Influence of metal speciation on soil ecotoxicity impacts in life cycle assessment.

It is unknown whether metallic elements remain important contributors to terrestrial ecotoxicity impact scores in life cycle assessment (LCA) when solid- and liquid-phase speciation are considered in environmental fate, exposure and effects. Here, a new speciation-based method for calculating comparative toxicity potentials (CTP) of 23 metallic elements in soils was compared with two other widely used methods which do not consider speciation (i.e., IMPACT, 2002+ and ReCiPe 2008) for nearly 13,000 life cycles of unit processes taken from different sectors. Differences in impact scores between method were driven either by differences in characterization models (ReCiPe 2008) or both by differences in characterization models and substance coverage (IMPACT, 2002+). Strong correlations (r > 0.98) and seemingly constant shifts in impact scores were found for those processes where one or few substances (usually metals) contributed most to total impact and there were large differences in CTPs between methods for these substances. Weaker correlations but often better agreement in impact scores were found for those processes where organic substances were dominant contributors to total impact. Our results suggest that metals are expected to remain important contributors to soil ecotoxicity impacts in LCA when speciation is considered.

Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation.

Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80%. (i.e. [P66614][Br] and [P66614][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.

Limitations of experiments performed in artificially made OECD standard soils for predicting cadmium, lead and zinc toxicity towards organisms living in natural soils.

Development of comparative toxicity potentials of cationic metals in soils for applications in hazard ranking and toxic impact assessment is currently jeopardized by the availability of experimental effect data. To compensate for this deficiency, data retrieved from experiments carried out in standardized artificial soils, like OECD soils, could potentially be tapped as a source of effect data. It is, however, unknown whether such data are applicable to natural soils where the variability in pore water concentrations of dissolved base cations is large, and where mass transfer limitations of metal uptake can occur. Here, free ion activity models (FIAM) and empirical regression models (ERM, with pH as a predictor) were derived from total metal EC50 values (concentration with effects in 50% of individuals) using speciation for experiments performed in artificial OECD soils measuring ecotoxicological endpoints for terrestrial earthworms, potworms, and springtails. The models were validated by predicting total metal based EC50 values using backward speciation employing an independent set of natural soils with missing information about ionic composition of pore water, as retrieved from a literature review. ERMs performed better than FIAMs. Pearson's r for log10-transformed total metal based EC50s values (ERM) ranged from 0.25 to 0.74, suggesting a general correlation between predicted and measured values. Yet, root-mean-square-error (RMSE) ranged from 0.16 to 0.87 and was either smaller or comparable with the variability of measured EC50 values, suggesting modest performance. This modest performance was mainly due to the omission of pore water concentrations of base cations during model development and their validation, as verified by comparisons with predictions of published terrestrial biotic ligand models. Thus, the usefulness of data from artificial OECD soils for global-scale assessment of terrestrial ecotoxic impacts of Cd, Pb and Zn in soils is limited due to relatively small variability of pore water concentrations of dissolved base cations in OECD soils, preventing their inclusion in development of predictive models. Our findings stress the importance of considering differences in ionic composition of soil pore water when characterizing terrestrial ecotoxicity of cationic metals in natural soils.

The influence of bioaugmentation and biosurfactant addition on bioremediation efficiency of diesel-oil contaminated soil: feasibility during field studies.

The study focused on assessing the influence of bioaugmentation and addition of rhamnolipids on diesel oil biodegradation efficiency during field studies. Initial laboratory studies (measurement of emitted CO2 and dehydrogenase activity) were carried out in order to select the consortium for bioaugmentation as well as to evaluate the most appropriate concentration of rhamnolipids. The selected consortium consisted of following bacterial taxa: Aeromonas hydrophila, Alcaligenes xylosoxidans, Gordonia sp., Pseudomonas fluorescens, Pseudomonas putida, Rhodococcus equi, Stenotrophomonas maltophilia, Xanthomonas sp. It was established that the application of rhamnolipids at 150 mg/kg of soil was most appropriate in terms of dehydrogenase activity. Based on the obtained results, four treatment methods were designed and tested during 365 days of field studies: I) natural attenuation; II) addition of rhamnolipids; III) bioaugmentation; IV) bioaugmentation and addition of rhamnolipids. It was observed that bioaugmentation contributed to the highest diesel oil biodegradation efficiency, whereas the addition of rhamnolipids did not notably influence the treatment process.

Tribological Performance of Composites Reinforced with the Agricultural, Industrial and Post-Consumer Wastes: A Review.

Waste management is still one of the leading global challenges in the 21st century. From the European Union's point of view, the Waste Framework Directive obliges businesses and households to recycle at least 55% of their municipal waste by 2025 and to reach 65% in 2035. Hence there is a great need to seek new solutions for the reuse of various waste materials. One of the most widely used wastes is their utilization as fillers or reinforcements in the metal- or polymer-based composites. The reuse of wastes for the production of tribological materials gives not only environmental benefits related to the transformation of waste into raw materials but also may improve the mechanical and tribological properties of such materials. Moreover, the use of waste reduces the production costs resulting from the lower price of filler materials and longer service life of developed products. The purpose of the current review is, therefore, aimed at the evaluation of the reuse of agricultural, industrial and postconsumer wastes as reinforcements in the composites used for tribological applications. The tribological performance (wear rate, coefficient of friction) of both monolithic and hybrid composites reinforced with waste materials was a particular subject of interest in this review.

Evaluating robustness of a diesel-degrading bacterial consortium isolated from contaminated soil.

It is not known whether diesel-degrading bacterial communities are structurally and functionally robust when exposed to different hydrocarbon types. Here, we exposed a diesel-degrading consortium to model either alkanes, cycloalkanes or aromatic hydrocarbons as carbon sources to study its structural resistance. The structural resistance was low, with changes in relative abundances of up to four orders of magnitude, depending on hydrocarbon type and bacterial taxon. This low resistance is explained by the presence of hydrocarbon-degrading specialists in the consortium and differences in growth kinetics on individual hydrocarbons. However, despite this low resistance, structural and functional resilience were high, as verified by re-exposing the hydrocarbon-perturbed consortium to diesel fuel. The high resilience is either due to the short exposure time, insufficient for permanent changes in consortium structure and function, or the ability of some consortium members to be maintained during exposure on degradation intermediates produced by other members. Thus, the consortium is expected to cope with short-term exposures to narrow carbon feeds, while maintaining its structural and functional integrity, which remains an advantage over biodegradation approaches using single species cultures.