RESUMO
Solid-phase microextraction (SPME) coupled with electrospray ionization mass spectrometry (ESI-MS) was developed for rapid and sensitive determination of endogenous androgens. The SPME probe is coated with covalent organic frameworks (COFs) synthesized by reacting 1,3,5-tri(4-aminophenyl)benzene (TPB) with 2,5-dioctyloxybenzaldehyde (C8PDA). This COFs-SPME probe offers several advantages, including enhanced extraction efficiency and stability. The analytical method exhibited wide linearity (0.1-100.0 µg L-1), low limits of detection (0.03-0.07 µg L-1), high enrichment factors (37-154), and satisfactory relative standard deviations (RSDs) for both within one probe (4.0-14.8%) and between different probes (3.4-12.7%). These remarkable performance characteristics highlight the reliability and precision of the COFs-SPME-ESI-MS method. The developed method was successfully applied to detect five kinds of endogenous androgens in female serum samples, indicating that the developed analytical method has great potential for application in preliminary clinical diagnosis.
Assuntos
Androgênios , Limite de Detecção , Microextração em Fase Sólida , Espectrometria de Massas por Ionização por Electrospray , Microextração em Fase Sólida/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Humanos , Androgênios/sangue , Androgênios/análise , Androgênios/química , Feminino , Estruturas Metalorgânicas/química , Reprodutibilidade dos TestesRESUMO
Glial fibrillary acidic protein (GFAP), an intracellular type III intermediate filament protein, provides structural support and maintains the mechanical integrity of astrocytes. It is predominantly found in the astrocytes which are the most abundant subtypes of glial cells in the brain and spinal cord. As a marker protein of astrocytes, GFAP may exert a variety of physiological effects in neurological diseases. For example, previous published literatures showed that autoimmune GFAP astrocytopathy is an inflammatory disease of the central nervous system (CNS). Moreover, the studies of GFAP in brain tumors mainly focus on the predictive value of tumor volume. Furthermore, using biomarkers in the early setting will lead to a simplified and standardized way to estimate the poor outcome in traumatic brain injury (TBI) and ischemic stroke. Recently, observational studies revealed that cerebrospinal fluid (CSF) GFAP, as a valuable potential diagnostic biomarker for neurosyphilis, had a sensitivity of 76.60% and specificity of 85.56%. The reason plasma GFAP could serve as a promising biomarker for diagnosis and prediction of Alzheimer's disease (AD) is that it effectively distinguished AD dementia from multiple neurodegenerative diseases and predicted the individual risk of AD progression. In addition, GFAP can be helpful in differentiating relapsing-remitting multiple sclerosis (RRMS) versus progressive MS (PMS). This review article aims to provide an overview of GFAP in the prediction of clinical progression in neuroinflammation, brain tumors, TBI, ischemic stroke, genetic disorders, neurodegeneration and other diseases in the CNS and to explore the potential therapeutic methods.
Assuntos
Neoplasias Encefálicas , AVC Isquêmico , Humanos , Proteína Glial Fibrilar Ácida , Filamentos Intermediários/metabolismo , Biomarcadores , Progressão da DoençaRESUMO
Sunitinib, N-desmethyl imatinib, dasatinib, imatinib, and bosutinib are tyrosine kinase inhibitors (TKIs) that are commonly employed in the treatment of a multitude of cancers. However, the inappropriate concentrations of TKIs can result in ineffective treatment or the emergence of multiple adverse effects. Consequently, the development of a rapid and sensitive analytical method for TKIs is of paramount importance for the safe administration of drugs. In this work, solid-phase microextraction (SPME) probe combined with an electrospray ionization mass spectrometry (ESI-MS) coupling platform was constructed for rapid and sensitive determination of TKIs. The covalent organic frameworks (COFs) coated SPME probe was made of 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) and 2,5-dibutoxyterephthalaldehyde (DBTA) by in-situ layer-by-layer chemical bonding synthesis strategy. The TAPT-DBTA-SPME probe exhibited several advantageous properties which rendered it suitable for the enrichment of TKIs. Under the optimal conditions, the developed analytical method demonstrated a broad linear range (0.05-500.00 µg/L), a low limit of detection (0.02 µg/L) and a high enrichment factor (51-203) for TKIs. The developed analytical method was successfully applied to a pharmacokinetic study of TKIs in mouse plasma and tissue matrix, demonstrating that the proposed analytical method has promise for clinical applications and metabolic monitoring.
Assuntos
Limite de Detecção , Inibidores de Proteínas Quinases , Microextração em Fase Sólida , Espectrometria de Massas por Ionização por Electrospray , Microextração em Fase Sólida/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , Camundongos , Inibidores de Proteínas Quinases/análise , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/sangue , Estruturas Metalorgânicas/química , Aço Inoxidável/química , Triazinas/análise , Triazinas/química , Triazinas/sangue , Reprodutibilidade dos TestesRESUMO
Soil macro-aggregates are the main location for soil organic carbon ï¼SOCï¼ sequestration, which is of great significance to improve soil fertility. This study aimed to understand the mechanisms of the organic carbon ï¼OCï¼ sequestration in macroaggregates and improve crop yield in wheat fields on the loess plateau. With the aggregate-density fractionation method, an eight-year experiment was conducted to investigate the following three factorsï¼ â the effects of long-term fertilization on OC fractions within macroaggregatesï¼ â¡ the variation characteristics of OC fractions within macroaggregates, including coarse particulate organic carbon ï¼cPOCï¼, fine particulate organic carbon ï¼fPOCï¼, intra-microaggregate particulate organic carbon ï¼iPOCï¼, free silt and clay particulate carbon ï¼s+c_fï¼, and intra-microaggregate silt and clay particulate carbon ï¼s+c_mï¼ï¼ ⢠and the relationships between them and SOC input and yield formation. The treatments included no fertilization ï¼CKï¼, farmer pattern ï¼NPï¼, optimized fertilizers pattern ï¼NPKï¼, optimized fertilizers + organic fertilizers pattern ï¼NPKMï¼, and optimized fertilizers + biological organic fertilizers pattern ï¼NPKBï¼. The results showed that the application of organic and chemical fertilizer ï¼NPKM and NPKBï¼ improved significantly the SOC content in macroaggregates compared with that in the single fertilizer treatment ï¼NP and NPKï¼, which had a greater increase in SOC content in macroaggregates than that of the soil. All fertilization treatments had a tendency to increase the content of fractions iPOC, fPOC, and iPOC in macroaggregates, but silt and clay carbon ï¼s+c_f and s+c_mï¼ contents were decreased. The application of manure combined with chemicals markedly increased the allocations of fractions cPOC, fPOC, and iPOC reserves, but it greatly decreased ï¼s+c_fï¼ reserves allocation. However, the application of chemical fertilizers only significantly increased the proportion of cPOC reserves in macroaggregates. Correlation analysis showed that there were significant positive correlations among wheat grain yield and OC fractions ï¼cPOC and fPOCï¼ contents, SOC content, the OC content of >0.25 mm macroaggregates, and SOC input, and the correlation coefficient was 0.645-0.883. In conclusion, long-term fertilization, especially combined with organic fertilizer, could promote the free silt and clay carbon fraction ï¼s+c_fï¼ to transfer into other forms of OC components through the increase in soil carbon input in the wheat field of the loess plateau. Furthermore, the OC content of macroaggregates was increased overall, providing a good soil environment for crop yield.