Cationic tantalum oxide nanoparticle contrast agent for micro computed tomography reveals articular cartilage proteoglycan distribution and collagen architecture alterations.

OBJECTIVE: Cationic tantalum oxide nanoparticles (Ta2O5-cNPs), as a newly introduced contrast agent for computed tomography of cartilage, offer quantitative evaluation of proteoglycan (PG) content and biomechanical properties. However, knowledge on the depth-wise impact of cartilage constituents on nanoparticle diffusion, particularly the influence of the collagen network, is lacking. In this study, we aim to establish the depth-dependent relationship between Ta2O5-cNP diffusion and cartilage constituents (PG content, collagen content and network architecture). METHODS: Osteochondral samples (n = 30) were harvested from healthy equine stifle joints (N = 15) and the diffusion of 2.55 nm diameter cationic Ta2O5-cNPs into the cartilage was followed with micro computed tomography (µCT) imaging for up to 96 hours. The diffusion-related parameters, Ta2O5-cNP maximum partition (Pmax) and diffusion time constant, were compared against biomechanical and depth-wise structural properties. Biomechanics were assessed using stress-relaxation and sinusoidal loading protocols, whereas PG content, collagen content and collagen network architecture were determined using digital densitometry, Fourier-transform infrared spectroscopy and polarized light microscopy, respectively. RESULTS: The Pmax correlates with the depth-wise distribution of PGs (bulk Spearman's ρ = 0.87, p < 0.001). More open collagen network architecture at the superficial zone enhances intake of Ta2O5-cNPs, but collagen content overall decreases the intake. The Pmax values correlate with the equilibrium modulus (ρ = 0.80, p < 0.001) of articular cartilage. CONCLUSION: This study establishes the feasibility of Ta2O5-cNPs for the precise and comprehensive identification of biomechanical and structural changes in articular cartilage via contrast-enhanced µCT.

Hypoxia negatively affects senescence in osteoclasts and delays osteoclastogenesis.

Cellular senescence, that is, the withdrawal from the cell cycle, combined with the acquirement of the senescence associated secretory phenotype has important roles during health and disease and is essential for tissue remodeling during embryonic development. Osteoclasts are multinucleated cells, responsible for bone resorption, and cell cycle arrest during osteoclastogenesis is well recognized. Therefore, the aim of this study was to investigate whether these cells should be considered senescent and to assess the influence of hypoxia on their potential senescence status. Osteoclastogenesis and bone resorption capacity of osteoclasts, cultured from CD14+ monocytes, were evaluated in two oxygen concentrations, normoxia (21% O2 ) and hypoxia (5% O2 ). Osteoclasts were profiled by using specific staining for proliferation and senescence markers, qPCR of a number of osteoclast and senescence-related genes and a bone resorption assay. Results show that during in vitro osteoclastogenesis, osteoclasts heterogeneously obtain a senescent phenotype. Furthermore, osteoclastogenesis was delayed at hypoxic compared to normoxic conditions, without negatively affecting the bone resorption capacity. It is concluded that osteoclasts can be considered senescent, although senescence is not uniformly present in the osteoclast population. Hypoxia negatively affects the expression of some senescence markers. Based on the direct relationship between senescence and osteoclastogenesis, it is tempting to hypothesize that contents of the so-called senescence associated secretory phenotype (SASP) not only play a functional role in matrix resorption, but also may regulate osteoclastogenesis.

EquiMoves: A Wireless Networked Inertial Measurement System for Objective Examination of Horse Gait.

In this paper, we describe and validate the EquiMoves system, which aims to support equine veterinarians in assessing lameness and gait performance in horses. The system works by capturing horse motion from up to eight synchronized wireless inertial measurement units. It can be used in various equine gait modes, and analyzes both upper-body and limb movements. The validation against an optical motion capture system is based on a Bland-Altman analysis that illustrates the agreement between the two systems. The sagittal kinematic results (protraction, retraction, and sagittal range of motion) show limits of agreement of ± 2.3 degrees and an absolute bias of 0.3 degrees in the worst case. The coronal kinematic results (adduction, abduction, and coronal range of motion) show limits of agreement of - 8.8 and 8.1 degrees, and an absolute bias of 0.4 degrees in the worst case. The worse coronal kinematic results are most likely caused by the optical system setup (depth perception difficulty and suboptimal marker placement). The upper-body symmetry results show no significant bias in the agreement between the two systems; in most cases, the agreement is within ±5 mm. On a trial-level basis, the limits of agreement for withers and sacrum are within ±2 mm, meaning that the system can properly quantify motion asymmetry. Overall, the bias for all symmetry-related results is less than 1 mm, which is important for reproducibility and further comparison to other systems.

Survey-based investigation of sports and leisure horse owners' approaches to, and expectations of, equine veterinary care.

BACKGROUND: Client satisfaction depends on the balance between expectations and service experience. Previous research identified seven aspects of equine veterinary professional conduct that are important for client satisfaction: quality of care, quality of service, horsemanship, transfer of knowledge, financial aspects, interpersonal skills and professionalism. METHODS: By employing a cross-sectional study design through a survey-based investigation, horse owners' initial contact preferences and their perceptions of the importance of various aspects of veterinary care in different scenarios were explored. Categories included professional versus amateur and competitive versus non-competitive horse owners. Quantitative data analysis was performed. RESULTS: Data from 1153 participants revealed that horse owners promptly contacted veterinarians for colic (92.7%) but delayed for lameness (51.8%) and pre-purchase examinations (63.0%). Overall, quality of care emerged as the most important aspect of veterinary care for horse owners, with financial aspects considered least important. Competitive and professional horse owners prioritised financial aspects and professionalism, whereas non-professional and non-competitive horse owners prioritised quality of care and interpersonal skills (p < 0.005). LIMITATIONS: Survey distribution relied on a snowball effect, internet access was necessary and the study exclusively represents the Western equine community. Potential bias should be acknowledged. CONCLUSION: The perceived importance of various aspects of veterinary care varies depending on the nature of the consultation and the horse owner type. Tailoring veterinary services can improve client satisfaction by aligning with diverse expectations.

Composition, architecture and biomechanical properties of articular cartilage in differently loaded areas of the equine stifle.

BACKGROUND: Strategies for articular cartilage repair need to take into account topographical differences in tissue composition and architecture to achieve durable functional outcome. These have not yet been investigated in the equine stifle. OBJECTIVES: To analyse the biochemical composition and architecture of three differently loaded areas of the equine stifle. We hypothesise that site differences correlate with the biomechanical characteristics of the cartilage. STUDY DESIGN: Ex vivo study. METHODS: Thirty osteochondral plugs per location were harvested from the lateral trochlear ridge (LTR), the distal intertrochlear groove (DITG) and the medial femoral condyle (MFC). These underwent biochemical, biomechanical and structural analysis. A linear mixed model with location as a fixed factor and horse as a random factor was applied, followed by pair-wise comparisons of estimated means with false discovery rate correction, to test for differences between locations. Correlations between biochemical and biomechanical parameters were tested using Spearman's correlation coefficient. RESULTS: Glycosaminoglycan content was different between all sites (estimated mean [95% confidence interval (CI)] for LTR 75.4 [64.5, 88.2], for intercondylar notch (ICN) 37.3 [31.9, 43.6], for MFC 93.7 [80.1109.6] µg/mg dry weight), as were equilibrium modulus (LTR2.20 [1.96, 2.46], ICN0.48 [0.37, 0.6], MFC1.36 [1.17, 1.56] MPa), dynamic modulus (LTR7.33 [6.54, 8.17], ICN4.38 [3.77, 5.03], MFC5.62 [4.93, 6.36] MPa) and viscosity (LTR7.49 [6.76, 8.26], ICN16.99 [15.88, 18.14], MFC8.7 [7.91,9.5]°). The two weightbearing areas (LTR and MCF) and the non-weightbearing area (ICN) differed in collagen content (LTR 139 [127, 152], ICN176[162, 191], MFC 127[115, 139] µg/mg dry weight), parallelism index and angle of collagen fibres. The strongest correlations were between proteoglycan content and equilibrium modulus (r: 0.642; p: 0.001), dynamic modulus (r: 0.554; p < 0.001) and phase shift (r: -0.675; p < 0.001), and between collagen orientation angle and equilibrium modulus (r: -0.612; p < 0.001), dynamic modulus (r: -0.424; p < 0.001) and phase shift (r: 0.609; p < 0.001). MAIN LIMITATIONS: Only a single sample per location was analysed. CONCLUSIONS: There were significant differences in cartilage biochemical composition, biomechanics and architecture between the three differently loaded sites. The biochemical and structural composition correlated with the mechanical characteristics. These differences need to be acknowledged by designing cartilage repair strategies.

INTRODUCTION/CONTEXTE: Les stratégies de réparation du cartilage articulaire doivent tenir compte des différences topographiques en ce qui a trait à la composition et l'architecture des tissues, afin d'obtenir un résultat durable et fonctionnel. Celles­ci n'ont pas encore été étudiées chez le grasset équin. OBJECTIFS: Analyser la composition biochimique et l'architecture de trois régions du grasset portant une quantité de poids différente. Nous émettons l'hypothèse que les différences entre régions seront corrélées aux caractéristiques biomécaniques du cartilage. TYPE D'ÉTUDE: Étude ex vivo. MÉTHODES: Trente échantillons ostéochondraux par site ont été récoltés à partir de la lèvre latérale de la trochlée fémorale (LTR), le sillon intertrochléaire distal (DITG) et le condyle fémoral médial (MFC). Ceux­ci ont été soumis à des tests biochimiques, biomécaniques et une analyse structurelle. Un modèle linéaire mixte avec localisation comme facteur fixe et cheval comme facteur randomisé a été appliqué. Puis, ont suivi des comparaisons par paires de moyennes estimées avec contrôle du taux de fausses découvertes, pour tester les différences entre les divers sites. Les corrélations entre les paramètres biochimiques et biomécaniques ont été testé par le coefficient de corrélation Spearman. RÉSULTATS: Le contenu en glycosaminoglycans était différent à chacun des sites (moyenne estimée [95% CI] pour LTR 75.4 [64.5, 88.2], pour ICN 37.3 [31.9, 43.6], pour MFC 93.7[80.1109.6]µg/mg matière sèche), tout comme le module d'équilibre (LTR2.20 [1.96, 2.46], ICN0.48 [0.37, 0.6], MFC1.36 [1.17, 1.56] MPa), le module dynamique (LTR7.33 [6.54, 8.17], ICN4.38[3.77, 5.03], MFC5.62[4.93, 6.36] MPa) et la viscosité (LTR7.49[6.76, 8.26], ICN16.99 [15.88, 18.14], MFC8.7 [7.91, 9.5]°). Les deux régions portant du poids (LTR et MFC) et la région ne supportant pas de poids (ICN) diffèrent par rapport à leur contenu en collagène (LTR 139 [127152], ICN176 [162191], MFC 127 [115139] µg/mg matière sèche), à l'index de parallélisle et à l'angle des fibres de collagène. Les corrélations les plus fortes étaient entre le contenu en protéoglycans et le module d'équilibre (r: 0.642; p: 0.001), le module dynamique (r: 0.554; p < 0.001) et le changement de phase (r:−0.675; p < 0.001), et entre l'angle d'orientation du collagène et le module d'équilibre (r:−0.612; p < 0.001), le module dynamique (r:−0.424; p < 0.001) et le changement de phase (r: 0.609;p:<0.001). LIMITES PRINCIPALES: Seulement un échantillon par site a été soumis aux analyses. CONCLUSIONS: Il existe des différences significatives dans la composition biochimique, biomécanique et l'architecture du cartilage entre les trois sites échantillonnés. La composition biochimique et structurelle corrèle avec les caractéristiques mécaniques. Ces différences doivent être prises en compte lors de la création de stratégies de réparation du cartilage.

Osteochondral repair: evaluation with sweep imaging with fourier transform in an equine model.

PURPOSE: To evaluate the status of articular cartilage and bone in an equine model of spontaneous repair by using the sweep imaging with Fourier transform (SWIFT) magnetic resonance (MR) imaging technique. MATERIALS AND METHODS: Experiments were approved by the Utrecht University Animal Ethics Committee. Six-millimeter-diameter chondral (n = 5) and osteochondral (n = 5, 3-4 mm deep into subchondral bone) defects were created in the intercarpal joints of seven 2-year-old horses and examined with SWIFT at 9.4 T after spontaneous healing for 12 months. Conventional T2 maps and gradient-echo images were obtained for comparison, and histologic assessment of cartilage and micro-computed tomography (CT) of bone were performed for reference. Signal-to-noise ratio (SNR) analysis was performed, and a radiologist evaluated the MR images. Structural bone parameters were derived from SWIFT and micro-CT datasets. Significance of differences was investigated with the Wilcoxon signed rank test and Pearson correlation analysis. RESULTS: SWIFT was able to depict the different outcomes of spontaneous healing of focal chondral versus osteochondral defects. SWIFT produced constant signal intensity throughout cartilage, whereas T2 mapping showed elevated T2 values (P = .06) in repair tissue (mean T2 in superficial region of interest in an osteochondral lesion = 50.0 msec ± 10.2) in comparison to adjacent intact cartilage (mean T2 = 32.7 msec ± 4.2). The relative SNR in the subchondral plate with SWIFT (0.91) was more than four times higher than that with conventional fast spin-echo (0.12) and gradient-echo (0.19) MR imaging. The correlation between bone volume-to-tissue volume fractions determined with SWIFT and micro-CT was significant (r = 0.83, P < .01). CONCLUSION: SWIFT enabled assessment of spontaneous osteochondral repair in an equine model.

A prospective study on a cohort of horses and ponies selected for participation in the European Eventing Championship: reasons for withdrawal and predictive value of fitness tests.

BACKGROUND: Eventing is generally recognized as a challenging equestrian discipline and wastage figures for this discipline are relatively high. There is a need for information that provides insight into the causes of wastage and withdrawal from competition, for animal welfare and economic reasons. The aim of the present investigation was to conduct a prospective study following the entire national selection of event horses (n = 20) and ponies (n = 9) in the Netherlands that prepared for the European Championship in 2010 (ponies) and 2011 (horses), noting causes of withdrawal and monitoring fitness using standardized exercise tests (SETs), with heart rate (HR; beats/min), speed (V; m/s) and plasma lactate concentrations (LA; mmol/L) as measured parameters. RESULTS: In SET-I, performed at the beginning of the season, horses (n = 17) had a mean VLA4 (V at LA 4 mmol/L) of 10.3 ± 0.4 m/s with a mean V200 (V at 200 beats/min) of 11.4 ± 0.8 m/s and ponies (n = 9) a mean VLA4 of 7.8 ± 0.9 m/s and V200 of 9.6 ± 0.7 m/s. Before SET-II, performed six weeks before the European Championship, 16/20 horses and 6/9 ponies were withdrawn. The most common reason for withdrawal was locomotor injury (9/16 horses, 4/6 ponies; P < 0.001 and P = 0.011, respectively). Other reasons included an animal 'not meeting the competition criteria' (4/16 horses, 2/6 ponies) and being sold (3/16 horses). Animals were divided on the basis of VLA4 and recovery-HR during SET-I into good and average performers. Average performers were significantly more likely to be injured (50.0%) than good performers (0%, P = 0.05). In a subpopulation of ten horses, in which all condition training sessions were evaluated for HR and speed, HRpeak was significantly lower in horses that stayed sound (186 ± 9 beats/min) compared with horses withdrawn from training and competition because of injury (201 ± 5 beats/min; P = 0.016). CONCLUSIONS: Of the national selection, 45% of all animals were unavailable for the European Championship because of locomotor injuries. Field tests were useful in assessing the potential injury risk, as individuals with better fitness indices (good performers) were less likely to become injured than average performers. Furthermore, monitoring of training sessions showed predictive value for future injuries, as horses withdrawn because of injury later on showed already higher peak HRs during condition training than horses that stayed sound. Therefore the increase in peak HR seemed to precede visible lameness in a horse.

Engage and enjoy-investigating predictors of employee engagement and work satisfaction in equine veterinary professionals.

Introduction: Individuals working in the field of veterinary care are regularly affected by their profession. High levels of responsibility to often provide life-saving health care to animals combined with having to manage owners' expectations and irregular working hours can cause considerable levels of work-related stress among professionals in equine veterinary practice. On the positive side, research also shows that working in the veterinary profession can have a positive impact on personal wellbeing and feelings of fulfillment. A limited number of studies has investigated work satisfaction and engagement among veterinarians across the globe, and none specifically in the equine veterinary work field. The aim of the current study was to identify relevant predictors of employee engagement and work satisfaction in relation to demographic and work environment related factors in the equine veterinary profession. Methods: A cross-sectional study design was used to investigate work satisfaction and employee engagement among equine veterinary professionals from the UK, the US and the Netherlands using an online survey. Results: Results suggest that levels of work engagement and satisfaction in the veterinary profession may be gauged using four factors. These factors encompass Pride and purpose (the extent to which personal core values align with the mission of the employer, i.e., the veterinary practice), Company culture and relationship with management (the manner in which staff members interact with each other and the management), Working conditions and compensation (formal employment conditions relating to responsibilities and rewards and levels of collegiality) and Team culture and learning possibilities (encouragement to pursue personal and professional growth). Discussion: Findings underline the importance of being particularly mindful of inexperienced colleagues, those with demanding family commitments and, where feasible, of providing employees with a modicum of autonomy in order to ensure a satisfied equine veterinary workforce.

Movement asymmetries in horses presented for prepurchase or lameness examination.

BACKGROUND: The increasing popularity of objective gait analysis makes application in prepurchase examinations (PPE) a logical next step. Therefore, there is a need to have more understanding of asymmetry during a PPE in horses described on clinical evaluation as subtly lame. OBJECTIVES: The objective of this study is to objectively compare asymmetry in horses raising minor vet concerns in a PPE and in horses raising major vet concerns with that found in horses presented with subtle single-limb lameness, and to investigate the effect of age/discipline on the clinicians' interpretation of asymmetry on the classification of minor vet concerns in a PPE. STUDY DESIGN: Clinical case-series. METHODS: Horses presented for PPE (n = 98) or subjectively evaluated as single limb low-grade (1-2/5) lame (n = 24, 13 forelimb lame, 11 hindlimb lame), from the patient population of a single clinic, were enrolled in the study provided that owners were willing to participate. Horses undergoing PPE were assigned a classification of having minor vet concerns (n = 84) or major vet concerns (n = 14) based on findings during the dynamic-orthopaedic part of the PPE. Lame horses were only included if pain-related lameness was confirmed by an objective improvement after diagnostic analgesia exceeding daily variation determined for equine symmetry parameters using optical motion capture. Clinical evaluation was performed by six different clinicians, each with ≥8 years of equine orthopaedic experience. Vertical movement symmetry was measured using optical motion capture, simultaneously with the orthopaedic examination. Data were analysed using previously described parameters and mixed model analysis and least squares means were used to calculate differences between groups. RESULTS: There was no effect of age or discipline on the levels of asymmetry within PPE horses raising minor vet concerns. MinDiff and RUD of the head discriminated between forelimb lame and PPE horses raising minor vet concerns; MinDiff, MaxDiff, RUD of the Pelvis, HHDswing and HHDstance did so for hindlimb lameness. Two lameness patterns differentiated both forelimb and hindlimb lame from PPE horses with minor vet concerns: RUD Poll + MinDiff Withers - RUD Pelvis and RUD Pelvis + RUD Poll - MinDiff Withers. Correcting for vertical range of motion enabled differentiation of PPE horses with minor vet concerns from PPE horses with major vet concerns. MAIN LIMITATIONS: Objective data only based on trot on soft surface, limited number of PPE horses with major vet concerns. CONCLUSIONS: Combinations of kinematic parameters discriminate between PPE horses with minor vet concerns and subtly lame horses, though overlap exists.

Visual lameness assessment in comparison to quantitative gait analysis data in horses.

BACKGROUND: Quantitative gait analysis offers objective information to support clinical decision-making during lameness workups including advantages in terms of documentation, communication, education, and avoidance of expectation bias. Nevertheless, hardly any data exist comparing outcome of subjective scoring with the output of objective gait analysis systems. OBJECTIVES: To investigate between- and within-veterinarian agreement on primary lame limb and lameness grade, and to determine relationships between subjective lameness grade and quantitative data, focusing on differences between (1) veterinarians, (2) live vs video assessment, (3) baseline assessment vs assessment following diagnostic analgesia. STUDY DESIGN: Clinical observational study. METHODS: Kinematic data were compared to subjective lameness assessment by clinicians with ≥8 years of orthopaedic experience. Subjective assessments and kinematic data for baseline trot-ups and response to 48 diagnostic analgesia interventions in 23 cases were included. Between and within-veterinarian agreement was investigated using Cohen's Kappa (κ). Asymmetry parameters for kinematic data ('forelimb lame pattern', 'hindlimb lame pattern', 'overall symmetry', 'vector sum head', 'pelvic sum') were determined, and used as outcome variables in mixed models; explanatory variables were subjective lameness grade and its interaction with (1) veterinarian, (2) live or video evaluation and (3) baseline or diagnostic analgesia assessment. RESULTS: Agreement on lame limb between live and video assessment was 'good' between and within veterinarians (median κ = 0.64 and κ = 0.53). There was a positive correlation between subjective scoring and measured asymmetry. The relationship between lameness grade and objective asymmetry differed slightly between (1) veterinarians (for all combined parameters, p-values between P < .001 and 0.04), (2) between live and video assessments ('forelimb lame pattern', 'overall symmetry', both P ≤ .001), and (3) between baseline and diagnostic analgesia assessment (all combined parameters, between P < .001 and .007). MAIN LIMITATIONS: Limited number of veterinarians (n = 4) and cases (n = 23), only straight-line soft surface data, different number of subjective assessments live vs from video. CONCLUSIONS: Overall, between- and within-veterinarian agreement on lame limb was 'good', whereas agreement on lameness grade was 'acceptable' to 'poor'. Quantitative data and subjective assessments correlated well, with minor though significant differences in the number of millimetres, equivalent to one lameness grade between veterinarians, and between assessment conditions. Differences between baseline assessment vs assessment following diagnostic analgesia suggest that addition of objective data can be beneficial to reduce expectation bias. The small differences between live and video assessments support the use of high-quality videos for documentation, communication, and education, thus, complementing objective gait analysis data.

Histological tissue healing following high-power laser treatment in a model of suspensory ligament branch injury.

BACKGROUND: High-power laser therapy gained popularity recently as a regenerative treatment for tendinitis and desmitis in the horse. However, studies evaluating the effects of laser therapy on tissue repair at the histological level in large mammals are lacking. OBJECTIVES: To evaluate the effects of high-power laser therapy on suspensory desmitis healing, using a model of suspensory ligament branch injury. STUDY DESIGN: In vivo experiments. METHODS: Standardised lesions were surgically induced in all four lateral suspensory branches of 12 healthy Warmblood horses. Laser therapy (class 4, 15W) was applied daily on two of four induced lesions for four consecutive weeks. Horses were randomly assigned to either short-term study (horses were sacrificed after 4 weeks) or long-term study (6 months). Suspensory ligament samples were scored after staining with haematoxylin-eosin and immunostaining for collagen 1- collagen 3- and factor VIII. RESULTS: In the short-term study, significantly better (lower) scores for variation in density (17% above cut-off score in treated lesions vs. 31% above cut-off score in controls, P = .03), shape of nuclei (54% vs 92%, P = .02), fibre alignment (32% vs 75%, P = .003) and fibre structure (38% vs 71%, P = .02) were found in laser-treated lesions when compared to controls. Collagen 3 expression was significantly higher (32% vs 19%, P = .006) in control lesions. In both short- and long-term studies combined, parameters lesion size (44% vs 56%, P = .02) and shape of nuclei (53% vs 84%, P = .05) scored significantly better in treated lesions. Long-term, significantly better (lower) scores were found in the laser-treated group for lesion size (15% vs 45%, P = .008) and a higher percentage above cut-off score for density of the nuclei (27% vs 9%, P = .02), compared to controls. MAIN LIMITATIONS: The model of suspensory branch injury is not an exact representation of clinical overstrain lesions. CONCLUSIONS: These results suggest that high-power laser therapy enables better lesion healing than conservative treatment.

Client satisfaction in equine veterinary practice: A structured review and qualitative synthesis.

BACKGROUND: The pressure on equine veterinarians to provide services that meet with a client's demands, that is, realizing client satisfaction, is considerable. AIM: The aim of this paper is to analyze existing literature, with a view to distill the most relevant components for client satisfaction in equine veterinary practice. MATERIALS AND METHODS: A structured literature search was conducted. Included papers were systematically organised and analysed using an inductive approach. RESULTS: Seven components relevant to client satisfaction in equine veterinary practice were identified: quality of care, quality of service, horsemanship of the veterinarian, costs of service, interpersonal skills, professional attitude and transfer of knowledge. DISCUSSION: The limited amount and variable quality of available records regarding client satisfaction in equine veterinary practice are the main limitations of this study. CONCLUSION: The seven categories identified are likely to play a fundamental role in achieving client satisfaction in equine veterinary practice. Additional research is required to validate these categories and relate them to different types of clients as well as map their needs and expectations, so that they can be used to assist equine veterinary professionals in tailoring customer experience to the individual client.

Comparisons of historical Dutch commons inform about the long-term dynamics of social-ecological systems.

Human societies and natural ecosystems are under threat by growing populations, overexploitation of natural resources and climate change. This calls for more sustainable utilization of resources based on past experiences and insights from many different disciplines. Interdisciplinary approaches to studies of historical commons have potential to identify drivers of change and keys to success in the past, and offer advice about the management and use of shared resources in contemporary and future systems. We address these issues by applying an ecological perspective to historical data on social-ecological systems. We perform comparisons and time series analyses for nine successful Dutch commons for which high-resolution data on the regulatory activities and use of shared resources is available for on average 380 years (range 236 to 568) during the period 1300 to 1972. Within commons, institutional developments were oscillating, with periods of intense regulatory activity being separated by periods of low activity, and with the dynamics of regulations being largely independent across commons. Ecological theory posits that species that occupy similar niches should show correlated responses to environmental challenges; however, commons using more similar resources did not have more parallel or similar institutional developments. One notable exception was that sanctioning was more frequent in commons that directed more regulatory activities towards non-renewable subsoil resources, whereas there was no association between sanctioning and the use of renewable resources. This might indicate that commoners were aware of potential resource depletion and attempted to influence freeriding by actively trying to solve the underlying social dilemmas. Sanctioning regulations were more frequent during the first than during the second part of a common's life, indicating that while sanctioning might have been important for the establishment of commons it was not key to the long-term persistence of historical commons.

Asymmetries of horses walking and trotting on treadmill with and without rider.

BACKGROUND: Left-right movement symmetry is a highly desirable characteristic in sport horses. OBJECTIVES: This study compared movement symmetry in well-trained dressage horses in unridden and unrestrained position and ridden in a dressage frame, and investigated possible associations between gaits. STUDY DESIGN: Experimental study. METHODS: Seven sound, high-level dressage horses were measured at walk and sitting trot on a treadmill at several speeds under two conditions: with and without rider. Left-right differences in stance duration, stance protraction and retraction based on longitudinal hoof positions, ipsilateral limb tracking, minimum and maximum vertical positions of the dorsal spinous processes of the sixth thoracic (T6), third sacral vertebrae (S3) and wing of atlas, and vertical ground reaction forces were calculated and analysed in mixed models. RESULTS: At walk, five body variables indicated increased asymmetry in the ridden condition compared with unridden condition: forelimb stance duration (unridden/ridden left-right differences 9 vs 13 ms; P = .008), forelimb stance protraction (P = .004), stance retraction (P = .001) and first force peak (P = .003), and hindlimb stance retraction (P = .01). At trot, six body variables were more asymmetrical in the ridden condition: forelimb stance duration (2.5 vs 3.8 ms, P = .004); hindlimb stance protraction (P < .0001) and retraction (P = .01), T6 minimum (4 vs 6 mm, P = .001), T6 maximum (9 vs 11 mm, P = .01) and S3 maximum (6 vs 12 mm, P < .001). Five variables had significant associations between asymmetries at walk and trot, but only three demonstrated a positive slope. MAIN LIMITATIONS: A limited number of horses and riders were studied. Measurements were performed on a treadmill. CONCLUSIONS: High-level horses moved slightly more asymmetrically when ridden in a dressage frame than in the unridden condition.

Eco-evolutionary perspectives on emergence, dispersion and dissolution of historical Dutch commons.

Historical commons represent self-governed governance regimes that regulate the use and management of natural and man-made shared resources. Despite growing scientific interests, analyses of commons evolution and temporal dynamics are rare and drivers of change (birth, adaptation, dissolution) remain obscure. We apply an interdisciplinary approach and address these issues from an eco-evolutionary perspective. Analyses of > 400 Dutch commons over more than a millennium (between the 9th and the 20th century) uncovered that most commons originated between 1200 and 1700, and that there was a particularly high rate of evolution during 1300-1550, a pattern intermediate to gradualism and punctuated equilibrium in biological evolution. Dissolutions of commons were rare prior to 1800 and peaked around 1850, comparable to a mass extinction in biology. Temporal trends in number, spatial distribution, density, and dispersion of historical commons were distinctive and resembled developments seen at the levels of species and individuals in the growth of biological communities and populations, in that they showed signs of saturation determined by the abundance and distribution of resources. The spatiotemporal dynamics of commons also pointed to important roles of social, economic and political factors, such as new reclamations of resources and pressure on resources due to population growth. Despite internal and external pressures, the self-governing commons studied here were very successful, in the sense that they persisted for on average >350 years. There was a weak positive relationship between the use of multiple resources and the lifespan of commons, resembling associations between diversity and persistence seen in biological systems. It is argued that eco-evolutionary perspectives can further the understanding of the long-term dynamics of commons as institutions for collective action, vitalize future research, improve management of shared goods, and advise about sustainable utilization of finite resources.

T2* mapping in an equine articular groove model: Visualizing changes in collagen orientation.

T2* mapping is promising for the evaluation of articular cartilage collagen. In this work, a groove model in a large animal is used as a model for posttraumatic arthritis. We hypothesized that T2* mapping could be employed to differentiate between healthy and (subtly) damaged cartilage. Eight carpal joints were obtained from four adult Shetland ponies that had been included in the groove study. In this model, grooves were surgically created on the proximal articular surface of the intermediate carpal bone (radiocarpal joint) and the radial facet of the third carpal bone (middle carpal joint) by either coarse disruption or sharp incision. After 9 months, T2* mapping of the entire carpal joint was carried out on a 7.0-T whole-body magnetic resonance imaging (MRI) scanner by means of a gradient echo multi-echo sequence. Afterwards, assessment of collagen orientation was carried out based on Picrosirius Red-stained histological sections, visualized by polarized light microscopy (PLM). The average T2* relaxation time in grooved samples was lower than in contralateral control sites. Opposite to the grooved areas, the "kissing sites" had a higher average T2* relaxation time than the grooved sites. PLM showed mild changes in orientation of the collagen fibers, particularly around blunt grooves. This work shows that T2* relaxation times are different in healthy cartilage vs (early) damaged cartilage, as induced by the equine groove model. Additionally, the average T2* relaxation times are different in kissing lesions vs the grooved sites.

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking.

Bioactive hydrogels based on naturally-derived polymers are of great interest for regenerative medicine applications. Among naturally-derived polymers, silk fibroin has been extensively explored as a biomaterial for tissue engineering due to its unique mechanical properties. Here, we demonstrate the rapid gelation of cell-laden silk fibroin hydrogels by visible light-induced crosslinking using riboflavin as a photo-initiator, in presence of an electron acceptor. The gelation kinetics were monitored by in situ photo-rheometry. Gelation was achieved in minutes and could be tuned owing to its direct proportionality to the electron acceptor concentration. The concentration of the electron acceptor did not affect the elastic modulus of the hydrogels, which could be altered by varying the polymer content. Further, the biocompatible riboflavin photo-initiator combined with sodium persulfate allowed for the encapsulation of cells within silk fibroin hydrogels. To confirm the cytocompatibility of the silk fibroin formulations, three cell types (articular cartilage-derived progenitor cells, mesenchymal stem cells and dental-pulp-derived stem cells) were encapsulated within the hydrogels, which associated with a viability >80% for all cell types. These results demonstrated that fast gelation of silk fibroin can be achieved by combining it with riboflavin and electron acceptors, which results in a hydrogel that can be used in tissue engineering and cell delivery applications.

Tough magnesium phosphate-based 3D-printed implants induce bone regeneration in an equine defect model.

One of the important challenges in bone tissue engineering is the development of biodegradable bone substitutes with appropriate mechanical and biological properties for the treatment of larger defects and those with complex shapes. Recently, magnesium phosphate (MgP) doped with biologically active ions like strontium (Sr2+) have shown to significantly enhance bone formation when compared with the standard calcium phosphate-based ceramics. However, such materials can hardly be shaped into large and complex geometries and more importantly lack the adequate mechanical properties for the treatment of load-bearing bone defects. In this study, we have fabricated bone implants through extrusion assisted three-dimensional (3D) printing of MgP ceramics modified with Sr2+ ions (MgPSr) and a medical-grade polycaprolactone (PCL) polymer phase. MgPSr with 30 wt% PCL (MgPSr-PCL30) allowed the printability of relevant size structures (>780 mm3) at room temperature with an interconnected macroporosity of approximately 40%. The printing resulted in implants with a compressive strength of 4.3 MPa, which were able to support up to 50 cycles of loading without plastic deformation. Notably, MgPSr-PCL30 scaffolds were able to promote in vitro bone formation in medium without the supplementation with osteo-inducing components. In addition, long-term in vivo performance of the 3D printed scaffolds was investigated in an equine tuber coxae model over 6 months. The micro-CT and histological analysis showed that implantation of MgPSr-PCL30 induced bone regeneration, while no bone formation was observed in the empty defects. Overall, the novel polymer-modified MgP ceramic material and extrusion-based 3D printing process presented here greatly improved the shape ability and load-bearing properties of MgP-based ceramics with simultaneous induction of new bone formation.

High-Power Laser Therapy Improves Healing of the Equine Suspensory Branch in a Standardized Lesion Model.

High-power laser therapy is often used as a treatment for human sport injuries but controlled standardized studies on its efficacy are lacking. The technique has also been introduced in the equine field and recently promising results were reported in a retrospective study focusing on 150 sporthorses suffering from tendinopathy and desmopathy of the SDFT, DDFT, suspensory ligament, and suspensory branches. The goal of the present study was to evaluate the effect of high-power laser in a standardized lesion model in horses. Lesions were created in all lateral suspensory branches of 12 warmblood horses. In each horse, 2 of the 4 lesioned branches were treated daily with a multi-frequency high-power laser for 4 weeks. Color Doppler ultrasonography was performed during and after the treatment period. Six horses were euthanized 4 weeks post-surgery (short-term) and 6 were further rehabilitated until 6 months and then euthanized (long-term). High-field MRI evaluation was performed on all cadaver limbs. On ultrasound, transverse size of the lesion was significantly smaller after 2- and 3 months (p = 0.026 and p = 0.015) in the treated branches. The expected post-surgery enlargement of the lesion circumference and cross-sectional area (CSA) over time, was significantly lower in the short-term laser treated group (p = 0.016 and p = 0.010). Treated lesions showed a significantly increased Doppler signal during treatment (p < 0.001) compared with control. On MRI, in the short and long-term group, the CSA of the lesions was significantly smaller (p = 0.002), and the mean signal significantly lower in the treatment groups (p = 0.006). This standardized controlled study shows that multi-frequency high-power laser therapy significantly improves healing of a suspensory branch ligament lesion.

Orthotopic Bone Regeneration within 3D Printed Bioceramic Scaffolds with Region-Dependent Porosity Gradients in an Equine Model.

The clinical translation of three-dimensionally printed bioceramic scaffolds with tailored architectures holds great promise toward the regeneration of bone to heal critical-size defects. Herein, the long-term in vivo performance of printed hydrogel-ceramic composites made of methacrylated-oligocaprolactone-poloxamer and low-temperature self-setting calcium-phosphates is assessed in a large animal model. Scaffolds printed with different internal architectures, displaying either a designed porosity gradient or a constant pore distribution, are implanted in equine tuber coxae critical size defects. Bone ingrowth is challenged and facilitated only from one direction via encasing the bioceramic in a polycaprolactone shell. After 7 months, total new bone volume and scaffold degradation are significantly greater in structures with constant porosity. Interestingly, gradient scaffolds show lower extent of remodeling and regeneration even in areas having the same porosity as the constant scaffolds. Low regeneration in distal regions from the interface with native bone impairs ossification in proximal regions of the construct, suggesting that anisotropic architectures modulate the cross-talk between distant cells within critical-size defects. The study provides key information on how engineered architectural patterns impact osteoregeneration in vivo, and also indicates the equine tuber coxae as promising orthotopic model for studying materials stimulating bone formation.