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Ductal carcinoma in situ (DCIS) is a common precursor of invasive breast cancer. Our understanding of its genomic progression to recurrent disease remains poor, partly due to challenges associated with the genomic profiling of formalin-fixed paraffin-embedded (FFPE) materials. Here, we developed Arc-well, a high-throughput single-cell DNA-sequencing method that is compatible with FFPE materials. We validated our method by profiling 40,330 single cells from cell lines, a frozen tissue, and 27 FFPE samples from breast, lung, and prostate tumors stored for 3-31 years. Analysis of 10 patients with matched DCIS and cancers that recurred 2-16 years later show that many primary DCIS had already undergone whole-genome doubling and clonal diversification and that they shared genomic lineages with persistent subclones in the recurrences. Evolutionary analysis suggests that most DCIS cases in our cohort underwent an evolutionary bottleneck, and further identified chromosome aberrations in the persistent subclones that were associated with recurrence.
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Neoplasias da Mama , Carcinoma Ductal de Mama , Carcinoma Intraductal não Infiltrante , Feminino , Humanos , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/genética , Carcinoma Intraductal não Infiltrante/genética , Carcinoma Intraductal não Infiltrante/patologia , Progressão da Doença , Genômica/métodos , Análise da Expressão Gênica de Célula Única , Linhagem Celular TumoralRESUMO
Microdroplet single-cell ATAC-seq is widely used to measure chromatin accessibility, however, highly scalable and simple sample multiplexing procedures are not available. Here, we present a transposome-assisted single nucleus barcoding approach for ATAC-seq (SNuBar-ATAC) that utilizes a single oligonucleotide adaptor for multiplexing samples during the existing tagmentation step and does not require a pre-labeling procedure. The accuracy and scalability of SNuBar-ATAC was evaluated using cell line mixture experiments. We applied SNuBar-ATAC to investigate treatment-induced chromatin accessibility dynamics by multiplexing 28 mice with lung tumors that received different combinations of chemo, radiation, and targeted immunotherapy. We also applied SNuBar-ATAC to study spatial epigenetic heterogeneity by multiplexing 32 regions from a human breast tissue. Additionally, we show that SNuBar can multiplex single cell ATAC and RNA multiomic assays in cell lines and human breast tissue samples. Our data show that SNuBar is a highly accurate, easy-to-use, and scalable system for multiplexing scATAC-seq and scATAC and RNA co-assay experiments.
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Montagem e Desmontagem da Cromatina , Cromatina/metabolismo , Epigênese Genética , Regulação Neoplásica da Expressão Gênica , Heterogeneidade Genética , Neoplasias Pulmonares/metabolismo , Análise de Célula Única , Fatores de Transcrição/metabolismo , Animais , Antineoplásicos/farmacologia , Quimiorradioterapia , Cromatina/genética , Sequenciamento de Cromatina por Imunoprecipitação , Feminino , Humanos , Células K562 , Cinética , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/terapia , Masculino , Camundongos da Linhagem 129 , RNA-Seq , Dosagem Radioterapêutica , Fatores de Transcrição/genéticaRESUMO
Our knowledge of copy number evolution during the expansion of primary breast tumours is limited1,2. Here, to investigate this process, we developed a single-cell, single-molecule DNA-sequencing method and performed copy number analysis of 16,178 single cells from 8 human triple-negative breast cancers and 4 cell lines. The results show that breast tumours and cell lines comprise a large milieu of subclones (7-22) that are organized into a few (3-5) major superclones. Evolutionary analysis suggests that after clonal TP53 mutations, multiple loss-of-heterozygosity events and genome doubling, there was a period of transient genomic instability followed by ongoing copy number evolution during the primary tumour expansion. By subcloning single daughter cells in culture, we show that tumour cells rediversify their genomes and do not retain isogenic properties. These data show that triple-negative breast cancers continue to evolve chromosome aberrations and maintain a reservoir of subclonal diversity during primary tumour growth.
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Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Proliferação de Células , Células Clonais/metabolismo , Células Clonais/patologia , Evolução Molecular , Sequência de Bases , Linhagem Celular Tumoral , Linhagem da Célula , Aberrações Cromossômicas , Variações do Número de Cópias de DNA/genética , Análise Mutacional de DNA , Instabilidade Genômica/genética , Humanos , Perda de Heterozigosidade/genética , Modelos Genéticos , Taxa de Mutação , Imagem Individual de Molécula , Análise de Célula Única , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/patologiaRESUMO
A theoretical channel impulse response (CIR) model of short-range non-line-of-sight (NLOS) ultraviolet communications (UVC) in noncoplanar geometry under the single-scatter condition is proposed. Simulation results obtained from the widely accepted Monte-Carlo (MC)-based channel model of NLOS UVC are provided to verify corresponding theoretical results obtained from the proposed theoretical single-scatter CIR model. Additionally, an outdoor experiment with a light-emitting diode (LED) as the light source is first designed to measure the channel step response of NLOS UVC and to further validate the proposed theoretical single-scatter CIR model. By varying the different parameters of the transmitter and the receiver, such as the baseline range, the inclination angle, the azimuth angle, the beam divergence angle, and the field-of-view angle, the results of the proposed theoretical single-scatter CIR model and the MC-based channel model are exhibited and further analyzed in detail. Results indicate that the computational time cost by the proposed theoretical single-scatter CIR model is decreased to less than 0.6% of the MC-based one with comparable accuracy in assessing the temporal characteristics of NLOS UVC channels. Additionally, theoretical results obtained from the proposed theoretical single-scatter CIR model manifest satisfactory agreement with corresponding experimental measurements.
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Objective: The assessment of liver cancer lesion characteristics mainly relies on multi-row spiral computed tomography (MDCT) and magnetic resonance imaging (MRI). MDCT suffers from a series of problems, such as low soft tissue contrast and large tumor boundary errors, which lead to its limited practical application value in liver cancer. In contrast, Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced MRI has better soft-tissue contrast than MDCT and increases the clarity of liver cancer lesions. To investigate the differences between MDCT and Gd-EOB-DTPA-enhanced MRI in managing patients with hepatocellular liver cancer. Methods: A total of 80 patients diagnosed with hepatocellular carcinoma of the liver, who received treatment at our hospital between September 2020 and September 2022, were included in this study. These patients were evenly divided into two groups: the observation group and the control group, with 40 cases in each. The aim of this study was to compare the differences in signal indices of hepatobiliary stage between the two groups in patients with hepatocellular carcinoma. Results: A total of 89 cancer nodules were detected in patients by MDCT, and 109 cancer nodules were detected in patients by Gd-EOB-DTPA-enhanced MRI. When the number of nodules detected by both imaging modalities was statistically analyzed, the differences in the number of hepatocellular carcinoma (HCC) nodules detected by MDCT and Gd-EOB-DTPA-enhanced MRI were statistically significant (P < .05). Further analysis of the data by single cancer nodule, multiple cancer nodules, and cancer nodule size showed that the difference between the two imaging modalities was statistically significant (P < .05) in the diagnosis of patients with a single liver cancer nodule or multiple liver cancer nodules (94.8% vs.81.3%). The difference in the comparison was statistically significant (P < .05). Conclusion: Gd-EOB-DTPA-enhanced MRI demonstrates superior diagnostic efficacy in detecting small hepatocellular carcinoma, offers improved staging capabilities for hepatocellular carcinoma, and provides more precise guidance for treatment planning.Consequently, Gd-EOB-DTPA-enhanced MRI exhibits exceptional diagnostic value and serves as a valuable tool for guiding treatment decisions in patients with hepatocellular carcinoma.
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Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Meios de Contraste , Gadolínio DTPA , Imageamento por Ressonância Magnética/métodos , Estudos RetrospectivosRESUMO
Objective: Knee injuries are very common and may lead to other secondary injuries if effective treatment is lacking. In addition to standardized physical examination, magnetic resonance imaging (MRI) is sometimes considered an aid in the diagnosis of knee trauma. In order to have a more accurate diagnosis of knee injuries, we compared MRI with arthroscopic findings in this study to evaluate the diagnostic accuracy of MRI for meniscal tears and anterior cruciate ligament injuries of the knee. Methods: One hundred and ten patients with suspected meniscal tears and anterior cruciate ligament injuries of the knee who were admitted to our hospital from June 2020 to June 2022 were selected as study subjects, and the clinical data of the patients were retrospectively analyzed. All patients underwent MRI for preoperative diagnosis, and the sensitivity, specificity, MRI findings, and confirmation of diagnosis were compared and analyzed, and the accuracy of MRI in diagnosing meniscal tears and ACL injuries of the knee was analyzed. Results: The mean ACL angle was (98.0 ± 5.4) in the MRI group and (118.0 ± 6.8) in the arthroscopic group, the difference between the two groups was statistically significant P < .05. The mean L/H value of the ACL was (2.12 ± 0.38) in the MRI group and (1.81 ± 0.19) in the arthroscopic group, which was statistically different between the two groups (P < .05). Among the patients, 68 meniscal injuries were found in the MRI examination, including 45 cases of knee meniscal tears and 23 cases of anterior cruciate ligament injuries. The sensitivity, specificity, positive and negative predictive values, agreement rate, kappa value, and Youden index of MRI in diagnosing meniscal tears and ACL injuries were all high. Conclusions: In terms of sensitivity and accuracy, MRI is an excellent imaging technique for the diagnosis of meniscal tears and anterior cruciate ligament injuries of the knee.
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Lesões do Ligamento Cruzado Anterior , Traumatismos do Joelho , Menisco , Lesões do Menisco Tibial , Humanos , Lesões do Ligamento Cruzado Anterior/diagnóstico por imagem , Lesões do Ligamento Cruzado Anterior/complicações , Estudos Retrospectivos , Sensibilidade e Especificidade , Artroscopia/métodos , Lesões do Menisco Tibial/diagnóstico por imagem , Lesões do Menisco Tibial/complicações , Traumatismos do Joelho/diagnóstico por imagem , Traumatismos do Joelho/complicações , Imageamento por Ressonância Magnética/métodosRESUMO
Self-sweeping fiber lasers have carved out numerous applications such as spectral detection, fiber sensor, etc. In this work, we propose a single-frequency self-sweeping fiber laser with a few-longitudinal-mode range by employing a length of space path to achieve the function of intracavity ranging. Different from the previous design, a fiber collimator and mirror are utilized to act as the reflector, and the distance between them can be adjusted flexibly. Based on this design, we achieve a few-longitudinal-mode self-sweeping operation containing seven longitudinal modes. When the distance is set as a fixed value, the behaviors of fiber laser containing central wavelength, quasi-continuous wave pulse, as well as radio frequency spectrum at different pump power are measured. The intracavity ranging systems are also demonstrated at different distances between collimator and mirror, showing a promising accuracy. This work provides a new laser ranging tool and opens up the applied scenario of self-sweeping fiber laser.
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We study the pulse characteristics in a laser mode-locked by active modulators with non-Hermitian driven signals. The signal assembles a parity-time (P T) symmetric and an anti-parity-time (A P T) symmetric function with fundamental and harmonic frequencies, respectively, inducing the complex coupling between modes in the frequency domain. A one-dimensional synthetic lattice is used to analyze the spectral mode coupling. By enlarging the weight and harmonic order of the A P T part of the signal, the optical spectrum can be adjusted from redshift to blueshift. Simultaneously, the pulse duration and spectral width are shortened and broadened, respectively. The work explores the role of non-Hermitian modulation in the mode-locked laser area.
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We reported an all-polarization-maintaining single-frequency ytterbium-doped bidirectional fiber laser for the first time, to the best of our knowledge. Single-frequency operation was achieved by a stable dynamic grating in the active fiber of a proper length owing to the bidirectional operation of the laser. The fiber laser possesses a linewidth of 7.43 kHz, a slope efficiency of 47.9%, and a great long-term stability.
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We demonstrate for the first time, to our knowledge, an all-fiber erbium-doped mode-locked laser in which mode-locking (ML) is realized by the combination of nonlinear polarization rotation and a saturable dynamic filtering effect, thereby generating nearly transform-limited ultrashort pulses with a pulse duration and spectral width of 45.2 ps and 0.0775 nm, respectively. The laser achieves both ML and harmonic ML by increasing the pump power. Simultaneously, the filtering function is maintained by the saturable dynamic induced grating (SDIG) throughout the power-modulation process. Furthermore, numerical simulations are used to analyze the pulse energy evolution in the cavity, revealing the advantages of hybrid ML in decreasing the pulse duration and time-bandwidth product under narrow filtering status. This work proposes a practical method to achieve ultrafast laser pulses with a narrow bandwidth, solving the problem that the SDIG has a hard time realizing a stable ML sequence.
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In this article, we report, to the best of our knowledge, the first observation of the reverse self-sweeping phenomenon in an all-polarization-maintaining bidirectional ytterbium-doped fiber laser. Conventional behaviors, including the dependence of sweeping range, sweeping rate and average pulse repetition rate on the pump power, can be observed in our fiber laser. Two couplers with ratio of 50/50 and 10/90 are respectively employed as the output coupler in fiber laser, which generates the reverse self-sweeping phenomenon for comparison.
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We systematically study the stable-, period-N- and multiple-soliton regimes in an Erbium-doped fiber laser effectively mode-locked by nonlinear polarization rotation technique. In the stable mode-locked regime, an invariant soliton with 497 fs pulse duration and 6.9 nm optical spectrum are obtained. With a larger pump power of 180 mW, the period-N state (in which the pulse intensity returns to its original value after N cavity-roundtrips) emerges, accompanied by sub-sideband generation on the first Kelly sideband and spectrum shift. Considering the inconsistent central wavelengths between gain and polarization-dependent isolator (PD-ISO) firstly, to our knowledge, the numerical results are in good agreement with the experiment and reveal the composite filtering of gain and PD-ISO takes major responsibility for spectrum shift, which causes group velocity offset simultaneously. Further study shows the continued increase of pump power can lead to the laser operating in the unstable multi-pulse state and the narrow spectral width contributes to stabilizing the multi-pulse state. Our work can promote the understanding of soliton dynamics and filtering in ultrafast fiber lasers.
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Multiterritorial atherosclerosis has dramatically increased annual risk of adverse cardiovascular events than atherosclerotic disease with single-artery affected. Serum uric acid (SUA) is an important predictor of stroke and atherosclerosis; however, which is supported by few direct evidence based on cohort studies. A prospective cohort study including 2644 North Chinese adults aged ≥40 years was performed in 2010-2012 to investigate the association between SUA and multiterritorial vascular stenosis. Hyperuricaemia was defined as SUA levels >6 and >7 mg/dL for males and females, respectively. All participants underwent twice transcranial Doppler (TCD) and bilateral carotid duplex ultrasound to evaluate intracranial artery stenosis (ICAS) and extracranial arterial stenosis (ECAS) and peripheral arterial disease (PAD) was determined by ankle-brachial index (ABI) on January 2010 and January 2012 based on regular health check-ups. The cumulative incidence of vascular stenosis was significantly higher in subjects with hyperuricaemia than in those without hyperuricaemia (54.1% vs. 34.7%, P < 0.001). The adjusted odds ratios (ORs) with 95% confidence intervals (CIs) for new on-set vascular stenosis due to hyperuricaemia and a 1-mg/dL change in SUA level were 1.75 (1.32-2.31) and 1.29 (1.21-1.38), respectively. Furthermore, in the gender-stratified analysis, the association between SUA levels and ICAS was statistically significant in males (OR: 2.02; 95% CI: 1.18-3.46), but not females (OR: 0.85, 95% CI: 0.41-1.76, P for interaction: 0.026).
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Artérias/patologia , Aterosclerose/sangue , Ácido Úrico/sangue , Adulto , Povo Asiático , Aterosclerose/fisiopatologia , Estudos de Coortes , Constrição Patológica/sangue , Constrição Patológica/fisiopatologia , Feminino , Humanos , Hiperuricemia/sangue , Hiperuricemia/fisiopatologia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Doença Arterial Periférica/fisiopatologia , Estudos Prospectivos , Fatores de TempoRESUMO
BACKGROUND: NGS (next generation sequencing) has been widely used in studies of biological processes, ranging from microbial evolution to cancer genomics. However, the error rate of NGS (0.1 % ~ 1 %) is still remaining a great challenge for comprehensively investigating the low frequency variations, and the current solution methods have suffered severe amplification bias or low efficiency. RESULTS: We creatively developed Droplet-CirSeq for relatively efficient, low-bias and ultra-sensitive identification of variations by combining millions of picoliter uniform-sized droplets with Cir-seq. Droplet-CirSeq is entitled with an incredibly low error rate of 3 ~ 5 X 10(-6). To systematically evaluate the performances of amplification uniformity and capability of mutation identification for Droplet-CirSeq, we took the mixtures of two E. coli strains as specific instances to simulate the circumstances of mutations with different frequencies. Compared with Cir-seq, the coefficient of variance of read depth for Droplet-CirSeq was 10 times less (p = 2.6 X 10(-3)), and the identified allele frequency presented more concentrated to the authentic frequency of mixtures (p = 4.8 X 10(-3)), illustrating a significant improvement of amplification bias and accuracy in allele frequency determination. Additionally, Droplet-CirSeq detected 2.5 times genuine SNPs (p < 0.001), achieved a 2.8 times lower false positive rate (p < 0.05) and a 1.5 times lower false negative rate (p < 0.001), in the case of a 3 pg DNA input. Intriguingly, the false positive sites predominantly represented in two types of base substitutions (G- > A, C- > T). Our findings indicated that 30 pg DNA input accommodated in 5 ~ 10 million droplets resulted in maximal detection of authentic mutations compared to 3 pg (p = 1.2 X 10(-8)) and 300 pg input (p = 2.2 X 10(-3)). CONCLUSIONS: We developed a method namely Droplet-CirSeq to significantly improve the amplification bias, which presents obvious superiority over the currently prevalent methods in exploitation of ultra-low frequency mutations. Droplet-CirSeq would be promisingly used in the identification of low frequency mutations initiated from extremely low input DNA, such as DNA of uncultured microorganisms, captured DNA of target region, circulation DNA of plasma et al, and its creative conception of rolling circle amplification in droplets would also be used in other low input DNA amplification fields.
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Análise Mutacional de DNA/métodos , DNA Circular/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , DNA Bacteriano/genética , Escherichia coli , Frequência do Gene , Polimorfismo de Nucleotídeo ÚnicoRESUMO
Disinfection of hazardous microorganisms that may challenge environmental safety is a crucial issue for economic and public health. Here, we explore the potential of a novel electrochemical disinfectant named plasma activated water (PAW), which was generated by nonthermal plasma, for inactivating Staphylococcus aureus (S. aureus). Meanwhile, the influence of bovine serum albumin (BSA) on the PAW disinfection efficacy was investigated. In the presence of BSA, PAW treatments achieved a reduction of S. aureus ranging from 2.1 to 5.5 Log, when without BSA it reached 7 Log. The sterilization efficacy depended on the PAW treatment time of S. aureus and plasma activation time for PAW generation. The results of electron spin resonance spectra showed the concentrations of hydroxyl radical (OHâ¢) and nitric oxide radical (NOâ¢) in water activated by plasma for 10 min (10-PAW) were higher than those in water activated by plasma for 5 min (5-PAW). Additionally, the physiological analysis of S. aureus demonstrated that the integrity of cell membrane, membrane potential, and intracellular pH homeostasis as well as DNA structure were damaged by PAW, and the molecule structure and chemical bonds of S. aureus were also altered due to PAW. Thus, PAW can be a promising chemical-free and environmentally friendly electrochemical disinfectant for application in the medical and food industries.
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Desinfetantes/química , Desinfetantes/farmacologia , Desinfecção/métodos , Staphylococcus aureus/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Eletroquímica/métodos , Espectroscopia de Ressonância de Spin Eletrônica , Radical Hidroxila/química , Potenciais da Membrana/efeitos dos fármacos , Soroalbumina Bovina/química , Água/químicaRESUMO
Both irradiation and dislocations have been proposed as routes to rationally manipulate spatial distribution and micromorphology of precipitate. An interesting effect emerges in Fe-10at.%Cu-3at.%Mn-1.5at.%Ni-1.5at.%Al alloy due to the synergistic-competitive roles of dislocation loop and irradiation. Base on cascade mixing, vacancy-interstitial atoms and dislocation stress field model, we examine nucleation and growth dynamics of Cu-rich precipitates, where both dislocation loop and irradiation act in conjunction. Analytical treatments identify regimes, where the distribution of elements and point defects due to irradiation and dislocations are specific to the Cu-rich precipitates. Simulation results reveal that density, size and distribution of Cu-rich precipitates are a manifestation of the competing effects of the dislocation loop and the irradiation rate. More specifically, the dislocation loop preferentially assists the formation of precipitates and new dislocations at lower irradiation rates. Only the irradiation induces the formation of Cu-rich precipitates with the irradiation rate continues to increase. Equipped with molecular dynamics, where reproduces major interaction features of the solutes with point defects under displacement cascade, can verify multi-component morphologies of Cu-rich precipitates. This modeling framework provides an avenue to explore the role of dislocation loop and irradiation on the microstructural evolution of Cu-rich precipitates.
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Alzheimer's disease (AD) is the most common type of agerelated dementia, and causes progressive memory degradation, neuronal loss and brain atrophy. The pathological hallmarks of AD consist of amyloidß (Aß) plaque accumulation and abnormal neurofibrillary tangles. Amyloid fibrils are constructed from Aß peptides, which are recognized to assemble into toxic oligomers and exert cytotoxicity. The fibrillar Aßprotein fragment 2535 (Aß2535) induces local inflammation, thereby exacerbating neuronal apoptosis. Notoginsenoside R1 (NGR1), one of the primary bioactive ingredients isolated from Panax notoginseng, exhibits effective antiinflammatory and antioxidative activities. However, NGR1 pharmacotherapies targeting Aßinduced inflammation and cell injury cascade remain to be elucidated. The present study investigated the effect and mechanism of NGR1 in Aß2535treated PC12 cells. NGR1 doses between 250 and 1,000 µg/ml significantly increased cell viability suppressed by 20 µM Aß2535 peptide treatment. Notably, the present study demonstrated that Aß2535 peptideinduced sphingosine kinase 1 (SphK1) signaling activation was reduced after NGR1 treatment, further inhibiting the downstream NFκB inflammatory signaling pathway. In addition, administration of SphK1 inhibitor II (SKIII), a SphK1 inhibitor, also significantly reduced Aß2535 peptideinduced apoptosis and the ratio of NFκB pp65/p65. Furthermore, SphK1 knockdown in PC12 cells using small interfering RNA alleviated Aßinduced cell apoptosis and inflammation, suggesting a pivotal role of SphK1 signaling in the antiinflammatory effect of NGR1. In summary, NGR1 alleviated inflammation and apoptosis stimulated by Aß2535 by inhibiting the SphK1/NFκB signaling pathway and may be a promising agent for future AD treatment.
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Doença de Alzheimer , Ginsenosídeos , Animais , Ratos , Doença de Alzheimer/metabolismo , Anti-Inflamatórios/farmacologia , Apoptose , Ginsenosídeos/farmacologia , Ginsenosídeos/uso terapêutico , Inflamação/patologia , NF-kappa B/metabolismo , Células PC12 , Transdução de Sinais , Peptídeos beta-Amiloides/efeitos adversos , Peptídeos beta-Amiloides/farmacologiaRESUMO
Quinoa is a full-nutrition food; however, its poor flavor and small size make it not the best food option for direct consumption. In this study, a quinoa snack (QS, a cake) was developed, and the aroma profile of the products was improved by adding jujube fruit powder (made from dried jujube fruits, from 5% to 30%). Gas chromatography mass spectrum (GC-MS) combined with electronic nose (e-nose) was applied for characterizing the aroma profiles of QS samples. Results showed a total of 26 aroma compounds were identified in QS samples by GC-MS, and 3-methylbutanol (from 1525 µg/kg in QS-30 to 3487 µg/kg in QS-0), ethanol (from 1126 µg/kg in QS-0 to 3581 µg/kg in QS-30), hexanal (from 125.6 µg/kg in QS-30 to 984.1 µg/kg in QS-0), and acetaldehyde (from 531.9 µg/kg in QS-30 to 191.1 µg/kg in QS-0) were common. The e-nose response of W1S (sensitive to methane, from 17.50 of QS-0 to 93.85 of QS-30) and W1W (sensitive to sulfur-organic compounds of e-nose, from 15.57 of QS-0 to 39.50 of QS-30) were significantly higher, and significant differences were presented among QS samples. In conclusion, the aroma profile of the QS sample was significantly (p < .05) enhanced by the addition of jujube powder, and QS-30 with the highest jujube content (30%) presented the strongest aroma profile. Moreover, QS samples with different additions of jujube powders could be well distinguished by principal component analysis (PCA), and the combination of e-nose and GC-MS was effective in the volatile profile analysis of QS samples.
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Fruit tree leaves have different chemical compositions and diverse wax layer structures that result in different patterns of wetting and pesticide solution spreading on their surface. Fruit development is a time when pests and diseases occur, during which a large number of pesticides are needed. The wetting and diffusion properties of pesticide droplets on fruit tree leaves were relatively poor. To solve this problem, the wetting characteristics of leaf surfaces with different surfactants were studied. The contact angle, surface tension, adhesive tension, adhesion work, and solid-liquid interfacial tension of five surfactant solution droplets on jujube leaf surfaces during fruit growth were studied by the sessile drop method. C12E5 and Triton X-100 have the best wetting effects. Two surfactants were added to a 3% beta-cyfluthrin emulsion in water, and field efficacy tests were carried out on peach fruit moths in a jujube orchard at different dilutions. The control effect is as high as 90%. During the initial stage when the concentration is low, due to the surface roughness of the leaves, the surfactant molecules adsorbed at the gas-liquid and solid-liquid interfaces reach an equilibrium, and the contact angle on the leaf surface changes slightly. With increasing surfactant concentration, the pinning effect in the spatial structure on the leaf surface is overcome by liquid droplets, thereby significantly decreasing the contact angle. When the concentration is further increased, the surfactant molecules form a saturated adsorption layer on the leaf surface. Due to the existence of a precursor water film in the droplets, surfactant molecules on the interface continuously move to the water film on the surface of jujube tree leaves, thus causing interactions between the droplets and the leaves. The conclusion of this study provides theoretical guidance for the wettability and adhesion of pesticides on jujube leaves, so as to achieve the purpose of reducing pesticide use and improving pesticide efficacy.
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The clinical features and risk factors for survival time were analysed in haemodialysis patients complicated with infective endocarditis. A total of 101 infective endocarditis (IE) patients treated at Hangzhou First People's Hospital, from January 1, 2012, to April 1, 2022, were included in the present study. Baseline demographic data and laboratory data were collected for statistical analysis of risk factors and survival time in the IE with haemodialysis group (HD-IE group, n=15) and the IE without haemodialysis group (NHD-IE group, n=86). Haemoglobin, red blood cells, C-reactive protein, procalcitonin, serum albumin, diabetes, invasive procedures, positive blood bacteria culture, heart valve calcification ratio, and left ventricular ejection fraction level were risk factors for infective endocarditis complicated with haemodialysis (P<0.05). Compared with the NHD-IE group, the HD-IE group had an obviously increased risk of mortality (χ2=6.323, P=0.012). The univariate Cox regression analysis showed that age, haemoglobin, red blood cells, serum albumin, left ventricular ejection score, longest vegetation diameter, combined hypotension and diabetes were risk factors for death; furthermore, multivariate Cox regression showed that age (HR=1.187, P=0.015), combined hypotension (HR=0.921, P=0.025) and the longest vegetation diameter (HR=9.191, P=0.004) were independent risk factors affecting the survival of patients. Collectively, the present study revealed that the mortality rate of HD-IE patients was higher than that of NHD-IE patients. Older age, hypotension, and the longest vegetation diameter were independent risk factors affecting the survival of patients. For HD-IE patients, active and effective antibiotic treatment or surgical treatment should be strongly recommended.