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1.
PLoS One ; 9(6): e99839, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24936797

RESUMO

Shell beads appear to have been one of the earliest examples of personal adornments. Marine shells identified far from the shore evidence long-distance transport and imply networks of exchange and negotiation. However, worked beads lose taxonomic clues to identification, and this may be compounded by taphonomic alteration. Consequently, the significance of this key early artefact may be underestimated. We report the use of bulk amino acid composition of the stable intra-crystalline proteins preserved in shell biominerals and the application of pattern recognition methods to a large dataset (777 samples) to demonstrate that taxonomic identification can be achieved at genus level. Amino acid analyses are fast (<2 hours per sample) and micro-destructive (sample size <2 mg). Their integration with non-destructive techniques provides a valuable and affordable tool, which can be used by archaeologists and museum curators to gain insight into early exploitation of natural resources by humans. Here we combine amino acid analyses, macro- and microstructural observations (by light microscopy and scanning electron microscopy) and Raman spectroscopy to try to identify the raw material used for beads discovered at the Early Bronze Age site of Great Cornard (UK). Our results show that at least two shell taxa were used and we hypothesise that these were sourced locally.


Assuntos
Aminoácidos/química , Exoesqueleto/química , Bivalves/química , Exoesqueleto/anatomia & histologia , Animais , Organismos Aquáticos , Bivalves/anatomia & histologia , Bivalves/classificação , Fósseis , Humanos , Análise de Componente Principal , Proteínas/química , Reprodutibilidade dos Testes , Análise Espectral Raman
2.
PLoS One ; 9(4): e93854, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24718458

RESUMO

Isotopic and molecular analysis on human, fauna and pottery remains can provide valuable new insights into the diets and subsistence practices of prehistoric populations. These are crucial to elucidate the resilience of social-ecological systems to cultural and environmental change. Bulk collagen carbon and nitrogen isotopic analysis of 82 human individuals from mid to late Holocene Brazilian archaeological sites (∼6,700 to ∼1,000 cal BP) reveal an adequate protein incorporation and, on the coast, the continuation in subsistence strategies based on the exploitation of aquatic resources despite the introduction of pottery and domesticated plant foods. These results are supported by carbon isotope analysis of single amino acid extracted from bone collagen. Chemical and isotopic analysis also shows that pottery technology was used to process marine foods and therefore assimilated into the existing subsistence strategy. Our multidisciplinary results demonstrate the resilient character of the coastal economy to cultural change during the late Holocene in southern Brazil.


Assuntos
Conservação dos Recursos Naturais , Ecossistema , Aminoácidos/metabolismo , Animais , Apatitas/metabolismo , Arqueologia , Teorema de Bayes , Osso e Ossos/metabolismo , Brasil , Isótopos de Carbono , Cerâmica , Colágeno/metabolismo , Geografia , Humanos , Marcação por Isótopo , Lipídeos/isolamento & purificação , Isótopos de Nitrogênio , Fatores de Tempo
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