RESUMO
Five years after the US anthrax attacks, and more than two years after BioShield legislation was ratified, a survey reveals that biodefense funding has thus far produced only a handful of products for clinical development.
Assuntos
Biotecnologia/economia , Bioterrorismo/economia , Defesa Civil/economia , Medicina Clínica/economia , Indústria Farmacêutica/economia , Transferência de Tecnologia , Vacinas/economia , Bioterrorismo/prevenção & controle , Estados UnidosAssuntos
Biotecnologia/economia , Biotecnologia/tendências , Empreendedorismo/economia , Empreendedorismo/tendências , Investimentos em Saúde/economia , Coleta de Dados , Empreendedorismo/organização & administração , Empreendedorismo/estatística & dados numéricos , Investimentos em Saúde/tendências , Propriedade/economia , Propriedade/tendências , Estados UnidosAssuntos
Biotecnologia/economia , Biotecnologia/tendências , Financiamento de Capital/economia , Financiamento de Capital/tendências , Empreendedorismo/economia , Empreendedorismo/tendências , Investimentos em Saúde/economia , Investimentos em Saúde/tendências , Biotecnologia/estatística & dados numéricos , Financiamento de Capital/métodos , Financiamento de Capital/estatística & dados numéricos , Empreendedorismo/estatística & dados numéricos , Europa (Continente) , Investimentos em Saúde/estatística & dados numéricos , Propriedade/economia , Propriedade/estatística & dados numéricos , Propriedade/tendênciasRESUMO
Phosphorus (P) acquisition and partitioning are essential for plant homeostasis. P is available for plant uptake when in its inorganic form (H2PO4-, or Pi), but Pi is often limiting in soils. Plants secrete acid phosphatases (APases) into the apoplastic space, which may be important for obtaining Pi from organic P sources; however, the relative importance of these enzymes for plant P nutrition has yet to be determined. We demonstrate that the root-associated APase pool is increased in Arabidopsis when Pi is limiting and document five APase isoforms secreted from Arabidopsis roots. Previously, we presented the identification of the phosphatase under-producer (pup) mutants, which have decreased in vivo root APase staining when grown under low P conditions. Here, we present the characterization of one of these, pup3, and further studies with pup1. pup3 has 49%, 38%, and 37% less specific APase activity in exudates, roots, and shoots, respectively. Root-associated APase activity is decreased by 16% in pup1 and 25% in pup3, regardless of P treatment. Two APase activity isoforms are reduced in pup3 exudates, and root and shoot isoforms are also affected. One of the two exudate isoforms is recognized by a polyclonal antibody raised to an Arabidopsis purple APase recombinant protein (AtPAP12); however, AtPAP12 transcript levels are unaffected in the mutant. The pup3 mutation was mapped to 68.4 +/- 6.0 centimorgans on chromosome 5. Although P concentrations were not altered in pup1 and pup3 tissues when grown in nutrient solution in which Pi was the sole source of P, the mutants had 10% (pup1) and 17% (pup3) lower shoot P concentrations when grown in a peat-vermiculite mix in which the majority of the total P was present as organic P. Therefore, the pup defects, which include secreted APases, are functionally important for plant P nutrition.