A Study to Measure Sexual Dysfunction among Women of Reproductive Age Group with Diabetes Mellitus Attending the Outpatient Department of a Tertiary Care Hospital in Kolkata.

Sexual dysfunction in Diabetes is an under-investigated problem, especially in women. The research barriers surrounding the issue of female sexual dysfunction are manifold and difficult to surpass, especially in the context of the Asian Subcontinent, where a multitude of sociocultural factors suppress the expression of distress. Early screening, diagnosis, and counselling can help in managing female sexual dysfunction in Diabetes Mellitus and positively impact the lives of many women. This study was undertaken to find the prevalence of sexual dysfunction among diabetic women of reproductive age group, its relation to BMI, duration of Diabetes and microvascular complications, and to find its sociodemographic associations. MATERIAL: A descriptive, observational study was carried out over a period of 1 year. 250 purposively sampled women attending the diabetic outpatient department of a tertiary care hospital were enrolled. All were known diabetic, between the ages of 30 and 44 years. A detailed history was obtained with the aid of a case record form which included the FSFI (Female Sexual Function Index), and a demographic questionnaire, after proper explanation in their own local vernacular as per their literacy level. Available medical records were used for finding the duration of diabetes, and associated diabetes related microvascular complications. OBSERVATION: Prevalence of FSD among the study population was 73.6%. A significant association was found between age and sexual dysfunction (p<0.05). It was found to be more among the Hindus, compared to the Muslims (p<0.05). There was a significant association with BMI (>25kg/m2), increasing duration of Diabetes, and the presence of microvascular complications (p<0.05). In fact, all patients with microvascular complications were experiencing some degree of sexual dysfunction. No association was found with socioeconomic status, literacy, addiction, occupation or type of family. CONCLUSION: Female sexual health issues in Diabetes are a neglected area, both in the clinic as well as in research, yet sexual health problems in this group may be more common than previously recognised. Low FSFI scores are associated with increasing age, higher BMI and increasing duration of Diabetes. Future studies may identify appropriate ways to probe and explore the related issues for early screening, and help establish the role of newer treatments.

Detection of a large spectrum of viral infections in conjunctival premalignant and malignant lesions.

To study the interaction between HIV and other carcinogenic infections in conjunctival squamous cell carcinoma (SCC), we evaluated the presence of a broad spectrum of human viruses in conjunctiva specimens. Beta Human papillomavirus (HPV; n = 46), gamma HPV (n = 52), polyomaviruses (n = 12) and herpes viruses (n = 3) was determined in DNA extracted from 67 neoplastic and 55 non-neoplastic conjunctival tissues of HIV-positive and HIV negative subjects by Luminex-based assays. Next-generation sequencing (NGS) was also used to further characterize the presence of cutaneous HPVs. Detection of beta-2 HPV infections was associated with the risk of neoplasia (adjusted odds ratio [aOR] 3.0; 95% confidence interval [CI] 1.3-6.8), regardless of HIV status (HIV positive, aOR 2.6, 95% CI 0.9-7.7; HIV negative, aOR 3.5, 95% CI 0.9-14.4). EBV was strongly associated with the risk of neoplasia (aOR 12.0, 95% CI 4.3-33.5; P < .01) mainly in HIV individuals (HIV positive, aOR 57.5; 95% CI: 10.1-327.1; HIV negative aOR 2.6; 95% CI: 0.2-34.7). NGS allowed to identify 13 putative novel HPVs in cases and controls. Our findings suggest a role of beta HPV types and EBV, in conjunctival SCC. However, additional studies of viral expression in tumor tissue are required to confirm the causal association.

Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples.

BACKGROUND: Although the role of viral agents, such as human papillomavirus (e.g. HPV16, HPV18) in colorectal cancer (CRC) has been previously investigated, results remain inconclusive. METHODS: To further evaluate the involvement of oncogenic HPV types in CRC, 40 frozen neoplastic and 40 adjacent colonic tissues collected from Italian patients were analyzed by Luminex-based assays that detect a broad spectrum of HPV types, i.e. Alpha (n = 21), Beta (n = 46) and Gamma HPVs (n = 52). In addition, 125 frozen CRC samples and 70 surrounding mucosal tissues were collected from Czech patients and analyzed by broad spectrum PCR protocols: (i) FAP59/64, (ii) FAPM1 and (iii) CUT combined with Next Generation Sequencing (NGS). RESULTS: Using Luminex-basedassays, DNA from HPV16 was detected in 5% (2/40) CRC tissues from Italian patients. One HPV16 DNA-positive CRC case was subsequently confirmed positive for E6*I mRNA. Cutaneous beta HPV types were detected in 10% (4/40) adjacent tissues only, namely HPV111 (n = 3) and HPV120 (n = 1), while gamma HPV168 (n = 1) and HPV199 (n = 1) types were detected in adjacent and in tumor tissues, respectively. The NGS analysis of the CRC Czech samples identified HPV sequences from mucosal alpha-3 (HPV89), alpha-7 (HPV18, 39, 68 and 70) and alpha-10 species (HPV11), as well as cutaneous beta-1 (HPV20, 24, 93, 98, 105,124) beta-2 (HPV23), beta-3 (HPV49) and gamma-1 species (HPV205). CONCLUSIONS: Our findings indicate that HPV types belonging to the mucosal alpha, and the 'cutaneous' beta and gamma genera can be detected in the colonic mucosal samples with a low prevalence rate and a low number of HPV reads by Luminex and NGS, respectively. However, additional studies are required to corroborate these findings.

Biotransformation of xenobiotics by hairy roots.

The exponential industrial growth we see today rides on the back of large scale production of chemicals, explosives and pharmaceutical products. However, the effluents getting released from their manufacturing units are greatly compromising the sustainability of our environment. With greater awareness of the imperative for environmental clean-up, a promising approach that is attracting increasing research interests is biodegradation of xenobiotics. In this approach, biotransformation has proven to be one of the most effective tools. While many different model frameworks have been used to study different aspects of biotransformation, hairy roots (HRs) have been found to be exceptionally valuable. HR cultures are preferred over other in-vitro model systems due to their biochemical stability and hormone-autotrophy. In addition, the multi-enzyme biosynthetic potential of HRs which is similar to the parent plant and their relatively low-cost cultural requirements further characterize their suitability for biotransformation. The recent progress observed in scale-up of HR cultures and understanding of functional genomics has opened up new dimensions providing valuable insights for industrial application. This review article summarizes the potential of HR cultures in the biotransformation of xenobiotics, their limitations in the application on a large scale and current strategies to alleviate them. Advancement in bioreactors engineering enabling large scale cultivation and modern gene technologies improving biotransformation efficiency promises to extend laboratory results to industrial applications.