RESUMO
AIM: Renal fibrosis is a common cause of renal dysfunction with chronic kidney diseases. This process is characterized by excessive production of extracellular matrix (ECM) or inhibition of ECM degradation. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) proteinases, which are widely presented in mammals, have very critical roles in ECM remodelling. We aimed to study the role of ADAMTS proteinases and some of the ECM markers in the pathogenesis of renal fibrosis and to investigate the effects of hypoxia on these biomarkers. METHODS: In addition to the control group, Adriamycin (ADR) treated rats were divided into four groups as ADR, sham and two hypoxia groups. Renal nephropathy was assessed biochemical assays, pathological and immunohistochemical staining methods. The expression of ADAMTSs and mRNA were determined using Western blotting and real-time PCR, respectively. RESULTS: Renal dysfuntion and tissue damage in favour of ECM accumulation and renal fibrosis were observed in the ADR group. This was approved by remarkable changes in the expression of ADAMTS such as increased ADAMTS-1, -12 and -15. In addition, it was found that hypoxia and duration of hypoxia enhanced markers of tubulointerstitial fibrosis in the rat kidney tissues. Also, expression differences especially in ADAMTS-1, -6 and -15 were observed in the hypoxia groups. The variable and different expression patterns of ADAMTS proteinases in the ADR-induced renal fibrosis suggest that ADAMTS family members are involved in the development and progression of fibrosis. CONCLUSION: The expression changes of ADAMTS proteinases in kidney and association with hypoxia have potential clues to contribute to the early diagnosis and treatment options of renal fibrosis.
Assuntos
Proteínas ADAMTS/análise , Matriz Extracelular/química , Rim/patologia , Animais , Biomarcadores/análise , Hipóxia Celular , Modelos Animais de Doenças , Doxorrubicina/administração & dosagem , Fibrose/induzido quimicamente , Rim/química , Masculino , Ratos , Ratos WistarRESUMO
OBJECTIVE: This study is aimed to investigate the efficacy of erdosteine usage in acetaminophen-induced liver damage and to compare it with N-acetyl cysteine (NAC) in the treatment and prevention of liver toxicity due to overdose of acetaminophen. METHODS: The rats were separated into the following six groups of seven rats each: control group; acetaminophen (1 g/kg, orally); acetaminophen (1 g/kg, orally) + erdosteine (150 mg/kg/day, orally); acetaminophen (1 g/kg, orally) + NAC (140 mg/kg loading dose, followed by 70 mg/kg, orally); NAC (140 mg/kg loading dose, followed by 70 mg/kg, orally); erdosteine (150 mg/kg/kg, orally), subsequently. In all the groups, potential liver injuries were evaluated using biochemical and hematological analyses, oxidant-antioxidant parameters and histopathological parameters. RESULTS: In acetaminophen-treated group, levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total oxidant status (TOS) in the blood, prothrombin time (PT) and international normalized ratio (INR) were significantly increased when compared with controls. However, total antioxidant capacity (TAC) and glutathione (GSH) levels were decreased in group treated with acetaminophen, when compared with control group. Levels of AST, ALT and TOS, PT and INR were decreased in groups treated with NAC and erdosteine after acetaminophen administration, but the levels of TAC and GSH were increased. Histopathological improvements were observed in the groups treated with NAC and erdosteine after acetaminophen administration. CONCLUSION: The present study demonstrated that, in the prevention of liver damage induced by acetaminophen intoxication, an early treatment with a single dose of erdosteine was beneficial instead of NAC administration.
Assuntos
Acetaminofen/toxicidade , Acetilcisteína/uso terapêutico , Antídotos/uso terapêutico , Antipiréticos/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Tioglicolatos/uso terapêutico , Tiofenos/uso terapêutico , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Feminino , Coeficiente Internacional Normatizado , Oxidantes/sangue , Tempo de Protrombina , Ratos , Ratos WistarRESUMO
The aim of this study was to investigate the relationship of AEG-1 and p53 with the prognostic parameters of renal cell carcinoma (RCC). In this study, 50 paraffin blocks were histopathologically diagnosed at the Department of Pathology of the Medical Hospital of Duzce University, between 2005 and 2011. The cases consisted of 24 clear cell (CC) and 26 non-clear cell (NCC) RCC subtypes as follows: 24 (48%) clear cell RCC, 12 (24%) papillary RCC, 4 (8%) multilocular cystic RCC and 10 (20%) chromophobe RCC; none had sarcomatoid changes. By immunohistochemical analysis we investigated AEG-1 and p53 expression in carcinomas of the kidney, and by statistical analysis determined their relationship with clinicopathological parameters. Significant relationships were found between increasing tumor diameter and the increase of p53 (p = 0.028). In addition, p53 was significantly related to renal sinus invasion (p = 0.05) and Fuhrman grade (p = 0.026). There was a significant relationship between increased AEG-1 staining scores and CC and NCC carcinoma subtypes (p = 0.032), tumor capsule invasion (p = 0.01) and lymphovascular invasion (p = 0.015). There was also a significant correlation between tumor size and capsule and lymphovascular invasion (p = 0.02). We concluded that high AEG-1 and p53 expression correlates with the prognostic parameters in RCC patients, and in addition may be associated with tumor progression.
Assuntos
Carcinoma de Células Renais/patologia , Moléculas de Adesão Celular/metabolismo , Neoplasias Renais/patologia , Proteína Supressora de Tumor p53/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Renais/metabolismo , Núcleo Celular/metabolismo , Feminino , Perfilação da Expressão Gênica , Humanos , Imuno-Histoquímica , Rim/metabolismo , Rim/patologia , Neoplasias Renais/metabolismo , Masculino , Proteínas de Membrana , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Prognóstico , Proteínas de Ligação a RNARESUMO
BACKGROUND: We evaluated the effect of calcium alginate as a hemostatic agent in a splenic injury model. METHODS: Experimental rats (Wistar albino) were divided into four groups. Group I: Laparotomy was not performed. Group II: After laparotomy, the abdomen was closed without any splenic injury. Group III: After laparotomy, splenic injury about 0.5 cm in depth and 0.3 cm in length was created by standard Rochester pean forceps. Physiological serum treated gauze dressing, about 2x2 cm in size, was applied to the injured splenic tissue for 3 minutes. Group IV: After laparotomy, standard splenic injury about 0.5 cm in length and 0.3 cm in depth was created. Calcium alginate wound dressing, 1x1 cm in size, was applied to the splenic wound. In all groups, blood samples for bleeding time and hemogram were taken. Peroperative blood loss, pre- and post-operative hemoglobin and hematocrit values were calculated. RESULTS: Comparing hematocrit values and peroperative bleeding in Groups III and IV, Group IV had a lower decline in hematocrit values and lower peroperative bleeding. CONCLUSION: Calcium alginate has hemostatic capacity. It may be used in splenic injuries, especially for Grades I and II.
Assuntos
Traumatismos Abdominais/tratamento farmacológico , Alginatos/farmacologia , Hemorragia/tratamento farmacológico , Hemorragia/prevenção & controle , Hemostáticos/farmacologia , Baço/lesões , Traumatismos Abdominais/sangue , Animais , Bandagens , Modelos Animais de Doenças , Ácido Glucurônico/farmacologia , Hematócrito , Ácidos Hexurônicos/farmacologia , Masculino , Ratos , Ratos WistarRESUMO
Odontomas are benign tumors of odontogenic origin. The cause of the odontoma is unknown, but it is believed to be hereditary or due to a disturbance in tooth development triggered by trauma or infection. Odontomas may be either compound or complex. Although these tumors are seen frequently, erupted odontomas are rare. The purpose of this study is to present a rare case of complex odontoma that erupted into the oral cavity.
Assuntos
Neoplasias Maxilares/patologia , Odontoma/patologia , Cemento Dentário/patologia , Esmalte Dentário/patologia , Dentina/patologia , Feminino , Humanos , Adulto JovemRESUMO
Pigmented villonodular synovitis (PVS) is a benign proliferative disorder of unknown origin that affects synovial joints, most commonly the knee. The joint knee can be affected by localized or diffuse form. Diffuse PVS, the aggressive form of the disease is much more problematic, especially when it extends extra-articularly, and is associated with high recurrence rates. Although this disease is categorized as an inflammatory process rather than a neoplasm, it may be locally destructive and involve muscles, tendons, bone and skin. Neural involvement of the disease is rather rare, and only limited knowledge about neuropathy due to PVS we have yet. The presentation of the disease in our patient is a peroneal neuropathy which is the first reported case in English language literature of PVS of the knee seen with extra-articular tissue involvement.
Assuntos
Neuropatias Fibulares/diagnóstico , Neuropatias Fibulares/etiologia , Sinovite Pigmentada Vilonodular/complicações , Sinovite Pigmentada Vilonodular/diagnóstico , Humanos , Articulação do Joelho/patologia , Articulação do Joelho/cirurgia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Procedimentos Ortopédicos/métodos , Nervo Fibular/patologia , Nervo Fibular/cirurgia , Neuropatias Fibulares/cirurgia , Sinovite Pigmentada Vilonodular/cirurgiaRESUMO
Lipedematous scalp is characterized by increased subcutaneous thickness of the scalp without any hair change. A 50-year-old female presented with thickening of the scalp without alopecia. She had no disorder other than hypertension. Monthly sonographic measurements without any treatment showed a resolution tendency in some areas and an increased thickness in others. We discuss the characteristics of this rare and possibly underestimated entity, including differential diagnoses.
Assuntos
Edema/diagnóstico , Dermatoses do Couro Cabeludo/diagnóstico , Edema/etiologia , Edema/terapia , Feminino , Humanos , Pessoa de Meia-Idade , Dermatoses do Couro Cabeludo/etiologia , Dermatoses do Couro Cabeludo/terapiaRESUMO
Melkersson-Rosenthal syndrome is a complex neuromucocutaneous disorder. A 60-year-old woman presented with granulomatous cheilitis of the lower lip, unilateral facial paralysis, and ipsilateral facial and acral swelling. The result of histopathological evaluation in extremities and inferior lip was compatible with Melkersson-Rosenthal syndrome.
Assuntos
Edema/etiologia , Síndrome de Melkersson-Rosenthal/diagnóstico , Biópsia , Face , Feminino , Pé , Mãos , Histiócitos/patologia , Humanos , Linfócitos/patologia , Pessoa de Meia-Idade , Plasmócitos/patologia , Pele/patologiaRESUMO
Cutaneous metastasis of prostate cancer is rare. Lesions in this type of skin metastasis are usually seen as suprapubic nodules. Here, we presented two cases with skin metastases of prostate cancer characterized by grouped cutaneous and subcutaneous nodules.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias de Próstata Resistentes à Castração/patologia , Neoplasias Cutâneas/secundário , Idoso , Biópsia , Evolução Fatal , Humanos , Calicreínas/sangue , Masculino , Antígeno Prostático Específico/sangue , Neoplasias de Próstata Resistentes à Castração/sangue , Neoplasias de Próstata Resistentes à Castração/terapia , Pele/patologia , Neoplasias Cutâneas/tratamento farmacológico , Neoplasias Cutâneas/patologiaRESUMO
AIM: To evaluate the protective effect of l-carnitine on liver tissue preserved in University of Wisconsin (UW) solution. METHODS: Twenty Wistar Albino rats were divided into two groups, a control (UW) group and a UW plus l-carnitine group. Retrieved liver grafts were preserved in UW and UW plus l-carnitine solutions at +4 degrees C. Preservation solution samples were assessed at 2, 24, 36, and 48 h to measure alanine aminotransferase and acid phosphatase activity. Tissue injury was scored on paraffin sections. RESULTS: No micro or macrovacuolar fat droplets were observed in the tissue slices. l-Carnitine effectively decreased enzyme release when added to UW solution (P < 0.05). CONCLUSION: In addition to fatty liver, l-carnitine might be a metabolic adjunct in preservation solutions for non-fatty liver within UW solution.
Assuntos
Equinococose/diagnóstico por imagem , Parede Torácica/diagnóstico por imagem , Idoso , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Equinococose/tratamento farmacológico , Equinococose/cirurgia , Echinococcus granulosus/efeitos dos fármacos , Feminino , Humanos , Radiografia , Parede Torácica/cirurgia , Resultado do TratamentoRESUMO
Spinal schwannomas are benign tumors arising from spinal nerve root sheaths. These are the most common intradural extramedullary spinal tumors. Schwannomas are mostly solid or heterogeneous solid tumors. Cystic schwannomas are rare lesions. We present a 27-year-old woman with an intradural extramedullary cystic tumor in the lower thoracic region who complained of back pain and walking difficulty. The patient was operated and the tumor was totally removed. The postoperative course was uneventful. The histopathological diagnosis was cystic schwannoma. Differentiation of cystic schwannomas from other cystic mass lesions can be difficult. Magnetic resonance imaging and histopathological findings are important for evaluating these tumors.
Assuntos
Neurilemoma/patologia , Neoplasias do Sistema Nervoso Periférico/patologia , Raízes Nervosas Espinhais/patologia , Vértebras Torácicas , Adulto , Biópsia , Feminino , Humanos , Imageamento por Ressonância Magnética , Neurilemoma/cirurgia , Neoplasias do Sistema Nervoso Periférico/cirurgia , Raízes Nervosas Espinhais/cirurgiaRESUMO
AIM: To confirm the usefulness of blood pool scintigraphy with Tc-labelled red blood cells ((99m)Tc-RBCs) in the diagnosis and follow-up of juvenile nasopharyngeal angiofibroma. METHODS: A prospective study design was used. (99m)Tc-RBCs were prepared by a modified in-vivo method. After the rapid intravenous injection of 370-740 MBq of (99m)Tc-RBCs, dynamic imaging of 1-min duration was performed. After dynamic imaging, static acquisitions at 5 min (second phase: blood pool phase) and 2 h (third phase: static image) were obtained. In addition, single-photon emission computed tomography (SPECT) imaging was performed at 2 h. SPECT images were obtained using a rotating gamma camera (GE-Starcam 4000 XR/T). RESULTS: All patients showed no activity in the first phase and mild activity in the second phase (blood pool phase). All patients with juvenile nasopharyngeal angiofibroma showed a prominent increased activity in the third phase and in SPECT images. CONCLUSIONS: This study shows that blood pool scintigraphy with (99m)Tc-RBC SPECT is very accurate, easy to perform and a suitable alternative to pre-operative and post-operative imaging techniques, including computed tomography scan, magnetic resonance imaging (MRI) and MRI angiography, for the detection of juvenile nasopharyngeal angiofibroma.
Assuntos
Angiofibroma/diagnóstico por imagem , Eritrócitos/diagnóstico por imagem , Neoplasias Nasofaríngeas/diagnóstico por imagem , Tecnécio , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Adolescente , Angiofibroma/irrigação sanguínea , Criança , Feminino , Seguimentos , Humanos , Masculino , Neoplasias Nasofaríngeas/irrigação sanguínea , Compostos Radiofarmacêuticos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Schwannomas (neurilemmomas) are benign neurogenic tumours of peripheral nerves. They originate from Schwann cells, which form the neural sheath. Although Schwannomas and neurofibromas are the most common primary peripheral nerve tumours, Schwannomas are rarely observed in ophthalmic areas. When they occur, ocular Schwannomas are usually located in the orbit, uveal tract and conjunctiva. Isolated eyelid Schwannomas are reported infrequently. Herein, we describe a case of eyelid Schwannoma in a 50-year-old man. The diagnosis of Schwannoma was made after the eyelid mass was removed by excisional biopsy, so this entity should be included in the differential diagnosis of eyelid margin tumours.
Assuntos
Doenças do Ânus/virologia , Carcinoma Verrucoso/virologia , Condiloma Acuminado/virologia , Neoplasias dos Genitais Masculinos/virologia , Papillomavirus Humano 6/isolamento & purificação , Infecções por Papillomavirus/complicações , Escroto , Doenças do Ânus/patologia , Doenças do Ânus/cirurgia , Carcinoma Verrucoso/patologia , Carcinoma Verrucoso/cirurgia , Condiloma Acuminado/patologia , Condiloma Acuminado/cirurgia , Neoplasias dos Genitais Masculinos/patologia , Neoplasias dos Genitais Masculinos/cirurgia , Papillomavirus Humano 11/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/cirurgia , Escroto/virologia , Resultado do TratamentoRESUMO
Syringomas are benign tumors derived from the intraepidermal portion of eccrine sweat ducts. They usually occur on the periobital area, but have also been found on the scalp, forehead, cheeks, axillae, abdomen, extremities, genitalia, and buttocks. We describe a patient with an unusual presentation of unilateral syringoma of the face associated with hyperthyroidism.
Assuntos
Neoplasias Faciais/patologia , Hipertireoidismo/diagnóstico , Neoplasias das Glândulas Sudoríparas/patologia , Siringoma/patologia , Adulto , Biópsia por Agulha , Neoplasias Faciais/complicações , Feminino , Humanos , Hipertireoidismo/complicações , Imuno-Histoquímica , Imageamento por Ressonância Magnética , Prognóstico , Medição de Risco , Índice de Gravidade de Doença , Neoplasias das Glândulas Sudoríparas/complicações , Siringoma/complicações , Testes de Função TireóideaRESUMO
A 68-year-old woman with progressive visual loss and exophthalmos in her right eye had been operated on for a mass in her right calf 3 years earlier. Imaging showed a huge mass invading the orbital structures and temporal pole. The presumptive diagnosis was a malignant orbital tumor. The tumor was resected totally and eroded tissues such as the lateral rectus muscle and dural compartments were repaired. The histological diagnosis was a malignant peripheral nerve sheath tumor (MPNST). The patient recovered uneventfully and was discharged 8 days after surgery. Two years later she died from a liver tumor. Few MPNSTs involving the orbit have been reported.
RESUMO
Castleman's disease (CD), also known as angiofollicular lymph node hyperplasia, is an uncommon, lymphoproliferative disorder of unknown etiology, mostly involving the mediastinum. Parenchymal lung involvement of the disease is extremely rare. Intrapulmonary CD has been reported in seven cases in the English literature. We describe an asymptomatic 28-year-old woman with lesion in the chest X-ray. Computed tomography (CT) of the chest confirmed a 5.5 × 5 cm well-defined, lobulated mass in the hilum of the right upper lobe. She underwent surgical resection for diagnosis and treatment. Pathologic examination showed hyaline vascular type (Castleman's disease) lymph node hyperplasia. CD rarely arises from the intrapulmonary lymph nodes. In these patients, preoperative diagnosis is difficult and invasive attempts may be required.
Assuntos
Hiperplasia do Linfonodo Gigante/diagnóstico , Hiperplasia do Linfonodo Gigante/cirurgia , Pneumopatias/diagnóstico , Pneumopatias/cirurgia , Adulto , Broncoscopia , Hiperplasia do Linfonodo Gigante/patologia , Feminino , Humanos , Pneumopatias/patologia , Toracotomia , Tomografia Computadorizada por Raios XAssuntos
Hiperpigmentação/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Pele/patologia , SíndromeRESUMO
Pancreatic dermoid cysts are a rare entity. Preoperative diagnosis is difficult. The diagnosis is generally taking intraoperative. A 20-year-old female presented with epigastric pain without nausea, vomiting, diarrhea, fever, jaundice, and weight loss of one-month duration. Ultrasonography and computed tomography showed a smooth borders, solid, hyperechoic tumor within midline abdomen, without any connection to the stomach or spleen. At surgery, the entire mass was excised off of the head and inferior part of pancreas. Histopathologic evaluation revealed the rare diagnosis of a dermoid cyst. The diagnosis is difficult preoperatively in evaluating cystic pancreatic lesions by imaging. Therefore, we want to summarize the literature on this rare entity knowledge.