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1.
Int Immunopharmacol ; 128: 111469, 2024 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-38211480

RESUMO

Osteoarthritis (OA) is a prevalent joint disorder pathologically correlated to chondrocyte ferroptosis. Gamma-oryzanol (γ-Ory), as a first-line drug for autonomic disorders, aroused our interest because of its antioxidant, lipid-lowering, and hypoglycemic potential. The purpose of this study was to investigate the potential impact and mechanism of γ-Ory in treating OA. And the inhibition of γ-Ory in extracellular matrix molecule (ECM) degradation, ferroptosis, and Keap1-Nrf2 binding in IL-1ß-exposed chondrocytes was detected via immunoblotting, immunofluorescence, and co-immunoprecipitation. Micro-CT, SO staining, and immunofluorescence have been conducted to assess the impact of γ-Ory treatment on ACLT-mediated OA in rats at both imaging and histological stages. We found that γ-Ory dose-dependently suppressed IL-1ß-induced ECM deterioration and chondrocyte ferroptosis. Our animal experiments revealed that γ-Ory delayed ACLT-mediated OA development. Mechanistically, γ-Ory interfered with the binding of Keap1 to Nrf2 to promote the latter's nuclear import, thereby increasing the expression of detoxification enzymes. Summarily, our works support γ-Ory's potential as a candidate drug for the treatment of OA.


Assuntos
Ferroptose , Osteoartrite , Fenilpropionatos , Animais , Ratos , Condrócitos/metabolismo , Interleucina-1beta/metabolismo , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Osteoartrite/tratamento farmacológico , Fenilpropionatos/uso terapêutico
2.
Dalton Trans ; 50(11): 4017-4027, 2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33660718

RESUMO

Zinc-vanadium oxides have been attracting increasing consideration as anode materials for lithium/sodium-ion batteries (LIBs and SIBs) recently. Present applications are hampered by issues, including their inferior electric conductivity and enormous volume variation. Herein, nitrogen-doped carbon wrapped Zn3V3O8 (Zn3V3O8/NC) microspheres composed of abundant nanosheets were developed as an anode material by a self-assembly strategy and subsequent surface decoration. The resulting Zn3V3O8/NC porous hybrid exhibited a high specific capacity, impressive rate capability, and long-term cycling stability for both LIBs and SIBs. Notably, the superior electrochemical properties could be assigned to novel meso/microporous features, hybrid nitrogen-doped carbon, and mixed storage mechanisms.

3.
J Colloid Interface Sci ; 587: 260-270, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33360899

RESUMO

In order to cope with the problem of insufficient lithium metal reserves, sodium ion batteries (SIBs) are proposed and extensively studied for the next-generation batteries. In our work, hierarchical NiCo2Se4 nanoneedles/nanosheets are deposited on the skeleton of N-doped three dimensional porous graphene (NPG) by a convenient solvothermal method and subsequent gas-phase selenization process. Compared with NiCo2Se4 powder, the optimized NiCo2Se4/N-doped porous graphene composite (denoted as NCS@NPG) as self-supporting anode exhibits the excellent electrode activity for SIBs, with a specific capacity of 500 mAh/g and 257 mAh/g at a current density of 0.2 A/g and 6.4 A/g, respectively. The high specific capacity as well as rate capacity can be attributed to the three-dimensional graphene skeleton with high electrical conductivity and pore structure, which provides convenient ion and electron transmission channels.

4.
J Colloid Interface Sci ; 563: 318-327, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31884252

RESUMO

Molybdenum dioxides (MoO2) featuring low cost and high theoretical capacity endow them competitive anode materials for lithium-ion batteries (LIBs)/sodium-ion batteries (SIBs). However, the low electrical conductivity and severe volume expansion occurring during the ion insertion/extraction process hamper their practical application. Herein, a novel dual-annealing design has been developed for the synthesis of highly uniform MoO2 nanopopcorns decorated with nitrogen-doped carbon shell (MoO2/NC). Owing to the unique structural characteristics and vital amorphous NC component, the MoO2/NC nanopopcorn hybrid composite exhibits stabilized charge storage capacity of 1073 mAh g-1 after 200 cycles for LIBs, while 301 mAh g-1 after 500 cycles for SIBs at 0.5 A g-1. Furthermore, when the current density increases to 5 A g-1, the specific capacity could still maintain 630 mAh g-1 and 174 mAh g-1 for LIBs and SIBs, respectively, which disclose the outstanding rate capability of MoO2/NC nanopopcorn anode.

5.
Dalton Trans ; 49(31): 10928-10938, 2020 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-32720939

RESUMO

α-Phase molybdenum trioxide (α-MoO3) is one of the promising anode materials for lithium storage due to its high theoretical capacity and unique intercalation reaction mechanism. Herein, through an efficient step-by-step solvothermal synthesis strategy, multi-layered MoO3 nanosheets are encapsulated by nitrogen-doped carbon (NC) and ultrathin TiO2 double-shells to obtain hierarchical core-shell nanospheres (MoO3@TiO2@NC). The unique nanostructure enables shortening the Li+ diffusion distance, buffer the volume change during the intercalation/deintercalation process, and increase the active sites for the electrochemical reaction. Based on the hierarchical nanostructure and the synergistic effect of each component, the MoO3@TiO2@NC electrode exhibits a high Li+ storage capacity around 979.6 mA h g-1 after 200 cycles at 0.2 A g-1, a stable cycle performance of 800.3 mA h g-1 at 1 A g-1 after 700 cycles and an excellent rate capability of 418.0 mA h g-1 at 5 A g-1. Furthermore, the MoO3@TiO2@NC-based coin-type full cell with a commercial LiNi1/3Mn1/3Co1/3O2 cathode exhibited a good cycling stability at 0.2 A g-1 for 100 cycles (∼190 mA h g-1) and rate capability (134 mA h g-1 at 5 A g-1).

6.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 26(1): 49-51, 2010 Jan.
Artigo em Zh | MEDLINE | ID: mdl-20056089

RESUMO

AIM: To prepare mouse monoclonal antibodies (mAb) against AGR2 (human homolog of xenopus anterior gradient 2), to characterize these antibodies' properties, and to develop potential applications. METHODS: BALB/c mice were immunized with AGR2-MBP (maltose binding protein) fusion protein. The mAb was prepared by hybridoma technique and purified by protein G affinity chromatography. The titer and specificity of the mAb was determined by ELISA and Western blot respectively. The mAb was then further characterized by immunoprecipitation, immunofluorescent staining and tumor cell inhibition assay. RESULTS: One clone of hybridoma, 18A4, secreting specific mAb against AGR2, was obtained. The Ig subclass of the mAb was IgG1 (kappa). The titer of the mAb was 1 x 10(-6);. Western blot analysis showed specific binding of 18A4 with both recombinant and native AGR2 in cell extract. Immunofluorescent staining using 18A4 mAb demonstrated specific staining of AGR2 in the cytoplasma of Breast cancer cell line MCF7. Immunoprecipitation assay confirmed that this mAb could specifically bind and effectively precipitate the native AGR2. mAb 18A4 could also inhibit the growth of breast cancer cell MCF7. CONCLUSION: The mAb anti-AGR2 with high titer and specificity has been obtained. This mAb, 18A4, can be used in most molecular biology studies including Western blot, immunostaining, immunoprecipitation. It also inhibited the growth of cancer cells and therefore is a potential therapeutic starting point. It is a useful tool for the functional study of AGR2 and for the diagnosis and potential treatment of certain cancers.


Assuntos
Anticorpos Monoclonais/análise , Imunoglobulina G/análise , Proteínas/imunologia , Animais , Anticorpos Monoclonais/genética , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/isolamento & purificação , Especificidade de Anticorpos , Western Blotting , Linhagem Celular Tumoral , Feminino , Humanos , Imunoglobulina G/imunologia , Imunoglobulina G/isolamento & purificação , Imunoprecipitação , Camundongos , Camundongos Endogâmicos BALB C , Mucoproteínas , Proteínas Oncogênicas , Proteínas/genética
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