external loads of the 3 different acquisition training sessions (ATS) with competitive matches in professional soccer players over a 6-week period. Sixteen professional soccer players participated in the study, which analyzed the distribution of external load during the training microcycle of a professional soccer team. The 3 types of ATS undertaken by the players were as follows: ATS1 (strength), ATS2 (endurance), and ATS3 (speed). The total distance covered, the distance covered at above 14 km·h-1, the distance covered >21 km·h-1, the number of high accelerations (>3 m·s-2), and player load were recorded. The results showed that external loads were consistently higher during matches when compared with all training sessions (range of effect sizes: 1.06-3.38). Between training session comparisons revealed higher external loads during ATS1 and ATS2, when compared with ATS3 (range of effect sizes: 0.60-2.41). The only external load variable that differed between ATS1 and ATS2 was the distance covered >21 km·h-1, which was higher for ATS1. Our findings suggest that technical staff should consider the physical demands of weekly periodization to understand the training process regarding optimizing player physical performance.


Assuntos
Desempenho Atlético , Futebol , Aceleração , Humanos , Estado Nutricional , Exame Físico
20.
J Strength Cond Res ; 35(8): 2287-2293, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-30908372

RESUMO

ABSTRACT: Castillo, D, Rodríguez-Fernández, A, Nakamura, FY, Sanchez-Sanchez, J, Ramirez-Campillo, R, Yanci, J, Zubillaga, A, and Raya-González, J. Influence of different small-sided game formats on physical and physiological demands and physical performance in young soccer players. J Strength Cond Res 35(8): 2287-2293, 2021-The aim of this study was to quantify the acute impact of bout duration and individual interaction space on physical and physiological demands and on physical performance. Ten young male soccer players (age: 14.8 ± 0.6 years) from the same team playing in the National U-16 Division participated. Physical (total distance [TD]; distance covered at different speeds; and maximum velocity [Velmax]) and physiological (peak [HRpeak] and mean [HRmean] heart rate) parameters were collected for every bout during each small-sided game (SSG) format. Moreover, the effects of SSGs on horizontal jump (HJ) and 30-m sprint performances were evaluated. The SSG formats were composed of 6 players a side (including goalkeepers) and included 4 repetitions of 6 minutes in a space of 100 m2 (SSG1) or 200 m2 (SSG2) and 6 repetitions of 4 minutes in 100 m2 (SSG3) or 200 m2 (SSG4). The TD, the distance covered at different speeds, and Velmax were greater (p < 0.01, effect size [ES] = 1.25-5.95, large) in SSG2 and SSG4 than SSG1 and SSG3, respectively. Furthermore, the HRmean and HRpeak were lower (p < 0.05, ES = 1.53-2.23, large) during SSG3 than other SSGs. In addition, while a significant (p < 0.05, ES = 0.70-2.04, moderate to large) increase in SPR30 time in SSG1 and SSG3 was observed, HJ performance was not affected (p > 0.05, ES = 0.03-0.54, trivial to moderate) by any SSG format. These findings suggest increasing pitch size to induce greater physical demands and to use SSGs with smaller pitch size, and independently of the bout duration, to induce neuromuscular fatigue.


Assuntos
Desempenho Atlético , Corrida , Futebol , Adolescente , Frequência Cardíaca , Humanos , Masculino , Desempenho Físico Funcional
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