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1.
J Basic Microbiol ; 55(8): 992-1001, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25847583

RESUMO

Nematophagous fungi are considered to have the best potential as biological agents for the control of gastrointestinal nematodes in domestic animals. However, relatively few studies have been conducted with the genus Monacrosporium, especially with strains native to China. In the present study, we isolated and identified nematophagous fungi from fresh sheep feces. A pure fungal strain was molecularly characterized, and its nematophagous activity was evaluated. The morphological plasticity of the isolated strain, as well as its interaction with the nematode targets, was observed by scanning electron microscopy of the infected Trichostrongylus colubriformis L3 and the free-living nematode Caenorhabditis elegans. Three isolated fungal strains from the 30 fresh fecal samples of sheep from Inner Mongolia, China exhibited predatory activity; however, only a single strain was successfully purified (SF 0459). The SF 0459 strain was characterized by morphological analysis of its conidia and sequencing of its ITS1-5.8S rDNA-ITS2 region. This strain was identified to be Monacrosporium salinum (GenBank ID: KP036623). Nematophagous fungus helper bacteria were found at the interaction points between fungi and nematodes. The percentage of live T. colubriformis L3 was reduced by 83.79-88.69% based on the in vitro assay.


Assuntos
Ascomicetos/isolamento & purificação , Ascomicetos/fisiologia , Caenorhabditis elegans/microbiologia , Fezes/microbiologia , Trichostrongylus/microbiologia , Animais , Ascomicetos/classificação , Ascomicetos/citologia , China , Microscopia Eletrônica de Varredura , Dados de Sequência Molecular , Controle Biológico de Vetores , Filogenia , Análise de Sequência de DNA , Ovinos , Esporos Fúngicos/isolamento & purificação , Esporos Fúngicos/ultraestrutura
2.
J Parasitol ; 101(4): 476-84, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25978186

RESUMO

The nematophagous fungus Duddingtonia flagrans has been investigated as a biological agent for the control of gastrointestinal nematodes infecting domestic animals in other countries. However, D. flagrans has not been detected in China. In this study 1,135 samples were examined from 2012 to 2014; 4 D. flagrans isolates (SDH 035, SDH 091, SFH 089, SFG 170) were obtained from the feces of domestic animals and dung compost. The 4 isolates were then characterized morphologically. The SDH 035 strain was characterized by sequencing the ITS1-5.8S rDNA-ITS2 region. A BLAST search showed that the SDH 035 strain (GenBank KP257593) was 100% identical to Arthrobotrys flagrans (AF106520) and was identified as D. flagrans. The morphological plasticity of the isolated strain and the interaction of this strain with the nematode targets were observed by subjecting the infected trichostrongylide L3 to scanning electron microscopy. At 6 and 8 hr after trichostrongylide L(3) was added, hyphal ramifications were observed and L(3) were captured, respectively. Scanning electron micrographs were obtained at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr, where 0 is the time when trichostrongylide L(3) were first captured by the fungus. The details of the capture process by the fungus are also described. Chlamydospores were observed in the body of L(3) in the late stage of digestion. A sticky substance and bacteria could be observed in contact areas between predation structures and nematode cuticle.


Assuntos
Doenças dos Bovinos/prevenção & controle , Duddingtonia/isolamento & purificação , Doenças dos Ovinos/prevenção & controle , Trichostrongyloidea/microbiologia , Tricostrongiloidíase/veterinária , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/parasitologia , China , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Ribossômico/química , Duddingtonia/fisiologia , Duddingtonia/ultraestrutura , Fezes/microbiologia , Interações Hospedeiro-Patógeno , Larva/microbiologia , Microscopia Eletrônica de Varredura/veterinária , Dados de Sequência Molecular , Análise de Sequência de DNA/veterinária , Ovinos , Doenças dos Ovinos/parasitologia , Microbiologia do Solo , Tricostrongiloidíase/prevenção & controle
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