[Serotonin binding by various populations of peritoneal cells and blood leukocytes from intact and thymectomized mice]. / Sviazyvanie serotonina raznymi populiatsiiami peritoneal'nykh kletok i leikotsitov krovi intaktnykh i timéktomirovannykh myshei.

Specific activity of 3H-serotonin binding with adhesive cells, as well as with T-lymphocyte enriched cells and B-lymphocytes, obtained from peritoneal excudate and blood leucocytes of CBA mice was determined. The highest affinity to amine was established in peritoneal T2 cells and adhesive blood cells. Results of studying the action of imipramin and blocators of some known serotonin receptors on amine binding with the investigated cells allow a conclusion on structural differences of serotonin-binding centres located on T-lymphocyte enriched cells and B-lymphocytes depending of their localization. The reduction of serotonin binding by immunocytes during ageing processes with animals (particularly tymectomized) was revealed, and this is supposed to be correlated with changes of immunological reactivity with ageing. It is supposed that serotonin participates in cooperative interactions of immunocytes in blood and peritoneal cavity.

[Study of the stability of morphogenetic substances in Acetabularia]. / Izuchenie stabil'nosti morfogeneticheskikh veshchestv u atsetabuliarii.

It was shown by means of interspecific transplantation that the morphogenetic substances in the A. crenulata cytoplasm kept their ability to determine the species specific morphogenesis in the A. mediterranea fragments during not less than 30 days. The isolation of the A. crenulata cytoplasm from the cell resulted in the complete inactivation of morphogenetic substances within 2--3 hrs. The removal of chloroplasts from the injected cytoplasm did not change its morphogenetic activity. The cytoplasm treatment by exogenous ribonuclease accelerated the inactivation of morphogenetic substances; this suggests its ribonucleic nature.

[RNA synthesis gradients in the nucleus-free fragments of Acetabularia mediterranea under conditions of local illumination]. / Gradienty sinteza RNK u bez'iadernykh fragmentov Acetabularia meditarranea v usloviiakh lokal'nogo osveshcheniia.

The intensity of RNA synthesis was studied in different regions of anuclear fragments of the Acetabularia mediterranea stem under their local illumination. The local illumination was shown to activate RNA synthesis and formation of distal-medial gradients of this synthesis in the illuminated regions of the fragments to a much greater extent than in their darkened regions. The formation of the gradient in the illuminated region took place even if the growth in this region was insignificant and was not accompanied by the cap formation. The results obtained suggests the absence of obligatory correspondence between the ability of morphogenesis and the gradient of RNA synthesis.

[The isolation and expression in E. coli of a recombinant plasmid containing the 3'-terminal fragment of the cDNA of the influenza A virus nucleoprotein gene]. / Poluchenie i ékspressiia v E. coli rekombinantnoi plazmidy, soderzhashchei 3'-kontsevoi fragment kDNK gena nukleoproteina virusa grippa A.

A recombinant plasmid pN62 determining the synthesis of a hybrid protein consisting of a full-size beta-galactosidase and C-terminus fragment of influenza A virus nucleoprotein was constructed. The complete identity of pN62 insert with the 3'-terminus cDNA fragment of influenza A virus NP-gene and conservation of beta-galactosidase reading frame was confirmed by Maxam-Gilbert sequencing of pN62. An expression of pN62 plasmid in E. coli JM103 in the presence of IPTG resulted in accumulation of fused protein as poorly soluble inclusion bodies in the bacterial cells. Analysis of E. coli JM103/pN62 bacteria lysates by 7% PAGE revealed that molecular weight of hybrid polypeptide was 18 kDa heavier than normal beta-galactosidase and corresponded to the previously deduced weight of 135 kDa.

[Comparative study of the phagocytosis of sheep erythrocytes by the macrophages of inbred mice differing in their sensitivity to immunogenic stimulation by the given antigen]. / Sravnitel'noe izuchenie fagotsitoza éritrotsitov barana makrofagami lineinykh myshei, kontrastnykh po chuvstvitel'nosti k immunogennoi stimuliatsii dannym antigenom.

The phagocytosis of 14C-labeled sheep red blood cells (SRBC) by the macrophages of isogeneic CBA, BALB/c and C57BL/6 mice was studied. In the presence of bovine serum the macrophages of CBA mice were found to ingest SRBC significantly less actively than the macrophages of BALB/c and C57BL/6 mice. In the presence of isologous serum the macrophages of mice belonging to the strains under study showed quite comparable characteristics with respect to their capacity of ingesting SRBC. The duration of the intracellular digestion of SRBC by the macrophages of mice of these strains did not vary in different strains irrespective of the type of serum.

[Specific binding of serotonin by blood leukocytes and peritoneal cells in the mouse]. / Spetsificheskoe sviazyvanie serotonina leikotsitami krovi i peritoneal'nymi kletkami myshei.

It was shown that blood leukocytes and peritoneal cells of mice specifically bind serotonin (5-hydroxytryptamine) in amounts which exceed manyfold its binding by the cells of thymus, lymphatic nodes, spleen, Peyer's patches and bone marrow. The nature of this binding suggests that peritoneal cells and blood leukocytes contain cell populations (apart from those already known ones) capable of binding and trapping of serotonin.

[Peculiarities of specific binding of serotonin by blood leukocytes, peritoneal cells and synaptosomes of mice]. / Osobennosti spetsificheskogo sviazyvaniia serotonina leikotsitami krovi, peritoneal'nymi kletkami i sinaptosomami myshei.

The properties of serotonin-active sites were studied on peritoneal cells, blood leukocytes and synaptosomes of mice (CBA line). Treatment of cell suspensions with EDTA, ouabain, strophanthin, 2,4-dinitrophenol, dithiothreitol and trypsin demonstrated that serotonin binding by peritoneal cells and leukocytes depends on bivalent ions and K+, Na+-pump operation, requires energy and intact disulfide bonds and is determined by a protein structure. ATP and ADP were found to inhibit amine adsorption by peritoneal cells. These cells specifically bind ATP 10 times more intensively than leukocytes. The data obtained are suggestive of differences in the composition of serotonin-active structures of blood leukocytes, peritoneal cells and synaptosomes.

[Characteristics of serotonin binding by the cells of immunocompetent tissues and synaptosomes]. / Nekotorye kharakteristiki sviazyvaniia serotonina kletkami immunokompetentnykh tkanei i sinaptosomami.

The ratio of surface and intracellular serotonin binding by the cells of immunocompetent tissues and synaptosomes of immunized and intact CBA mice was studied by treatment with imipramine inhibiting serotonin penetration through the cytoplasmic membrane. As early as 5 minutes after the antigen injection the content of intracellular amine increased with changes in its binding by the cytoplasmic membrane. The probability of the functional interconnection between the reaction of immunocompetent tissues to the antigen and two forms of serotonin binding by the cells of these tissues is discussed.

[Use of liposomes for incorporation of alkylating derivatives of mono- and oligonucleotides into mammalian cells]. / Ispol'zpvanie liposom dlia vvedeniia alkiliruiushchikh proizvodnykh monoi oligonukleotidov v kletki mlekopitaiushchikh.

It was shown that within the liposomes mono- and oligonucleotides and their alkylating derivatives penetrate the cells of Ehrlich ascite carcinoma and peritoneal exudate of the mice. Inside the cells the alkylating reagents are mainly utilized for modification of proteins (42--76%), RNA (5--16%) and DNA (3--9%). Presumably DNA modification is largely dependent on the penetration of the reagents into the nuclei. No significant differences in alkylation of the cell components by oligoadenylate derivatives, capable of complementary interactions with nucleic acids and mononucleotide derivatives, incapable of such interactions, were observed.