Detoxification of areca nut acid hydrolysate and production of xylitol by Candida tropicalis (MTCC 6192).

Areca nut husk is the most promising alternative source of low-cost raw materials because it contains a considerable amount of five-carbon monosaccharide sugar in the form of xylose. This polymeric sugar can be isolated and transformed into a value-added chemical using fermentation. To extract sugars from areca nut husk fibers, preliminary pretreatment, such as dilute acid hydrolysis (H2SO4), was performed. The hemicellulosic hydrolysate of areca nut husk can produce xylitol through fermentation, but toxic components inhibit the growth of microorganisms. To overcome this, a series of detoxification treatments, including pH adjustment, activated charcoal, and ion exchange resin, were carried out to reduce the concentration of inhibitors in the hydrolysate. This study reports a remarkable 99% removal of inhibitors in the hemicellulosic hydrolysate. Subsequently, a fermentation process using Candida tropicalis (MTCC6192) was executed with the detoxified hemicellulosic hydrolysate of areca nut husk, yielding an optimum xylitol yield of 0.66 g/g. This study concludes that detoxification techniques like pH adjustment, activated charcoal, and ion exchange resins are the most economical and effective methods for eliminating toxic compounds in hemicellulosic hydrolysates. Therefore, the medium derived after detoxification from areca nut hydrolysate may be considered to have significant potential for xylitol production.

Enhanced Algal Biomass Production in a Novel Electromagnetic Photobioreactor (E-PBR).

Enhancement in algal biomass production is a crucial parameter for generating value-added chemicals from renewable carbon sources. This study aimed to fabricate a novel photobioreactor with integrated and innovative electromagnets to assess microalgae's growth under the magnetic field's effect. The present study observed the effect of magnetic field exposure on the microalgae Chlorella sorokiniana cultivation and estimated the change in pigments with growth. In addition, the carbohydrate content of Chlorella sorokiniana was also evaluated. Chlorella sorokiniana culture, when exposed to a magnetic field strength of 10 mT d-1 resulted in a greater biomass concentration and productivity of about 12% and 8%, respectively, higher than the control culture. A positive effect of magnetic field exposure was also observed in the microalga's pigments concentration and carbohydrate content. The chlorophyll-a content increased twofold exponentially with the growth and the carbohydrate content of the microalgae cells also increased by 15% compared to the control culture. Therefore, the novel Electromagnetic Photobioreactor (E-PBR) can be used to produce enhanced biomass and other value-added products.

Therapeutic Potential of HMF and Its Derivatives: a Computational Study.

Over the past century, chemicals and energy have increasingly been derived from non-renewable resources. The growing demand for essential chemicals and shrinking inventory make reliable, sustainable sources essential. Carbohydrates offer by far the greatest carbon supply. Furan compounds, a particular family of dehydration products, are believed to offer high chemical potential. Here, we analyze 5-HMF (5, hydroxymethylfurfural) and some of its derivatives in particular, a furan-type platform chemical. To analyze the therapeutic potential of HMF and its derivatives, this study utilized cutting-edge technologies such as computer-aided drug design, virtual screening, molecular docking, and molecular dynamic simulation. We conducted 189 docking simulations and examined some of the most promising dock poses using the molecular dynamic simulator. As for the receptors for our compounds, the leading candidates are human acetylcholinesterase, beta-lactamases, P. aeruginosa LasR, and S. aureus tyrosyl-tRNA synthetases. Out of all derivatives considered in this study, 2,5-furandicarboxylic acid (FCA) performed best.

A mini review on microwave and contemporary based biohydrogen production technologies: a comparison.

Hydrogen gas, along with conventional fossil fuels, has been used as a green fuel with enormous potential. Due to the rapid depletion of fossil fuels, a new dimension of hydrogen production technology has arrived to reduce reliance on nonrenewable energy sources. Microwave-based hydrogen production is a more promising and cost-effective technology than other existing green hydrogen production methods such as fermentation and gasification. Microwave heating may be superior to traditional heating due to several advantages such as less power consumption compared to other methods, higher yield, and a higher rate of conversion. Compared to another process for hydrogen production, the microwave-driven process worked efficiently at lower temperatures by providing more than 70% yield. The process of production can be optimized by using properly sized biomass, types of biomass, water flow, temperature, pressure, and reactor size. This method is the most suitable, attractive, and efficient technique for hydrogen production in the presence of a suitable catalyst. Hot spots formed by microwave irradiation would have a substantial impact on the yield and properties of microwave-processed goods. The current techno-economic situation of various technologies for hydrogen production is discussed here, with cost, efficiency, and durability being the most important factors to consider. The present review shows that a cost-competitive hydrogen economy will necessitate continual efforts to increase performance, scale-up, technical prospects, and political backing.

A Review of Green Synthesis of Metal Nanoparticles Using Algae.

The ability of algae to accumulate metals and reduce metal ions make them a superior contender for the biosynthesis of nanoparticles and hence they are called bio-nano factories as both the live and dead dried biomass are used for the synthesis of metallic nanoparticles. Microalgae, forming a substantial part of the planet's biodiversity, are usually single-celled colony-forming or filamentous photosynthetic microorganisms, including several legal divisions like Chlorophyta, Charophyta, and Bacillariophyta. Whole cells of Plectonema boryanum (filamentous cyanobacteria) proved efficient in promoting the production of Au, Ag, and Pt nanoparticles. The cyanobacterial strains of Anabaena flos-aquae and Calothrix pulvinate were used to implement the biosynthesis of Au, Ag, and Pt nanoparticles. Once synthesized within the cells, the nanoparticles were released into the culture media where they formed stable colloids easing their recovery. Lyngbya majuscule and Chlorella vulgaris have been reported to be used as a cost-effective method for Ag nanoparticle synthesis. Dried edible algae (Spirulina platensis) was reported to be used for the extracellular synthesis of Au, Ag, and Au/Ag bimetallic nanoparticles. Synthesis of extracellular metal bio-nanoparticles using Sargassum wightii and Kappaphycus alvarezi has also been reported. Bioreduction of Au (III)-Au (0) using the biomass of brown alga, Fucus vesiculosus, and biosynthesis of Au nanoparticles using red algal (Chondrus crispus) and green algal (Spyrogira insignis) biomass have also been reported. Algae are relatively convenient to handle, less toxic, and less harmful to the environment; synthesis can be carried out at ambient temperature and pressure and in simple aqueous media at a normal pH value. Therefore, the study of algae-mediated biosynthesis of metallic nanoparticles can be taken toward a new branch, termed phyco-nanotechnology.

Application of microbial extracellular carbohydrate polymeric substances in food and allied industries.

Extracellular polymeric substances (EPS) are biopolymers, composed of polysaccharides, nucleic acids, proteins and lipids, which possess unique functional properties. Despite significant strides made in chemical production processes for polymers, the niche occupied by exopolysaccharides produced by bacteria, yeast or algae is steadily growing in its importance. With the availability of modern tools, a lot of information has been generated on the physico-chemical and biological properties using spectrometric tools, while advanced microscopic techniques have provided valuable insights into the structural-functional aspects. The size of EPS generally ranges between 10 and 10,000 kDa. The wide spectra of applications of EPS as adhesives, stabilizer, gelling, suspending, thickening agent, and surfactants in food and pharmaceutical industries are observed. The health benefits of these EPS enable the improvement of dual function, added value, and green products. This review summarizes previous work on the structural composition, rheological and thermal behaviour, and biosynthetic pathways of EPS and bioprocesses developed for their production. This review also considers each of the above factors and presents the current knowledge on the importance and refinement of available downstream protocols and genetic engineering towards specific food applications, which can help to diversify their prospects in different food and allied industries.

Optimization of Process Parameters for Production of Pectinase using Bacillus Subtilis MF447840.1.

BACKGROUND: Pectinase enzyme has immense industrial prospects in the food and beverage industries. OBJECTIVE: In our investigation, we find out the optimum process parameters suitable for better pectinase generation by Bacillus subtilis MF447840.1 using submerged fermentation. METHOD: 2% (OD600 nm = 0.2) of pure Bacillus subtilis MF447840.1 bacterial culture was inoculated in sterile product production media. The production media components used for this study were 1 g/l of pectin, 2 g/l of (NH4)2SO4, 1 g/l of NaCl, 0.25 g/l of K2HPO4, 0.25 g/l of KH2PO4 and 1 g/l of MgSO4 for pectinase generation. We reviewed all recent patents on pectinase production and utilization. The various process parameters were observed by changing one variable time method. RESULTS: The optimum fermentation condition of different parameters was noticed to be 5% inoculums, 25% volume ratio, temperature (37°C), pH (7.4) and agitation rate (120 rpm) following 4 days incubation. CONCLUSION: Maximum pectinase generation was noticed as 345 ± 12.35 U following 4 days incubation.

Tamarind kernel powder: a novel agro-residue for the production of cellobiose dehydrogenase under submerged fermentation by Termitomyces clypeatus.

The study investigates the potential of substitution of the conventional carbohydrate nutrient (cellulose) in media with cheap agro-residues for cellobiose dehydrogenase production by Termitomyces clypeatus (CDHtc) under submerged conditions. Different agro-residues tested for enzyme production were characterized using FTIR and XRD analysis. As CDHtc production was highest with tamarind kernel powder (TKP), it was selected for process optimizations through shake-flask fermentations. The optimized parameters were then applied to batch cultures in a 5 L bioreactor that gave enzyme yield (57.4 U mL⁻¹) similar to that obtained under shake-flask fermentations (57.05 U mL⁻¹). The study also made an attempt to predict CDHtc production with respect to time of fermentation and mycelial growth. The specific growth rate and carrying capacity of the mycelia were also determined, and the values lie in the ranges of 0.024-0.027 h⁻¹ and 7.2-7.1 mg mL⁻¹, respectively.