RESUMO
Settlement and metamorphosis of pediveliger larvae of Mytilus coruscus in response to natural biofilms was investigated in the laboratory. Pediveliger larvae settled and metamorphosed in response to biofilms and post-larval settlement and metamorphosis increased with biofilm age. The activity of the biofilm was positively correlated with biofilm age, dry weight, bacterial density and diatom density, but had no apparent relationship with chlorophyll a concentration. The change in bacterial community composition corresponding to biofilm age may explain differences in the age-dependent inducing activities of biofilms, which in turn may play an important role in larval settlement in this species.
Assuntos
Biofilmes/crescimento & desenvolvimento , Metamorfose Biológica/fisiologia , Mytilus/fisiologia , Animais , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Diatomáceas/crescimento & desenvolvimento , Ecossistema , Larva/fisiologia , Fatores de TempoRESUMO
The complete mitochondrial genome sequence of Vaginulus alte and Homoiodoris japonica are described first in the article. The mitogenomes (14 772 bp and 14 601 bp) contain 22 tRNA genes, two ribosomal RNA genes, and 13 protein-coding genes, and one putative control region (CR). CR is not well characterized due to the lack of discrete conserved sequence blocks. This characteristic is similar with CRs of other invertebrate mitochondrial genomes, which is the typical bivalvia mitochondrial gene composition.
Assuntos
Gastrópodes/genética , Genoma Mitocondrial , Mitocôndrias/genética , Análise de Sequência de DNA/métodos , Animais , Composição de Bases , DNA Ribossômico/genética , Gastrópodes/classificação , Tamanho do Genoma , Filogenia , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome sequence of Platevindex sp. is firstly described in the article. The mitogenome (13,908 bp) contains 22 tRNA genes, 2 ribosomal RNA genes and 13 protein-coding genes, and 1 putative control region (CR). CR is not well characterized due to lack of discrete conserved sequence blocks. This characteristic is similar with CRs of other invertebrate mitochondrial genomes. The characteristic is the typical bivalvia mitochondrial gene composition.
Assuntos
DNA Mitocondrial/genética , Gastrópodes/genética , Genoma Mitocondrial/genética , Mitocôndrias/genética , Animais , Composição de Bases/genética , Sequência de Bases , Tamanho do Genoma , Dados de Sequência Molecular , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
We generated complete mitochondrial genome sequences data for 4 genera (Onchidium, Platevindex, Paraoncidium and Peronia) in Onchidiidae to construct a phylogenetic tree in conjunction with other 9 existing data among gastropods. The topology showed that the taxa clustered into two main groups of four species, one of which included Onchidium struma and the Platevindex mortoni, the other Paraoncidium reevesii and Peronia verruculata. The process in Pulmonata from sea to land in accordance with the evolution of respiratory organs from branchial gills to pulmonary cavity has been shown. This will also constitute a framework for phylogeny evolution analysis, systematic classfication of Onchidiidae and other euthyneurans (pulmonates and opisthobranchs).
Assuntos
Gastrópodes/classificação , Gastrópodes/genética , Genoma Mitocondrial , Filogenia , Animais , Composição de Bases , Evolução Molecular , Genes Mitocondriais , Tamanho do Genoma , Genômica , Fases de Leitura Aberta , Sequências Reguladoras de Ácido Nucleico , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
The complete mitochondrial genome sequence of Peronia verruculata is firstly described in the article. The mitogenome (13,861 bp) contains 22 tRNA genes, 2 ribosomal RNA genes and 13 protein-coding genes. The characteristic is the typical bivalvia mitochondrial gene composition.
Assuntos
Gastrópodes/genética , Genoma Mitocondrial , Genômica , Animais , Composição de Bases , Códon , Genes Mitocondriais , Loci Gênicos , Genômica/métodos , Dados de Sequência Molecular , Fases de Leitura AbertaRESUMO
The complete mitochondrial genome sequence of Solen grandis that lives in sub-tidal waters and being buried in muddy to fine sand substrates, is described in this paper. The mitogenome (16,794 bp) consists of 12 protein-coding genes (loss of ATPase subunit 8), 22 tRNA genes, 2 rRNA genes and 1 putative control region. It is the typical bivalve mitochondrial gene composition.
Assuntos
Bivalves/genética , Genoma Mitocondrial/genética , Animais , Bivalves/classificação , Genes de RNAr/genética , Região de Controle de Locus Gênico/genética , Dados de Sequência Molecular , Proteínas/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
The complete mitochondrial genome of Paraoncidium reevesii was firstly determined in this paper. The mitogenome (13,842 bp) consists of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes, and 1 putative control region (CR). CR is not well characterized due to lack of discrete conserved sequence blocks. This characteristic is similar with CRs of other invertebrate mitochondrial genomes.
Assuntos
Gastrópodes/genética , Genoma Mitocondrial , Animais , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genéticaRESUMO
As a nutritious mollusk living in the intertidal zone, Onchidiidae has a significant value for biological research. Nine populations were collected from the costal areas such as Chongming (Shanghai), Ningde (Fujian), Haikou (Hainan) in this study. Through the study of anatomy and other methods to investigate the major category characteristics, the nine populations were preliminary classified. By sequencing segments of 18S rRNA gene and constructing phylogenetic trees using these nine populations and four other 18S rRNA gene sequences from GenBank, with Siphonaria as an outgroup, we analyzed the phylogeny of Onchidiidae in mainland China. The trees were used for discussing the relationship between the populations of the Onchidiidae in this study. The results showed that the southern coast has more populations than the northern coast in China. Besides two known species, Onchidium struma (JZH) and O. verruculatum (FJZ & HNZ), five new recorded species may exist: Onchidium(1), Platevindex(2), one Peronia(1) and Paraoncidium(1). The phylogenetic trees indicated that the nine populations can be divided into four subgroups, Peronia, Onchidium, Platevindex and Paraoncidium, and Peronia has the highest confidence level in clustering. Onchidium verruculatum (FJZ & HNZ) should be Peronia verruculata.