Oxidative stress, DNA damage, and mutagenicity induced by the extractable organic matter of airborne particulates on bacterial models.

The biological activity induced by the extractable organic matter (EOM) of size-segregated airborne Particulate Matter (PM) from two urban sites, urban traffic (UT) and urban background (UB), was assessed by using bacterial assays. The Gram-negative Escherichia coli (E. coli) coliform bacterium was used to measure the intracellular formation of Reactive Oxygen Species (ROS) by employing the Nitroblue tetrazolium (NBT) reduction assay and the lipid peroxidation by malondialdehyde (MDA) measurement. To the best of our knowledge, this is the first study using E. coli for assessing the bioactivity of ambient air in term of oxidative mechanism studies. E. coli BL21 cells were further used for DNA damage assessment by employing the reporter (ß-galactosidase) gene expression assay. The bacterial strain S. typhimurium TA100 was used to assess the mutagenic potential of PM by employing the well-known mutation assay (Ames test). Four PM size fractions were assessed for bioactivity, specifically the quasi-ultrafine mode (<0.49 µm), the upper accumulation mode (0.49-0.97 µm), the upper fine mode (0.97-3 µm), and the coarse mode (>3.0 µm). The EOM of each PM sample included three organic fractions of successively increased polarity: the non-polar organic fraction (NPOF), the moderately polar organic fraction (MPOF), and the polar organic fraction (POF). The toxicological endpoints induced by each organic fraction were correlated with the concentrations of various organic chemical components determined in previous studies in an attempt to identify the chemical classes involved.

In vitro cellular toxicity induced by extractable organic fractions of particles exhausted from urban combustion sources - Role of PAHs.

The bioactivity of the extractable organic matter (EOM) of particulate matter (PM) exhausted from major urban combustion sources, including residential heating installations (wood-burning fireplace and oil-fired boiler) and vehicular exhaust from gasoline and diesel cars), was investigated in vitro by employing multiple complementary cellular and bacterial assays. Cytotoxic responses were investigated by applying the MTT ((3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide)) bioassay and the lactate dehydrogenase (LDH) release bioassay on human lung cells (MRC-5). Sister Chromatids Exchange (SCE) genotoxicity was measured on human peripheral lymphocytes. Lipid peroxidation potential via reactive oxygen species (ROS) was evaluated on E. coli bacterial cells by measuring the malondialdehyde (MDA) end product. Furthermore, the DNA damage induced by the organic PM fractions was evaluated by the reporter (ß-galactosidase) gene expression assay in the bacterial cells, and, by examining the fragmentation of chromosomal DNA on agarose gel electrophoresis. The correlations between the source PM-induced biological endpoints and the PM content in polycyclic aromatic hydrocarbons (PAHs), as typical molecular markers of combustion, were investigated. Fireplace wood smoke particles exhibited by far the highest content in total and carcinogenic PAHs followed by oil boilers, diesel and gasoline emissions. However, in all bioassays, the total EOM-induced toxicity, normalized to PM mass, was highest for diesel cars equipped with Diesel Particle Filter (DPF). No correlation between the toxicological endpoints and the PAHs content was observed suggesting that cytotoxicity and genotoxicity are probably driven by other extractable organic compounds than the commonly measured unsubstituted PAHs. Clearly, further research is needed to elucidate the role of PAHs in the biological effects induced by both, combustion emissions, and ambient air particles.

Redox activity and in vitro bioactivity of the water-soluble fraction of urban particulate matter in relation to particle size and chemical composition.

Chemical and toxicological characterization of the water-soluble fraction of size-segregated urban particulate matter (PM) (<0.49, 0.49-0.97, 0.97-1.5, 1.5-3.0, 3.0-7.2 and >7.2 µm) was carried out at two urban sites, traffic and urban background, during the cold and the warm period. Chemical analysis of the water-soluble PM fraction included ionic species (NO3(-), SO4(2-), Cl(-), Na(+), NH4(+), K(+), Mg(2+), Ca(2+)), water-soluble organic carbon (WSOC), and trace elements (Al, As, Ba, Cd, Cr, Cu, Fe, Pb, Mn, Ni, Zn, Pt, Pd, Rh, Ru, Ir, Ca, and Mg). The dithiothreitol (DTT) assay was employed for the abiotic assessment of the oxidative PM activity. Cytotoxic responses were investigated in vitro by applying the mitochondrial dehydrogenase (MTT) and the lactate dehydrogenase (LDH) bioassays on human lung cells (MRC-5), while DNA damage was estimated by the single cell gel electrophoresis assay, known as Comet assay. The correlations between the observed bioactivity responses and the concentrations of water-soluble chemical PM constituents in the various size ranges were investigated. The results of the current study corroborate that short-term bioassays using lung human cells and abiotic assays, such as the DTT assay, could be relevant to complete the routine chemical analysis and to obtain a preliminary screening of the potential effects of PM-associated airborne pollutants on human health.

Cytotoxicity and genotoxicity induced in vitro by solvent-extractable organic matter of size-segregated urban particulate matter.

Three organic fractions of different polarity, including a non polar organic fraction (NPOF), a moderately polar organic fraction (MPOF), and a polar organic fraction (POF) were obtained from size-segregated (<0.49, 0.49-0.97, 0.97-3 and >3 µm) urban particulate matter (PM) samples, and tested for cytotoxicity and genotoxicity using a battery of in vitro assays. The cytotoxicity induced by the organic PM fractions was measured by the mitochondrial dehydrogenase (MTT) cell viability assay applied on MRC-5 human lung epithelial cells. DNA damages were evaluated through the comet assay, determination of the poly(ADP-Ribose) polymerase (PARP) activity, and the oxidative DNA adduct 8-hydroxy-deoxyguanosine (8-OHdG) formation, while pro-inflammatory effects were assessed by determination of the tumor necrosis factor-alpha (TNF-α) mediator release. In addition, the Sister Chromatid Exchange (SCE) inducibility of the solvent-extractable organic matter was measured on human peripheral lymphocyte. Variations of responses were assessed in relation to the polarity (hence the expected composition) of the organic PM fractions, particle size, locality, and season. Organic PM fractions were found to induce rather comparable Cytotoxicity and genotoxicity of PM appeared to be rather independent from the polarity of the extractable organic PM matter (EOM) with POF often being relatively more toxic than NPOF or MPOF. All assays indicated stronger mass-normalized bioactivity for fine than coarse particles peaking in the 0.97-3 and/or the 0.49-0.97 µm size ranges. Nevertheless, the air volume-normalized bioactivity in all assays was highest for the <0.49 µm size range highlighting the important human health risk posed by the inhalation of these quasi-ultrafine particles.