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3.
Yi Chuan ; 29(12): 1504-8, 2007 Dec.
Artigo em Zh | MEDLINE | ID: mdl-18065387

RESUMO

Genomic DNAs from Arctic fox were cleaved with restriction enzymes. The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis. Forty-two satellite sequences were obtained by sequencing. Sequence analyses revealed that the satellite monomer in length was 737 bp, with an average G+C content of 51.9%. There were 91%-97% homology among the monomers, and the monomer consisted of three subrepeats of approximately 245 bp in length with 51%-54% homology. The satellite DNAs were specific for canidae, and had 74% homology to the dog centromere-associated nucleotide sequences. In order to distinguish from another satellite DNAs of canidae genome, the satellite was designated as alpha satellite DNA.


Assuntos
DNA Satélite/genética , Raposas/genética , Animais , Sequência de Bases , Southern Blotting , Clonagem Molecular , Enzimas de Restrição do DNA/metabolismo , DNA Satélite/metabolismo , Feminino , Genômica , Masculino , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
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