Being versus appearing smart: Children's developing intuitions about how reputational motives guide behavior.

Children engage in reputation management to appear favorably to others. The present studies explore when children use reputational motives to predict others' behavior. Four- to 9-year-old children (N = 576; 53% female; approximately 60% White) heard stories about two kids: one who cares about being competent, and one who cares about appearing competent. Across five studies, with age, children predicted the reputationally motivated child would be more likely to lie to cover up failure (OR = 1.97) but less likely to seek help in public (vs. private; OR = 0.53) or downplay success (OR = 0.66). With age, children also liked this character less (OR = 0.56). Implications of these findings for children's reputation management and achievement motivation are discussed.

The temporal pattern and lifestyle associations of respiratory virus infection in a cohort study spanning the first two years of life.

BACKGROUND: Respiratory virus infection is common in early childhood, and children may be symptomatic or symptom-free. Little is known regarding the association between symptomatic/asymptomatic infection and particular clinical factors such as breastfeeding as well as the consequences of such infection. METHOD: We followed an unselected cohort of term neonates to two years of age (220 infants at recruitment, 159 who remained in the study to 24 months), taking oral swabs at birth and oropharyngeal swabs at intervals subsequently (at 1.5, 6, 9, 12, 18 and 24 months and in a subset at 3 and 4.5 months) while recording extensive metadata including the presence of respiratory symptoms and breastfeeding status. After 2 years medical notes from the general practitioner were inspected to ascertain whether doctor-diagnosed wheeze had occurred by this timepoint. Multiplex PCR was used to detect a range of respiratory viruses: influenza (A&B), parainfluenza (1-4), bocavirus, human metapneumovirus, rhinovirus, coronavirus (OC43, 229E, NL63, HKU1), adenovirus, respiratory syncytial virus (RSV), and polyomavirus (KI, WU). Logistic regression and generalised estimating equations were used to identify associations between clinical factors and virus detection. RESULTS: Overall respiratory viral incidence increased with age. Rhinovirus was the virus most frequently detected. The detection of a respiratory virus was positively associated with respiratory symptoms, male sex, season, childcare and living with another child. We did not observe breastfeeding (whether assessed as the number of completed months of breastfeeding or current feed status) to be associated with the detection of a respiratory virus. There was no association between early viral infection and doctor-diagnosed wheeze by age 2 years. CONCLUSION: Asymptomatic and symptomatic viral infection is common in the first 2 years of life with rhinovirus infection being the most common. Whilst there was no association between early respiratory viral infection and doctor-diagnosed wheeze, we have not ruled out an association of early viral infections with later asthma, and long-term follow-up of the cohort continues.

A linkage and exome study of multiplex families with bipolar disorder implicates rare coding variants of ANK3 and additional rare alleles at 10q11-q21.

Background: Bipolar disorder is a highly heritable psychiatric condition for which specific genetic factors remain largely unknown. In the present study, we used combined whole-exome sequencing and linkage analysis to identify risk loci and dissect the contribution of common and rare variants in families with a high density of illness. Methods: Overall, 117 participants from 15 Australian extended families with bipolar disorder (72 with affective disorder, including 50 with bipolar disorder type I or II, 13 with schizoaffective disorder-manic type and 9 with recurrent unipolar disorder) underwent whole-exome sequencing. We performed genome-wide linkage analysis using MERLIN and conditional linkage analysis using LAMP. We assessed the contribution of potentially functional rare variants using a genebased segregation test. Results: We identified a significant linkage peak on chromosome 10q11-q21 (maximal single nucleotide polymorphism = rs10761725; exponential logarithm of the odds [LODexp] = 3.03; empirical p = 0.046). The linkage interval spanned 36 protein-coding genes, including a gene associated with bipolar disorder, ankyrin 3 (ANK3). Conditional linkage analysis showed that common ANK3 risk variants previously identified in genome-wide association studies - or variants in linkage disequilibrium with those variants - did not explain the linkage signal (rs10994397 LOD = 0.63; rs9804190 LOD = 0.04). A family-based segregation test with 34 rare variants from 14 genes under the linkage interval suggested rare variant contributions of 3 brain-expressed genes: NRBF2 (p = 0.005), PCDH15 (p = 0.002) and ANK3 (p = 0.014). Limitations: We did not examine non-coding variants, but they may explain the remaining linkage signal. Conclusion: Combining family-based linkage analysis with next-generation sequencing data is effective for identifying putative disease genes and specific risk variants in complex disorders. We identified rare missense variants in ANK3, PCDH15 and NRBF2 that could confer disease risk, providing valuable targets for functional characterization.

CYLD is a causative gene for frontotemporal dementia - amyotrophic lateral sclerosis.

Frontotemporal dementia and amyotrophic lateral sclerosis are clinically and pathologically overlapping disorders with shared genetic causes. We previously identified a disease locus on chromosome 16p12.1-q12.2 with genome-wide significant linkage in a large European Australian family with autosomal dominant inheritance of frontotemporal dementia and amyotrophic lateral sclerosis and no mutation in known amyotrophic lateral sclerosis or dementia genes. Here we demonstrate the segregation of a novel missense variant in CYLD (c.2155A>G, p.M719V) within the linkage region as the genetic cause of disease in this family. Immunohistochemical analysis of brain tissue from two CYLD p.M719V mutation carriers showed widespread glial CYLD immunoreactivity. Primary mouse neurons transfected with CYLDM719V exhibited increased cytoplasmic localization of TDP-43 and shortened axons. CYLD encodes a lysine 63 deubiquitinase and CYLD cutaneous syndrome, a skin tumour disorder, is caused by mutations that lead to reduced deubiquitinase activity. In contrast with CYLD cutaneous syndrome-causative mutations, CYLDM719V exhibited significantly increased lysine 63 deubiquitinase activity relative to the wild-type enzyme (paired Wilcoxon signed-rank test P = 0.005). Overexpression of CYLDM719V in HEK293 cells led to more potent inhibition of the cell signalling molecule NF-κB and impairment of autophagosome fusion to lysosomes, a key process in autophagy. Although CYLD mutations appear to be rare, CYLD's interaction with at least three other proteins encoded by frontotemporal dementia and/or amyotrophic lateral sclerosis genes (TBK1, OPTN and SQSTM1) suggests that it may play a central role in the pathogenesis of these disorders. Mutations in several frontotemporal dementia and amyotrophic lateral sclerosis genes, including TBK1, OPTN and SQSTM1, result in a loss of autophagy function. We show here that increased CYLD activity also reduces autophagy function, highlighting the importance of autophagy regulation in the pathogenesis of frontotemporal dementia and amyotrophic lateral sclerosis.

Comprehensive cross-disorder analyses of CNTNAP2 suggest it is unlikely to be a primary risk gene for psychiatric disorders.

The contactin-associated protein-like 2 (CNTNAP2) gene is a member of the neurexin superfamily. CNTNAP2 was first implicated in the cortical dysplasia-focal epilepsy (CDFE) syndrome, a recessive disease characterized by intellectual disability, epilepsy, language impairments and autistic features. Associated SNPs and heterozygous deletions in CNTNAP2 were subsequently reported in autism, schizophrenia and other psychiatric or neurological disorders. We aimed to comprehensively examine evidence for the role of CNTNAP2 in susceptibility to psychiatric disorders, by the analysis of multiple classes of genetic variation in large genomic datasets. In this study we used: i) summary statistics from the Psychiatric Genomics Consortium (PGC) GWAS for seven psychiatric disorders; ii) examined all reported CNTNAP2 structural variants in patients and controls; iii) performed cross-disorder analysis of functional or previously associated SNPs; and iv) conducted burden tests for pathogenic rare variants using sequencing data (4,483 ASD and 6,135 schizophrenia cases, and 13,042 controls). The distribution of CNVs across CNTNAP2 in psychiatric cases from previous reports was no different from controls of the database of genomic variants. Gene-based association testing did not implicate common variants in autism, schizophrenia or other psychiatric phenotypes. The association of proposed functional SNPs rs7794745 and rs2710102, reported to influence brain connectivity, was not replicated; nor did predicted functional SNPs yield significant results in meta-analysis across psychiatric disorders at either SNP-level or gene-level. Disrupting CNTNAP2 rare variant burden was not higher in autism or schizophrenia compared to controls. Finally, in a CNV mircroarray study of an extended bipolar disorder family with 5 affected relatives we previously identified a 131kb deletion in CNTNAP2 intron 1, removing a FOXP2 transcription factor binding site. Quantitative-PCR validation and segregation analysis of this CNV revealed imperfect segregation with BD. This large comprehensive study indicates that CNTNAP2 may not be a robust risk gene for psychiatric phenotypes.

(Un)common knowledge: Children use social relationships to determine who knows what.

Socially savvy individuals track what they know and what other people likely know, and they use this information to navigate the social world. We examine whether children expect people to have shared knowledge based on their social relationships (e.g., expecting friends to know each other's secrets, expecting members of the same cultural group to share cultural knowledge) and we compare children's reasoning about shared knowledge to their reasoning about common knowledge (e.g., the wrongness of moral violations). In three studies, we told 4- to 9-year-olds (N = 227) about what a child knew and asked who else knew the information: The child's friend (Studies 1-3), the child's schoolmate (Study 1), another child from the same national group (Study 2), or the child's sibling (Study 3). In all three studies, older children reliably used relationships to infer what other people knew. Moreover, with age, children increasingly considered both the type of knowledge and an individual's social relationships when reporting who knew what. The results provide support for a 'Selective Inferences' hypothesis and suggest that children's early attention to social relationships facilitates an understanding of how knowledge transfers - an otherwise challenging cognitive process.

When Children Treat Condemnation as a Signal: The Costs and Benefits of Condemnation.

Condemnation is ubiquitous in the social world and adults treat condemnation as a costly signal. We explore when children begin to treat condemnation as a signal by presenting 4- to 9-year-old children (N = 435) with stories involving a condemner of stealing and a noncondemner. Children were asked to predict who would be more likely to steal as well as who should be punished more harshly for stealing. In five studies, we found that 7- to 9-year-old children treat condemnation as a signal-thinking that a condemner is less likely to steal and should be punished more harshly if caught hypocritically stealing later. We discuss the implications of these results for children's emerging understanding of signaling and moral condemnation.

The effect of hunger on children's sharing behavior and fairness preferences.

How does feeling hungry affect children's sharing and evaluations of others' moral decision making? To examine this question, we gave 4- to 9-year-old children the opportunity to share resources with an anonymous other child and to evaluate third-party resource allocation decisions between hungry and full recipients. We also measured children's subjective reports of their own hunger, predicting that hungry children would be less generous in their own sharing and more likely to prefer distributions that favor the hungry recipient. Children's sharing increased with age, as did positive evaluations of equitable third-party distributions. Hungrier children were less likely to share overall, but particularly when sharing food-relevant resources. Hunger did not influence third-party resource allocation evaluations, and children still expected fairness from others even when behaving differently themselves.

Who are "we"? Dealing with conflicting moral obligations.

Satisfying one's obligations is an important part of being human. However, people's obligations can often prescribe contradictory behaviors. Moral obligations conflict (loyalty vs. fairness), and so do obligations to different groups (country vs. family when one is called to war). We propose that a broader framework is needed to account for how people balance different social and moral obligations.

Truncating variant burden in high-functioning autism and pleiotropic effects of LRP1 across psychiatric phenotypes

Background: Previous research has implicated de novo and inherited truncating mutations in autism-spectrum disorder. We aim to investigate whether the load of inherited truncating mutations contributes similarly to high-functioning autism, and to characterize genes that harbour de novo variants in high-functioning autism. Methods: We performed whole-exome sequencing in 20 high-functioning autism families (average IQ = 100). Results: We observed no difference in the number of transmitted versus nontransmitted truncating alleles for high-functioning autism (117 v. 130, p = 0.78). Transmitted truncating and de novo variants in high-functioning autism were not enriched in gene ontology (GO) or Kyoto Encyclopedia of Genes and Genomes (KEGG) categories, or in autism-related gene sets. However, in a patient with high-functioning autism we identified a de novo variant in a canonical splice site of LRP1, a postsynaptic density gene that is a target for fragile X mental retardation protein (FRMP). This de novo variant leads to in-frame skipping of exon 29, removing 2 of 6 blades of the ß-propeller domain 4 of LRP1, with putative functional consequences. Large data sets implicate LRP1 across a number of psychiatric disorders: de novo variants are associated with autism-spectrum disorder (p = 0.039) and schizophrenia (p = 0.008) from combined sequencing projects; common variants using genome-wide association study data sets from the Psychiatric Genomics Consortium show gene-based association in schizophrenia (p = 6.6 × E−07) and in a meta-analysis across 7 psychiatric disorders (p = 2.3 × E−03); and the burden of ultra-rare pathogenic variants has been shown to be higher in autism-spectrum disorder (p = 1.2 × E−05), using whole-exome sequencing from 6135 patients with schizophrenia, 1778 patients with autism-spectrum disorder and 7875 controls. Limitations: We had a limited sample of patients with high-functioning autism, related to difficulty in recruiting probands with high cognitive performance and no family history of psychiatric disorders. Conclusion: Previous studies and ours suggest an effect of truncating mutations restricted to severe autism-spectrum disorder phenotypes that are associated with intellectual disability. We provide evidence for pleiotropic effects of common and rare variants in the LRP1 gene across psychiatric phenotypes.

Children use similarity, propinquity, and loyalty to predict which people are friends.

Friendship fundamentally shapes interactions, and predicting other people's affiliations is crucial for effectively navigating the social world. We investigated how 3- to 11-year-old children use three cues to reason about friendship: propinquity, similarity, and loyalty. In past work, researchers asked children to report on their own friendships and found a shift from an early focus on propinquity to a much later understanding of the importance of loyalty. Indeed, attention to loyalty was not standard until adolescence. Across four studies (total N = 900), we used a simpler method in which we asked children to make a forced-choice decision about which of two people a main character was better friends with. Although we replicated the finding that understanding the importance of loyalty increases with age, we also found evidence that even the youngest children tested (3- to 5-year-olds) can use loyalty to predict friendship. Thus, a sophisticated understanding of how social interactions unfold differently between friends and nonfriends may be evident by the preschool years. We also discuss interesting developmental differences in how children weigh the importance of each of these friendship cues.

Crime but not punishment? Children are more lenient toward rule-breaking when the "spirit of the law" is unbroken.

Even early in development, children understand how rules work, and they harshly evaluate rule violators. Furthermore, we know that adults make nuanced evaluations about rule violations; in many situations, they believe that it can be acceptable to violate the technical language of a rule (the "letter of a rule") if doing so does not violate the reason why the rule was created (the "spirit of the rule"). Distinguishing between the letter and spirit of a rule is critical for a developed normative understanding. We investigated whether and when children begin to believe that it is less wrong to violate the letter of a rule if one does not violate the rule's spirit. Participants (N = 246 4- to 10-year-olds) were asked to evaluate a rule-breaker who either violated the letter of a rule but not the spirit or violated both the letter and spirit of a rule. We found that by 4 years of age, children acknowledged that the rule had technically been broken in both cases, but their evaluations of the rule-breaker were much more lenient in the case where the spirit of the rule remained intact. We also found that children are increasingly more lenient in the case where the spirit of the rule remains intact case as they age, and they are increasingly harsher in the case where the spirit is violated. We discuss how these studies provide insight into early normative reasoning, including implications for future research on social cognitive development, rule learning, and legislative intent.

Microbubble-enhanced dielectric barrier discharge pretreatment of microcrystalline cellulose.

Cellulose recalcitrance is one of the major barriers in converting renewable biomass to biofuels or useful chemicals. A pretreatment reactor that forms a dielectric barrier discharge plasma at the gas-liquid interface of the microbubbles has been developed and tested to pretreat α-cellulose. Modulation of the plasma discharge provided control over the mixture of species generated, and the reactive oxygen species (mainly ozone) were found to be more effective in breaking-up the cellulose structure compared to that of the reactive nitrogen species. The effectiveness of pretreatment under different conditions was determined by measuring both the solubility of treated samples in sodium hydroxide and conversion of cellulose to glucose via enzymatic hydrolysis. Solutions pretreated under pH 3 buffer solutions achieved the best result raising the solubility from 17% to 70% and improving the glucose conversion from 24% to 51%. Under the best conditions, plasma-microbubble treatment caused pronounced crevices on the cellulose surface enhancing access to the reactive species for further breakdown of the structure and to enzymes for saccharification.

Lee Silverman Voice Treatment (LSVT)-BIG to improve motor function in people with Parkinson's disease: a systematic review and meta-analysis.

OBJECTIVE: The technique called Lee Silverman Voice Treatment (LSVT)-LOUD has previously been used to improve voice quality in people with Parkinson's disease. The objective of this study was to assess the effectiveness of an alternate intervention, LSVT-BIG (signifying big movements), to improve functional mobility. DESIGN: Systematic review with meta-analysis of randomized trials. DATA SOURCES: Medline, Embase, CINAHL, AgeLine, Scopus and Cochrane Library were searched from inception to September 2017 using multiple search terms related to Parkinson's disease and LSVT-BIG. REVIEW METHOD: Two researchers searched the literature for studies of the LSVT-BIG intervention of 16 sessions, delivered by a certified instructor over four weeks, to any other intervention. Outcomes related to functional ability were included. Study quality was appraised using the Cochrane Risk of Bias tool. RESULTS: Four studies were included, reporting on three randomized trials of 84 participants with mild Parkinson's disease. Compared to physiotherapy exercises, or a shorter training protocol, there was a significant improvement in motor function assessed with the Unified Parkinson's Disease Rating Scale part III (mean difference = -3.20, 95% confidence interval = -5.18 to -1.23) and a trend towards faster Timed Up and Go performance (mean difference = -0.47, 95% confidence interval = -0.99 to 0.06) and 10-metre walk test (mean difference = -0.53, 95% confidence interval = -1.07 to 0.01). CONCLUSION: Compared to shorter format LSVT-BIG or general exercise, LSVT-BIG was more effective at improving motor function. This provides preliminary, moderate quality evidence that amplitude-oriented training is effective in reducing motor impairments for people with mild Parkinson's disease.

Children use partial resource sharing as a cue to friendship.

Resource sharing is an important aspect of human society, and how resources are distributed can provide people with crucial information about social structure. Indeed, a recent partiality account of resource distribution suggested that people may use unequal partial resource distributions to make inferences about a distributor's social affiliations. To empirically test this suggestion derived from the theoretical argument of the partiality account, we presented 4- to 9-year-old children with distributors who gave out resources unequally using either a partial procedure (intentionally choosing which recipient would get more) or an impartial procedure (rolling a die to determine which recipient would get more) and asked children to make judgments about whom the distributor was better friends with. At each age tested, children expected a distributor who gave partially to be better friends with the favored recipient (Studies 1-3). Interestingly, younger children (4- to 6-year-olds) inferred friendship between the distributor and the favored recipient even in cases where the distributor used an impartial procedure, whereas older children (7- to 9-year-olds) did not infer friendship based on impartial distributions (Study 1). These studies demonstrate that children use third-party resource distributions to make important predictions about the social world and add to our knowledge about the developmental trajectory of understanding the importance of partiality in addition to inequity when making social inferences.

The Development of Inequity Aversion.

Children and adults respond negatively to inequity. Traditional accounts of inequity aversion suggest that as children mature into adults, they become less likely to endorse all forms of inequity. We challenge the idea that children have a unified concern with inequity that simply becomes stronger with age. Instead, we argue that the developmental trajectory of inequity aversion depends on whether the inequity is seen as fair or unfair. In three studies ( N = 501), 7- to 8-year-olds were more likely than 4- to 6-year-olds to create inequity that disadvantaged themselves-a fair type of inequity. In findings consistent with our theory, 7- to 8-year-olds were not more likely than 4- to 6-year-olds to endorse advantageous inequity (Study 1) or inequity created by third parties (Studies 2 and 3)-unfair types of inequity. We discuss how these results expand on recent accounts of children's developing concerns with generosity and partiality.

Whose idea is it anyway? The importance of reputation in acknowledgement.

Six-year-old children negatively evaluate plagiarizers just as adults do (Olson & Shaw, 2011), but why do they dislike plagiarizers? Children may think plagiarism is wrong because plagiarizing negatively impacts other people's reputations. We investigated this possibility by having 6- to 9-year-old children evaluate people who shared their own or other people's ideas (stories). In Experiment 1, we found that children consider it acceptable to retell someone else's story if the source is given credit for their story (improving the source's reputation), but not if the reteller claims credit for the story (steals credit away from someone else). Experiments 2 and 3 showed that children do not consider it bad to lie by giving someone else credit for one's own good story (improving someone else's reputation), but do consider it bad to give someone else credit for one's own bad story (improving one's own reputation at the expense of someone else's). Experiment 4 demonstrated that children think it is equally bad to take credit for someone else's idea for oneself as it is to take someone else's idea and give credit to someone else, suggesting that children dislike when others take credit away from someone else, regardless of whether or not it improves the plagiarizer's reputation. Our results suggest that children dislike plagiarism because it negatively affects others' reputations by taking credit away from them.

Equity or equality? Moral judgments follow the money.

Previous research emphasizes people's dispositions as a source of differences in moral views. We investigate another source of moral disagreement, self-interest. In three experiments, participants played a simple economic game in which one player divides money with a partner according to the principle of equality (same payoffs) or the principle of equity (pay-offs proportional to effort expended). We find, first, that people's moral judgment of an allocation rule depends on their role in the game. People not only prefer the rule that most benefits them but also judge it to be more fair and moral. Second, we find that participants' views about equality and equity change in a matter of minutes as they learn where their interests lie. Finally, we find limits to self-interest: when the justification for equity is removed, participants no longer show strategic advocacy of the unequal division. We discuss implications for understanding moral debate and disagreement.

Fairness as partiality aversion: the development of procedural justice.

Adults and children dislike inequity-people being paid unequally for equal work. However, adults will allow inequity if the inequity is determined using an impartial procedure, indicating that they value procedural justice. It is unknown whether children value procedural justice when distributing resources. We investigated whether 5- to 8-year-old children would willingly create inequity between two recipients if they could do so using an impartial procedure. In Experiment 1, children preferred to use an impartial procedure (spinning a wheel that gave both recipients an equal chance to get a resource) over a partial procedure (spinning a wheel that gave one recipient a much better chance to get the resource). In Experiments 2 and 3, children preferred to use the same impartial procedure to assign a resource to one of two recipients, even over an option of keeping things equal by throwing the resource in the trash. Importantly, children preferred to throw the resource in the trash to uphold equality when the only other option was a partial procedure. Older children showed a stronger aversion to using partial procedures than younger children. These results suggest that children value procedural justice increasingly during middle childhood and that their fairness concerns may be more about avoiding partiality than inequity per se.

The nonmeek inherit the earth: Children generalize dominance, but not submissiveness.

Children and even infants have clear intuitions about power early in development; they can infer who is dominant and subordinate from observing a single interaction. However, it is unclear what children infer about each individual's status from these interactions-do they think dominants and subordinates will maintain their status when interacting with novel partners? In three experiments, we investigate this question. Children (4- to 10-year-olds, N = 365) heard stories about a dominant and subordinate agent and predicted the dominant or subordinate agent's behavior with a novel agent. In all studies, we found that 7- to 10-year-olds generalized dominance, thinking the dominant would again be dominant or "in charge," both for social power (e.g., granting permission) in Study 1 and physical dominance (e.g., a fistfight) in Studies 2 and 3. Furthermore, although they believed dominant agents would win dominance contests (fistfights), they did not believe they would win contests unrelated to dominance (math contests). Younger children did not generalize social power (Study 1) but did generalize physical dominance (Studies 2 and 3). However, even for physical dominance, their generalizations were less selective (i.e., they believed the dominant would win fistfights and math contests). Notably, neither age group generalized an agent's submissiveness in any of the studies-they did not believe a subordinate agent would again be subordinate when paired with a novel partner. We discuss how these results extend past work on children's developing intuitions about dominance and prompt deeper questions about the inferences children draw from dominance interactions. (PsycInfo Database Record (c) 2024 APA, all rights reserved).