Separation of Mono-/Divalent Ions via Controlled Dynamic Adsorption/Desorption at Polythiophene Coated Carbon Surface with Flow-Electrode Capacitive Deionization.

Capacitive deionization for selective separation of ions is rarely reported since it relies on the electrostatic attraction of oppositely charged ions with no capability to distinguish ions of different valent states. Using molecular dynamic simulation, a screening process identified a hybrid material known as AC/PTh, which consists of activated carbon with a thin layer of polythiophene (PTh) coating. By utilizing AC/PTh as electrode material implementing the short-circuit cycle (SCC) mode in flow-electrode capacitive deionization (FCDI), selective separation of mono-/divalent ions can be realized via precise control of dynamic adsorption and desorption of mono-/divalent ions at a particular surface. Specifically, AC/PTh shows strong interaction with divalent ions but weak interaction with monovalent ions, the distribution of divalent ions can be enriched in the electric double layer after a couple of adsorption-desorption cycles. At Cu2+/Na+ molar ratio of 1:40, selectivity toward divalent ions can reach up to 110.3 in FCDI SCC mode at 1.0 V. This work presents a promising strategy for separating ions of different valence states in a continuously operated FCDI device.

Alterations of the bile microbiome is associated with progression-free survival in pancreatic ductal adenocarcinoma patients.

BACKGROUND: Patients with pancreatic ductal adenocarcinoma (PDAC) display an altered oral, gastrointestinal, and intra-pancreatic microbiome compared to healthy individuals. However, knowledge regarding the bile microbiome and its potential impact on progression-free survival in PDACs remains limited. METHODS: Patients with PDAC (n = 45), including 20 matched pairs before and after surgery, and benign controls (n = 16) were included prospectively. The characteristics of the microbiomes of the total 81 bile were revealed by 16  S-rRNA gene sequencing. PDAC patients were divided into distinct groups based on tumor marker levels, disease staging, before and after surgery, as well as progression free survival (PFS) for further analysis. Disease diagnostic model was formulated utilizing the random forest algorithm. RESULTS: PDAC patients harbor a unique and diverse bile microbiome (PCoA, weighted Unifrac, p = 0.038), and the increasing microbial diversity is correlated with dysbiosis according to key microbes and microbial functions. Aliihoeflea emerged as the genus displaying the most significant alteration among two groups (p < 0.01). Significant differences were found in beta diversity of the bile microbiome between long-term PFS and short-term PFS groups (PCoA, weighted Unifrac, p = 0.005). Bacillota and Actinomycetota were identified as altered phylum between two groups associated with progression-free survival in all PDAC patients. Additionally, we identified three biomarkers as the most suitable set for the random forest model, which indicated a significantly elevated likelihood of disease occurrence in the PDAC group (p < 0.0001). The area under the receiver operating characteristic (ROC) curve reached 80.8% with a 95% confidence interval ranging from 55.0 to 100%. Due to the scarcity of bile samples, we were unable to conduct further external verification. CONCLUSION: PDAC is characterized by an altered microbiome of bile ducts. Biliary dysbiosis is linked with progression-free survival in all PDACs. This study revealed the alteration of the bile microbiome in PDACs and successfully developed a diagnostic model for PDAC.

Regulating Surface Wettability and Charge Density of Porous Carbon Particles by In Situ Growth of Polyaniline for Constructing an Efficient Electrical Percolation Network in Flow-Electrode Capacitive Deionization.

Both electrical conductivity and surface wettability are required for the selection of active carbon materials in flow-electrode capacitive deionization, while a trade-off exists between these two properties. In this work, a hybrid material with a thin layer of polyaniline (PANI) coating on activated carbon (AC/PANI) was successfully developed to retain excellent electrical conductivity and acquire good surface wettability. By adjusting the dosage of initiator, AC/PANI composites with different loading fractions of PANI were obtained. The electrochemical testing demonstrated that the AC/PANI composites have higher specific capacitance and lower ion diffusion resistance compared to pure AC, resulting in better desalinization performance. Specifically, with a feed concentration of 1600 mg/L, excellent adsorption capacity and high charge efficiency can be simultaneously achieved at 13.51 mg/g and 92.21%, respectively. Benefiting from the formation of a continuous electrical percolation network and reduced solid/liquid interfacial transport resistance, a 39% enhancement of average salt adsorption rate (from 0.54 to 0.75 µmol/min/cm2) was obtained.

Comparison of UV-induced AOPs (UV/Cl2, UV/NH2Cl, UV/ClO2 and UV/H2O2 ) in the degradation of iopamidol: Kinetics, energy requirements and DBPs-related toxicity in sequential disinfection processes.

The UV-induced advanced oxidation processes (AOPs, including UV/Cl2, UV/NH2Cl, UV/ClO2 and UV/H2O2 ) degradation kinetics and energy requirements of iopamidol as well as DBPs-related toxicity in sequential disinfection were compared in this study. The photodegradation of iopamidol in these processes can be well described by pseudo-first-order model and the removal efficiency ranked in descending order of UV/Cl2  > UV/H2O2  > UV/NH2Cl > UV/ClO2  > UV. The synergistic effects could be attributed to diverse radical species generated in each system. Influencing factors of oxidant dosage, UV intensity, solution pH and water matrixes (Cl- , NH4 + and nature organic matter) were evaluated in detail. Higher oxidant dosages and greater UV intensities led to bigger pseudo-first-order rate constants (Kobs) in these processes, but the pH behaviors exhibited quite differently. The presence of Cl- , NH4 + and nature organic matter posed different effects on the degradation rate. The parameter of electrical energy per order (EE/O) was adopted to evaluate the energy requirements of the tested systems and it followed the trend of UV/ClO2  > UV > UV/NH2Cl > UV/H2O2  > UV/Cl2 . Pretreatment of iopamidol by UV/Cl2 and UV/NH2Cl clearly enhanced the production of classical disinfection by-products (DBPs) and iodo-trihalomethanes (I-THMs) during subsequent oxidation while UV/ClO2 and UV/H2O2 exhibited almost elimination effect. From the perspective of weighted water toxicity, the risk ranking was UV/NH2Cl > UV/Cl2 > UV > UV/H2O2 > UV/ClO2 . Among the discussed UV-driven AOPs, UV/Cl2 was proved to be the most cost-effective one for iopamidol removal while UV/ClO2 displayed overwhelming advantages in regulating the water toxicity associated with DBPs, especially I-THMs. The present results could provide some insights into the application of UV-activated AOPs technologies in tradeoffs between cost-effectiveness assessment and DBPs-related toxicity control of the disinfected waters containing iopamidol.

Black Phosphorus Accelerates Bone Regeneration Based on Immunoregulation.

A fundamental understanding of inflammation and tissue healing suggests that the precise regulation of the inflammatory phase, both in terms of location and timing, is crucial for bone regeneration. However, achieving the activation of early inflammation without causing chronic inflammation while facilitating quick inflammation regression to promote bone regeneration continues to pose challenges. This study reveals that black phosphorus (BP) accelerates bone regeneration by building an osteogenic immunological microenvironment. BP amplifies the acute pro-inflammatory response and promotes the secretion of anti-inflammatory factors to accelerate inflammation regression and tissue regeneration. Mechanistically, BP creates an osteoimmune-friendly microenvironment by stimulating macrophages to express interleukin 33 (IL-33), amplifying the inflammatory response at an early stage, and promoting the regression of inflammation. In addition, BP-mediated IL-33 expression directly promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), which further facilitates bone repair. To the knowledge, this is the first study to reveal the immunomodulatory potential of BP in bone regeneration through the regulation of both early-stage inflammatory responses and later-stage inflammation resolution, along with the associated molecular mechanisms. This discovery serves as a foundation for the clinical use of BP and is an efficient approach for managing the immune microenvironment during bone regeneration.

Algae polysaccharide-induced transport transformation of nanoplastics in seawater-saturated porous media.

This study examined the distinct effects of algae polysaccharides (AP), namely sodium alginate (SA), fucoidan (FU), and laminarin (LA), on the aggregation of nanoplastics (NP) in seawater, as well as their subsequent transport in seawater-saturated sea sand. The pristine 50 nm NP tended to form large aggregates, with an average size of approximately 934.5 ± 11 nm. Recovery of NP from the effluent (Meff) was low, at only 18.2 %, and a ripening effect was observed in the breakthrough curve (BTC). Upon the addition of SA, which contains carboxyl groups, the zeta (ζ)-potential of the NP increased by 2.8 mV. This modest enhancement of electrostatic interaction with NP colloids led to a reduction in the aggregation size of NP to 598.0 ± 27 nm and effectively mitigated the ripening effect observed in the BTC. Furthermore, SA's adherence to the sand surface and the resulting increase in electrostatic repulsion, caused a rise in Meff to 27.5 %. In contrast, the introduction of FU, which contains sulfate ester groups, resulted in a surge in ζ-potential of the NP to -27.7 ± 0.76 mV. The intensified electrostatic repulsion between NP and between NP and sand greatly increased Meff to 45.6 %. Unlike the effects of SA and FU, the addition of LA, a neutral compound, caused a near disappearance of ζ-potential of NP (-3.25 ± 0.68 mV). This change enhanced the steric hindrance effect, resulting in complete stabilization of particles and a blocking effect in the BTC of NP. Quantum chemical simulations supported the significant changes in the electrostatic potential of NP colloids induced by SA, FU and LA. In summary, the presence of AP can induce variability in the mobility of NP in seawater-saturated porous media, depending on the nature of the weak, strong, or non-electrostatic interactions between colloids, which are influenced by the structure and functionalization of the polysaccharides themselves. These findings provide valuable insights into the complex and variable behavior of NP transport in the marine environment.

Time-course swRNA-seq uncovers a hierarchical gene regulatory network in controlling the response-repair-remodeling after wounding.

Wounding initiates intricate responses crucial for tissue repair and regeneration. Yet, the gene regulatory networks governing wound healing remain poorly understood. Here, employing single-worm RNA sequencing (swRNA-seq) across 12 time-points, we delineated a three-stage wound repair process in C. elegans: response, repair, and remodeling. Integrating diverse datasets, we constructed a dynamic regulatory network comprising 241 transcription regulators and their inferred targets. We identified potentially seven autoregulatory TFs and five cross-autoregulatory loops involving pqm-1 and jun-1. We revealed that TFs might interact with chromatin factors and form TF-TF combinatory modules via intrinsically disordered regions to enhance response robustness. We experimentally validated six regulators functioning in transcriptional and translocation-dependent manners. Notably, nhr-76, daf-16, nhr-84, and oef-1 are potentially required for efficient repair, while elt-2 may act as an inhibitor. These findings elucidate transcriptional responses and hierarchical regulatory networks during C. elegans wound repair, shedding light on mechanisms underlying tissue repair and regeneration.

Activation of the osteoblastic HIF-1α pathway partially alleviates the symptoms of STZ-induced type 1 diabetes mellitus via RegIIIγ.

The hypoxia-inducible factor-1α (HIF-1α) pathway coordinates skeletal bone homeostasis and endocrine functions. Activation of the HIF-1α pathway increases glucose uptake by osteoblasts, which reduces blood glucose levels. However, it is unclear whether activating the HIF-1α pathway in osteoblasts can help normalize glucose metabolism under diabetic conditions through its endocrine function. In addition to increasing bone mass and reducing blood glucose levels, activating the HIF-1α pathway by specifically knocking out Von Hippel‒Lindau (Vhl) in osteoblasts partially alleviated the symptoms of streptozotocin (STZ)-induced type 1 diabetes mellitus (T1DM), including increased glucose clearance in the diabetic state, protection of pancreatic ß cell from STZ-induced apoptosis, promotion of pancreatic ß cell proliferation, and stimulation of insulin secretion. Further screening of bone-derived factors revealed that islet regeneration-derived protein III gamma (RegIIIγ) is an osteoblast-derived hypoxia-sensing factor critical for protection against STZ-induced T1DM. In addition, we found that iminodiacetic acid deferoxamine (SF-DFO), a compound that mimics hypoxia and targets bone tissue, can alleviate symptoms of STZ-induced T1DM by activating the HIF-1α-RegIIIγ pathway in the skeleton. These data suggest that the osteoblastic HIF-1α-RegIIIγ pathway is a potential target for treating T1DM.

Satellite record reveals 1960s acceleration of Totten Ice Shelf in East Antarctica.

Wilkes Land and Totten Glacier (TG) in East Antarctica (EA) have been losing ice mass significantly since 1989. There is a lack of knowledge of long-term mass balance in the region which hinders the estimation of its contribution to global sea level rise. Here we show that this acceleration trend in TG has occurred since the 1960s. We reconstruct ice flow velocity fields of 1963-1989 in TG from the first-generation satellite images of ARGON and Landsat-1&4, and build a five decade-long record of ice dynamics. We find a persistent long-term ice discharge rate of 68 ± 1 Gt/y and an acceleration of 0.17 ± 0.02 Gt/y2 from 1963 to 2018, making TG the greatest contributor to global sea level rise in EA. We attribute the long-term acceleration near grounding line from 1963 to 2018 to basal melting likely induced by warm modified Circumpolar Deep Water. The speed up in shelf front during 1973-1989 was caused by a large calving front retreat. As the current trend continues, intensified monitoring in the TG region is recommended in the next decades.

Urchin-like ceria nanoparticles for enhanced gene therapy of osteoarthritis.

Osteoarthritis (OA) is the most common degenerative joint disease in the world. Gene therapy based on delivering microRNAs (miRNAs) into cells has potential for the treatment of OA. However, the effects of miRNAs are limited by the poor cellular uptake and stability. Here, we first identify a type of microRNA-224-5p (miR-224-5p) from clinical samples of patients with OA that can protect articular cartilage from degeneration and further synthesize urchin-like ceria nanoparticles (NPs) that can load miR-224-5p for enhanced gene therapy of OA. Compared with traditional sphere ceria NPs, the thorns of urchin-like ceria NPs can efficiently promote the transfection of miR-224-5p. In addition, urchin-like ceria NPs have excellent performance of scavenging reactive oxygen species (ROS), which can regulate the microenvironment of OA to further improve the gene treatment of OA. The combination of urchin-like ceria NPs and miR-224-5p not only exhibits favorable curative effect for OA but also provides a promising paradigm for translational medicine.

Performance evaluation of the UV activated chlorite process on trimethoprim: Degradation efficiency, energy consumption and disinfection by-products formation.

As the primary inorganic by-product species of ClO2, chlorite is believed to have negative toxicological effects on human health and therefrom greatly limits the wide application of ClO2 in water treatment. The synergistic trimethoprim (TMP) removal concerning degradation efficiency, energy consumption and disinfection by-products (DBPs) formation in the UV activated chlorite process accompanied by the simultaneously elimination of chlorite was comprehensively evaluated. UV/chlorite integrated process removed TMP far more rapidly than UV (1.52%) or chlorite (3.20%) alone due to the endogenous radicals (Cl•, ClO• and •OH), the contributing proportions of which were 31.96%, 19.20% and 44.12%. The second-order rate constants of TMP reaction with Cl•, ClO• and •OH were determined to be 1.75 × 1010, 1.30 × 109 and 8.66 × 109 M-1 s-1. The effects of main water parameters including chlorite dosage, UV intensity, pH as well as water matrixes (nature organic matter, Cl- and HCO3-) were examined. kobs obeyed the order as UV/Cl2>UV/H2O2≈UV/chlorite>UV, and the cost ranking via electrical energy per order (EE/O, kWh m-3 order-1) parameter was UV/chlorite (3.7034) > UV/H2O2 (1.1625) >UV/Cl2 (0.1631). The operational scenarios can be optimized to achieve the maximum removal efficiencies and the minimum energy costs. The destruction mechanisms of TMP were proposed by LC-ESI-MS analysis. The overall weighted toxicity in subsequent disinfection was assessed as UV/Cl2>UV/chlorite > UV, the values of which in post-chlorination were 6.2947, 2.5806 and 1.6267, respectively. Owing to the vital roles of reactive chlorine species (RCS), UV/chlorite displayed far higher TMP degradation efficiency than UV, and concurrently presented much less toxicity than UV/Cl2. In an effort to determine the viability of the promising combination technology, this study was devoted to reduce and reuse chlorite and synchronously realize the contaminants degradation efficiently.

Several carbon-coated Ga2O3 anodes: efficient coating of reduced graphene oxide enhanced the electrochemical performance of lithium ion batteries.

Gallium oxide as a novel electrode material has attracted attention because of its high stability and conductivity. In addition, Ga2O3 will be converted to Ga during the charge and discharge process, and the self-healing behavior of Ga can improve the cycling stability. In this paper, we synthesized Ga2O3 nanoparticles with a size of about 4 nm via a facile sol-gel method. Meanwhile, we employed three types of carbon materials (reduced graphene oxide, mesoporous carbon nanofiber arrays, and carbon nanotubes) to avoid the aggregation of Ga2O3 nanoparticles and improve the conductivity of Ga2O3 during the discharge/charge process as well. Among the three samples, the deactivating defective sites and special carbon matrix of reduced graphene oxide can provide more attachment points for Ga ions, so the Ga2O3 nanoparticles can be more closely and uniformly distributed on rGO. Benefitting from the perfect combination of reduced graphene oxide sheets and Ga2O3 nanoparticles, a stable capacity of the Ga2O3/rGO electrode can be maintained at 411 mA h g-1 at a current density of 1000 mA g-1 after 600 cycles. We believe that this work provides a novel and efficient way to improve the electrochemical stability of Li-ion batteries.

One-step synthesis of MOF-derived Ga/Ga2O3@C dodecahedra as an anode material for high-performance lithium-ion batteries.

A Ga/Ga2O3@C dodecahedron composite with a high specific capacity of about 542 mA h g-1 after 200 cycles at the current density of 1000 mA g-1 was synthesized by one-step hydrogen reduction. This hierarchical homogeneous structure combined the Ga, Ga2O3 and carbon frameworks (from Ga-MOF) to exhibit excellent electrochemical performance.

A novel Zr-MOF-based and polyaniline-coated UIO-67@Se@PANI composite cathode for lithium-selenium batteries.

In this work, we synthesized a novel UIO-67@Se@PANI composite cathode material for Li-Se battery applications. Zr-MOFs (metal organic frameworks) were used as a support and a PANI (polyaniline) layer was employed as the coating. UIO-67@Se@PANI was expected to be one of the candidates for Li-Se batteries, with a high specific capacity of 248.3 mA h g-1 at 1C (1C = 675 mA g-1) after 100 cycles. In particular, stable capacities of 203.1 and 167.6 mA h g-1 were received after 100 cycles at high rates of 2C and 5C, respectively. To explain such a good electrochemistry performance of the composite cathode material, multiple characterization methods were carried out. And that can be attributed to the sandwich-like structure of the UIO-67@Se@PANI composite and the fact that UIO-67 can provide unsaturated sites to tether the selenium effectively and the PANI layer can improve the electronic conductivity of the whole electrode significantly.

Bi2S3/C nanorods as efficient anode materials for lithium-ion batteries.

Bi2S3 is a promising negative electrode material for lithium storage owing to its high theoretical capacity. Nevertheless, the capacity of Bi2S3 decays very rapidly upon Li cycling. Here, Bi2S3 and Bi2S3/C were successfully synthesized by a novel route. Sulfur powder as a kind of sulfur source reacted with a metal organic framework based on bismuth and trimesinic acid-Bi(BTC)(DMF)·DMF·(CH3OH)2 (denoted as Bi-BTC). Trimesic acid further acted as a new type of carbon source to synthesize the Bi2S3/C composite. The particle sizes of the composite were smaller than those of pure Bi2S3, showing the suppression of Bi2S3 aggregation. Charge-discharge performance and cyclability for both the Bi2S3 and Bi2S3/C composites in lithium-ion batteries were measured. Specifically, the specific capacities of Bi2S3/C and Bi2S3 reached 765 and 603 mA h g-1, respectively, at 100 mA g-1 after 100 cycles. Because of its high capacity and excellent cycle life, Bi2S3/C is a promising energy storage material.