[Expression of leucocyte cell-surface antigens on colon cancer cell line HR8348].

OBJECTIVE: To define the immune phenotype of colon cancer cells. METHODS: Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining. RESULTS: HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR. CONCLUSION: The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.