Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
Cell ; 165(7): 1776-1788, 2016 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-27238022

RESUMO

A major challenge in understanding the cellular diversity of the brain has been linking activity during behavior with standard cellular typology. For example, it has not been possible to determine whether principal neurons in prefrontal cortex active during distinct experiences represent separable cell types, and it is not known whether these differentially active cells exert distinct causal influences on behavior. Here, we develop quantitative hydrogel-based technologies to connect activity in cells reporting on behavioral experience with measures for both brain-wide wiring and molecular phenotype. We find that positive and negative-valence experiences in prefrontal cortex are represented by cell populations that differ in their causal impact on behavior, long-range wiring, and gene expression profiles, with the major discriminant being expression of the adaptation-linked gene NPAS4. These findings illuminate cellular logic of prefrontal cortex information processing and natural adaptive behavior and may point the way to cell-type-specific understanding and treatment of disease-associated states.


Assuntos
Comportamento Animal , Mapeamento Encefálico/métodos , Córtex Pré-Frontal/citologia , Animais , Comportamento Apetitivo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Cocaína/administração & dosagem , Eletrochoque , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Córtex Pré-Frontal/metabolismo
2.
Sci Rep ; 7(1): 5899, 2017 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-28724969

RESUMO

Three-dimensional tissue-structural relationships are not well captured by typical thin-section histology, posing challenges for the study of tissue physiology and pathology. Moreover, while recent progress has been made with intact methods for clearing, labeling, and imaging whole organs such as the mature brain, these approaches are generally unsuitable for soft, irregular, and heterogeneous tissues that account for the vast majority of clinical samples and biopsies. Here we develop a biphasic hydrogel methodology, which along with automated analysis, provides for high-throughput quantitative volumetric interrogation of spatially-irregular and friable tissue structures. We validate and apply this approach in the examination of a variety of developing and diseased tissues, with specific focus on the dynamics of normal and pathological pancreatic innervation and development, including in clinical samples. Quantitative advantages of the intact-tissue approach were demonstrated compared to conventional thin-section histology, pointing to broad applications in both research and clinical settings.


Assuntos
Doença , Imageamento Tridimensional/métodos , Organogênese , Animais , Feminino , Humanos , Hidrogéis/química , Camundongos Endogâmicos C57BL , Crista Neural/citologia , Sistemas Neurossecretores/citologia , Pâncreas/citologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA