Silicon-nanoparticles loaded biochar for soil arsenic immobilization and alleviation of phytotoxicity in barley: Implications for human health risk.

Arsenic (As)-induced environmental pollution and associated health risks are recognized on a global level. Here the impact of cotton shells derived biochar (BC) and silicon-nanoparticles loaded biochar (nano-Si-BC) was explored on soil As immobilization and its phytotoxicity in barley plants in a greenhouse study. The barley plants were grown in a sandy loam soil with varying concentrations of BC and nano-Si-BC (0, 1, and 2%), along with different levels of As (0, 5, 10, and 20 mg kg-1). The FTIR spectroscopy, SEM-EDX, and XRD were used to characterize BC and nano-Si-BC. Results revealed that As treatment had a negative impact on barley plant development, grain yield, physiology, and anti-oxidative response. However, the addition of nano-Si-BC led to a 71% reduction in shoot As concentration compared to the control with 20 mg kg-1 of As, while BC alone resulted in a 51% decline. Furthermore, the 2% nano-Si-BC increased grain yield by 94% compared to control and 28% compared to BC. The addition of 2% nano-Si-BC to As-contaminated soil reduced oxidative stress (34% H2O2 and 48% MDA content) and enhanced plant As tolerance (92% peroxidase and 46% Ascorbate peroxidase activity). The chlorophyll concentration in barley plants decreased due to oxidative stress. Additionally, the incorporation of 2% nano-Si-BC resulted in a 76% reduction in water soluble and NaHCO3 extractable As. It is concluded that the use of BC or nano-Si-BC in As contaminated soil for barley resulted in a low human health risk (HQ < 1), as it effectively immobilized As and promoted higher activity of antioxidants.

Copper uptake, essentiality, toxicity, detoxification and risk assessment in soil-plant environment.

Copper (Cu) is an essential metal for human, animals and plants, although it is also potentially toxic above supra-optimal levels. In plants, Cu is an essential cofactor of numerous metalloproteins and is involved in several biochemical and physiological processes. However, excess of Cu induces oxidative stress inside plants via enhanced production of reactive oxygen species (ROS). Owing to its dual nature (essential and a potential toxicity), this metal involves a complex network of uptake, sequestration and transport, essentiality, toxicity and detoxification inside the plants. Therefore, it is vital to monitor the biogeo-physiochemical behavior of Cu in soil-plant-human systems keeping in view its possible essential and toxic roles. This review critically highlights the latest understanding of (i) Cu adsorption/desorption in soil (ii) accumulation in plants, (iii) phytotoxicity, (iv) tolerance mechanisms inside plants and (v) health risk assessment. The Cu-mediated oxidative stress and resulting up-regulation of several enzymatic and non-enzymatic antioxidants have been deliberated at molecular and cellular levels. Moreover, the role of various transporter proteins in Cu uptake and its proper transportation to target metalloproteins is critically discussed. The review also delineates Cu build-up in plant food and accompanying health disorders. Finally, this review proposes some future perspectives regarding Cu biochemistry inside plants. The review, to a large extent, presents a complete picture of the biogeo-physiochemical behavior of Cu in soil-plant-human systems supported with up-to-date 10 tables and 5 figures. It can be of great interest for post-graduate level students, scientists, industrialists, policymakers and regulatory authorities.