Insights into the molecular basis of hypergravity-induced root growth phenotype in bread wheat (Triticum aestivum L.).

Hypergravity is a condition where the force of gravity exceeds that on the surface of the Earth and can be simulated by centrifugation. Previously, a significant increase in root growth phenotype was observed when wheat seeds were exposed to hypergravity (10 g for 12 h). In the present study, we investigated the molecular basis of this change through root transcriptome. The data revealed a total of 3765 up-regulated and 2102 down-regulated transcripts in response to hypergravity. GO enrichment analysis revealed hormonal responses, cell division, and cell-wall-related terms were significantly enriched in hypergravity. The increased isoform level expression of transcripts involved in auxin biosynthesis, transport, and signaling was observed. Further, enhanced expression of cell division transcripts and down-regulation of cell number regulator genes suggests rapid cell division. Overexpression of cellulose and hemicellulose biosynthesis transcripts suggests demand for cell-wall constituents. Collectively, this study identified candidate genes associated with hypergravity-induced enhanced root growth.

Plant responses to hypergravity: a comprehensive review.

MAIN CONCLUSION: Hypergravity is an effective novel stimulus to elucidate plant gravitational and mechanobiological behaviour. Here, we review the current understanding of phenotypic, physio-biochemical, and molecular plant responses to simulated hypergravity. Plants readily respond to altered gravity conditions, such as microgravity or hypergravity. Hypergravity-a gravitational force higher than that on the Earth's surface (> 1g)-can be simulated using centrifuges. Exposing seeds, seedlings, or plant cell cultures to hypergravity elicits characteristic morphological, physio-biochemical, and molecular changes. While several studies have provided insights into plant responses and underlying mechanisms, much is still elusive, including the interplay of hypergravity with gravitropism. Moreover, hypergravity is of great significance for mechano- and space/gravitational biologists to elucidate fundamental plant behaviour. In this review, we provide an overview of the phenotypic, physiological, biochemical, and molecular responses of plants to hypergravity. We then discuss the involvement of hypergravity in plant gravitropism-the directional growth along the gravity vector. Finally, we highlight future research directions to expand our understanding of hypergravity in plant biology.

Plant responses to real and simulated microgravity.

Plant biology experiments in real and simulated microgravity have significantly contributed to our understanding of physiology and behavior of plants. How do plants perceive microgravity? How that perception translates into stimulus? And in turn plant's response and adaptation to microgravity through physiological, cellular, and molecular changes have been reasonably well documented in the literature. Knowledge gained through these plant biology experiments in microgravity helped to successfully cultivate crops in space. For instance, salad crop such as red romaine lettuce grown on the International Space Station (ISS) is allowed to incorporate into the crew's supplementary diet. However, the use of plants as a sustainable bio-regenerative life support system (BLSS) to produce fresh food and O2, reduce CO2 level, recycle metabolic waste, and efficient water management for long-duration space exploration missions requires critical gap filling research. Hence, it is inevitable to reflect and review plant biology microgravity research findings time and again with a new set of data available in the literature. With that in focus, the current article discusses phenotypic, physiological, biochemical, cell cycle, cell wall changes and molecular responses of plants to microgravity both in real and simulated conditions with the latest literature.

Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.).

Hypergravity-an evolutionarily novel environment has been exploited to comprehend the response of living organisms including plants in the context of extra-terrestrial applications. Recently, researchers have shown that hypergravity induces desired phenotypic variability in seedlings. In the present study, we tested the utility of hypergravity as a novel tool in inducing reliable phenotype/s for potential terrestrial crop improvement applications. To investigate, bread wheat seeds (UAS-375 genotype) were subjected to hypergravity treatment (10×g for 12, and 24 h), and evaluated for seedling vigor and plant growth parameters in both laboratory and greenhouse conditions. It was also attempted to elucidate the associated biochemical and hormonal changes at different stages of vegetative growth. Resultant data revealed that hypergravity treatment (10×g for 12 h) significantly enhanced root length, root volume, and root biomass in response to hypergravity. The robust seedling growth phenotype may be attributed to increased alpha-amylase and TDH enzyme activities observed in seeds treated with hypergravity. Elevated total chlorophyll content and Rubisco (55 kDa) protein expression across different stages of vegetative growth in response to hypergravity may impart physiological benefits to wheat growth. Further, hypergravity elicited robust endogenous phytohormones dynamics in root signifying altered phenotype/s. Collectively, this study for the first time describes the utility of hypergravity as a novel tool in inducing reliable root phenotype that could be potentially exploited for improving wheat varieties for better water usage management.