RESUMO
Variable requirements for actin during clathrin-mediated endocytosis (CME) may be related to regional or cellular differences in membrane tension. To compensate, local regulation of force generation may be needed to facilitate membrane curving and vesicle budding. Force generation is assumed to occur primarily through actin polymerization. Here we examine the role of myosin II using loss of function experiments. Our results indicate that myosin II acts on cortical actin scaffolds primarily in the plane of the plasma membrane (bottom arrow) to generate changes that are critical for enhancing CME progression.
Assuntos
Clatrina/fisiologia , Endocitose/fisiologia , Miosina Tipo II/fisiologia , Actinas/metabolismo , Animais , Camundongos , Camundongos Knockout , Músculos/fisiologia , Miosina Tipo II/genética , Transferrina/metabolismoRESUMO
During mammalian development, neuromuscular junctions and some other postsynaptic cells transition from multiple- to single-innervation as synaptic sites are exchanged between different axons. It is unclear whether one axon invades synaptic sites to drive off other inputs or alternatively axons expand their territory in response to sites vacated by other axons. Here we show that soon-to-be-eliminated axons rapidly reverse fate and grow to occupy vacant sites at a neuromuscular junction after laser removal of a stronger input. This reversal supports the idea that axons take over sites that were previously vacated. Indeed, during normal development we observed withdrawal followed by takeover. The stimulus for axon growth is not postsynaptic cell inactivity because axons grow into unoccupied sites even when target cells are functionally innervated. These results demonstrate competition at the synaptic level and enable us to provide a conceptual framework for understanding this form of synaptic plasticity.
Assuntos
Junção Neuromuscular/fisiologia , Sinapses/fisiologia , Animais , Axônios/fisiologia , Camundongos , Camundongos Transgênicos , Neurônios Motores/fisiologia , Plasticidade Neuronal/fisiologiaRESUMO
Recent evidence suggests that endocytosis, not exocytosis, can be rate limiting for neurotransmitter release at excitatory CNS synapses during sustained activity and therefore may be a principal determinant of synaptic fatigue. At low stimulation frequencies, the probability of synaptic release is linked to the probability of synaptic retrieval such that evoked release results in proportional retrieval even for release of single synaptic vesicles. The exact mechanism by which the retrieval rates are coupled to release rates, known as compensatory endocytosis, remains unknown. Here we show that inactivation of presynaptic myosin II (MII) decreases the probability of synaptic retrieval. To be able to differentiate between the presynaptic and postsynaptic functions of MII, we developed a live cell substrate patterning technique to create defined neural circuits composed of small numbers of embryonic mouse hippocampal neurons and physically isolated from the surrounding culture. Acute application of blebbistatin to inactivate MII in circuits strongly inhibited evoked release but not spontaneous release. In circuits incorporating both control and MIIB knock-out cells, loss of presynaptic MIIB function correlated with a large decrease in the amplitude of evoked release. Using activity-dependent markers FM1-43 and horseradish peroxidase, we found that MII inactivation greatly slowed vesicular replenishment of the recycling pool but did not impede synaptic release. These results indicate that MII-driven tension or actin dynamics regulate the major pathway for synaptic vesicle retrieval. Changes in retrieval rates determine the size of the recycling pool. The resulting effect on release rates, in turn, brings about changes in synaptic strength.
Assuntos
Endocitose/fisiologia , Miosina Tipo II/metabolismo , Neurotransmissores/metabolismo , Sinapses/metabolismo , Transmissão Sináptica/fisiologia , Animais , Hipocampo/metabolismo , Camundongos , Camundongos Knockout , Microscopia Eletrônica de Transmissão , Neurônios/metabolismo , Técnicas de Patch-Clamp , Vesículas Sinápticas/metabolismoRESUMO
Histology is the study of the structure of biological tissue using microscopy techniques. As digital imaging technology advances, high resolution microscopy of large tissue volumes is becoming feasible; however, new interactive tools are needed to explore and analyze the enormous datasets. In this paper we present a visualization framework that specifically targets interactive examination of arbitrarily large image stacks. Our framework is built upon two core techniques: display-aware processing and GPU-accelerated texture compression. With display-aware processing, only the currently visible image tiles are fetched and aligned on-the-fly, reducing memory bandwidth and minimizing the need for time-consuming global pre-processing. Our novel texture compression scheme for GPUs is tailored for quick browsing of image stacks. We evaluate the usability of our viewer for two histology applications: digital pathology and visualization of neural structure at nanoscale-resolution in serial electron micrographs.
Assuntos
Gráficos por Computador , Técnicas Histológicas/estatística & dados numéricos , Processamento de Imagem Assistida por Computador/estatística & dados numéricos , Humanos , Microscopia Eletrônica de Transmissão/estatística & dados numéricosRESUMO
Guidance cues and signal transduction mechanisms acting at the nerve growth cone are fairly well understood, but the intracellular mechanisms operating to change the direction of axon outgrowth remain unknown. We now show that growth cones integrate myosin II-dependent contraction for rapid, coordinated turning at borders of laminin stripes in response to signals from laminin-activated integrin receptors; in the absence of myosin II activity, outgrowth continues across the borders.
Assuntos
Diferenciação Celular/fisiologia , Movimento Celular/fisiologia , Cones de Crescimento/metabolismo , Laminina/metabolismo , Miosina Tipo II/metabolismo , Sistema Nervoso/embriologia , Citoesqueleto de Actina/metabolismo , Animais , Adesão Celular/fisiologia , Comunicação Celular/fisiologia , Células Cultivadas , Sinais (Psicologia) , Cones de Crescimento/ultraestrutura , Integrinas/metabolismo , Internet , Camundongos , Camundongos Knockout , Fatores de Crescimento Neural/metabolismo , Sistema Nervoso/citologia , Sistema Nervoso/metabolismo , Neuritos/metabolismo , Transdução de Sinais/fisiologia , Gravação em VídeoRESUMO
Affiliation 4 incorrectly read 'University of the Basque Country (Ikerbasque), University of the Basque Country and Donostia International Physics Center, San Sebastian 20018, Spain.'Also, the affiliations of Ignacio Arganda-Carreras with 'IKERBASQUE, Basque Foundation for Science, Bilbao, 48013, Spain' and 'Donostia International Physics Center (DIPC), San Sebastian, 20018, Spain' were inadvertently omitted.Additionally, the third sentence of the first paragraph of the Results section entitled 'Multicontrast organ-scale imaging with ChroMS microscopy' incorrectly read 'For example, one can choose lambda1 = 850 and lambda2 = 110 nm for optimal two-photon excitation of blue and red chromophores.'. The correct version reads 'lambda2 = 1100 nm' instead of 'lambda2 = 110 nm'. These errors have now been corrected in the PDF and HTML versions of the Article.
RESUMO
Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
Assuntos
Córtex Cerebral/diagnóstico por imagem , Imageamento Tridimensional/métodos , Proteínas Luminescentes/química , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Neuroimagem/métodos , Animais , Astrócitos/metabolismo , Córtex Cerebral/citologia , Cor , Dependovirus , Feminino , Vetores Genéticos/administração & dosagem , Vetores Genéticos/genética , Células HEK293 , Humanos , Proteínas Luminescentes/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Modelos Animais , Nestina/genética , Técnicas de Rastreamento Neuroanatômico/métodos , Parvovirinae/genética , Células Piramidais/metabolismo , TransfecçãoRESUMO
Nerve growth factor (NGF) promotes growth, differentiation, and survival of sensory neurons in the mammalian nervous system. Little is known about how NGF elicits faster axon outgrowth or how growth cones integrate and transform signal input to motor output. Using cultured mouse dorsal root ganglion neurons, we found that myosin II (MII) is required for NGF to stimulate faster axon outgrowth. From experiments inducing loss or gain of function of MII, specific MII isoforms, and vinculin-dependent adhesion-cytoskeletal coupling, we determined that NGF causes decreased vinculin-dependent actomyosin restraint of microtubule advance. Inhibition of MII blocked NGF stimulation, indicating the central role of restraint in directed outgrowth. The restraint consists of myosin IIB- and IIA-dependent processes: retrograde actin network flow and transverse actin bundling, respectively. The processes differentially contribute on laminin-1 and fibronectin due to selective actin tethering to adhesions. On laminin-1, NGF induced greater vinculin-dependent adhesion-cytoskeletal coupling, which slowed retrograde actin network flow (i.e., it regulated the molecular clutch). On fibronectin, NGF caused inactivation of myosin IIA, which negatively regulated actin bundling. On both substrates, the result was the same: NGF-induced weakening of MII-dependent restraint led to dynamic microtubules entering the actin-rich periphery more frequently, giving rise to faster elongation.
Assuntos
Actomiosina/metabolismo , Cones de Crescimento/fisiologia , Microtúbulos/metabolismo , Fator de Crescimento Neural/fisiologia , Animais , Crescimento Celular , Feminino , Fibronectinas/fisiologia , Gânglios Espinais/citologia , Laminina/fisiologia , Masculino , Camundongos Knockout , Miosina não Muscular Tipo IIA/metabolismo , Miosina não Muscular Tipo IIB/metabolismo , Fosforilação , Processamento de Proteína Pós-Traducional , Técnicas de Cultura de Tecidos , Proteínas rho de Ligação ao GTP/metabolismo , Proteína rhoA de Ligação ao GTPRESUMO
Although fluorescently labeled structures can be analyzed more easily at high resolution in fixed-tissue preparations than in living animals, some biological questions can only be answered by time-lapse imaging. Changes in nervous system wiring during development cannot be determined reliably by taking tissue from different animals at staggered time points. Rather, the same cells and connections must be viewed repeatedly. To study developmental synapse elimination, we image muscles in transgenic mice that express fluorescent proteins in motor neurons and follow the same neuromuscular junctions (NMJs) over multiple days. This protocol describes the use of confocal microscopy for in vivo imaging of developing NMJs in transgenic neonatal mice expressing cyan fluorescent protein (CFP) or yellow fluorescent protein (YFP). The sternomastoid, a flat, accessible neck muscle with large junctions, is imaged. A principal advantage of confocal microscopy is the ability to acquire multiple fluorescence channels simultaneously. If the channels are acquired sequentially, there is inevitably misalignment because of movement. Moreover, the total imaging time scales linearly with the number of channels. With simultaneous acquisition, only a single scan may be required. With perfect alignment between channels, irrespective of movement that might occur during a scan, color differences can be used to study interactions between axons over time. A limitation of this technique is that axons must be brightly labeled and at the muscle surface. NMJs that are more than one muscle fiber deep may be difficult to scan because of index of refraction changes that cause image blurring.
Assuntos
Processamento de Imagem Assistida por Computador/métodos , Microscopia Confocal/métodos , Junção Neuromuscular/embriologia , Imagem com Lapso de Tempo/métodos , Animais , Expressão Gênica , Proteínas Luminescentes/biossíntese , Camundongos , Camundongos Transgênicos , Coloração e Rotulagem/métodosRESUMO
In vivo imaging is the most direct way to uncover the dynamic events that occur during neural development. This approach is especially challenging in developing mammals where technical hurdles related to optical resolution, animal movement, phototoxicity, and postoperative complications need to be addressed. In our work concerning the process of naturally occurring synapse elimination at developing neuromuscular junctions, these technical issues are critical because we need to resolve multiple and very fine single axons that converge on the same synaptic site. In previous studies, we used wide-field microscopy with either intensified or high quantum efficiency cameras. We now have begun to use laser scanning confocal microscopy which improves contrast and resolution but comes with its own challenges. In this chapter, we describe the approaches we have taken to permit both rapid time-lapse (minutes to hours) and long-term time-lapse (days to months) to visualize the synaptic alterations associated with the development and maturation of the neuromuscular system.