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1.
Addict Biol ; 19(1): 37-48, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22978678

RESUMO

Studies of adolescent drug use show (1) a pattern in which the use of tobacco precedes the use of other drugs and (2) a positive relationship between adolescent tobacco use and later drug use. These observations have led to the hypothesis that a causal relationship exists between early exposure to nicotine and the later use of hard drugs such as cocaine. Using male C57BL/6J mice, we tested the hypothesis that nicotine exposure in adolescence leads to increased intravenous self-administration (IVSA) of cocaine in adulthood. Using miniature osmotic pumps, we exposed mice and their littermate controls to nicotine (24 mg/kg/day) or vehicle, respectively, over the entire course of adolescence [postnatal days (P) 28-56]. Nicotine exposure was terminated on P56 and mice were not exposed to nicotine again during the experiment. On P73, mice were allowed to acquire cocaine IVSA (1.0 mg/kg/infusion) and a dose-response curve was generated (0.18, 0.32, 0.56, 1.0, 1.8 mg/kg/infusion). Lever pressing during extinction conditions was also evaluated. All mice rapidly learned to lever press for the combination of cocaine infusions and non-drug stimuli. Analysis of the dose-response curve revealed that adolescent nicotine-exposed mice self-administered significantly more (P < 0.05) cocaine than controls at all but the highest dose. No significant differences were observed between adolescent nicotine-exposed and control mice during the acquisition or extinction stages. These results indicate that adolescent nicotine exposure can increase cocaine IVSA in mice, which suggests the possibility of a causal link between adolescent tobacco use and later cocaine use in humans.


Assuntos
Cocaína/administração & dosagem , Inibidores da Captação de Dopamina/administração & dosagem , Comportamento de Procura de Droga/efeitos dos fármacos , Nicotina/farmacologia , Adolescente , Comportamento do Adolescente/efeitos dos fármacos , Idade de Início , Análise de Variância , Animais , Peso Corporal/efeitos dos fármacos , Cateterismo , Transtornos Relacionados ao Uso de Cocaína/epidemiologia , Transtornos Relacionados ao Uso de Cocaína/etiologia , Condicionamento Operante/efeitos dos fármacos , Cotinina/sangue , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Extinção Psicológica/efeitos dos fármacos , Humanos , Bombas de Infusão Implantáveis , Masculino , Camundongos , Camundongos Endogâmicos C57BL/genética , Nicotina/administração & dosagem , Distribuição Aleatória , Ratos , Autoadministração/estatística & dados numéricos , Tabagismo/complicações , Tabagismo/epidemiologia
2.
Cerebellum ; 12(4): 547-56, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23436049

RESUMO

Imaging, clinical, and pre-clinical studies have provided ample evidence for a cerebellar involvement in cognitive brain function including cognitive brain disorders, such as autism and schizophrenia. We previously reported that cerebellar activity modulates dopamine release in the mouse medial prefrontal cortex (mPFC) via two distinct pathways: (1) cerebellum to mPFC via dopaminergic projections from the ventral tegmental area (VTA) and (2) cerebellum to mPFC via glutamatergic projections from the mediodorsal and ventrolateral thalamus (ThN md and vl). The present study compared functional adaptations of cerebello-cortical circuitry following developmental cerebellar pathology in a mouse model of developmental loss of Purkinje cells (Lurcher) and a mouse model of fragile X syndrome (Fmr1 KO mice). Fixed potential amperometry was used to measure mPFC dopamine release in response to cerebellar electrical stimulation. Mutant mice of both strains showed an attenuation in cerebellar-evoked mPFC dopamine release compared to respective wildtype mice. This was accompanied by a functional reorganization of the VTA and thalamic pathways mediating cerebellar modulation of mPFC dopamine release. Inactivation of the VTA pathway by intra-VTA lidocaine or kynurenate infusions decreased dopamine release by 50 % in wildtype and 20-30 % in mutant mice of both strains. Intra-ThN vl infusions of either drug decreased dopamine release by 15 % in wildtype and 40 % in mutant mice of both strains, while dopamine release remained relatively unchanged following intra-ThN md drug infusions. These results indicate a shift in strength towards the thalamic vl projection, away from the VTA. Thus, cerebellar neuropathologies associated with autism spectrum disorders may cause a reduction in cerebellar modulation of mPFC dopamine release that is related to a reorganization of the mediating neuronal pathways.


Assuntos
Cerebelo/metabolismo , Transtornos Globais do Desenvolvimento Infantil/metabolismo , Modelos Animais de Doenças , Dopamina/metabolismo , Rede Nervosa/metabolismo , Plasticidade Neuronal/fisiologia , Córtex Pré-Frontal/metabolismo , Animais , Cerebelo/efeitos dos fármacos , Transtornos Globais do Desenvolvimento Infantil/genética , Infusões Intraventriculares , Ácido Cinurênico/administração & dosagem , Lidocaína/administração & dosagem , Masculino , Camundongos , Camundongos da Linhagem 129 , Camundongos Endogâmicos CBA , Camundongos Knockout , Camundongos Mutantes Neurológicos , Rede Nervosa/efeitos dos fármacos , Plasticidade Neuronal/efeitos dos fármacos , Córtex Pré-Frontal/efeitos dos fármacos
3.
Synapse ; 65(11): 1204-12, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21638338

RESUMO

Cerebellar involvement in autism, schizophrenia, and other cognitive disorders is typically associated with prefrontal cortical pathology. However, the underlying neuronal mechanisms are largely unknown. It has previously been shown in mice that stimulation of the dentate nucleus (DN) of the cerebellum evokes dopamine (DA) release in the medial prefrontal cortex (mPFC). Here, we investigated the neuronal circuitry by which the cerebellum modulates mPFC DA release. Fixed potential amperometry was used to determine the contribution of two candidate pathways by which the cerebellum may modulate mPFC DA release. In urethane anesthetized mice, DA release evoked by DN stimulation (50 Hz) was recorded in mPFC following local anesthetic lidocaine (0.02 µg) or ionotropic glutamate receptor antagonist kynurenate (0.5 µg) infusions into the mediodorsal or ventrolateral thalamic nucleus (ThN md; ThN vl), or the ventral tegmental area (VTA). Following intra-VTA lidocaine or kynurenate infusions, DA release was decreased by ∼50%. Following intra-ThN md and ThN vl infusions of either drug, DA release was decreased by ∼35% and 15%, respectively. Reductions in DA release following lidocaine or kynurenate infusions were not significantly different indicating that neuronal cells in the VTA and ThN were activated primarily if not entirely by glutamatergic inputs. The present study suggests that neuropathological changes in the cerebellum commonly observed in autism, schizophrenia, and other cognitive disorders could result in a loss of functionality of cerebellar-mPFC circuitry that is manifested as aberrant dopaminergic activity in the mPFC. Additionally, these results specifically implicate glutamate as a modulator of mPFC dopaminergic activity.


Assuntos
Cerebelo/metabolismo , Cognição/fisiologia , Dopamina/metabolismo , Córtex Pré-Frontal/metabolismo , Animais , Humanos , Camundongos , Camundongos Endogâmicos CBA , Camundongos Mutantes Neurológicos , Rede Nervosa/metabolismo , Vias Neurais/metabolismo
4.
Neurobiol Learn Mem ; 94(2): 220-8, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20566377

RESUMO

Although behavioral inflexibility and Purkinje cell loss are both well established in autism, it is unknown if these phenomena are causally related. Using a mouse model, we tested the hypothesis that developmental abnormalities of the cerebellum, including Purkinje cell loss, result in behavioral inflexibility. Specifically, we made aggregation chimeras (Lc/+<-->+/+) between lurcher (Lc/+) mutant embryos and wildtype (+/+) control embryos. Lurcher mice lose 100% of their Purkinje cells postnatally, while chimeric mice lose varying numbers of Purkinje cells. We tested these mice on the acquisition and serial reversals of an operant conditional visual discrimination, a test of behavioral flexibility in rodents. During reversals 1 and 2, all groups of mice committed similar numbers of "perseverative" errors (those committed while session performance was <= 40% correct). Lurchers, however, committed a significantly greater number of "learning" errors (those committed while session performance was between 41% and 85% correct) than both controls and chimeras, and most were unable to advance past reversal 3. During reversals 3 and 4, chimeras, as a group, committed more "perseverative", but not "learning" errors than controls, although a comparison of Purkinje cell number and performance in individual mice revealed that chimeras with fewer Purkinje cells made more "learning" errors and had shorter response latencies than chimeras with more Purkinje cells. These data suggest that developmental cerebellar Purkinje cell loss may affect higher level cognitive processes which have previously been shown to be mediated by the prefrontal cortex, and are commonly deficient in autism spectrum disorders.


Assuntos
Transtorno Autístico/patologia , Condicionamento Operante/fisiologia , Aprendizagem por Discriminação/fisiologia , Função Executiva/fisiologia , Células de Purkinje/patologia , Reversão de Aprendizagem/fisiologia , Análise de Variância , Animais , Transtorno Autístico/complicações , Transtorno Autístico/fisiopatologia , Comportamento Animal/fisiologia , Contagem de Células , Morte Celular , Doenças Cerebelares/complicações , Doenças Cerebelares/patologia , Doenças Cerebelares/fisiopatologia , Cerebelo/crescimento & desenvolvimento , Cerebelo/patologia , Cerebelo/fisiologia , Quimera/crescimento & desenvolvimento , Quimera/fisiologia , Modelos Animais de Doenças , Camundongos , Camundongos Mutantes Neurológicos , Reconhecimento Visual de Modelos/fisiologia , Estimulação Luminosa , Células de Purkinje/fisiologia , Tempo de Reação/fisiologia , Aprendizagem Seriada/fisiologia
5.
Neurosci Lett ; 730: 135027, 2020 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-32437898

RESUMO

Multiple lines of evidence implicate the serotonin (5-HT) system in social function, including biomarker findings in autism spectrum disorder. In mice, knock-in of a rare Gly56Ala substitution in the serotonin transporter (SERT) causes elevated whole blood 5-HT levels, increased 5-HT clearance in the brain, and altered social and repetitive behavior. To further examine the molecular impact of this variant on social response, SERT Ala56 mutant mice and wildtype littermate controls were exposed to a social or non-social stimulus. We examined the differential activation of the prefrontal cortex, lateral amygdala, and medial amygdala, to social stimuli through RNA sequencing. Differentially expressed genes were enriched in axonal guidance signaling pathways, networks related to nervous system development and function, neurological and psychiatric disorders, and behavior. These identified pathways and networks may shed light on the molecular cascades underlying the impact of altered SERT function on social behavior.


Assuntos
Transtorno do Espectro Autista/metabolismo , Encéfalo/crescimento & desenvolvimento , Expressão Gênica/fisiologia , Neurônios/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Animais , Modelos Animais de Doenças , Masculino , Camundongos , Serotonina/metabolismo , Comportamento Social
6.
Autism Res ; 12(5): 732-743, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30977597

RESUMO

The K-Cl cotransporter KCC2 is essential in the development of the "GABA switch" that produces a change in neuronal responses to GABA signaling from excitatory to inhibitory early in brain development, and alterations in this progression have previously been hypothesized to play a causal role in autism spectrum disorder (ASD). We investigated the KCC2b (Slc12a5) heterozygous knockout mouse using a battery of rodent behavioral tests relevant to core and comorbid ASD symptoms. Compared to wild-type littermates, KCC2+/- mice were normal in standard measures of locomotor activity, grooming and digging behaviors, and social, vocalization, and anxiety-like behaviors. However, KCC2+/- mice exhibited increased social dominance behaviors and increased amplitude of spontaneous postsynaptic currents in the medial prefrontal cortex (PFC) that were previously implicated in governing social hierarchy and dominance behaviors. Treatment of wild-type mouse brain slices with the KCC2 inhibitor VU0240511 increased the amplitude and frequency of excitatory postsynaptic currents, partially recapitulating the phenotype of KCC2+/- mice. These findings indicate that the activity of KCC2 plays a role in social dominance, in parallel with effects on PFC signaling, further suggesting that KCC2 function has some relevance to social behavior but without the breadth of impact on autism-like behavior suggested by previous studies. Further testing could assess whether KCC2 alters other circuits and whether additional factors such as environmental insults may precipitate autism-related behavioral phenotypes. Autism Research 2019, 12: 732-743. © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: A mouse model of altered chloride transporter expression was used to look for a role in behaviors and brain function relevant to autism. There was an imbalance in signaling in the prefrontal cortex, and increased social dominance behavior, although other autism-related behaviors were not changed. These findings indicate that altered chloride transporter function affects prefrontal cortex function and social dominance without a broader impact on autism-like behaviors.


Assuntos
Transtorno Autístico/fisiopatologia , Comportamento Animal/fisiologia , Fenômenos Eletrofisiológicos/fisiologia , Neurônios/fisiologia , Córtex Pré-Frontal/fisiopatologia , Predomínio Social , Animais , Modelos Animais de Doenças , Masculino , Camundongos , Camundongos Knockout
7.
Mol Autism ; 8: 30, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28649315

RESUMO

BACKGROUND: People with fragile X syndrome (FXS) often have deficits in social behavior, and a substantial portion meet criteria for autism spectrum disorder. Though the genetic cause of FXS is known to be due to the silencing of FMR1, and the Fmr1 null mouse model representing this lesion has been extensively studied, the contributions of this gene and its protein product, FMRP, to social behavior are not well understood. METHODS: Fmr1 null mice and wildtype littermates were exposed to a social or non-social stimulus. In one experiment, subjects were assessed for expression of the inducible transcription factor c-Fos in response to the stimulus, to detect brain regions with social-specific activity. In a separate experiment, tissue was taken from those brain regions showing differential activity, and RNA sequencing was performed. RESULTS: Immunohistochemistry revealed a significantly greater number of c-Fos-positive cells in the lateral amygdala and medial amygdala in the brains of mice exposed to a social stimulus, compared to a non-social stimulus. In the prelimbic cortex, there was no significant effect of social stimulus; although the number of c-Fos-positive cells was lower in the social condition compared to the non-social condition, and negatively correlated with c-Fos in the amygdala. RNA sequencing revealed differentially expressed genes enriched for molecules known to interact with FMRP and also for autism-related genes identified in the Simons Foundation Autism Research Initiative gene database. Ingenuity Pathway Analysis detected enrichment of differentially expressed genes in networks and pathways related to neuronal development, intracellular signaling, and inflammatory response. CONCLUSIONS: Using the Fmr1 null mouse model of fragile X syndrome, we have identified brain regions, gene networks, and molecular pathways responsive to a social stimulus. These findings, and future experiments following up on the role of specific gene networks, may shed light on the neural mechanisms underlying dysregulated social behaviors in fragile X syndrome and more broadly.


Assuntos
Tonsila do Cerebelo/metabolismo , Modelos Animais de Doenças , Proteína do X Frágil da Deficiência Intelectual/genética , Síndrome do Cromossomo X Frágil/genética , Lobo Límbico/metabolismo , Tonsila do Cerebelo/fisiopatologia , Animais , Comportamento Animal , Biomarcadores/análise , Mapeamento Encefálico , Proteína do X Frágil da Deficiência Intelectual/metabolismo , Síndrome do Cromossomo X Frágil/diagnóstico , Síndrome do Cromossomo X Frágil/fisiopatologia , Deleção de Genes , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Humanos , Relações Interpessoais , Lobo Límbico/fisiopatologia , Masculino , Camundongos , Camundongos Knockout , Anotação de Sequência Molecular , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/metabolismo , Análise de Sequência de RNA , Transdução de Sinais
8.
Neuropsychopharmacology ; 42(2): 427-436, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27550733

RESUMO

Biomarker, neuroimaging, and genetic findings implicate the serotonin transporter (SERT) in autism spectrum disorder (ASD). Previously, we found that adult male mice expressing the autism-associated SERT Ala56 variant have altered central serotonin (5-HT) system function, as well as elevated peripheral blood 5-HT levels. Early in gestation, before midbrain 5-HT projections have reached the cortex, peripheral sources supply 5-HT to the forebrain, suggesting that altered maternal or placenta 5-HT system function could impact the developing embryo. We therefore used different combinations of maternal and embryo SERT Ala56 genotypes to examine effects on blood, placenta and embryo serotonin levels and neurodevelopment at embryonic day E14.5, when peripheral sources of 5-HT predominate, and E18.5, when midbrain 5-HT projections have reached the forebrain. Maternal SERT Ala56 genotype was associated with decreased placenta and embryonic forebrain 5-HT levels at E14.5. Low 5-HT in the placenta persisted, but forebrain levels normalized by E18.5. Maternal SERT Ala56 genotype effects on forebrain 5-HT levels were accompanied by a broadening of 5-HT-sensitive thalamocortical axon projections. In contrast, no effect of embryo genotype was seen in concepti from heterozygous dams. Blood 5-HT levels were dynamic across pregnancy and were increased in SERT Ala56 dams at E14.5. Placenta RNA sequencing data at E14.5 indicated substantial impact of maternal SERT Ala56 genotype, with alterations in immune and metabolic-related pathways. Collectively, these findings indicate that maternal SERT function impacts offspring placental 5-HT levels, forebrain 5-HT levels, and neurodevelopment.


Assuntos
Troca Materno-Fetal , Placenta/metabolismo , Prosencéfalo/embriologia , Prosencéfalo/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Serotonina/biossíntese , Animais , Feminino , Genótipo , Camundongos Endogâmicos , Camundongos Transgênicos , Gravidez , Rombencéfalo/metabolismo , Tálamo/embriologia , Tálamo/metabolismo
9.
Front Syst Neurosci ; 7: 15, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23717269

RESUMO

Autism spectrum disorders are a group of neurodevelopmental disorders characterized by deficits in social skills and communication, stereotyped and repetitive behavior, and a range of deficits in cognitive function. While the etiology of autism is unknown, current research indicates that abnormalities of the cerebellum, now believed to be involved in cognitive function and the prefrontal cortex (PFC), are associated with autism. The current paper proposes that impaired cerebello-cortical circuitry could, at least in part, underlie autistic symptoms. The use of animal models that allow for manipulation of genetic and environmental influences are an effective means of elucidating both distal and proximal etiological factors in autism and their potential impact on cerebello-cortical circuitry. Some existing rodent models of autism, as well as some models not previously applied to the study of the disorder, display cerebellar and behavioral abnormalities that parallel those commonly seen in autistic patients. The novel findings produced from research utilizing rodent models could provide a better understanding of the neurochemical and behavioral impact of changes in cerebello-cortical circuitry in autism.

10.
Toxicol Sci ; 136(1): 144-53, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23912914

RESUMO

Exposure to polychlorinated biphenyls (PCBs) alters brain dopamine (DA) concentrations and DA receptor/transporter function, suggesting the reinforcing properties of drugs of abuse acting on the DA system may be affected by PCB exposure. Female Long-Evans rats were orally exposed to 0, 3, or 6 mg/kg/day PCBs from 4 weeks prior to breeding until litters were weaned on postnatal day 21. In vivo fixed potential amperometry (FPA) was used in adult anesthetized offspring to determine whether perinatal PCB exposure altered (1) presynaptic DA autoreceptor (DAR) sensitivity, (2) electrically evoked nucleus accumbens (NAc) DA efflux following administration of cocaine, and (3) the rate of depletion of presynaptic DA stores. One adult male and female littermate were tested using FPA following a single injection of cocaine (20 mg/kg ip), whereas a second adult male and female littermate were tested following the last of seven daily cocaine injections of the same dose. The carbon fiber recording microelectrode was positioned in the NAc core, and DA oxidation currents (i.e., DA release) evoked by brief stimulation of the medial forebrain bundle (MFB) were quantified before and after administration of cocaine. PCB-exposed rats exhibited enhanced stimulation-evoked DA release (relative to baseline) following a single injection of cocaine. Although nonexposed controls exhibited typical DA sensitization following repeated cocaine administration, this effect was attenuated in PCB-exposed rats. In addition, DAR sensitivity was higher (males only), and the rate of depletion of presynaptic DA stores was greater in PCB-exposed animals relative to nonexposed controls. These results indicate that perinatal PCB exposure can modify DA synaptic transmission in the NAc in a manner previously shown to alter the reinforcing properties of cocaine.


Assuntos
Estimulantes do Sistema Nervoso Central/toxicidade , Cocaína/toxicidade , Dopamina/metabolismo , Núcleo Accumbens/efeitos dos fármacos , Bifenilos Policlorados/toxicidade , Transmissão Sináptica/efeitos dos fármacos , Animais , Animais Recém-Nascidos , Autorreceptores/efeitos dos fármacos , Autorreceptores/metabolismo , Relação Dose-Resposta a Droga , Estimulação Elétrica , Feminino , Masculino , Exposição Materna , Núcleo Accumbens/metabolismo , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Terminações Pré-Sinápticas/efeitos dos fármacos , Terminações Pré-Sinápticas/metabolismo , Ratos , Ratos Long-Evans , Receptores Dopaminérgicos/efeitos dos fármacos , Receptores Dopaminérgicos/metabolismo , Fatores de Tempo , Desmame
11.
Psychopharmacology (Berl) ; 215(4): 631-42, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21212937

RESUMO

RATIONALE: The tendency to use cocaine is determined by genetic and environmental effects across the lifespan. One critical environmental effect is early drug exposure, which is both driven by and interacts with genetic background. The mesoaccumbens dopamine system, which is critically involved in the rewarding properties of drugs of abuse, undergoes significant development during adolescence, and thus may be at particular risk to repeated nicotine exposure during this period, thereby establishing vulnerability for subsequent adult psychostimulant use. OBJECTIVES: We tested the hypotheses that adolescent nicotine exposure results in attenuation of the enhancing effects of cocaine on medial forebrain bundle (MFB) electrical stimulation-evoked dopamine release in the nucleus accumbens shell (AcbSh) in adulthood and that this effect is significantly influenced by genotype. METHODS: Mice from the progenitor strains C57BL/6J and DBA/2J and those from the BXD20/TyJ and BXD86/RwwJ recombinant inbred lines were exposed to nicotine via osmotic minipumps from postnatal day (P) 28 to P56. When mice reached P70, dopamine functional dynamics in AcbSh was evaluated by means of in vivo fixed potential amperometry in combination with electrical stimulation of mesoaccumbens dopaminergic axons in the MFB. RESULTS: Adolescent exposure to nicotine in all strains dose-dependently reduced the ability of a fixed-dose challenge injection of cocaine (10 mg/kg, i.p.) to enhance MFB electrical stimulation-evoked dopamine release in AcbSh in adults. The magnitude of this effect was genotype-dependent. CONCLUSIONS: These results suggest a genotype-dependent mechanism by which nicotine exposure during adolescence causes persistent changes in the sensitivity to "hard" stimulants such as cocaine.


Assuntos
Cocaína/farmacologia , Dopamina/metabolismo , Nicotina/farmacologia , Núcleo Accumbens/efeitos dos fármacos , Recompensa , Caracteres Sexuais , Envelhecimento/efeitos dos fármacos , Envelhecimento/genética , Animais , Transtornos Relacionados ao Uso de Cocaína/etiologia , Transtornos Relacionados ao Uso de Cocaína/genética , Transtornos Relacionados ao Uso de Cocaína/metabolismo , Feminino , Genótipo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Núcleo Accumbens/crescimento & desenvolvimento , Núcleo Accumbens/metabolismo , Especificidade da Espécie
12.
CNS Neurosci Ther ; 16(3): 137-62, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20370804

RESUMO

Dopaminergic neurons in the substantia nigra pars compacta and ventral tegmental area of the midbrain form the nigrostriatal and mesocorticolimbic dopaminergic pathways that, respectively, project to dorsal and ventral striatum (including prefrontal cortex). These midbrain dopaminergic nuclei and their respective forebrain and cortical target areas are well established as serving a critical role in mediating voluntary motor control, as evidenced in Parkinson's disease, and incentive-motivated behaviors and cognitive functions, as exhibited in drug addiction and schizophrenia, respectively. Although it cannot be disputed that excitatory and inhibitory amino acid-based neurotransmitters, such as glutamate and GABA, play a vital role in modulating activity of midbrain dopaminergic neurons, recent evidence suggests that acetylcholine may be as important in regulating dopaminergic transmission. Midbrain dopaminergic cell tonic and phasic activity is closely dependent upon projections from hindbrain pedunculopontine and the laterodorsal tegmental nuclei, which comprises the only known cholinergic inputs to these neurons. In close coordination with glutamatergic and GABAergic activity, these excitatory cholinergic projections activate nicotinic and muscarinic acetylcholine receptors within the substantia nigra and ventral tegmental area to modulate dopamine transmission in the dorsal/ventral striatum and prefrontal cortex. Additionally, acetylcholine-containing interneurons in the striatum also constitute an important neural substrate to provide further cholinergic modulation of forebrain striatal dopaminergic transmission. In this review, we examine neurological and psychopathological conditions associated with dysfunctions in the interaction of acetylcholine and dopamine and conventional and new pharmacological approaches to treat these disorders.


Assuntos
Acetilcolina/metabolismo , Doenças do Sistema Nervoso Central/tratamento farmacológico , Doenças do Sistema Nervoso Central/fisiopatologia , Dopamina/metabolismo , Animais , Dopaminérgicos/uso terapêutico , Interações Medicamentosas , Humanos , Modelos Biológicos , Vias Neurais/fisiologia , Receptores Muscarínicos/metabolismo , Substância Negra/fisiologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-19964298

RESUMO

In this study, fixed potential amperometry was used to examine several pathways by which Deep Brain Stimulation (DBS) of the subthalamic nucleus (STN) or dopamine axons within the dorsal forebrain bundle (DFB) release striatal dopamine, thus potentially providing therapeutic benefits for Parkinson's Disease patients. In urethane anesthetized mice, electrical stimulations (20 monophasic pulses at 50 Hz every 30 sec) were applied to the STN or DFB while infusing the local anesthetic lidocaine (4%) into the substantia nigra compacta (SNc) or pedunculopontine tegmental nucleus (PPT). Findings suggest that DFB stimulation activates ascending SNc dopamine axons, while STN stimulation evokes striatal dopamine release directly via excitatory glutamatergic inputs to SNc dopamine cells and indirectly via excitatory cholinergic/glutamatergic STN-PPT-SNc pathways.


Assuntos
Estimulação Encefálica Profunda , Neurônios/patologia , Doença de Parkinson/terapia , Núcleo Subtalâmico/patologia , Animais , Axônios/patologia , Eletrodos Implantados , Humanos , Lidocaína/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Núcleo Tegmental Pedunculopontino/patologia , Receptores Colinérgicos/metabolismo , Processamento de Sinais Assistido por Computador , Substância Negra/patologia
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