OsmiR319-OsPCF5 modulate resistance to brown planthopper in rice through association with MYB proteins.

BACKGROUND: The brown planthopper (BPH) is a kind of piercing-sucking insect specific to rice, with the damage tops the list of pathogens and insects in recent years. microRNAs (miRNAs) are pivotal regulators of plant-environment interactions, while the mechanism underlying their function against insects is largely unknown. RESULTS: Here, we confirmed that OsmiR319, an ancient and conserved miRNA, negatively regulated resistance to BPHs, with overexpression of OsmiR319 susceptible to BPH, while suppression of OsmiR319 resistant to BPH in comparison with wild type. Meanwhile, we identified several targets of OsmiR319 that may mediate BPH resistance. Among them, OsPCF5 was the most obviously induced by BPH feeding, and over expression of OsPCF5 was resistance to BPH. In addition, various biochemical assays verified that OsPCF5 interacted with several MYB proteins, such as OsMYB22, OsMYB30, and OsMYB30C.Genetically, we revealed that both OsMYB22 and OsMYB30C positively regulated BPH resistance. Genetic interaction analyses confirmed that OsMYB22 and OsMYB30C both function in the same genetic pathway with OsmiR319b to mediate BPH resistance. CONCLUSIONS: Altogether, we revealed that OsPCF5 regulates BPH resistance via association with several MYB proteins downstream of OsmiR319, these MYB proteins might function as regulators of BPH resistance through regulating the phenylpropane synthesis.

Impact of endoscopic surveillance on the early diagnosis and endoscopic resection likelihood of gastric cancer.

BACKGROUND: Endoscopy could help detect early gastric cancer (EGC) and improve the prognosis of patients. The aim of this study was to analyze the impact of endoscopy and endoscopic surveillance on the early detection of gastric cancer (GC), GC staging, and treatment selection. METHODS: Patients with GC diagnosed at our center from 2010 to 2022 were retrospectively analyzed and allocated to the short-interval group (had received endoscopy within 3 years before diagnosis), the long-interval group (had received endoscopy more than 3 years before diagnosis), and the unchecked group (had not received endoscopy before diagnosis). The differences in GC staging and treatment modalities among the three groups were analyzed, and the differences in the clinical and pathological features of EGC were further analyzed. RESULTS: One thousand and twenty-five GC patients were included, with 395 cases of EGC and 630 cases of advanced GC. The proportions of EGC in the short-interval, long-interval, and unchecked groups were 98.0%, 84.2%, and 29.8%, respectively (p < 0.001). Among the 387 lesions of 367 EGC patients were resected by endoscopic submucosal dissection (ESD), 341 (88.1%) exhibited curative resection, and 46 (11.9%) involved noncurative resections. Lesions of EGC differed significantly in diameter, depth of invasion, and curative resection rate (p = 0.033, 0.019, and 0.005, respectively). In the short-interval group, 87.8% of the lesions were ≤ 2 cm, 95.6% of the invasion depths were confined to the mucosal layer, and 96.7% of the eCura scores were A or B. Compared with the unchecked group, they had smaller diameters (RR = 0.419, 95% CI 0.234-0.752), shallower invasion depths (RR = 0.286, 95% CI 0.105-0.777), and a higher curative resection rate (RR = 0.215, 95% CI 0.068-0.676). CONCLUSION: Endoscopic surveillance at 3-year intervals can help detect EGC, and the EGC lesions found have smaller diameters and shallower depths of invasion, helping improve the curative resection rate of ESD.

A novel transcriptional repressor complex MYB22-TOPLESS-HDAC1 promotes rice resistance to brown planthopper by repressing F3'H expression.

The brown planthopper (BPH) is the most destructive pest of rice. The MYB transcription factors are vital for rice immunity, but most are activators. Although MYB22 positively regulates rice resistance to BPH and has an EAR motif associated with active repression, it remains unclear whether it is a transcriptional repressor affecting rice-BPH interaction. Genetic analyses revealed that MYB22 regulates rice resistance to BPH via its EAR motif. Several biochemical experiments (e.g. transient transcription assay, Y2H, LCA, and BiFC) indicated that MYB22 is a transcriptional repressor that interacts with the corepressor TOPLESS via its EAR motif and recruits HDAC1 to form a tripartite complex. Flavonoid-3'-hydroxylase (F3'H) is a flavonoid biosynthesis pathway-related gene that negatively regulates rice resistance to BPH. Based on a bioinformatics analysis and the results of EMSA and transient transcription assays, MYB22 can bind directly to the F3'H promoter and repress gene expression along with TOPLESS and HDAC1. We revealed a transcriptional regulatory mechanism influencing the rice-BPH interaction that differs from previously reported mechanisms. Specifically, MYB22-TOPLESS-HDAC1 is a novel transcriptional repressor complex with components that synergistically and positively regulate rice resistance to BPH through the transcriptional repression of F3'H.

USP18 reduces paclitaxol sensitivity of triple-negative breast cancer via autophagy.

Paclitaxol is a first-line treatment for triple-negative breast cancer (TNBC). The molecular mechanisms underlying paclitaxol resistance in TNBC remain largely unclear. In this study, differential expressed genes (DEGs) between TNBC cells and paclitaxol-resistant (taxol-R) TNBC cells were screened by bioinformatics analysis. Among these DEGs, USP18 mRNA expression was significantly increased in taxol-R TNBC cells. USP18 overexpression reduced paclitaxol sensitivity by decreasing paclitaxol-induced apoptosis and cell cycle arrest in TNBC cells. In contrast, USP18 knockdown increased paclitaxol mediated anticancer activity in taxol-R TNBC cells in vitro and in vivo. Mechanistically, USP18 induced autophagy, an important pathway in chemotherapy resistance. The autophagy inhibitor leupeptin could effectively reverse the effect of USP18 on paclitaxol resistance phenotype. These findings suggested that USP18 may be a promising target for overcoming paclitaxol resistance in TNBC.

Competitive Uptake of Dimethylamine and Trimethylamine against Ammonia on Acidic Particles in Marine Atmospheres.

Alkaline gases such as NH3 and amines play important roles in neutralizing acidic particles in the atmosphere. Here, two common gaseous amines (dimethylamine (DMA) and trimethylamine (TMA)), NH3, and their corresponding ions in PM2.5 were measured semicontinuously using an ambient ion monitor-ion chromatography (AIM-IC) system in marine air during a round-trip cruise of approximately 4000 km along the coastline of eastern China. The concentrations of particulate DMA, detected as DMAH+, varied from <4 to 100 ng m-3 and generally decreased with increasing atmospheric NH3 concentrations. Combining observations with thermodynamic equilibrium calculations using the extended aerosol inorganics model (E-AIM) indicated that the competitive uptake of DMA against NH3 on acidic aerosols generally followed thermodynamic equilibria and appeared to be sensitive to DMA/NH3 molar ratios, resulting in molar ratios of DMAH+ to DMA + DMAH+ of 0.31 ± 0.16 (average ± standard deviation) at atmospheric NH3 concentrations over 1.8 µg m-3 (with a corresponding DMA/NH3 ratio of (1.8 ± 1.0) × 10-3), 0.80 ± 0.15 at atmospheric NH3 concentrations below 0.3 µg m-3 (with a corresponding DMA/NH3 ratio of (1.3 ± 0.6) × 10-2), and 0.56 ± 0.19 in the remaining cases. Particulate TMA concentrations, detected as TMAH+, ranged from <2 to 21 ng m-3 and decreased with increasing concentrations of atmospheric NH3. However, TMAH+ was depleted concurrently with the formation of NH4NO3 under low concentrations of atmospheric NH3, contradictory to the calculated increase in the equilibrated concentration of TMAH+ by the E-AIM.

Chemokine (C-C motif) receptor 2 is associated with the pathological grade and inflammatory response in IgAN children.

BACKGROUND: Chemokine (C-C motif) receptor 2 (CCR2) is involved in important physiological and pathological processes, such as inflammation and autoimmune diseases. Abnormal immune and inflammatory responses play a critical role in the development and progression of IgA nephritis (IgAN). However, the role of CCR2 in IgAN is unknown. METHODS: Fifteen IgAN children who were diagnosed by kidney biopsy provided kidney biopsy tissue, blood and urine samples, and age-matched healthy control subjects (blood donators n = 12; tissue donators n = 8) were included. Immunohistochemical analysis was used to detect the expression of CCR2, MCP-1, IL-6, IL-17, and TNF-α in the kidney tissues. Relative optical density (OD) was calculated by Image J software, and the correlation between CCR2 expression and pathological grade in IgAN children was analyzed. RESULTS: The expression of CCR2 significantly increased in mesangial cells of children with IgAN compared to that in control group (P < 0.001), especially in IgAN patients with Lee's grade III to IV (P < 0.001). Interestingly, CCR2 expression was positively correlated with Lee's grade (r = 0.9152, P = 0.0001) in IgAN children. The expression levels of inflammatory factors were markedly increased in IgAN children, and importantly CCR2 expression was positively correlated with it's expression level. CONCLUSIONS: The results suggest that CCR2 signaling might be involved in pathological process and inflammatory responses of children IgAN, and could potentially be an intervention target in children IgAN.

Liquid ToF-SIMS revealing the oil, water, and surfactant interface evolution.

Bilge water from ships is regarded as a major pollutant in the marine environment. Bilge water exists in a stable oil-in-water (O/W) emulsion form. However, little is known about the O/W liquid-liquid (l-l) interface. Traditional bulk characterization approaches are not capable of capturing the chemical changes at the O/W l-l interface. Although surfactants are deemed essential in droplet formation, their roles in bilge water stabilization have not been fully revealed. We have utilized novel in situ chemical imaging tools including in situ scanning electron microscopy (SEM) and in situ time-of-flight secondary ion mass spectrometry (ToF-SIMS) to study the evolving O/W interface using a NAVY bilge model for the first time. The droplet size distribution (DSD) does not change significantly without the addition of X-100 surfactants under static or rocking conditions. Both the oil components and the water clusters are shown to evolve over time at the O/W droplet interface by in situ liquid SIMS imaging. Of particular interest to droplet stabilization, the contribution of surfactants to the aged bilge droplets becomes more significant as the droplet size increases. The higher mass surfactant component does not appear on the droplet surface immediately while many lower mass surfactants are solvated inside the droplet. We have provided the first three-dimensional images of the evolving O/W interface and demonstrated that in situ surface chemical mapping is powerful enough to reveal the complex and dynamic l-l interface in the liquid state. Our observational insights suggest that surfactants are important in mediating droplet growth and facilitating effective separation of bilge water emulsion.

Assessment of ozone variations and meteorological influences at a rural site in Northern Xinjiang.

Ozone concentrations and meteorological data were continuously monitored online from June to December 2013 at the Akedala regional atmosphere station in an arid region of Central Asia. We present daily, monthly, and seasonal variations of ozone concentrations in the atmosphere and elucidate possible emission sources. The ozone concentrations of this region varied from 14.7 to 58.6 ppb. A remarkable seasonal variation of ozone in aerosols was observed with highest level in summer, followed by autumn and winter. The daily peak value of ozone was observed at 9:00-11:00 a.m. while the lowest was at 17:00-19:00 p.m. The backward trajectories of air masses showed potential emission sources to be from the northwest and south during the measurement period. The backward trajectory also revealed that ozone concentrations during the measurement period were likely attributable to the emission from anthropogenic activities, and medium-range atmospheric transport from cities in central Asia and the northern slope of the Tian Shan Mountains.

ToF-SIMS evaluation of PEG-related mass peaks and applications in PEG detection in cosmetic products.

Polyethylene glycols (PEGs) are used in industrial, medical, health care, and personal care applications. The cycling and disposal of synthetic polymers like PEGs pose significant environmental concerns. Detecting and monitoring PEGs in the real world calls for immediate attention. This study unveils the efficacy of time-of-flight secondary ion mass spectrometry (ToF-SIMS) as a reliable approach for precise analysis and identification of reference PEGs and PEGs used in cosmetic products. By comparing SIMS spectra, we show remarkable sensitivity in pinpointing distinctive ion peaks inherent to various PEG compounds. Moreover, the employment of principal component analysis effectively discriminates compositions among different samples. Notably, the application of SIMS two-dimensional image analysis visually portrays the spatial distribution of various PEGs as reference materials. The same is observed in authentic cosmetic products. The application of ToF-SIMS underscores its potential in distinguishing PEGs within intricate environmental context. ToF-SIMS provides an effective solution to studying emerging environmental challenges, offering straightforward sample preparation and superior detection of synthetic organics in mass spectral analysis. These features show that SIMS can serve as a promising alternative for evaluation and assessment of PEGs in terms of the source, emission, and transport of anthropogenic organics.

Secondary Ion Mass Spectral Imaging of Metals and Alloys.

Secondary Ion Mass Spectrometry (SIMS) is an outstanding technique for Mass Spectral Imaging (MSI) due to its notable advantages, including high sensitivity, selectivity, and high dynamic range. As a result, SIMS has been employed across many domains of science. In this review, we provide an in-depth overview of the fundamental principles underlying SIMS, followed by an account of the recent development of SIMS instruments. The review encompasses various applications of specific SIMS instruments, notably static SIMS with time-of-flight SIMS (ToF-SIMS) as a widely used platform and dynamic SIMS with Nano SIMS and large geometry SIMS as successful instruments. We particularly focus on SIMS utility in microanalysis and imaging of metals and alloys as materials of interest. Additionally, we discuss the challenges in big SIMS data analysis and give examples of machine leaning (ML) and Artificial Intelligence (AI) for effective MSI data analysis. Finally, we recommend the outlook of SIMS development. It is anticipated that in situ and operando SIMS has the potential to significantly enhance the investigation of metals and alloys by enabling real-time examinations of material surfaces and interfaces during dynamic transformations.

Magnaporthe oryzae infection triggers rice resistance to brown planthopper through the influence of jasmonic acid on the flavonoid biosynthesis pathway.

In agroecosystems, plants are constantly exposed to attack from diverse herbivorous insects and microbes, and infestation with one species may change the plant defense response to other species. In our investigation of the relationships among rice plants, the brown planthopper Nilaparvata lugens (Stål) and the rice blast fungus Magnaporthe oryzae, we observed a significant increase in the resistance of rice treated with rice blast to N. lugens, as evidenced by improved plant survival rates in a small population resistance study. Subsequent transcriptome data analysis revealed that the rice blast fungus can induce the expression of genes in the jasmonic acid (JA) and flavonoid pathways. Similar to the flavonoid pathway, the JA pathway also contains 2 types of genes that exhibit similar and opposite trends in response to N. lugens and rice blast. Among these genes, the osjaz1 mutant and the osmyc2 mutant were phenotypically confirmed to positively and negatively regulate rice resistance to N. lugens and rice blast, respectively. Subsequent mass spectrometry and quantification experiments showed that the exogenous application of methyl jasmonate (MeJA) can induce the accumulation of eriodictyol, naringenin and quercetin, as well as the expression of OsF3H, Os4CL5 and OsCHI in the flavonoid pathway. This suggests a close connection between the JA pathway and the flavonoid pathway. However, OsF3'H, which negatively regulates rice resistance to N. lugens and rice blast, did not show increased expression. Phenotypic and molecular experiments confirmed that OsMYC2 can bind to and inhibit the expression of OsF3'H, thus revealing the mechanism of rice resistance to N. lugens after treatment with rice blast. These findings will deepen our understanding of the interactions among rice, N. lugens and rice blast.

OsmiR159 Modulate BPH Resistance Through Regulating G-Protein γ Subunit GS3 Gene in Rice.

Brown planthopper (BPH) is the most destructive insect pest to rice that causes tremendous yield loss each year in rice planting Asia and South-East Asia areas. Compared with traditional chemical-based treatment, utilization of plant endogenous resistance is a more effective and environmental-friendly way for BPH control. Accordingly, quite a few quantitative trait loci (QTLs) for BPH resistance were cloned using forward genetics. However, BPH is apt to change quickly into new biotypes to overcome plant resistance, therefore, new resistance resources and genes are continuously needed. miRNAs are important regulators in both plant development and physiological regulation including immunity, and might be used as effective supplements for BPH resistance QTLs. miR159 is an ancient and conserved miRNA. In this study, we found that each OsMIR159 gene in rice responded to BPH feeding very obviously, and genetic function assay proved them to negatively regulate BPH resistance, with STTM159 showing resistance to BPH, and over expression of OsmiR159d susceptible to BPH. One target genes of OsmiR159, OsGAMYBL2, positively regulated BPH resistance. Further biochemical studies revealed that OsGAMYBL2 could directly bind to the promoter of G-protein γ subunit encoding GS3 gene and repress its expression. And genetically, GS3 responded to BPH feeding promptly and negatively regulated BPH resistance, GS3 over expression plants were susceptible to BPH, while GS3 knock-out plants were resistant to BPH. Thus, we identified new function of OsmiR159-OsGAMYBL2 in mediating BPH response, and revealed a new OsmiR159-G protein pathway that mediates BPH resistance in rice.

The occurrence of lower-than-expected bulk NCCN values over the marginal seas of China - Implications for competitive activation of marine aerosols.

In this study, we combined the measured bulk particle number concentration (NCN), particle number size distribution (PNSD) and bulk cloud condensation nuclei concentration (NCCN) at various supersaturation (SS) levels to investigate competitive activation of aerosols in the marine atmospheres over the marginal seas of China during two winter campaigns Campaign A (December 9-19, 2019) and Campaign B (December 28, 2019-January 16, 2020). During the two campaigns, we observed various categories of aerosols, i.e., long-range transport continental aerosols, clean marine aerosols, grown new particles ranging from nucleation mode to larger sizes, and grown pre-existing particles ranging from Aitken mode to accumulation mode size, etc. We found that the measured NCCN increased by only approximately 30 % with increases in SS levels from 0.2 % to 1.0 %, e.g., (1.8 ± 1.4) × 103 cm-3 at SS = 0.2 % and (2.4 ± 1.4) × 103 cm-3 at SS = 1.0 % during Campaign A. We further calculated the hygroscopicity parameter kappa (κ) by combining simultaneously measured PNSD and bulk NCCN to explore the causes. The calculated κ values were below 0.1 at SS = 0.4 % during the 72 % (or 88 %) period of Campaign A (or Campaign B). When κ values below 0.1 (or 0.2) were excluded, the remaining κ values were apparently reasonable, with an average of 0.22 (or 0.36) and a standard deviation of 0.10 (or 0.21) at SS = 0.4 % during Campaign A (or Campaign B). The unexpectedly lower κ values were discussed in terms of competitive activation of aerosols in marine atmospheres together with its net contribution to lowering the measured bulk NCCN below the expected value.

MCPIP1 alleviated alcohol-induced immune dysfunction via the MAPK/ERK signaling pathway.

RATIONALE: In recent years, monocyte chemotactic protein-induced protein 1 (MCPIP1) has been reported to control inflammation via IL-10. OBJECTIVES: The aims of this study were to determine (1) whether MCPIP1 can repair damage to the immune system after alcohol use and (2) whether MCPIP1 can repair the immune function impaired by alcohol use through the MAPK/ERK signaling pathway. Our results will inform the treatment of immune dysfunction caused by alcohol consumption. METHODS: Scrambled shRNA or MCPIP-1-shRNA carried by the lentiviral vector (50µl each at 1×108TU/ml) was injected retrogradely through the pancreatic duct to pretreat male C57BL/6 mice. Five days after the injection, mice were exposed to intragastric ethanol infusion (5g/kg, 25% ethanol w/v) daily or vehicle for 10 days. RESULTS: MCPIP-1 protein was increased in the pancreas after alcohol exposure. MCPIP-1 shRNA specifically decreased MCPIP-1 protein expression and mRNA level in the pancreas. Specific knockdown of MCPIP-1 exacerbates pancreatic necrosis, interstitial edema, and inflammatory infiltrates after alcohol exposure. Meanwhile, specific knockdown of MCPIP-1 also increased pancreatic pro-inflammatory cytokine (IL-6 and IL-1ß), chemokine MCP-1, and chemokine receptor 2 (CCR2) after alcohol exposure. What's more, p-JNK and p-ERK in the pancreas were all similarly increased in response to pancreas-specific knockdown of MCPIP-1 during alcohol exposure. CONCLUSIONS: Taken together, the results above suggested that MCPIP1 repairs the immune function impaired by alcohol use via stimulating JNK and ERK pathways. Our results will inform the treatment of immune dysfunction caused by alcohol consumption.

A clear north-to-south spatial gradience of chloride in marine aerosol in Chinese seas under the influence of East Asian Winter Monsoon.

Particulate chloride is a major component of sea salt particles and plays a key role in atmospheric chemistry. Anthropogenic pollutants over the northeastern Asia can be transported to the adjacent seas through the northwest monsoon, which profoundly influences the chloride chemistry over the seas. In this study, spatial distribution of particulate chloride and its sources over the Chinese seas were investigated based on shipboard particle samplings especially online Single Particle Aerosol Mass Spectrometer (SPAMS) over Bohai Sea, North Yellow Sea, and South Yellow Sea (SYS) during a cruise in November 2012. A strong north-to-south (N-S) gradience in marine aerosol composition was found. The Cl-/Na+ ratios in PM2.5 and single particle composition by SPAMS indicated remarkable chloride enrichment in marine aerosol in the north (Bohai Sea), while depletion in southern SYS. The results of size distribution showed that particulate chloride had higher concentration in coarse particles, while the Cl-/Na+ ratio was much higher in submicron particles. In the north (38-40°N), biomass burning, carbonaceous, and Pb-rich type particles had high fractions in all chloride-containing particles identified by SPAMS (on average 66%). Combining chemical composition with back trajectory, it was found that fine-mode chloride enrichment in the north was mainly due to anthropogenic emission especially coal combustion and biomass burning from northern China. However, the high fine-mode chloride depletion in the south (32-34°N) was probably due to acid replacement by sulfate in aged aerosol during atmospheric transport. Our new findings reveal that marine aerosol in Chinese seas would show a clear N-S pattern of more fresh and anthropogenic enriched particles in the north, but more aged aerosol in the south during the East Asia Winter Monsoon, which provides new insights for the quantitative assessment of anthropogenic impact on marine aerosol and future modeling study.

Evolutionary characterization of miR396s in Poaceae exemplified by their genetic effects in wheat and maize.

MiR396s play important roles in regulating plant growth and stress response, and great potential for crop yield promotion was anticipated. For more comprehensive and precise understanding of miR396s in Poaceae, we analyzed the phylogenetic linkage, gene expression, and chromosomal distribution of miR396s in this study. Although the mature miR396s' sequences were mostly conserved, differential expression patterns and chromosomal distribution were found among Poaceae species including the major cereal crops rice, wheat, and maize. Consistently, in comparison with rice, wheat and maize plants transformed with the target mimicry construct of miR396 (MIM396) exhibited differential effects on grain size and disease resistance. While the TaMIM396 plants showed increased grain size, panicle length and sensitivity to B. graminis, the ZmMIM396 plants didn't show obvious changes in grain size and disease resistance. In Addition, several GROWTH-REGULATING FACTOR (GRF) genes in wheat and maize were repressed by miR396s, which could be reversed by MIM396, confirming the conserved regulatory roles of miR396 on GRFs. While providing new solution to enhance grain yield in wheat and revealing potential regulatory variations of miR396s in controlling grain size and disease resistance in different crops, this study gives clues to further explore miR396s' functions in other Poaceae species.

Lateral Supraorbital Approach for the Surgical Clipping of Ruptured Tiny Anterior Circulation Aneurysms.

OBJECTIVE: To evaluate the efficacy and usefulness of clipping procedure by lateral supraorbital approach for tiny anterior circulation aneurysms. STUDY DESIGN: An observational study. PLACE AND DURATION OF STUDY: Department of Neurosurgery, The Second Hospital of Anhui Medical University, Hefei, Anhui Province, China, from January 2013 to March 2018. METHODOLOGY: Thirty-eight patients who had suffered aneurysm rupture, resulting in subarachnoid hemorrhage, were selected. Fourty-four very small aneurysms with a maximum diameter of ≤3 mm that presented different configurations, were identified using digital subtraction angiography (DSA). Clinical data of patients, including clinical information, microsurgery procedure, procedural complications, clinical outcomes, and follow-up were analysed. RESULTS: All ruptured aneurysms involved the anterior circulation, located in middle cerebral artery (19/38), anterior communicating artery (10/38), posterior communicating artery (6/38), anterior choroidal artery (2/38), and ophthalmic artery (1/38). Complete occlusions were achieved in all of the lesions by surgical clipping via the lateral supraorbital approach. There were no intra-procedural ruptures or procedure-related vasospasm, epilepsy, hydrocephalus. Glasgow outcome scale (GOS) scores showed that 34 patient (86.84%) had good outcomes (GOS 4-5) at the time of discharge. There was no residual neck or recurrence following computed tomography angiography (CTA) and DSA at follow-up in 20 cases. CONCLUSION: Surgical clipping via lateral supraorbital approach presented a low rate of adverse events related to the procedure, and was safe and effective in ruptured tiny aneurysms. Key Words: Tiny aneurysm; Surgical clipping; Clinical outcome; Lateral supraorbital approach.

Sources and formation of nucleation mode particles in remote tropical marine atmospheres over the South China Sea and the Northwest Pacific Ocean.

A fast mobility particle sizer operating at a one-second time resolution was used to measure aerosol particle number size distribution (5.6-560 nm) in marine conditions over the South China Sea (SCS) from 29 March to 2 May 2017 and in the tropic zone of the Northwest Pacific Ocean (NWPO) from 10 to 29 October 2018. The clean background number concentration of nucleation mode atmospheric particles (<30 nm) was approximately 0.6 × 103 cm-3 in these areas. Two nighttime and five daytime strong new particle formation (NPF) events were observed to occur extending over a spatial scale from 2 to 140 km in the SCS, with a net increase of nucleation mode particles of 4.5 × 104 cm-3 ± 3.4 × 104 cm-3 during five of the seven events. Nighttime NPF events were unlikely associated with sulfuric acid vapor because of lack of photochemical reactions. Daytime NPF events share several common features with nighttime NPF events, e.g., dramatic spatiotemporal variations in the number concentration of the nucleation mode particles. Without aerosol precursor measurements we cannot address the vapors driving the formation process. However, our results show no banana-shaped growth of the particles. The growth into larger particle sizes seems to be restricted by the availability of condensable components in the gas phase. The nucleation mode was observed and sometimes even dominated the number concentration over other particle modes in the marine atmosphere over the tropic zone of the NWPO. In addition, more data obtained during the two campaigns and other campaigns were also applied to strengthen the analysis in terms of origins, formation and absent growth of nucleation mode particles in the marine atmospheres over the two tropic zones.

Surface evolution of synthetic bilgewater emulsion.

Bilgewater is a regulated shipboard produced waste stream that often contains oil-in-water emulsion. Fundamental knowledge of emulsion surface changes is required for improved wastewater treatment; however, limited information is currently available. We have reported the first surface characterization of synthetic bilgewater emulsions using time-of-flight secondary ion mass spectrometry (ToF-SIMS) coupled with optical microscopy. A Navy standard bilgewater solution consisting of a hydrocarbon and detergent mixture is used as the synthetic bilgewater emulsion model. Both fresh and aged emulsion samples are analyzed to determine their droplet size distributions (DSDs) and surface chemical composition. Our results show that fresh emulsions are largely mono-modal with hydrocarbon fragments as the main surface composition. Aged emulsions are also mono-modal with slightly larger size. Both SIMS spectral comparison and Principal Component Analysis (PCA) show that some surfactant components appear on the fresh emulsion surface while larger molecular weight components appear at the aged bilge droplet surface. Our results indicate that the oil-water interface evolves after emulsion droplet formation. More importantly, surface evolution not only changes the bilgewater DSD, but also alters the surface chemical composition and reactivity.

Identification of TYR mutations in patients with oculocutaneous albinism.

Oculocutaneous albinism (OCA) is a set of autosomal recessive disorders characterized by hypopigmented hair, skin and eyes. Homozygous or compound heterozygous mutations in the tyrosinase (TYR) gene can cause OCA1, which is the most common and severe subtype of albinism. In the present study, 17 patients with non­syndromic OCA were enrolled from eight provinces of China and were non­consanguineous, with the exception of Patient 4000301. Total genomic DNA was isolated from peripheral blood. Screening was performed for the whole exons and their flanking regions of the TYR gene using Sanger sequencing and the pathogenicity of variants was predicted using in silico analysis. In total, 12 TYR mutations were identified in 10 patients, respectively. Of these, two patients carried homozygous mutations and eight patients carried compound heterozygous mutations. Among the 12 TYR mutations, two missense mutations c.1198T>G (p.W400G) and c.819G>T (p.Q273H) were novel. The results of the present study expand the mutation spectrum of the TYR gene, which may further assist in the prenatal examination and genetic diagnosis of OCA.