Ultra-thin DLC diaphragm-based optical fiber sensor achieving a highly sensitive and broadband acoustic response.

In the design of an extrinsic Fabry-Perot interferometer (EFPI) acoustic sensor, broadband response and high-sensitivity sensing are usually conflicting and need to be carefully balanced. Here, we present a novel, to the best of our knowledge, optical fiber acoustic sensor based on an ultra-thin diamond-like carbon (DLC) film, fabricated using the plasma-enhanced chemical vapor deposition method, and transferred by a surface-energy-assisted method. The sensor exhibits a broadband response ranging from 200 Hz to 100 kHz, maintains an average sensitivity of 457.3 mV/Pa within the range of 6 to 30 kHz, and can detect weak acoustic signals down to 3.23 µPa/Hz1/2@16.19 kHz. The combination of an ultra-thin DLC film with a relatively high Young's modulus and internal stresses results in a trade-off between high sensitivity and a broadband response. This performance demonstrates that our sensor is among the most advanced in the EFPI acoustic sensor family, with significant potential for applications such as photoacoustic spectroscopy, defect diagnosis, and bio-imaging.

Long noncoding RNA BDNF-AS inversely regulated BDNF and modulated high-glucose induced apoptosis in human retinal pigment epithelial cells.

In this study, we characterized the functional role of long noncoding RNA (lncRNA), brain derived neurotrophic factor anti-sense (BDNF-AS) in regulating D-glucose-induced (DGI) apoptosis in human retinal pigment epithelial (RPE) cells. Human RPE cell line, ARPE-19 cells were cultured in vitro and treated with various concentrations of D-glucose for 24 h. A TUNEL assay was applied with immunohistochemical and quantitative approaches to assess the apoptotic effect of D-glucose. Under the condition of 50 mM D-glucose, qPCR was used to assess gene expression of BDNF and BDNF-AS in ARPE-19 cells. Using siRNA transfection, BDNF-AS was endogenously knocked down in ARPE-19 cells. The effects of BDNF-AS downregulation on DGI apoptosis and BDNF expression were assessed by TUNEL assay, qPCR, and Western blot, respectively. Furthermore, in BDNF-AS-downregulated ARPE-19 cells, secondary siRNA transfection was conducted to knock down endogenous BDNF expression. Its effect on BDNF-AS-associated apoptotic regulation was further evaluated. High concentrations of D-glucose induced significant apoptosis in ARPE-19 cells in vitro. With treatment of 50 mM D-glucose, BDNF was markedly downregulated whereas BDNF-AS upregulated in ARPE-19 cells. SiRNA-mediated BDNF-AS downregulation ameliorated DGI apoptosis and upregulated BDNF in ARPE-19 cells. In addition, inhibiting BDNF reversed the protective effect of BDNF-AS downregulation on DGI apoptosis. Our results suggest that BDNF-AS, through inverse regulation of BDNF, might play a critical role in the process of DGI apoptosis in diabetic retinopathy.

Deformation-Thermal Co-Induced Ferromagnetism of Austenite Nanocrystalline FeCoCr Powders for Strong Microwave Absorption.

Nanocrystalline soft magnetic alloy powders are promising microwave absorbents since they can work at diverse frequencies and are stable in harsh environments. However, when the alloy powders are in austenite phase, they are out of the screen for microwave absorbents due to their paramagnetic nature. In this work, we reported a strategy to enable strong microwave absorption in nanocrystalline austenite FeCoCr powders by deformation-thermal co-induced ferromagnetism via attritor ball milling and subsequent heat treatment. Results showed that significant austenite-to-martensite transformation in the FeCoCr powders was achieved during ball milling, along with the increase in shape anisotropy from spherical to flaky. The saturation magnetization followed parabolic kinetics during ball milling and rose from 1.43 to 109.92 emu/g after milling for 4 h, while it exhibited a rapid increase to 181.58 emu/g after subsequent heat treatment at 500 °C. A considerable increase in complex permeability and hence magnetic loss capability was obtained. With appropriate modulation of complex permittivity, the resultant absorbents showed a reflection loss of below -6 dB over 8~18 GHz at thickness of 1 mm and superior stability at 300 °C. Our strategy can broaden the material selection for microwave absorbents by involving Fe-based austenite alloys and simply recover the ferromagnetism of industrial products made without proper control of the crystalline phase.

A retrospective study of azithromycin and ceftizoxime for the management of children with Mycoplasma pneumoniae pneumonia.

ABSTRACT: The aim of this study was to compare the clinical efficacy of azithromycin and ceftizoxime (AC) and erythromycin and amoxicillin/sulbactam (EAS) in the treatment of children with Mycoplasma pneumoniae pneumonia (MPP).In this retrospective study, a total of 92 eligible children with MPP were included, and they were divided into a treatment group (n = 46) and a control group (n = 46). All patients were treated with intravenous ambroxol, and nebulized inhalation of budesonide and terbutaline. In addition, patients in the treatment group received AC. Patients in the control group underwent EAS. All patients in both groups were treated for a total of 10 days. Outcomes consist of erythrocyte sedimentation rate, C-reactive protein, serum lactate dehydrogenase, and interleukin 6, fever clearance time, time of cough disappearance, time of rale disappearance, time of signs disappeared by X-ray, and adverse events. All outcomes were measured after 10-day treatment.After treatment, patients who received AC exerted better improvements in erythrocyte sedimentation rate (P < .01), C-reactive protein (P < .01), serum lactate dehydrogenase (P < .01), interleukin 6 (P < .01), fever clearance time (P < .01), time of cough disappearance (P < .01), time of rale disappearance (P < .01), and time of signs disappeared by X-ray (P < .01), than those in patients who received EAS. In addition, there were not significant differences in adverse events between 2 groups.The results of this study showed that AC may benefit more than EAS for the children with MPP.

Naringenin promotes cell autophagy to improve high-fat-diet-induced atherosclerosis in ApoE-/- mice.

Naringenin (NAR) is a major flavanone in citrus fruits that has multiple pharmacological attributes such as anticancer and antiatherogenic. This study aims to investigate the mechanism of NAR in high-fat-diet (HFD)-induced atherosclerosis (AS) in apolipoprotein E-knockout (ApoE-/-) mice. A HFD-induced AS ApoE-/- mouse model was established. The mice were treated with HFD, different doses of NAR and simvastatin (Simv). After drug treatment, the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), and alanine aminotransferase (ALT) were determined. The expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was detected using qRT-PCR and enzyme-linked immunosorbent assay. The plaque area of the aorta of AS mice was determined using oil red O staining. Western blot analysis was applied to measure the levels of autophagy-related proteins [protein 1 light chain 3B (LC3B), beclin 1, and p62]. The TC, TG, LDL-C, TNF-α, ALT, and MDA levels were significantly increased while the HDL-C, SOD, and GSH-Px levels were decreased in the HFD-induced AS ApoE-/- mice. NAR treatment reversed the expression of the above indicators in mice. After they were treated with different doses of NAR, the LC3B and beclin 1 levels were improved while the p62 protein level was decreased. This study suggested that NAR could promote cell autophagy to improve HFD-induced AS in ApoE-/- mice.

High-throughput metabolomics for discovering metabolic biomarkers from intestinal tumorigenesis in APC min/+ mice based on liquid chromatography/mass spectrometry.

As a major public health concern, colon cancer is one of the most common cancer types, which is also the second cause of cancer death in developed countries and the third most common cancer in other parts of the world. It was reported that patients diagnosed at early stage have a chance to obtain 5-year survival rates at least compared to patients with late stage. Facing the multistep process in intestinal tumorigenesis, there is an urgent need to develop more effective early detection strategies for ameliorating the patient clinical outcome. Metabolomics open up a novel avenue of seeking valuable potential biomarkers for assessing disease severity and prognosticating course by dynamic snapshot of small molecule metabolites. The study aims to provide deeper insights into the discovery, identification and functional pathways analysis of differentially expressed metabolites in intestinal tumorigenesis in APC min/+ mice used by the serum metabolomics, and bring about useful information for further effective prevention and treatment of the disease. 17 marker metabolites and related metabolism pathway were identified using non-targeted metabolomics based on liquid chromatography/mass spectrometry (LC/MS) associated with multivariate statistical analysis. The ingenuity pathway analysis platform involved multiple-pathways was applied to metabolic network analysis for further understanding the relationship between functional metabolic pathways and disease.

Abatement of SF6 in the presence of NH3 by dielectric barrier discharge plasma.

In this paper, the degradation rate, energy yield and the degradation by-products of SF6 was studied when different concentrations of NH3 were added. When NH3 concentration increased from 0 to 2%, the degradation rate efficiency(DRE) of SF6 increased from 60% to 97.23% under the flow rate of 50ml/min and 94W input power. The energy yield(EY) reached 4.16g/kWh. In addition, we found that increasing the flow rate to 250ml/min, the DRE decreased to 58.71%, but the EY increased to 12.55g/kWh. The main gas by-products are SOF2, SO2F2, SO2, OF2, HF and NF3. When the concentration of initial NH3 increased, the SO2 concentration increased while the concentrations of SOF2, SO2F2, SOF4 decreased. In addition, we found that a pale yellow film formed on the surface of the reactor wall. XPS analysis showed that the solid products were mainly S, NH3HF and NH4HF2. The emission spectra show that NH3 addition can effectively promote the formation of active particles and increase plasma density.The addition of NH3 can convert some of the sulfur and fluorine into solid products and reduce the production of toxic gases.

Association of single-nucleotide polymorphisms in toll-like receptor 2 gene with asthma susceptibility: A meta-analysis.

BACKGROUND: An increasing number of studies have been carried out on the relationship between polymorphisms in toll-like receptor 2 (TLR2) gene and asthma risk. However, the results were controversial. With the purpose of yielding a more reliable estimation of the association, we conducted the present meta-analysis. METHODS: Multiple electronic databases up to August 22, 2016 were searched for literature retrieval. The association between the asthma susceptibility and the rs5743708 polymorphism, rs3804099 polymorphism, rs3804100 polymorphism, and rs4696480 polymorphism in TLR2 gene was appraised. The odds ratios (ORs) with 95% confidence intervals (CIs) under different genetic models were calculated. RESULTS: A total of 13 studies were eligible in our meta-analysis according to the predefined inclusion and exclusion criteria. There was no significant association between asthma risk and rs5743708, rs3804099, and rs3804100 polymorphisms in TLR2 gene under any genetic model. With respect to the TLR2 rs4696480 polymorphism, significant association was detected between asthma susceptibility and TLR2 rs4696480 polymorphism under dominant model (OR = 2.455, 95% CI = 1.235-4.88, P = .01) and codominant 3 model (OR = 2.776, 95% CI = 1.199-6.427,  = 0.017). CONCLUSIONS: Our meta-analysis reveals that the TLR2 rs4696480 polymorphism is significantly associated with asthma susceptibility, and the TLR2 rs4696480 polymorphism is a risk factor for asthma.

Association between polymorphisms in the promoter region of the apolipoprotein E (APOE) gene and Alzheimer's disease: A meta-analysis.

Several studies have evaluated the role of polymorphisms in the promoter region of APOE gene that encodes apolipoprotein E (APOE) and the susceptibility to Alzheimer's disease (AD). The aim of this literature review and meta-analysis was to investigate the relationship between the APOE promoter region single nucleotide polymorphisms (SNPs) (rs449647, -491A/T; rs769446, -427T/C and rs405509 -219T/G) and the risk of developing AD. Eligible controlled studies published up to November 2016 were retrieved from main online scientific and medical databases. Odds ratio (OR) and 95 % confidence interval (CI) were used to calculate the strength of the relationship. A total of 23 publications (19 for rs449647, ten for rs769446 and ten for rs405509) were retrieved that included 5,703 patients with AD and 5,692 controls. The C allele of the rs769446 variant of the promoter region of APOE gene was significantly associated with an increase of risk of AD (OR = 1.271, 95 % CI = 1.114-1.449, P < 0.0001), while other genetic models of this variant were not related with susceptibility to AD. Rs449647 and rs405509 polymorphisms of APOE gene were not associated with an increase of risk of AD. The findings of this literature review and meta-analysis have shown that rs769446 polymorphism in the promoter region of APOE gene could be a risk factor for AD. Future large-scale studies on the role of polymorphisms in the promoter region of APOE gene in AD are still awaited.

Naringenin promotes cell autophagy to improve high-fat-diet-induced atherosclerosis in ApoE-/- mice

Naringenin (NAR) is a major flavanone in citrus fruits that has multiple pharmacological attributes such as anticancer and antiatherogenic. This study aims to investigate the mechanism of NAR in high-fat-diet (HFD)-induced atherosclerosis (AS) in apolipoprotein E-knockout (ApoE-/-) mice. A HFD-induced AS ApoE-/- mouse model was established. The mice were treated with HFD, different doses of NAR and simvastatin (Simv). After drug treatment, the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (SOD), and alanine aminotransferase (ALT) were determined. The expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was detected using qRT-PCR and enzyme-linked immunosorbent assay. The plaque area of the aorta of AS mice was determined using oil red O staining. Western blot analysis was applied to measure the levels of autophagy-related proteins [protein 1 light chain 3B (LC3B), beclin 1, and p62]. The TC, TG, LDL-C, TNF-α, ALT, and MDA levels were significantly increased while the HDL-C, SOD, and GSH-Px levels were decreased in the HFD-induced AS ApoE-/- mice. NAR treatment reversed the expression of the above indicators in mice. After they were treated with different doses of NAR, the LC3B and beclin 1 levels were improved while the p62 protein level was decreased. This study suggested that NAR could promote cell autophagy to improve HFD-induced AS in ApoE-/- mice.