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1.
Eur Radiol ; 33(2): 1456-1464, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35976397

RESUMO

OBJECTIVE: To evaluate anterolateral ligament abnormalities (ALL) at MRI scans and correlate with data from clinical instability tests (Lachman and pivot shift) performed under anesthesia in patients with acute anterior cruciate ligament (ACL) tears. Furthermore, perform multivariate analysis with other variables to isolate the ALL contribution to instability from other abnormalities. METHODS: Retrospective analysis of MRI and instability tests of 95 patients with ACL tears. The ALL was classified as no abnormality, abnormality without discontinuity, and discontinuity. Injuries in other knee ligament structures and menisci were also assessed. Results of instability tests (pivot shift and Lachman) with the patient anesthetized for arthroscopic ACL reconstruction were obtained from the patient database. Statistical analysis was performed using the IBM SPSS 22. RESULTS: ALL abnormalities and iliotibial band (ITB), lateral (LCL), and medial (MCL) collateral ligament injuries showed a statistically significant correlation with the pivot shift test. The ALL MRI abnormalities were associated with the high-grade pivot shift results (p < 0.0005), with an odds ratio of 55.9 for high degrees of pivot shift in patients with abnormal ALL. The logistic model for all variables analyzed with the results of the pivot shift test demonstrated that the ALL was the only variable with a statistically significant correlation in the model (p < 0.0005). CONCLUSION: MRI ALL abnormalities in patients with ACL injuries have a significant association with pivot shift test results in patients under anesthesia. The logistic model for high - grade pivot shift test results demonstrated that ALL abnormalities were the only variable with significant correlation. KEY POINTS: • Evaluating the anterolateral ligament with MRI in acute anterior cruciate ligament injuries is useful to predict higher grades of pivot shift test in the moment of the arthroscopic reconstruction. • An abnormal anterolateral ligament presented an odds ratio of 55 for high degrees of pivot shift. • ALL MRI abnormalities association with knee instability is independent from other internal knee injuries.


Assuntos
Lesões do Ligamento Cruzado Anterior , Instabilidade Articular , Humanos , Lesões do Ligamento Cruzado Anterior/complicações , Lesões do Ligamento Cruzado Anterior/diagnóstico por imagem , Lesões do Ligamento Cruzado Anterior/cirurgia , Estudos Retrospectivos , Articulação do Joelho/diagnóstico por imagem , Articulação do Joelho/cirurgia , Ligamentos Articulares/diagnóstico por imagem , Instabilidade Articular/diagnóstico por imagem , Instabilidade Articular/cirurgia , Imageamento por Ressonância Magnética
2.
Org Biomol Chem ; 21(22): 4606-4619, 2023 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-37042164

RESUMO

In this work, we describe the design, synthesis, characterization, photophysical evaluation, DFT calculations, and application of two novel fluorescent benzothiadiazole (BTD) sensors for hydrazine detection and quantification at the cellular and multicellular (in vivo) levels. The two probes were fully characterized, and their photophysical properties were evaluated. We tested the designed fluorogenic dye (named BTD-CHO) as a selective sensor for the rapid, sensitive, and selective detection of hydrazine. When treated with N2H4, the probe affords a new derivative named BTD-HZN, releasing water as the only byproduct. BTD-CHO exhibited a preference for lipid droplets (LDs) and accumulated inside these organelles. Hydrazine detection in LDs could be carried out by the in situ formation of BTD-HZN inside live cells. We efficiently visualized the lipids of a challenging cellular model, microalgae (Chlorella sorokiniana), using these sensors. In vivo experiments indicated rapid and efficient detection of the analyte using C. elegans and zebrafish (Danio rerio) as the multicellular models.


Assuntos
Chlorella , Corantes Fluorescentes , Animais , Gotículas Lipídicas , Peixe-Zebra , Caenorhabditis elegans , Hidrazinas
3.
Skeletal Radiol ; 52(7): 1263-1276, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-36534142

RESUMO

Clinical signs and symptoms of venous thrombosis and musculoskeletal pathologies frequently overlap. Sometimes, patients with venous thrombosis undergo MR examinations under an equivocal suspicion of muscle, tendon or articular injury. A low pretest clinical suspicion and lack of familiarity with the conventional MR imaging signs of venous thrombosis may result in failure to diagnose venous thrombosis, delaying treatment and raising morbimortality. In MR imaging, thrombosis presents as venous ectasia with intraluminal heterogeneous content. Small vein thrombosis is often identified as having a branching aspect. Perivenous edema and inflammatory soft tissue changes may be the most prominent findings. The purpose of this paper is to illustrate MR findings of venous thrombosis in patients who underwent MR examinations due to suspected musculoskeletal pathologies. Cases of venous thrombosis in different sites of the body diagnosed through MR are presented.


Assuntos
Imageamento por Ressonância Magnética , Trombose Venosa , Humanos , Trombose Venosa/diagnóstico por imagem
4.
Skeletal Radiol ; 51(6): 1127-1141, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34693455

RESUMO

The term progressive collapsing foot deformity (PCFD) is currently recommended as the replacement to adult-acquired flatfoot deformity and posterior tibial tendon dysfunction to better reflect its pathology, which consists of a complex three-dimensional deformity involving the foot and ankle. The new consensus has also provided a new classification that requires clinical and radiographic findings for patient stratification into each class. However, conventional radiographs are susceptible to errors resulting from the inadequate positioning of patients, incorrect angulation of the X-ray tube, and overlapping of bone structures. Weightbearing cone beam computed tomography (WBCBCT), which has greater diagnostic accuracy than conventional radiograph, is useful for evaluating progressive collapsing foot deformity to determine medial arch collapse, hindfoot alignment, peritalar subluxation, posterior subtalar joint valgus, intrinsic talus valgus, and lateral extra-articular bone impingement. The present review aimed to discuss the new recommendations for nomenclature, classification, and imaging evaluation of PCFD, with an illustrative and quantitative focus on the measurements used in conventional radiography and WBCBCT. The measurements presented here are important criteria for decision-making.


Assuntos
Pé Chato , Deformidades do Pé , Articulação Talocalcânea , Adulto , Tomografia Computadorizada de Feixe Cônico , Pé Chato/diagnóstico por imagem , Humanos , Suporte de Carga
5.
Sensors (Basel) ; 22(9)2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35590893

RESUMO

This work presents an experimental study related to the mechanical performance of a special design spring fabricated with a superelastic shape memory alloy (SMA-SE). For the experimental testing, the spring was coupled in a rotor machine, aiming to attenuate the mechanical vibration when the system went through a natural frequency without any external power source. It was verified that the reduction in instabilities stemmed from the better distribution of vibration force in the proposed device, as well as the damping capacity of the spring material. These findings showed that the application of the M-Shape device of SMA-SE for three different cases could reduce vibration up to 23 dB when compared to the situations without, and with, 1.5 mm of preload. The M-Shape device was shown to be efficient in reducing the mechanical vibration in a rotor system. This was due to the damping capacity of the SMA-SE material, and because the application did not require any external source of energy to generate phase transformation.

6.
J Exp Bot ; 72(5): 1864-1878, 2021 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-33211856

RESUMO

The phenotype of an individual emerges from the interaction of its genotype with the environment in which it is located. Phenotypic plasticity (PP) is the ability of a specific genotype to present multiple phenotypes in response to the environment. Past and current methods for quantification of PP present limitations, mainly in what constitutes a systemic analysis of multiple traits. This research proposes an integrative index for quantifying and evaluating PP. The multivariate plasticity index (MVPi) was calculated based on the Euclidian distance between scores of a canonical variate analysis. It was evaluated for leaf physiological traits in two cases using Brazilian Cerrado species and sugarcane varieties, grown under diverse environmental conditions. The MVPi was sensitive to plant behaviour from simple to complex genotype-environment interactions and was able to inform coarse and fine changes in PP. It was correlated to biomass allocation, showing agreement between plant organizational levels. The new method proved to be elucidative of plant metabolic changes, mainly by explaining PP as an integrated process and emergent property. We recommend the MVPi method as a tool for analysis of phenotypic plasticity in the context of a systemic evaluation of plant phenotypic traits.


Assuntos
Adaptação Fisiológica , Folhas de Planta , Brasil , Genótipo , Fenótipo
7.
Chem Rec ; 21(10): 2762-2781, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33538117

RESUMO

We review the most innovative efforts and greatest challenges faced when elucidating multicomponent reactions (MCRs) mechanisms. When compared to traditional reactions, the often two or more concurrent reactions pathways and the greater number of possible intermediates in MCRs turn their mechanistic investigation both a harder and trickier task. The common approaches used to investigate reaction mechanisms are often unable to clarify MCRs mechanisms; hence few but clever approaches are currently used to determine these mechanisms and to depict their key transformations. Their complexity has required most innovative approaches and the use of a number of unique techniques that have shed light over the favored pathway selected from the myriad of alternatives theoretically available for MCRs. This review focuses on the most successful efforts applied by a few leading groups to perform these puzzlingly investigations.

8.
Semin Musculoskelet Radiol ; 25(4): 580-588, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34706388

RESUMO

Ulnar collateral, radial collateral, lateral ulnar collateral, and annular ligaments can be injured in an acute trauma, such as valgus stress in athletes and elbow dislocation. Recognizing normal anatomy in magnetic resonance imaging and ultrasonography studies is important to identify ligamentous abnormalities in these imaging modalities.


Assuntos
Ligamentos Colaterais , Articulação do Cotovelo , Luxações Articulares , Ligamentos Colaterais/diagnóstico por imagem , Cotovelo/diagnóstico por imagem , Articulação do Cotovelo/diagnóstico por imagem , Humanos , Ulna
9.
Arthroscopy ; 37(11): 3307-3315, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-33940130

RESUMO

PURPOSE: To report the arthroscopic treatment results of a degenerative medial meniscus tear with a displaced flap into the meniscotibial recess, tibial peripheral reactive bone edema, and focal knee medial pain. As a secondary objective, we propose to identify possible factors associated with a good or poor prognosis of the surgical treatment of this lesion. METHODS: From 2012 to 2018, patients who had this specific meniscus pathology and underwent arthroscopic surgical treatment were retrospectively evaluated. Patients with Kellgren-Lawrence (KL) classification greater than 2 were excluded. KL classification, the presence of an Outerbridge grade III/V chondral lesion of the medial compartment, limb alignment, body mass index, and smoking were evaluated. The subjective outcomes included the International Knee Documentation Committee score, improvement in the pain reported by patients, and the Global Perceived Effect (GPE) scale score. RESULTS: A total of 69 patients were evaluated. The mean age was 58.6 ± 7.1 years. The follow-up time was 48.7 ± 20.8 months. Fifty-five (79.7%) patients reported pain improvement. The postoperative International Knee Documentation Committee was 62.6 ± 15.4, and the mean GPE was 2.3 ± 2.6. Fourteen patients (20.3%) showed no improvement in pain, and 7 patients (10.2%) presented complications. Groups that improved (GPE > 0) and did not improve (GPE < 0) did not present differences regarding age, sex, follow-up time, chondral lesions, or body mass index. Patients without improvement had a greater incidence of smoking (P = .001), varus alignment (P = .008), and more advanced KL classification (P < .001). In the multivariate analysis based on the GPE score, KL classification (P = .038) and smoking (P = .003) were significant. CONCLUSIONS: Arthroscopic surgical treatment of degenerative medial meniscal tears with a meniscal flap displaced into the meniscotibial recess and adjacent focal bone edema in the tibia shows good results in approximately 80% of cases. Smoking and KL grade 2 were factors associated with poor prognosis of surgical treatment. LEVEL OF EVIDENCE: Level IV (case series).


Assuntos
Meniscos Tibiais , Lesões do Menisco Tibial , Idoso , Artroscopia , Edema/etiologia , Humanos , Meniscos Tibiais/cirurgia , Pessoa de Meia-Idade , Estudos Retrospectivos , Tíbia/cirurgia , Lesões do Menisco Tibial/cirurgia
10.
Sensors (Basel) ; 21(20)2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34696138

RESUMO

The classic monitoring methods for detecting faults in automotive vehicles based on on-board diagnostics (OBD) are insufficient when diagnosing several mechanical failures. Other sensing techniques present drawbacks such as high invasiveness and limited physical range. The present work presents a fully noninvasive system for fault detection and isolation in internal combustion engines through sound signals processing. An acquisition system was developed, whose data are transmitted to a smartphone in which the signal is processed, and the user has access to the information. A study of the chaotic behavior of the vehicle was carried out, and the feasibility of using fractal dimensions as a tool to diagnose engine misfire and problems in the alternator belt was verified. An artificial neural network was used for fault classification using the fractal dimension data extracted from the sound of the engine. For comparison purposes, a strategy based on wavelet multiresolution analysis was also implemented. The proposed solution allows a diagnosis without having any contact with the vehicle, with low computational cost, without the need for installing sensors, and in real time. The system and method were validated through experimental tests, with a success rate of 99% for the faults under consideration.


Assuntos
Algoritmos , Redes Neurais de Computação , Processamento de Sinais Assistido por Computador , Análise de Ondaletas
11.
Molecules ; 27(1)2021 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-35011363

RESUMO

In this review, we comprehensively describe catalyzed multicomponent reactions (MCRs) and the multiple roles of catalysis combined with key parameters to perform these transformations. Besides improving yields and shortening reaction times, catalysis is vital to achieving greener protocols and to furthering the MCR field of research. Considering that MCRs typically have two or more possible reaction pathways to explain the transformation, catalysis is essential for selecting a reaction route and avoiding byproduct formation. Key parameters, such as temperature, catalyst amounts and reagent quantities, were analyzed. Solvent effects, which are likely the most neglected topic in MCRs, as well as their combined roles with catalysis, are critically discussed. Stereocontrolled MCRs, rarely observed without the presence of a catalytic system, are also presented and discussed in this review. Perspectives on the use of catalytic systems for improved and greener MCRs are finally presented.

12.
Haemophilia ; 26(4): 565-574, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32497355

RESUMO

A goal of the International Prophylaxis Study Group (IPSG) is to provide an accurate instrument to measure MRI-based disease severity of haemophilic arthropathy at various time points, so that longitudinal changes in disease severity can be identified to support decisions on treatment management. We review and discuss in this paper the evaluative purpose of the IPSG MRI scale in relation to its development and validation processes so far. We also critically appraise the validity, reliability and responsiveness of using the IPSG MRI scale in different clinical and research settings, and whenever applicable, compare these clinimetric properties of the IPSG MRI scale with those of its precursors, the compatible additive and progressive MRI scales.


Assuntos
Hemartrose/diagnóstico , Hemartrose/prevenção & controle , Artropatias/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Adolescente , Articulação do Tornozelo/diagnóstico por imagem , Articulação do Tornozelo/patologia , Criança , Pré-Escolar , Hemartrose/etiologia , Hemartrose/patologia , Hemofilia A/complicações , Hemofilia A/terapia , Hemossiderina/análise , Humanos , Artropatias/etiologia , Articulação do Joelho/diagnóstico por imagem , Articulação do Joelho/patologia , Masculino , Variações Dependentes do Observador , Reprodutibilidade dos Testes , Índice de Gravidade de Doença , Membrana Sinovial/metabolismo , Membrana Sinovial/patologia
13.
BMC Infect Dis ; 20(1): 114, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32041537

RESUMO

BACKGROUND: Identifying patients with hepatitis C virus (HCV) infection and enhancing the cascade of care are essential for eliminating HCV infection. This study aimed to estimate the prevalence of positive anti-HCV serology in Brasilia, Brazil, and evaluate the efficiency of the cascade of care for HCV-positive individuals. METHODS: This cross-sectional study analyzed 57,697 rapid screening tests for hepatitis C in individuals aged > 40 years between June 2018 and June 2019. HCV-positive patients were contacted and scheduled to undergo the HCV RNA viral test, genotyping, and transient elastography. RESULTS: The prevalence of positive serology was 0.27%. Among 161 patients with positive anti-HCV serology, 124 (77%) were contacted, 109 (67.7%) were tested for HCV RNA viral load, and 69 (42.8%) had positive results. Genotype 1 (75%) was the most prevalent genotype. Among 65 patients (94.2%) who underwent transient elastography, 30 (46.2%) presented with advanced fibrosis. Additionally, of the 161 patients, 55 (34.1%) were referred for treatment, but only 39 (24.2%) complied, with 36 (22.4%) showing sustained virological response. By the end of the study, 16 patients were still awaiting to receive medication. CONCLUSIONS: The prevalence of HCV-positive patients was low in Brasilia, and the gaps in the cascade of care for these patients were significantly below the targets of HCV infection elimination. This study opens new avenues for eliminating HCV infection and suggests that partnerships with clinical laboratories to conduct anti-HCV tests are a useful strategy to improve HCV diagnosis. TRIAL REGISTRATION: Research Ethics Committee of the Faculty of Health Sciences of the University of Brasília - UNB (CAAE number 77818317.2.0000.0030) and by the Ethics Committee of the Health Science Teaching and Research Foundation - FEPECS/SES/DF (CAAE number 77818317.2.3001.5553).


Assuntos
Hepacivirus/isolamento & purificação , Hepatite C/diagnóstico , Hepatite C/epidemiologia , Programas de Rastreamento , Adulto , Idoso , Antivirais/uso terapêutico , Brasil/epidemiologia , Estudos Transversais , Feminino , Genótipo , Hepacivirus/efeitos dos fármacos , Hepacivirus/genética , Hepacivirus/imunologia , Hepatite C/tratamento farmacológico , Hepatite C/virologia , Humanos , Cirrose Hepática/epidemiologia , Cirrose Hepática/patologia , Masculino , Programas de Rastreamento/estatística & dados numéricos , Pessoa de Meia-Idade , Prevalência , Testes Sorológicos , Resposta Viral Sustentada
14.
Mol Biol Rep ; 47(8): 6463-6469, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32712854

RESUMO

Cystic fibrosis (CF) is a genetic disease caused by variants in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. There are over 2,000 different pathogenic and non-pathogenic variants described in association with a broad clinical heterogeneity. In this work, we identified a novel variant S511Lfs*2 in CFTR gene that has not been reported in patients with CF. The patient was a female genotyped with c.1000C>T (legacy name: R334W) variant (pathogenic, CF-causing) and the novel variant (S511Lfs*2). We verified the amino acid sequence, the protein structure, and predicted the pathogenicity employing computational analysis. Our findings showed that S511Lfs*2 is a frameshift variant and suggest that it is associated with severe CF phenotype, as it leads to a lack of CFTR protein synthesis, and consequently the loss of its functional activity.


Assuntos
Regulador de Condutância Transmembrana em Fibrose Cística/genética , Fibrose Cística/genética , Mutação da Fase de Leitura , Adulto , Feminino , Humanos , Fenótipo , Adulto Jovem
15.
Skeletal Radiol ; 49(7): 1037-1049, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32147757

RESUMO

The anterolateral ligament (ALL) and capsule of the knee are anatomical structures involved in rotational stability and pivot-shift control. As such, it has been demonstrated that the extra-articular anterolateral procedures improve clinical outcome when performed as an augmentation of the anterior cruciate ligament (ACL) reconstruction in specific groups of patients. This review describes the postoperative imaging findings of two techniques used to perform these procedures, using magnetic resonance imaging (MRI), computed tomography (CT), and radiography. The first technique described is the lateral extra-articular tenodesis (LET), which uses a strip of the iliotibial band that is harvested, passed underneath the lateral collateral ligament (LCL) and fixed posterior, and proximal to the lateral femoral epicondyle (LFE), preserving ITB insertion on Gerdy's tubercle. The second technique described is the ALL reconstruction, a procedure that attempts to recreate the anatomy of the ALL, using most often a gracilis autograft. In this procedure, femoral fixation is performed proximal and posterior to the LFE, and tibial fixation is slightly distal to the joint line, halfway from Gerdy's tubercle to the fibular head. The main objective of this review is to provide an overview of the postoperative imaging aspects of these two procedures with MRI, CT, and radiography and to describe possible complications. As they become more common, it is important for the radiologist and the orthopedic surgeon to understand their particularities in combination with the already well-known ACL reconstruction.


Assuntos
Reconstrução do Ligamento Cruzado Anterior/métodos , Articulação do Joelho/diagnóstico por imagem , Articulação do Joelho/cirurgia , Tenodese/métodos , Humanos , Imageamento por Ressonância Magnética , Tomografia Computadorizada por Raios X , Raios X
16.
Skeletal Radiol ; 49(5): 723-730, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31807873

RESUMO

OBJECTIVE: To determine if ulnar variance can be evaluated by magnetic resonance (MR) imaging and if this measure can be used as a reliable indicator when correlated to the gold standard technique, conventional radiography (CR). MATERIALS AND METHODS: From January to July 2018, the MR images of 64 participants, comprising 66 wrists (mean age 34.9 years; 33 females; 31 males), were obtained. Among those, 29 were referred for evaluation of the wrist for different medical reasons and 35 were asymptomatic volunteers from our radiology group. All subjects had a plain radiography of the wrist in a posteroanterior view with a mean interval between images of 1 day. Local ethics committee approved the study and written informed consent was obtained from all patients. Two musculoskeletal radiologists evaluated the images. Correlation coefficients and a linear regression model were used for statistical analyses. RESULTS: Intra- and inter-observer analyses were performed for both diagnostic methods with results showing concordance (intra-observer: kappa score: MR 0.915/CR 0.931; p < 0.05; inter-observer: kappa score: MR 0.857/CR 0.931; p < 0.05). The intraclass correlations of MR and CR to evaluate agreement between the radiologists was slightly higher for radiologist #1 (0.771) than for radiologist #2 (0.659). A linear regression model showed good model fit indicating that MR does correlate with the ulnar variance as measured by CR (CR = 0.554 + 0.897 × MR, R2 = 0.665). CONCLUSION: Although CR is the gold standard method for the evaluation of ulnar variance, our study demonstrated that MR can be used as a reliable qualitative option.


Assuntos
Pesos e Medidas Corporais/métodos , Imageamento por Ressonância Magnética/métodos , Ulna/anatomia & histologia , Punho/anatomia & histologia , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Adulto Jovem
17.
Acta Neurochir (Wien) ; 162(12): 3189-3196, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32591949

RESUMO

BACKGROUND: There is no consensus about which type of imaging study, computed tomography myelography (CTM) or magnetic resonance imaging (MRI), provides better information concerning root avulsion in adult brachial plexus injuries. METHODS: Patients with upper brachial plexus traumatic injuries underwent both CTM and MRI and surgical exploration. The imaging studies were analyzed by two independent radiologists and the data were compared with the intraoperative findings. The statistical analysis was based on dichotomous classification of the nerve roots (normal or altered). The interobserver agreement was assessed using Cohen's Kappa. The accuracy of CTM and MRI in comparison with the intraoperative findings was evaluated using the same methodology. RESULTS: Fifty-two adult patients were included. CTM tended to yield slightly higher percentages of alterations than MRI The interobserver agreement was better on CTM than on MRI for all nerve roots: C5, 0.9960 (strong) vs. 0.145 (poor); C6, 0.970 (strong) vs. 0.788 (substantial); C7, 0.969 (strong) vs. 0.848 (strong). The accuracy regarding the intraoperative findings was also higher on CTM (moderate, kappa 0.40-0.59) than on MRI (minimal, kappa 0.20-0.39) for all nerve roots. Accordingly, the overall percentage concordance (both normal or both altered) was superior in the CTM evaluation (approx. 70-75% vs. 60-65%). CTM was superior for both sensitivity and specificity at all nerve roots. CONCLUSION: CTM had greater interobserver agreement and higher diagnostic accuracy than MRI in adult patients with root avulsions due to brachial plexus injury.


Assuntos
Plexo Braquial/diagnóstico por imagem , Plexo Braquial/lesões , Radiculopatia/diagnóstico por imagem , Adolescente , Adulto , Plexo Braquial/cirurgia , Feminino , Humanos , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Mielografia , Sensibilidade e Especificidade , Tomografia Computadorizada por Raios X , Adulto Jovem
18.
Arthroscopy ; 36(6): 1679-1686, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32061976

RESUMO

PURPOSE: To evaluate the magnetic resonance imaging (MRI) characteristics of Segond fractures, including the structures attached to the avulsed fragment, the integrity of anterolateral ligament (ALL) and iliotibial band (ITB), and fragment size and location. METHODS: This was a retrospective, cross-sectional study with MRI scans from 2016 to 2019 with the term "Segond" in the reports, signs of acute trauma, and a bony anterolateral tibial avulsion (Segond) fracture. Two experienced observers accessed images to evaluate fragment dimensions (anteroposterior, mediolateral, and craniocaudal) and distances from anatomic landmarks (Gerdy's tubercle, articular surface, and posterolateral tibial corner). ALLs and ITBs also were evaluated, both for integrity and for attachment to the avulsed bony fragment. Data were statistically evaluated for significant correlations. RESULTS: Forty-eight knee MRIs of patients suffering from a combined anterior cruciate ligament and Segond injury were evaluated. The ALL presented with edema in 28 cases (58.3%) and was torn in 3 cases (6.3%). The ALL was attached to the bone fragment in all cases and the ITB also was attached in 25 cases (52.1%). Receiver operating characteristic curves also demonstrated that the larger each of the dimensions and the volume of the fragment, the greater the probability of ITB fibers being inserted. Also, the narrower the distance from the fragment to the center of Gerdy's tubercle, the greater the probability of iliotibial band fibers being attached. Interobserver correlation varied from 0.87 to 0.97 for all measurements. CONCLUSIONS: In all combined ACL injuries and Segond fracture MRI cases, the complete ALL inserted on the avulsed bony fragment, whereas the posterior part of the ITB was only attached in 52.1% of the cases. Segond fracture fragment size was predictive for the structures attached to it. The ALL was abnormal in 64.5% of cases and presented a clear discontinuity in 6.3%. LEVEL OF EVIDENCE: Case Series, Level IV.


Assuntos
Lesões do Ligamento Cruzado Anterior/diagnóstico , Ligamento Cruzado Anterior/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Fraturas da Tíbia/diagnóstico , Adolescente , Adulto , Idoso , Lesões do Ligamento Cruzado Anterior/etiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fraturas da Tíbia/complicações , Adulto Jovem
19.
Sensors (Basel) ; 20(17)2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32825224

RESUMO

Photovoltaic (PV) energy use has been increasing recently, mainly due to new policies all over the world to reduce the application of fossil fuels. PV system efficiency is highly dependent on environmental variables, besides being affected by several kinds of faults, which can lead to a severe energy loss throughout the operation of the system. In this sense, we present a Monitoring System (MS) to measure the electrical and environmental variables to produce instantaneous and historical data, allowing to estimate parameters that ar related to the plant efficiency. Additionally, using the same MS, we propose a recursive linear model to detect faults in the system, while using irradiance and temperature on the PV panel as input signals and power as output. The accuracy of the fault detection for a 5 kW power plant used in the test is 93.09%, considering 16 days and around 143 hours of faults in different conditions. Once a fault is detected by this model, a machine-learning-based method classifies each fault in the following cases: short-circuit, open-circuit, partial shadowing, and degradation. Using the same days and faults applied in the detection module, the accuracy of the classification stage is 95.44% for an Artificial Neural Network (ANN) model. By combining detection and classification, the overall accuracy is 92.64%. Such a result represents an original contribution of this work, since other related works do not present the integration of a fault detection and classification approach with an embedded PV plant monitoring system, allowing for the online identification and classification of different PV faults, besides real-time and historical monitoring of electrical and environmental parameters of the plant.

20.
BMC Genomics ; 20(1): 581, 2019 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-31299887

RESUMO

BACKGROUND: Biological adhesion (bioadhesion), enables organisms to attach to surfaces as well as to a range of other targets. Bioadhesion evolved numerous times independently and is ubiquitous throughout the kingdoms of life. To date, investigations have focussed on various taxa of animals, plants and bacteria, but the fundamental processes underlying bioadhesion and the degree of conservation in different biological systems remain poorly understood. This study had two aims: 1) To characterise tissue-specific gene regulation in the pedal disc of the model cnidarian Exaiptasia pallida, and 2) to elucidate putative genes involved in pedal disc adhesion. RESULTS: Five hundred and forty-seven genes were differentially expressed in the pedal disc compared to the rest of the animal. Four hundred and twenty-seven genes were significantly upregulated and 120 genes were significantly downregulated. Forty-one condensed gene ontology terms and 19 protein superfamily classifications were enriched in the pedal disc. Eight condensed gene ontology terms and 11 protein superfamily classifications were depleted. Enriched superfamilies were consistent with classifications identified previously as important for the bioadhesion of unrelated marine invertebrates. A host of genes involved in regulation of extracellular matrix generation and degradation were identified, as well as others related to development and immunity. Ab initio prediction identified 173 upregulated genes that putatively code for extracellularly secreted proteins. CONCLUSION: The analytical workflow facilitated identification of genes putatively involved in adhesion, immunity, defence and development of the E. pallida pedal disc. When defence, immunity and development-related genes were identified, those remaining corresponded most closely to formation of the extracellular matrix (ECM), implicating ECM in the adhesion of anemones to surfaces. This study therefore provides a valuable high-throughput resource for the bioadhesion community and lays a foundation for further targeted research to elucidate bioadhesion in the Cnidaria.


Assuntos
Perfilação da Expressão Gênica , Anêmonas-do-Mar/anatomia & histologia , Anêmonas-do-Mar/genética , Transcrição Gênica , Animais , Ontologia Genética , Especificidade de Órgãos , Regulação para Cima
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