Processing bulk natural wood into a high-performance structural material.

Synthetic structural materials with exceptional mechanical performance suffer from either large weight and adverse environmental impact (for example, steels and alloys) or complex manufacturing processes and thus high cost (for example, polymer-based and biomimetic composites). Natural wood is a low-cost and abundant material and has been used for millennia as a structural material for building and furniture construction. However, the mechanical performance of natural wood (its strength and toughness) is unsatisfactory for many advanced engineering structures and applications. Pre-treatment with steam, heat, ammonia or cold rolling followed by densification has led to the enhanced mechanical performance of natural wood. However, the existing methods result in incomplete densification and lack dimensional stability, particularly in response to humid environments, and wood treated in these ways can expand and weaken. Here we report a simple and effective strategy to transform bulk natural wood directly into a high-performance structural material with a more than tenfold increase in strength, toughness and ballistic resistance and with greater dimensional stability. Our two-step process involves the partial removal of lignin and hemicellulose from the natural wood via a boiling process in an aqueous mixture of NaOH and Na2SO3 followed by hot-pressing, leading to the total collapse of cell walls and the complete densification of the natural wood with highly aligned cellulose nanofibres. This strategy is shown to be universally effective for various species of wood. Our processed wood has a specific strength higher than that of most structural metals and alloys, making it a low-cost, high-performance, lightweight alternative.

Selective Chiral Recognition between Amino Acids and Growing Gypsum Crystals.

In nature, selective chiral interactions between biomolecules and minerals provide insight into the mysterious origin of homochirality. Here, we show growing gypsum crystals in a nonequilibrium state can recognize chiral enantiomers of amino acids. The chiral selection for amino acids with different functional groups by growing minerals are distinct. For 11 amino acids, the d-isomer slows dynamic gypsum growth more than the l-isomer, whereas for another 7 amino acids, the opposite was observed. These differences in chiral recognition are attributed to the different stereochemical matching between the chiral amino acids and the dynamic steps of growing gypsum. These stereoselective interactions between amino acid enantiomers and dynamic growing crystals can be applied toward the fabrication of gypsum cements to regulate their structure and mechanical properties. These findings provide insight into understanding the mechanism of the origin of homochirality in nature and suggest a pathway for constructing advanced functional materials.

Cellulose ionic conductors with high differential thermal voltage for low-grade heat harvesting.

Converting low-grade heat into useful electricity requires a technology that is efficient and cost effective. Here, we demonstrate a cellulosic membrane that relies on sub-nanoscale confinement of ions in oxidized and aligned cellulose molecular chains to enhance selective diffusion under a thermal gradient. After infiltrating electrolyte into the cellulosic membrane and applying an axial temperature gradient, the ionic conductor exhibits a thermal gradient ratio (analogous to the Seebeck coefficient in thermoelectrics) of 24 mV K-1-more than twice the highest value reported until now. We attribute the enhanced thermally generated voltage to effective sodium ion insertion into the charged molecular chains of the cellulosic membrane, which consists of type II cellulose, while this process does not occur in natural wood or type I cellulose. With this material, we demonstrate a flexible and biocompatible heat-to-electricity conversion device via nanoscale engineering based on sustainable materials that can enable large-scale manufacture.

High-capacity, low-tortuosity, and channel-guided lithium metal anode.

Lithium metal anode with the highest capacity and lowest anode potential is extremely attractive to battery technologies, but infinite volume change during the Li stripping/plating process results in cracks and fractures of the solid electrolyte interphase, low Coulombic efficiency, and dendritic growth of Li. Here, we use a carbonized wood (C-wood) as a 3D, highly porous (73% porosity) conductive framework with well-aligned channels as Li host material. We discovered that molten Li metal can infuse into the straight channels of C-wood to form a Li/C-wood electrode after surface treatment. The C-wood channels function as excellent guides in which the Li stripping/plating process can take place and effectively confine the volume change that occurs. Moreover, the local current density can be minimized due to the 3D C-wood framework. Therefore, in symmetric cells, the as-prepared Li/C-wood electrode presents a lower overpotential (90 mV at 3 mA⋅cm-2), more-stable stripping/plating profiles, and better cycling performance (∼150 h at 3 mA⋅cm-2) compared with bare Li metal electrode. Our findings may open up a solution for fabricating stable Li metal anode, which further facilitates future application of high-energy-density Li metal batteries.

MiR-382 inhibits cell growth and invasion by targeting NR2F2 in colorectal cancer.

Colorectal cancer (CRC) is one of the leading causes of cancer death worldwide. MiR-382 has been found to have a decreased expression and the ability to suppress tumorigenesis in certain cancers. However, the role of miR-382 in CRC has not been sufficiently investigated. NR2F2 (also known as COUP-TFII), a member of the steroid/thyroid receptor superfamily, is often aberrantly activated in various tumors, but it is currently unclear whether NR2F2 may be a target of miR-382. In the present study, we investigated the role of miR-382 in CRC and identified the regulation of NR2F2 by miR-382. We observed that miR-382 was aberrantly downregulated in CRC. Transfection with miR-382 mimics impeded the growth, migration, and invasion of CRC cells. The direct binding of miR-382 to the 3' untranslated region (3' UTR) of NR2F2 was confirmed using a luciferase reporter gene assay. We showed that the relative expression levels of NR2F2 were significantly higher in CRC tissues compared with normal adjacent mucosa. A Kaplan-Meier analysis indicated that patients with high NR2F2 expression had a poor overall survival. Knockdown of NR2F2 inhibited CRC cell growth, migration, and invasion. Ectopic expression of NR2F2 mitigated miR-382 suppression of CRC cell proliferation, migration, and invasion. In conclusion, the present study describes a potential mechanism underlying a miR-382/NR2F2 link contributing to CRC development. Our results demonstrate that miR-382 represents a potential strategy against CRC. © 2016 Wiley Periodicals, Inc.

The long noncoding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1.

Colon cancer-associated transcript-1 (CCAT1) is a highly conserved long noncoding RNA that is deregulated in several cancers. However, its role in gastric carcinoma and its post-transcriptional regulation remain poorly understood. In this study, we provide the first evidence that CCAT1 regulates miR-490 in gastric cancer (GC) cells. Interestingly, miR-490 can also repress CCAT1 expression. CCAT1 expression was significantly upregulated, and miR-490 expression was downregulated in GC. The negative correlation between miR-490 and CCAT1 expression was observed in GC tissues. Importantly, CCAT1 contains a putative miR-490-binding site, and deletion of this binding site abolishes their miR-490 responsiveness. Post-transcriptional CCAT1 silencing by miR-490 significantly suppressed GC cell migration. Furthermore, miR-490 directly bound to the hnRNPA1 mRNA 3'-UTR to repress its translation. Inhibition of miR-490 rescued CCAT1 siRNA-mediated suppression of cell migration. hnRNPA1 expression was significantly upregulated in GC specimens, and there was a negative correlation between miR-490 and hnRNPA1 expression and also a positive correlation between hnRNAP1 expression level and CCAT1 level. Taken together, we show for the first time that the CCAT1/miR-490/hnRNPA1 axis promotes GC migration, and it may have a possible diagnostic and therapeutic potential in GC.

Preparation of chitosan resin by two-step crosslinking method and its adsorption for palladium in wastewater.

To preserve the activity of amine groups on chitosan, chitosan resin (CR) was synthesized using the reversed-phase suspension two-step crosslinking method for the adsorption of palladium from wastewater. The effects of varying the amounts of chitosan, liquid paraffin, ethyl acetate, formaldehyde solution, and epichlorohydrin on the adsorption capacity of CR were investigated using both single-factor experiments and response surface methodology. The preparation conditions for the chitosan resin were optimized, and its adsorption properties were systematically evaluated. The results indicated that CR exhibited a high saturated adsorption capacity for palladium, reaching 195.22 mg·g-1. The adsorption kinetics followed the pseudo-second-order model, while the adsorption isotherms were well described by the Sips model. Thermodynamic analysis demonstrated that the adsorption process was spontaneous and endothermic. Furthermore, CR maintained exceptional stability, with a palladium removal efficiency exceeding 99.8 % even after eight adsorption-desorption cycles. The primary adsorption mechanism is attributed to the interaction between palladium ions and the protonated amino groups of the chitosan resin.

A superstable, flexible, and scalable nanofluidic ion regulation composite membrane.

Two-dimensional layered membranes with high and stable ion transport properties have various applications in nanofluidic devices; however, their construction remains a considerable challenge. Herein, we develop a superstable aramid nanofiber/graphite composite membrane with numerous one-dimensional and two-dimensional nano-confined interspaces for ultrafast ion transport. The fabricated flexible and scalable membrane exhibits high tensile strength (∼115.3 MPa) even after immersion in water for 90 days. Further, the aramid nanofiber/graphite conductor features the surface-charge-governed ion transport behavior. The ionic conductivity of the membrane at a low potassium chloride concentration of 10-4 mol/L can be enhanced by 16 times that of the bulk counterpart. More importantly, its structure and ionic conductivity remain unchanged even after immersion in different harsh solutions (e.g., acid, base, and ethanol) for over 30 days. Molecular dynamics simulations reveal that the superstability of the membrane is attributable to the robust interchain interactions within the aramid nanofibers and the strong interfacial interactions between the aramid nanofibers and graphite nanosheets. This study highlights the superior structural stability of the proposed flexible and scalable aramid nanofiber/graphite composite membrane, which could be employed in advanced nanofluidic devices for application under extreme working environments.

Self-assembled hyaluronic acid-coated nanocomplexes for targeted delivery of curcumin alleviate acute kidney injury.

Acute kidney injury (AKI) is a pathological process with high morbidity, and drug resistance is easy to occur due to untargeted drug therapy. Curcumin can repair acute kidney injury. The expression of the CD44 receptor in renal tubular epithelial cells is abnormally elevated during AKI, and hyaluronic acid (HA) has the ability to bind specifically to the CD44 receptor. In this study, we developed a hyaluronic acid-coated liposome (HALP) nanocomplexes that targeted renal epithelial cells and its effect of relieving AKI was investigated. HALP was formed by self-assembly through the electrostatic interaction of curcumin-loaded cationic liposomes (LP) with hyaluronic acid and responds to the release of curcumin in the acidic microenvironment of lesions to treat AKI. HALP had good stability and biocompatibility. The in vitro results showed that compared to LP, HALP exhibited higher antioxidant, anti-inflammatory, and anti-apoptotic capacities. The AKI model suggested that HALP could not only target and accumulate in the injured kidney but also had an excellent ability to reduce the inflammatory response, which decreased tubular necrosis and restored kidney function.

Production of gas-releasing electrolyte-replenishing Ah-scale zinc metal pouch cells with aqueous gel electrolyte.

Aqueous zinc batteries are ideal candidates for grid-scale energy storage because of their safety and low-cost aspects. However, the production of large-format aqueous Zn batteries is hindered by electrolyte consumption, hydrogen gas evolution and accumulation, and Zn dendrites growth. To circumvent these issues, here we propose an "open" pouch cell design for large-format production of aqueous Zn batteries, which can release hydrogen gas and allow the refilling of the electrolyte components consumed during cell cycling. The cell uses a gel electrolyte containing crosslinked kappa (k)-carrageenan and chitosan. It bonds water molecules and hinders their side reaction with Zn, preventing electrolyte leakage and fast evaporation. As a proof-of-concept, we report the assembly and testing of a Zn | |ZnxV2O5·nH2O multi-layer "open" pouch cell using the carrageenan/chitosan gel electrolyte, which delivers an initial discharge capacity of 0.9 Ah and 84% capacity retention after 200 cycles at 200 mA g‒1, 370 kPa and 25 °C.

[Tachyarrhythmia caused by low-dose norepinephrine: a case report].

Norepinephrine (NE) can raise blood pressure and speed up heart rate. However, because its effect of raising heart rate is less than that of reflex reduction of heart rate caused by the increase of blood pressure, NE causes more heart rate decrease in patients. A case of tachyarrhythmia caused by low dose NE was admitted to department of intensive care unit (ICU) of Shijiazhuang Third Hospital. The heart rate of the patient increased with the elevation of NE application dose. A variety of antiarrhythmic drugs was invalid. The related examination was prescribed to eliminate the cause of arrhythmia caused by the disorder of electrolysis and thyroid function, and found that heart rate decreased as the dose of NE tapered. After NE was stopped, the patient recovered sinus rhythm. During one month of follow-up, the patient's heart rhythm was normal. Therefore, the occurrence of tachyarrhythmia is related to NE.

Removal of tar derived from biomass gasification via synergy of non-thermal plasma and catalysis.

In this study, the reforming of toluene, as a surrogate for tar, was investigated in plasma-alone (PA) and plasma-catalytic (PC) systems. The effects of feed gas oxygen content (O2/(O2 + N2) = 0, 3, 12, 21, or 30 vol%) and the discharge power (30, 75, or 90 W) on toluene conversion, the selectivity of syngas (H2 + CO), and undesirable liquid by-products were evaluated using the PA system. A maximum toluene conversion of 87.9% and a minimum selectivity of undesirable liquid by-products of 0.53% for ethylbenzene, and 1.24% for benzene, were obtained when the discharge power was 90 W and the oxygen content in the carrier gas was 3 vol%. However, a maximum gas selectivity of 48.4% for H2 and 19.4% for CO was attained when the discharge power was 75 W and the oxygen content was 3 vol% and 12 vol%, respectively. The effect of the steam/carbon molar ratio (S/C) on toluene reforming was investigated using the PC system with Ni/ZSM-5 catalyst under a discharge power of 75 W. The addition of steam to the feed gas significantly enhanced the conversion of toluene to syngas. A maximum toluene conversion of 88.5% was reached with a minimum selectivity of liquid by-products (1.9% for ethylbenzene and 5.2% for benzene) when S/C was 2. However, the highest selectivity of syngas (69.8% for H2 and 21.2% for CO) was achieved when S/C was 2.5. The catalyst employed in the plasma reforming of toluene exhibited excellent anti-carbon deposition performance. A possible reaction mechanism and pathway of toluene destruction was proposed based on analysis of both gaseous and liquid products.

Single Atom Array Mimic on Ultrathin MOF Nanosheets Boosts the Safety and Life of Lithium-Sulfur Batteries.

The development of Li-S batteries is largely impeded by the growth of Li dendrites and polysulfide shuttling. To solve these two problems simultaneously, herein the study reports a "single atom array mimic" on ultrathin metal organic framework (MOF) nanosheet-based bifunctional separator for achieving the highly safe and long life Li-S batteries. In the designed separator, the periodically arranged cobalt atoms coordinated with oxygen atoms (CoO4 moieties) exposed on the surface of ultrathin MOF nanosheets, "single atom array mimic", can greatly homogenize Li ion flux through the strong Li ion adsorption with O atoms at the interface between anode and separator, leading to stable Li striping/plating. Meantime, at the cathode side, the Co single atom array mimic serves as "traps" to suppress polysulfide shuttling by Lewis acid-base interaction. As a result, the Li-S coin cells with the bifunctional separator exhibit a long cycle life with an ultralow capacity decay of 0.07% per cycle over 600 cycles. Even with a high sulfur loading of 7.8 mg cm-2 , an areal capacity of 5.0 mAh cm-2 can be remained after 200 cycles. Moreover, the assembled Li-S pouch cell displays stable cycling performance under various bending angles, demonstrating the potential for practical applications.

Highly Elastic Hydrated Cellulosic Materials with Durable Compressibility and Tunable Conductivity.

Anisotropic cellular materials with direction-dependent structure and durable mechanical properties enable various applications (e.g., nanofluidics, biomedical devices, tissue engineering, and water purification), but their widespread use is often hindered by complex and scale-limited fabrication and unsatisfactory mechanical performance. Here, inspired by the anisotropic and hierarchical material structure of tendons, we demonstrate a facile, scalable top-down approach for fabricating a highly elastic, ionically conductive, anisotropic cellulosic material (named elastic wood) directly from natural wood via chemical treatment. The resulting elastic wood demonstrates good elasticity and durable compressibility, showing no sign of fatigue after 10 000 compression cycles. The chemical treatment not only softens the wood cell walls by partially removing lignin and hemicellulose but introduces an interconnected cellulose fibril network into the wood channels. Atomistic and continuum modeling further reveals that the absorbed water can freely and reversibly move inside the elastic wood and therefore helps the elastic wood accommodate large compressive deformation and recover to its original shape upon compression release. In addition, the elastic wood showed a high ionic conductivity of up to 0.5 mS cm-1 at a low KCl concentration of 10-4 M, which can be tuned by changing the compression ratio of the material. The demonstrated elastic, mechanically robust, and ionically conductive cellulosic material combining inherited anisotropic cellular structure from natural wood and a self-formed internal gel may find a variety of potential applications in ionic nanofluidics, sensors, soft robots, artificial muscle, environmental remediation, and energy storage.

Plasma-enhanced steam reforming of different model tar compounds over Ni-based fusion catalysts.

Tar formation during biomass gasification is undesirable due to the decreased energy efficiency and increased costs for maintaining downstream equipment. The hybrid non-thermal plasma-catalysis method is considered to be a promising alternative, since it overcomes the disadvantages arising from both catalyst deactivation during catalytic reforming and the formation of undesirable liquid by-products in plasma reforming. SiO2- and ZSM-5-supported Ni-based catalysts with different Ni loadings (0.5, 1, 3, and 5 wt%) were prepared by thermal fusion and applied to the steam reforming of toluene. Different characterizations of fresh and spent catalysts including XRD, H2-TPR, N2 adsorption-desorption, SEM, TEM, XPS and TGA were conducted to show the properties of catalysts. The results indicated that Ni/ZSM-5 exhibited better performance than Ni/SiO2, due to the increased dispersion of Ni particles and the stronger metal-support interaction of Ni/ZSM-5, which was confirmed by the TEM and H2-TPR results. In addition, the performances of the catalysis-only (CatO), plasma-only (PlO), and in-plasma-catalysis (IPC) systems in steam reforming of different model tar compounds including benzene, toluene, furfural, naphthalene, fluorene and pyrene were compared using Ni(5 wt%)/ZSM-5. Obvious synergistic effects between DBD plasma and Ni(5 wt%)/ZSM-5 was observed for syngas production in the IPC system.

Fly-through synthesis of nanoparticles on textile and paper substrates.

The fast and efficient synthesis of nanoparticles on flexible and lightweight substrates is increasingly critical for various medical and wearable applications. However, conventional high temperature (high-T) processes for nanoparticle synthesis are intrinsically incompatible with temperature-sensitive substrates, including textiles and paper (i.e. low-T substrates). In this work, we report a non-contact, 'fly-through' method to synthesize nanoparticles on low-T substrates by rapid radiative heating under short timescales. As a demonstration, textile substrates loaded with platinum (Pt) salt precursor are rapidly heated and quenched as they move across a 2000 K heating source at a continuous production speed of 0.5 cm s-1. The rapid radiative heating method induces the thermal decomposition of various precursor salts and nanoparticle formation, while the short duration ensures negligible change to the respective low-T substrate along with greatly improved production efficiency. The reported method can be generally applied to the synthesis of metal nanoparticles (e.g. gold and ruthenium) on various low-T substrates (e.g. paper). The non-contact and continuous 'fly-through' synthesis offers a robust and efficient way to synthesize supported nanoparticles on flexible and lightweight substrates. It is also promising for ultrafast and roll-to-roll manufacturing to enable viable applications.

A radiative cooling structural material.

Reducing human reliance on energy-inefficient cooling methods such as air conditioning would have a large impact on the global energy landscape. By a process of complete delignification and densification of wood, we developed a structural material with a mechanical strength of 404.3 megapascals, more than eight times that of natural wood. The cellulose nanofibers in our engineered material backscatter solar radiation and emit strongly in mid-infrared wavelengths, resulting in continuous subambient cooling during both day and night. We model the potential impact of our cooling wood and find energy savings between 20 and 60%, which is most pronounced in hot and dry climates.

MicroRNA-103 suppresses glioma cell proliferation and invasion by targeting the brain-derived neurotrophic factor.

Glioma is the most common and aggressive of malignant brain tumours. MicroRNAs (miRNAs/miRs) are involved in tumour development of various human cancers, including glioma. Therefore, miRNAs may have potential tumour diagnostic, prognostic and therapeutic values in human glioma. miR­103 is abnormally expressed in various human cancer types. However, the detailed expression pattern, biological functions and underlying molecular mechanism of miR­103 in glioma remain unclear. Therefore, the present study aimed to investigate the expression, biological roles and underlying mechanisms of miR­103 in glioma. Results of the present study demonstrated that miR­103 was significantly down­regulated in glioma tissues and cell lines. Functional experiments demonstrated that miR­103 overexpression inhibited the proliferation and invasion of glioma cells in vitro. Additionally, brain­derived neurotrophic factor (BDNF) was identified as a direct functional target of miR­103 in glioma. Furthermore, mRNA and protein expression levels of BDNF were highly upregulated in glioma tissues compared with normal brain tissues. Spearman's correlation analysis indicated a negative association between miR­103 and BDNF mRNA expression levels in glioma tissues. Furthermore, rescue experiments demonstrated that BDNF up­regulation reversed the suppressive effects of miR­103 on glioma cell proliferation and invasion. Therefore, the authors of the present study hypothesized that the interaction between miR­103 and BDNF serves a role in glioma progression and, in the future, may serve as a therapeutic target for glioma treatment.

Isotropic Paper Directly from Anisotropic Wood: Top-Down Green Transparent Substrate Toward Biodegradable Electronics.

Flexible electronics have found useful applications in both the scientific and industrial communities. However, substrates traditionally used for flexible electronics, such as plastic, cause many environmental issues. Therefore, a transparent substrate made from natural materials provides a promising alternative because it can be degraded in nature. The traditional bottom-up fabrication method for transparent paper is expensive, environmentally unfriendly, and time-consuming. In this work, for the first time, we developed a top-down method to fabricate isotropic, transparent paper directly from anisotropic wood. The top-down method includes two steps: a delignification process to bleach the wood by lignin removal and a pressing process for removing light-reflecting and -scattering sources. The resulting isotropic, transparent paper has high transmittance of about 90% and high haze over 80% and is demonstrated as a nature-disposable substrate for electronic/optical devices. Adjusting the pressing ratio used changes the density of the resulting paper, which tunes the microstructure-related properties of the isotropic, transparent paper. This top-down method is simple, fast, environmentally friendly, and cost-effective, which can greatly promote the development of paper-based green optical and electronic devices.

From Wood to Textiles: Top-Down Assembly of Aligned Cellulose Nanofibers.

Advanced textiles made of macroscopic fibers are usually prepared from synthetic fibers, which have changed lives over the past century. The shortage of petrochemical resources, however, greatly limits the development of the textile industry. Here, a facile top-down approach for fabricating macroscopic wood fibers for textile applications (wood-textile fibers) comprising aligned cellulose nanofibers directly from natural wood via delignification and subsequent twisting is demonstrated. Inherently aligned cellulose nanofibers are well retained, while the microchannels in the delignified wood are squeezed and totally removed by twisting, resulting in a dense structure with approximately two times higher mechanical strength (106.5 vs 54.9 MPa) and ≈20 times higher toughness (7.70 vs 0.36 MJ m-3 ) than natural wood. Dramatically different from natural wood, which is brittle in nature, the resultant wood-textile fibers are highly flexible and bendable, likely due to the twisted structures. The wood-textile fibers also exhibit excellent knitting properties and dyeability, which are critical for textile applications. Furthermore, functional wood-textile fibers can be achieved by preinfiltrating functional materials in the delignified wood film before twisting. This top-down approach of fabricating aligned macrofibers is simple, scalable, and cost-effective, representing a promising direction for the development of smart textiles and wearable electronics.