Postoperative Analgesic Effects of Laserpuncture and Meloxicam in Bitches Submitted to Ovariohysterectomy.

BACKGROUND: This prospective, randomised and blind study investigated the efficacy of laserpuncture for postoperative pain management in dogs. METHOD: Sixteen bitches were sedated with acepromazine and randomly treated before ovariohysterectomy with meloxicam 0.2 mg·kg-1 intramuscular or laserpuncture (wavelength 904 mm, frequency 124 Hz, potency 10 Joules, 100 s in each acupoint). Anaesthesia was performed with propofol, isoflurane/O2, and fentanyl. The Glasgow Composite Measure Pain Scale (GCMPS) and Dynamic Interactive Visual Analog Scale (DIVAS) were used to evaluate postoperative pain before and for 24 h after surgery. Morphine was administrated as rescue analgesia when pain scores were ≥3.33 (GCMPS). Differences between treatments, time points, and amount of rescue analgesia between groups were investigated by the Mann-Whitney test and the area under the curve (AUC) for GCMPS, Friedman, and Chi-squared tests, respectively (p < 0.05). RESULTS: Dogs treated with laserpuncture presented lower GCMPS AUC for 24 h and lower GCMPS scores at 2 and 4 h postoperatively (p = 0.04). Three dogs treated with meloxicam required postoperatively rescue analgesia against none treated with laserpuncture. CONCLUSIONS: In this preliminary study, laserpuncture mitigated postoperative pain in dogs following ovariohysterectomy, and the technique is a promising adjunct to perioperative pain management in dogs undergoing soft tissue surgery.

Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period.

Bitches with uteri devoid of endometrial glands should be sterile, and consequently could contribute to the population control of dogs. Considering that an inadequate exposure of the female reproductive system to steroids can lead to the formation of the uterine gland knock-out (UGKO) phenotype in some species, the aim of this study was to evaluate the effect of serial applications of medroxyprogesterone acetate (MPA) from birth until the age of six months on the development of endometrial glands in bitches. For this purpose, 16 female mongrel dogs from different litters were distributed into either an MPA group (n = 8), animals treated with 10 mg kg sc (Promone-E®, Pfizer, Brasil) at 3-week intervals, from day one after birth until the age of six months, or a control group (n = 8), composed of animals that only received a 0.9% NaCl solution in place of MPA. At six months of age, ovariohysterectomy was performed and uterine horn samples were collected for histological and immunohistochemical examinations. The bitches from the MPA-treated group presented a 35% decrease in the number of endometrial glands, a larger diameter of the endometrial glands, a greater epithelial height, as well as a greater thickness of the uterine wall, endometrium, and myometrium. However, no significant differences were observed between the two groups in the expression of ER-α, ER-ß, and PR on the surface epithelium and endometrial stroma. Therefore, the serial application of MPA from birth until the age of 6 months do not completely ablate the development of the endometrial glands in bitches, but impair it by 35%.

Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats.

Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher's exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 ± 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 ± 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate.

Coprodutos da agroindústria na alimentação de leitões / Agro-industrial co-products in feed for piglets

Foi realizado um experimento para determinar a composição química e digestibilidade dos nutrientes e energia do resíduo de batata palha (RBP), resíduo de pipoca doce (RPD), resíduo de biscoito de trigo doce (RBT), e resíduo de biscoito de trigo salgado (RBS) para leitões. Foram determinados os valores de matéria seca (MS), proteína bruta (PB), extrato etéreo (EE), matéria mineral (MM), cálcio (Ca), fósforo (P), sódio (Na) e energia bruta (EB). A digestibilidade dos alimentos foi avaliada utilizando 18 suínos alojados em gaiolas de metabolismo durante dois períodos consecutivos de 12 dias, sendo sete para adaptação e cinco destinados à coleta total de fezes. Os resultados de PB obtidos foram relativamente baixos, sendo 7,2; 6,0; 6,8 e 3,4% para o RBP, RPD, RBT e RBS, respectivamente. Os conteúdos de EE foram de 39,64; 7,12; 12,40; e 22,28% para o RBP, RPD, RBT e RBS, respectivamente. Os valores de Ca e P foram baixos e, em todos os coprodutos, inferiores a 0,18%. Teores elevados de Na foram encontrados no RBP e RBS (0,9 e 1,36%, respectivamente). A EB foi de 6.065; 4.199; 4.494; e 5.032 kcal kg-1 para RBP, RPD, RBT e RBS, respectivamente. Os valores de ED foram de 5.686; 3.995; 3.916 e 4.953 kcal kg-1, para RBP, RPD, RBT, e RBS e mostraram correlação linear e positiva (r2=0,97; P<0,05) com o teor de EE dos coprodutos. Estudos adicionais são necessários para estabelecer os teores ótimos de inclusão dos coprodutos analisados nas dietas de suínos. Contudo, os resultados de composição química e digestibilidade dos nutrientes e energia permitem concluir que os resíduos de batata palha, pipoca doce, biscoito de trigo doce e biscoito de trigo salgado possuem potencial para serem utilizados na alimentação de leitões.

One experiment was realized to determine the chemical composition and digestibility of nutrients and energy of shoestring potatoes residues (SP), sweet popcorn residues (SPC), wheat sweet biscuit residues (WSB) and wheat salted sticks residues (WSS) for piglets. The dry matter (DM), crude protein (CP), ether extract (EE), mineral matter (MM), calcium (Ca), phosphorus (P) and sodium (Na) and gross energy (GE) were measured. Eighteen castrated pigs penned in metabolic crates during two consecutive periods of 12 days (seven to adaptation and five to feces collection) were used to evaluate the digestibility´s coefficients of feedstuffs. The SP, SPC, WSB, and WSS had relatively low CP values 7.2; 6.0; 6.8 and 3.4%, respectively. The EE contents were 39.64; 7.12; 12.40 and 22.28% for SP, SPC; WSB and WSS, respectively. All the co-products evaluated had low values of Ca and P and anyone had a Ca and P value higher than 0.18% and the SPC and WSS had high Na levels (0.9 and 1.36%, respectively). The GE were 5.686; 3.995; 3.916; 4.953kcal kg-1 for SP, SPC; WSB and WSS, respectively and showed positive linear correlation (r2=0.97; P<0.05) with EE levels. Additional studies are necessary to establish the optimal levels of co-products to include in pigs diets. However, the results of chemical composition and digestibility of nutrients and energy indicate that shoestring potatoes residues, sweet popcorn residues, wheat sweet biscuit residues and wheat salted sticks residues have potential to be used in piglet's diets.