RESUMO
Two non-pigmented strains in the genus Chromobacterium, MWU14-2602T and MWU13-2610T, were isolated from wild cranberry bogs in the Cape Cod National Seashore, USA. The isolates were characterized by genomic and phenotypic analyses, the results of which indicated that they represent two novel species. Based on total genome sequences, the closest relatives were in the Chromobacterium amazonense group, which includes the recently described Chromobacterium paludis. Whole genome sequences were compared by genome blast distance phylogeny, digital DNA-DNA hybridization and average nucleotide identity analyses with each other and with the type strains of their nearest species. MWU14-2602T and MWU13-2610T fell well below the accepted cutoff values for species relatedness, clearly indicating that they represent novel species. Although little is known about these organisms in situ, under laboratory conditions, MWU13-2610T produced a modest amount of HCN and was strongly positive for exoprotease activity, whereas MWU14-2602T did not produce HCN or exoproteases. The predominant fatty acids for both isolates were summed C16â:â1ω7cis/C16â:â1ω6cis. Both isolates produced siderophores and pyomelanin pigment on rich media, and neither was haemolytic on sheep blood agar. We propose the names Chromobacterium alticapitis sp. nov. (type strain MWU14-2602T=ATCC TSD 260T=CCOS 1979T) and Chromobacterium sinusclupearum sp. nov. (type strain MWU13-2610T=ATCC TSD-259T=CCOS 1981T) for these taxa.
Assuntos
Chromobacterium , Vaccinium macrocarpon , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ovinos , Vaccinium macrocarpon/genética , Áreas AlagadasRESUMO
Published data on the genome sequences of Chromobacterium pseudoviolaceum LMG 3953T and Chromobacterium violaceum ATCC 12472T suggest that both isolates belong to the same species. Previous 16S rRNA gene sequence comparisons had demonstrated that these species share 99.9â% sequence similarity. Initial investigations of fatty acid patterns and substrate utilization had shown only a few differences between the type strains of both species. Despite the 47.5â% homology by DNA-DNA hybridization studies between these strains, in silico whole genome sequence comparisons have clearly demonstrated that OrthoANIu and Mash/MinHash values were >99.18â%. Molecular phylogeny based on the estimated phylogenetic positions of the published genome sequences of the two type strains, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses indicate that these strains are members of the same species. Due to priority of publication and validation of names, Chromobacterium pseudoviolaceum is reclassified as Chromobacterium violaceum.
Assuntos
Chromobacterium/classificação , Filogenia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
A large number of Gram-negative, motile, mesophilic, violacein-producing bacteria were isolated from the soils and roots of Vaccinium macrocarpon Ait. and Kalmia angustifolia L. plants and from irrigation ponds associated with wild and cultivated cranberry bogs in Massachusetts, USA. Phylogenetic analyses of 16S rRNA gene sequences placed these isolates in a clade with Chromobacterium species, but the specialized environment from which they were isolated, their low genomic DNA relatedness with Chromobacterium violaceum ATCC 12472(T) and C. subtsugae PRAA4-1(T), significant differences in fatty acid composition and colony morphology indicate that the cranberry and Kalmia isolates comprise a separate species of Chromobacterium, for which the name Chromobacterium vaccinii sp. nov. is proposed. Strain MWU205(T) (â=âATCC BAA-2314(T) â=âDSM 25150(T)) is proposed as the type strain for the novel species. Phenotypic analysis of 26 independent isolates of C. vaccinii sp. nov. indicates that, despite close geographical and biological proximity, there is considerable metabolic diversity among individuals within the population.
Assuntos
Chromobacterium/classificação , Filogenia , Vaccinium macrocarpon/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Chromobacterium/genética , Chromobacterium/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/análise , Indóis/análise , Massachusetts , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Lagoas/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Áreas AlagadasRESUMO
Priestia megaterium MWU16-30321 was isolated from a mixed bacterial culture in a cranberry stem gall in Massachusetts following a severe winter. The genome is 5,623,390 bp in size and putatively encodes indole-3-acetic acid acetyltransferase, a key enzyme in tryptophan-dependent indole-3-acetic acid production.
RESUMO
Pseudomonas sp. strain MWU15-20650 was isolated from wild cranberry fruit surfaces in the Cape Cod National Seashore. The draft genome is 6.2 Mbp, with a G+C content of 59%, and contains predicted genes for type VI secretion systems and an N-acyl-homoserine lactone acylase. The closest known relative is Pseudomonas haemolytica.
RESUMO
Pseudomonas sp. strains MWU12-2037 and MWU12-2345 were isolated from peat and sandy bog soils in wild cranberry bogs in the Cape Cod National Seashore (Massachusetts, USA) as part of a culture-dependent survey of relatively unexplored wetlands soil microbiomes. Both genomes exceeded 7 Mbp.
RESUMO
Pseudomonas spp. are dominant in many soils, but little is known about how they interact with other members of the soil microbiome. Pseudomonas sp. strain MWU13-2516, isolated from a wild cranberry bog in Massachusetts, has predicted genes for hemolysins, usually associated with pathogens, and type 6 secretion systems.
RESUMO
Pseudomonas sp. strain MWU13-3659 was isolated from cultivated cranberry bog soil in Massachusetts, USA. Its closest known relative is Pseudomonas entomophila (digital DNA-DNA hybridization [d4 formula] value of 57.2% and average nucleotide identity based on BLAST value of 93.90), and its genome contains putative gene clusters for the production of polyketides, siderophores, and cyclic lipopeptides that have insecticidal activity in other proteobacteria.
RESUMO
Pseudomonas sp. strains MWU12-2319 and MWU12-2311 were isolated from the soil of a wild cranberry bog in the Cape Cod National Seashore as part of a culture-dependent bacterial population survey. The genomes exceed 7 Mbp and contain putative gene clusters for the biosurfactant orfamides A and C.
RESUMO
Curtobacterium sp. strain MWU13-2055 was isolated from cranberry fruit surfaces in the Cape Cod National Seashore. The genome is 4 Mbp long with a large number of genes predicted to be devoted to heavy metal resistance, including the copAZ operon and translocases for Pb, Cd, Zn, Hg, and Cu.
RESUMO
Pseudomonas sp. strains MWU13-2100 and MWU13-2105 were isolated from a wild cranberry bog with Pipestone loamy coarse sand soil in Truro, Massachusetts, and taxonomically assigned based on whole-genome sequences. The draft genomes are most closely related to P. batumici (41.4% and 41.8% dDDHd4), but with only 50.8 dDDHd4 to each other.
RESUMO
Here, we present the draft genome sequences of Pseudomonas sp. strains MWU12-2020 and MWU12-3103b, isolated from the rhizospheres of wild and cultivated cranberry bogs in southeastern Massachusetts; these strains are unrelated to known Pseudomonas species. The genomes of both isolates exceed 6 Mbp and contain predicted ice nucleation and type VI and III secretion system genes.
RESUMO
Pseudomonas protegens strain MWU12-2233 was isolated from wild cranberry fruit surfaces in Provincetown, MA. The genome contains putative hydrogen cyanide synthase and type VI secretion systems which can act symbiotically on plant health by suppressing competitors, indicating a role in indigenous microfloral disease and insect pest suppression.
RESUMO
Four Pseudomonas spp. and Xanthomonas arboricola were isolated from cranberry stem galls in Carver, MA, and taxonomically assigned at the genus level based on the 16S rRNA sequence and phenotypes. X. arboricola had not been associated previously with cranberry stem galls or any cranberry disease.
RESUMO
Pseudomonas sp. strains MWU12-2029, MWU12-3088, and MWU12-3091 were isolated from wild and cultivated cranberry bog soils in southeastern Massachusetts. The three isolates are closely related to Pseudomonas kribbensis, a not validly published member of the P. fluorescens group, and contain three putative insecticidal protein genes, including the toxin complex A gene (tcaC).
RESUMO
Pseudomonas moorei has been used to detoxify recalcitrant environmental contaminants from the pharmaceutical industry. Two P. moorei strains were isolated from soil in the pristine wild cranberry bogs of the Cape Cod National Seashore that putatively encode genes for degradation of 4- and 5-chlorosalicylates, acetaminophen, and diclofenac.
RESUMO
Chromobacterium sp. strain IRSSSOUMB001 with potent insecticidal activity was isolated from Anopheles gambiae s.l. in Burkina Faso. The draft genome is 5,090,822 bp and encodes predicted genes for hydrogen cyanide production, haemolysin, a T3SS, and yopE, which are potential virulence factors against mosquitoes.
RESUMO
The surfaces of plants are colonized by a rich diversity of microbes but are largely unexplored. Here, we present the draft genome sequences of five Pseudomonas spp. isolated from cultivated cranberry fruit surfaces. Although the isolates represent four different species, their genomes all contain conserved iron sequestration and uptake genes.
RESUMO
Exploration of novel environments such as low-pH wild cranberry bog soils yields a rich diversity of bacteria, including Pseudomonas spp. Here, we present the draft genome sequence of Pseudomonas sp. strain MWU12-2323, isolated from wild cranberry plant rhizosphere. The genome has secondary metabolite genes encoding carbohydrate polymer-degrading enzymes.