Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits.

BACKGROUND: Krascheninnikovia ceratoides, a perennial halophytic semi-shrub belonging to the genus Krascheninnikovia (Amarathaceae), possesses noteworthy ecological, nutritional, and economic relevance. This species is primarily distributed across arid, semi-arid, and saline-alkaline regions of the Eurasian continent, encompassing Inner Mongolia, Xinjiang, Qinghai, Gansu, Ningxia, and Tibet. RESULTS: We reported the comprehensive chloroplast (cp) genome of K. ceratoides, characterized by a circular conformation spanning 151,968 bp with a GC content of 36.60%. The cp genome encompassed a large single copy (LSC, 84,029 bp), a small single copy (SSC, 19,043 bp), and a pair of inverted repeats (IRs) regions (24,448 bp each). This genome harbored 128 genes and encompassed 150 simple sequence repeats (SSRs). Through comparative analyses involving cp genomes from other Cyclolobeae (Amarathaceae) taxa, we observed that the K. ceratoides cp genome exhibited high conservation, with minor divergence events in protein-coding genes (PCGs) accD, matK, ndhF, ndhK, ycf1, and ycf2. Phylogenetic reconstructions delineated K. ceratoides as the sister taxon to Atriplex, Chenopodium, Dysphania, and Suaeda, thus constituting a robust clade. Intriguingly, nucleotide substitution ratios (Ka/Ks) between K. ceratoides and Dysphania species for ycf1 and ycf2 genes surpassed 1.0, indicating the presence of positive selection pressure on these loci. CONCLUSIONS: The findings of this study augment the genomic repository for the Amarathaceae family and furnish crucial molecular instruments for subsequent investigations into the ecological adaptation mechanisms of K. ceratoides within desert ecosystems.

The complete chloroplast genome and phylogenetic relationship of Apocynum pictum (Apocynaceae), a Central Asian shrub and second-class national protected species of western China.

Apocynum pictum of the dogbane family, Apocynaceae, is a perennial semi-shrub species of ecological, medicinal, and economic value. It is mainly distributed in semi-arid, saline-alkaline, and desert regions of Xinjiang, Qinghai, and Gansu of western China and adjacent regions from Kazakhstan and Mongolia. Here, we reported the complete chloroplast (cp) genome of A. pictum for the first time, and we found that it had a circular structure with an estimated length of 150,749 bp and a GC content of 38.3%. The cp genome was composed of a large single copy (LSC), a single small single copy (SSC), and two inverted repeat (IR) regions, which were 81,888 bp, 17,251 bp and 25,805 bp long, respectively. The cp genome of A. pictum encoded 134 genes and contained 66 simple sequence repeats (SSRs). A comparative analysis with other cp genomes from Apocynaceae indicated that the cp genome of A. pictum was very conserved, except for subtle differences occurring in the protein-coding genes accD, ndhF, rpl22, rpl32, rpoC2, ycf1 and ycf2. A phylogenetic reconstruction showed that A. pictum and A. venetum were sister species, forming a strongly supported clade with Trachelospermum. Interestingly, nucleotide substitution ratios (Ka/Ks) between A. pictum and A. venetum on accD and ndhF were >1.0, suggesting positive selective pressure on these genes. Our result enriches the genomic resources for the diverse dogbane family and provides critical molecular resources to develop future studies on ecological adaptation to desert habitats in Apocynum.