Indoor/outdoor relationships, signatures, sources, and carcinogenic risk assessment of polycyclic aromatic hydrocarbons-enriched PM2.5 in an emerging port of northern China.

Humans spend most of their time in indoor environments, thus a thorough understanding of indoor and outdoor PM2.5-bound polycyclic aromatic hydrocarbons (PAHs) origins for accurate assessment of health risks is required. In the present study, 84 pairs of PM2.5 samples from indoor (laboratory) and outdoor (campus) locations were collected from April to December 2018 in Caofeidian, China. The annual median concentration of PM2.5 outdoors was 90.80 µg/m3, 9.08 times higher than the annual standard of WHO guideline (10 µg/m3). Indoor PM2.5 annual median concentration (41.80 µg/m3) was also higher than the annual standard of ASHRAE guideline (15 µg/m3). The annual median concentrations of ∑18PAHs indoors (44.23 ng/m3) and outdoors (189.6 ng/m3) were highest in winter and descended in the order of autumn > spring > summer. Contrary to summer and autumn, indoor/outdoor concentration ratios were less than 1 in spring and winter, indicating that the contribution of outdoor particle infiltration was more significant than that of indoor sources. The positive matrix factorization model suggested that indoor PAHs came from three sources: vehicle emissions (43%), biomass burning (37%), industry emissions, and coal combustion (20%). Outdoor PAHs came from four sources: petroleum volatilization (39%), vehicle emissions (30%), coal combustion (18%), and biomass burning (13%). The incremental lifetime cancer risk values of indoor and outdoor PAHs in winter exceeded the acceptable level (10-6), and the carcinogenic risk of adults was higher than that of children and teenagers. These results indicated that simultaneous monitoring of indoor and outdoor PAHs is recommended for accurate assessment of health risk, and the analysis in the current work should be helpful to formulate policies to reduce PAHs emissions.

Harbin: a quantitation PCR analysis tool.

OBJECTIVES: To enable analysis and comparisons of different relative quantitation experiments, a web-browser application called Harbin was created that uses a quantile-based scoring system for the comparison of samples at different time points and between experiments. RESULTS: Harbin uses the standard curve method for relative quantitation to calculate concentration ratios (CRs). To evaluate if different datasets can be combined the Harbin quantile bootstrap test is proposed. This test is more sensitive in detecting distributional differences between data sets than the Kolmogorov-Smirnov test. The utility of the test is demonstrated in a comparison of three grapevine leafroll associated virus 3 (GLRaV-3) RT-qPCR data sets. CONCLUSIONS: The quantile-based scoring system of CRs will enable the monitoring of virus titre or gene expression over different time points and be useful in other genomic applications where the combining of data sets are required.

Transfer of 129I to freshwater fish species within Fukushima and Chernobyl exclusion zones.

Unique data is reported on the transfer of 129I iodine from freshwaters to fish as well as the internal distribution within fish from the Fukushima and Chernobyl exclusion zones (ChEZ). Samples of water, sediments and fish were collected in the contaminated ponds Inkyozaka and Suzuuchi, and in the less contaminated Abukuma river in Fukushima, as well as in the contaminated Glubokoye lake and in the less contaminated Starukha lake in ChEZ. In water, 129I was mainly present as low molecular mass (LMM) and negatively charged species, while a minor fraction was associated with colloidal fraction, most probably organic material in water. The sediment-water 129I apparent distribution coefficients, Kd, ranged from 225 to 329 L/kg, equal that of stable iodine, but did not correlate with 129I/127I ratio or 129I/137Cs ratio as the environmental distribution of radioactive iodine was different from that of stable iodine and radioactive cesium. Concentration ratios (CR) of 129I in muscle of freshwater fish ranged from 85 to 544 across waterbodies with limited water exchange, similar in Fukushima and Chernobyl, but varied with respect to fish species. Thus, this is the first results on the transfer of 129I to freshwater fish, showing that the CR for freshwater fish is higher than CR reported for marine fish. Concentrations of 129I in fish muscle were, however, lower than in the intestinal content, indicating the influence of more contaminated dietary ingredients probably of terrestrial origin based due to δ13C signal on as well as of biodilution. The present results highlighted also that the radiation dose in fish was highly inhomogeneously distributed. Based on the present 129I/127I atomic ratio of 10-5 in the most contaminated fish in the ponds in Fukushima and Glubokoye lake in Chernobyl, however, a radiation dose of 10 µSv/y would not pose any harm to the fish population.

Allometric-kinetic model predictions of concentration ratios for anthropogenic radionuclides across animal classes and food selection.

Protection of the environment from radiation fundamentally relies on dose assessments for non-human biota. Many of these dose assessments use measured or predicted concentrations of radionuclides in soil or water combined with Concentration Ratios (CRs) to estimate whole body concentrations in animals and plants, yet there is a paucity of CR data relative to the vast number of potential taxa and radioactive contaminants in the environment and their taxon-specific ecosystems. Because there are many taxa each having very different behaviors and biology, and there are many possible bioavailable radionuclides, CRs have the potential to vary by orders-of-magnitude, as often seen in published data. Given the diversity of taxa, the International Commission on Radiological Protection (ICRP) has selected 12 non-human biota as reference animals and plants (RAPs), while the U.S. Department of Energy (DOE) uses the non-taxon specific categories of terrestrial, riparian, and aquatic animals. The question we examine here, in part, is: are these RAPs and categorizations sufficient to adequately protect all species given the broad diversity of animals in a region? To explore this question, we utilize an Allometric-Kinetic (A-K) model to calculate radionuclide-specific CRs for common animal classes, which are then further subcategorized into herbivores, omnivores, carnivores, and invertebrate detritivores. Comparisons in CRs among animal classes exhibited only small differences, but there was order of magnitude differences between herbivores, carnivores, and especially detritivores, for many radionuclides of interest. These findings suggest that the ICRP RAPs and the DOE categories are reasonable, but their accuracy could be improved by including sub-categories related to animal dietary ecology and biology. Finally, comparisons of A-K model predicted CR values to published CRs show order-of-magnitude variations, providing justification for additional studies of animal assimilation across radionuclides, environmental conditions, and animal classes.

How dynamic transfer models can complement an equilibrium-based approach: Case studies of radiocesium transfer to forest trees following accidental atmospheric release.

Accidental release of radionuclides caused by nuclear accidents like those in Fukushima and Chernobyl can result in pulses of radioactivity entering the forest environment. Due to intense recycling in the forest, equilibrium between radioactivity concentrations in trees and in soil may not be reached during the period of short-term radionuclide transport following the accident. Another question arises as to whether the equilibrium hypothesis using empirical concentration ratios (CRs) can be applied to the long-term period. Using two atmospheric 137Cs fallout scenarios in the Fukushima and Chernobyl sites, this study investigated whether the CR approach could provide conservative predictions of 137Cs levels in trees following 137Cs fallout events by comparing predictions from the CR approach using data gathered for trees by the IAEA to those from dynamic transfer models and actual measured data. The inter-comparisons also aimed to investigate whether the CR approach could account for the variability of 137Cs levels across different tree organs. The results showed that caution may be necessary when using the CR approach, which relies on the IAEA dataset, to estimate 137Cs accumulation in forest trees in the short - and long term following atmospheric 137Cs fallout events. A calculation by TRIPS 2.0 demonstrated the importance of considering the distribution within tree organs for in-depth analysis of radiological impact of forest trees. Our findings suggest that it may be preferable to use CR values based on site-specific data rather than generic data collected from various sites. This is particularly relevant when studying the sites where the bioavailability of 137Cs for trees and thus possible exposures are higher. This study also showed that dynamic modeling approaches could offer an alternative means of estimating CR values of the entire tree or specific tree organs in situations where empirically derived values are not available.

Molecular signatures of organic particulates as tracers of emission sources.

Chemical signature of airborne particulates and deposition dusts is subject of study since decades. Usually, three complementary composition markers are investigated, namely, (i) specific organic compounds; (ii) concentration ratios between congeners, and (iii) percent distributions of homologs. Due to its intrinsic limits (e.g., variability depending on decomposition and gas/particle equilibrium), the identification of pollution sources based on molecular signatures results overall restricted to qualitative purposes. Nevertheless, chemical fingerprints allow drawing preliminary information, suitable for successfully approaching multivariate analysis and valuing the relative importance of sources. Here, the state-of-the-art is presented about the molecular fingerprints of non-polar aliphatic, polyaromatic (PAHs, nitro-PAHs), and polar (fatty acids, organic halides, polysaccharides) compounds in emissions. Special concern was addressed to alkenes and alkanes with carbon numbers ranging from 12 to 23 and ≥ 24, which displayed distinct relative abundances in petrol-derived spills and exhausts, emissions from microorganisms, high vegetation, and sediments. Long-chain alkanes associated with tobacco smoke were characterized by a peculiar iso/anteiso/normal homolog fingerprint and by n-hentriacontane percentages higher than elsewhere. Several concentration ratios of PAHs were identified as diagnostic of the type of emission, and the sources of uncertainty were elucidated. Despite extensive investigations conducted so far, the origin of uncommon molecular fingerprints, e.g., alkane/alkene relationships in deposition dusts and airborne particles, remains quite unclear. Polar organics resulted scarcely investigated for pollution apportioning purposes, though they looked as indicative of the nature of sources. Finally, the role of humans and living organisms as actual emitters of chemicals seems to need concern in the future.

Comparison of serum and whole blood concentrations in quetiapine overdose cases.

This study aimed to compare whole blood and serum concentrations of quetiapine in acute poisoning cases. Authentic whole blood and respective serum samples were routinely collected from patients diagnosed with blood poisoning at our University Hospital. Accordingly, whole blood and serum paired samples from nine patients (one male and eight female patients) were analyzed for quetiapine using liquid chromatography-mass spectrometry (LC-MS). Quetiapine concentrations in whole blood and serum samples ranged widely from 5.4 to 2780 ng/mL and 9.9 to 2500 ng/mL, respectively. The whole blood/serum concentration ratio was 0.5-1.1 and increased together with an increase in whole blood and serum quetiapine concentrations. The ratio was reversed at around 2500 ng/mL to > 1. Our findings suggest that whole blood concentrations are more useful than serum concentrations in diagnosing quetiapine poisonings.

Detection of amfepramone and its metabolite cathinone in human hair: Application to a uthentic cases of amfepramone use.

In recent years, overseas anti-obesity drugs including amfepramone have flowed into China through the internet or personal import by travelers. Amfepramone is controlled in China and is not available as a pharmaceutical product. It is obtainable either through the internet or imported by individuals across the border. The abuse of amfepramone is causing serious health problems. A method for the detection and quantification of amfepramone and its metabolite cathinone in human hair was developed and fully validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Approximately 10 mg of hair was weighed and pulverized with extraction solvent (a mixture of methanol: acetonitrile: 2 mM ammonium formate [pH 5.3] [25:29:46, v/v/v]). The limit of detection (LOD) and the limit of quantitation (LOQ) were 5 and 10 pg/mg, respectively. The method was linear over a concentration range from 10 to 10,000 pg/mg. The accuracy varied from -9.3% to 2.3%, with acceptable intra- and inter-day precision. The validated method was successfully applied to 17 authentic cases. The amfepramone concentrations ranged from 11.7 to 209 pg/mg, with a median of 30.2 pg/mg, and the hair cathinone concentrations ranged from 11.9 to 507 pg/mg, with a median of 54.0 pg/mg. This is the first report of amfepramone concentrations in human hair from amfepramone users. Cathinone can be incorporated into hair after amfepramone use.

Radium in New Zealand agricultural soils: Crop uptake and estimation of current and future ionising radiation dose.

Crop uptake of 226Ra over a range of key New Zealand agricultural and horticultural growing areas was analysed to establish the dietary implications of an increase in soil 226Ra activity concentrations. Thirty crop samples, covering both feed and food commodities, were quantified for 226Ra activity concentrations, and concentration ratio (CRs) from the soil activity were calculated. The calculated CRs correlated with international default values for estimating crop uptake. Variation in CRs established that there was no increase in the crop activity concentration, relative to soil 226Ra from pasture foliage at a fertiliser impacted site, with a gradient of soil 226Ra activity concentrations. Based on the calculated CRs, the upper bound of the theoretical range of dietary exposures to 226Ra was 78.1 µSv/yr for teenage boys. Future forecasting of the increased dietary dose of 226Ra that might occur at the current soil loading rate, based on current fertiliser activity concentrations, confirmed that long-term loading of soil with 226Ra is unlikely to present a dietary risk. The forecast model calculated that the increase in dietary ionising radiation burden is unlikely to reach thresholds requiring regulatory intervention for two millennia.

Combined toxicity of nano-TiO2 and Cd2+ to Scenedesmus obliquus: Effects at different concentration ratios.

The continuous release of manufactured nanomaterials (MNMs) to environments raised concerns on their combined toxicological risks with co-existing contaminants, since MNMs might severely alter the environmental behavior and fate of the contaminants. In this study, the combined toxicity of nano-sized titanium dioxide (nTiO2) and cadmium (Cd2+) to the green alga Scenedesmus obliquus and the underlying physicochemical mechanisms were investigated for the first time at different concentration ratios of Cd2+ to nTiO2 to closely mimic the realistic environment scenarios where the concentration ratios of nTiO2 to other contaminants are constantly changing. Our results suggested that under the co-exposure to different concentration ratios of Cd2+ to nTiO2, the co-exposure contaminants exhibited three different combined toxicity modes (antagonistic, partially additive, and synergistic). Specifically, antagonistic combined toxicity was observed under co-exposure to a low concentration ratio of nTiO2 to Cd2+ as the absorption by nTiO2 decreased the bioavailability of Cd2+. However, the partially additive and synergistic combined toxicity occurred when the proportion of nTiO2 in the co-exposure system was relatively high, which would mechanically and/or oxidatively damage the alga cell structures. Even worse, as a carrier of Cd2+, nTiO2 enhanced the amount of Cd2+ entering cells, which significantly enhanced the toxicity of Cd2+ to algae. Overall, we demonstrated that concentration ratios of nTiO2 to Cd2+ play an important role in determining the combined toxicity mode, which would provide a novel reference to environmental and health risk assessment of co-exposure to conventional pollutants and MNMs.

210Po levels and distribution in different environmental compartments from a coastal lagoon. The case of Briozzo lagoon, Uruguay.

This paper presents the levels and distribution of 210Po in different compartments of a coastal lagoon on the east coast of Uruguay (South America). Activity concentrations of 210Po have been obtained in different matrices, such as water, superficial sediments, clams (Diplodon sp.), freshwaters snails (Pomacea sp.), zooplankton, and fishes (Jenynsia sp.), collected at different points of the lagoon and during several sampling campaigns. In addition, the organic matter content of the sediment was determined to study the variation of 210Po along the lagoon. The activity concentrations of 210Po in the water samples are in the range between 1.1 ± 0.2-3.5 ± 0.4 mBq/L while in the sediment samples vary between 17.1 ± 1.4 and 540 ± 12 Bq/kg, DW. In the case of biota, the ranges obtained were 182 ± 5-265 ± 6 Bq/kg, DW in clams and 134 ± 4-1245 ± 16 Bq/kg, DW in snail samples. A good correlation of 210Po with the organic fractions of the sediment was observed (r = 0.8798, p-value < 0.001), being obtained high values for the distribution coefficient Kd (104 -105). In the biota samples, a clear difference was observed in the 210Po concentration values in both species, mainly due to the different feeding habits of both aquatic organisms, as it is reflected in the associated concentration ratios (CR). In this paper, a good set of results of 210Po, Kd, and CR have been obtained in different matrices, enhancing the limited archives available for modelers concerning these parameters for 210Po and freshwater systems.

On-site investigation of the concentration and size distribution characteristics of airborne fungi in a university library.

It is important to investigate fungal air quality in libraries because they represent a complex indoor environment. The aim of the study was to quantitatively investigate airborne fungal contamination levels based on field measurements in autumn and winter in four selected library rooms (compact stack, lending room, reading room, study room) in a university library building, as well as the effects of several factors on the culturability of airborne fungi. Airborne fungal levels varied by room, with the highest fungal levels in the reading room (634 ± 275 CFU/m3) and the lowest in the lending room (486 ± 177 CFU/m3). Airborne fungal concentrations were significantly different with seasonal variation (p < 0.05) for all rooms except for the reading room. The size distribution analysis showed that the most airborne fungi were 1.1-3.2 µm in size; based on the schematic diagram of the human respiratory system, more than 80% of airborne fungi could be deposited in the lower respiratory tract (0.65-4.7 µm). Indoor/outdoor airborne fungal concentration ratios were below 1.0 for all four rooms during autumn and winter, showing that outdoor fungi are the main source of indoor fungi. Pearson correlations showed that the fungal concentration was significantly positively correlated with both temperature (r = 0.531, p < 0.05) and relative humidity (r = 0.555, p < 0.05). Indoor temperature, indoor relative humidity and number of open windows significantly positively affected airborne fungal concentration in a multiple linear regression model (p < 0.05). This paper provides fundamental data on fungal contamination that can help experts in indoor air quality to develop guidelines for airborne fungi in libraries and create a safe environment for library patrons and staff.

137Cs and 40K concentration ratios (CRs) in annual and perennial plants in the Caspian coast.

This research attempted to investigate the plant/soil concentration ratios (CRs) of 137Cs and 40K in plants that grow on the Anzali Lagoon of the Caspian coast, Iran. The activity concentrations of 137Cs and 40K were measured in soil samples, annual plants (Echinochloa crus-galli, Digitaria sanguinalis, and Trifolium repens), and perennial plants (Phragmites australis, and Cynodon dactylon). The relationship of activity concentration with soil particle density, and pH of soil samples, CR, and 137Cs/40K discrimination factor (DF) were determined. The activity concentration of 137Cs in the soil, annual plants, and perennial plants ranged between 12- and 124 (Bq/kg dry weight), 2- and 17 (Bq/kg dw), and 2 - and 14 (Bq/kg dw), respectively. The geometric mean (GM) values of DF in annual and perennial plants were 0.57 and 0.55, respectively. The CR for 137Cs and 40K varied as a non-linear relation in annual plants and perennial plants. This study showed that the CRs for 137Cs in annual plants were higher than the CRs for perennial plants.

Nonhuman biota dose rate estimation from liquid effluent releases during normal operations of light water reactors using the LADTAP II computer code.

The LADTAP II computer code was used to estimate the dose rates to seven nonhuman biota (fish, algae, invertebrate, muskrat, raccoon, heron, and duck) from liquid effluent releases during the normal operations of new light water reactor facilities in the United States. The estimated dose rates to nonhuman biota depended on the nuclear power plant design but were orders of magnitude less than the 'derived consideration reference levels' (DCRL) in International Commission on Radiological Protection (ICRP) Publication 108. The estimated dose rates were at least three orders of magnitude lower than the guideline values identified by the International Atomic Energy Agency (IAEA) and the National Council on Radiation Protection and Measurements to cause observable changes in populations of biota. Radionuclides that contributed most of the dose rates for seven nonhuman biota depended on the nuclear power plant design. The differences in the estimated dose rates to seven nonhuman biota calculated from the default bioaccumulation factors used in the LADTAP II computer code and the dose rates calculated from the concentration ratios of radionuclides in the IAEA Technical Report Series (TRS) wildlife handbook were less than one order of magnitude for most default biota in LADTAP. The concentration ratios for some radionuclides in liquid effluent releases from nuclear power plant designs that contributed more than 5% of the internal dose rates for invertebrates (P, and Ru) and algae (La and Pr) were not available in the IAEA TRS wildlife handbook.

Transfer parameters for ICRP's Reference Animals and Plants in a terrestrial Mediterranean ecosystem.

A system for the radiological protection of the environment (or wildlife) based on Reference Animals and Plants (RAPs) has been suggested by the International Commission on Radiological Protection (ICRP). To assess whole-body activity concentrations for RAPs and the resultant internal dose rates, transfer parameters are required. However, transfer values specifically for the taxonomic families defined for the RAPs are often sparse and furthermore can be extremely site dependent. There is also a considerable geographical bias within available transfer data, with few data for Mediterranean ecosystems. In the present work, stable element concentrations (I, Li, Be, B, Na, Mg, Al, P, S, K. Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Mo, Ag, Cd, Cs, Ba, Tl, Pb and U) in terrestrial RAPs, and the corresponding whole-body concentration ratios, CRwo, were determined in two different Mediterranean ecosystems: a Pinewood and a Dehesa (grassland with disperse tree cover). The RAPs considered in the Pinewood ecosystem were Pine Tree and Wild Grass; whereas in the Dehesa ecosystem those considered were Deer, Rat, Earthworm, Bee, Frog, Duck and Wild Grass. The CRwo values estimated from these data are compared to those reported in international compilations and databases.

137Cs and 90Sr IN lizards of Semipalatinsk test site.

The paper provides research results of 137Cs and 90Sr radionuclides concentrations in bodies of Lacertidae family lizards, inhabiting different parts of Semipalatinsk Test Site, and the parameters of these radionuclides' transfer into lizards' bodies. It shows that high activity concentration of radionuclides in lizards' bodies can be noticed if they live directly at locally contaminated areas. Since the distance from contaminated spots exceeds home range of the studied animals, no increased values of radionuclides' activity were found in the animal bodies. At some individual radioactively contaminated spots, very high activity concentrations of 90Sr radionuclide up to 7.8 × 105 Bq kg-1 were found in lizards. So under certain conditions, lizards can significantly contribute to radionuclides redistribution in the natural environment. Mean concentration ratios (CR) of radionuclides were as follows: 137Cs-6.2 × 10-3, 90Sr-1.1 × 10-2.

Whole-organism concentration ratios in wildlife inhabiting Australian uranium mining environments.

Wildlife concentration ratios for 226Ra, 210Pb, 210Po and isotopes of Th and U from soil, water, and sediments were evaluated for a range of Australian uranium mining environments. Whole-organism concentration ratios (CRwo-media) were developed for 271 radionuclide-organism pairs within the terrestrial and freshwater wildlife groups. Australian wildlife often has distinct physiological attributes, such as the lower metabolic rates of macropod marsupials as compared with placental mammals. In addition, the Australian CRswo-media originate from tropical and semi-arid climates, rather than from the temperate-dominated climates of Europe and North America from which most (>90%) of internationally available CRwo-media values originate. When compared, the Australian and non-Australian CRs are significantly different for some wildlife categories (e.g. grasses, mammals) but not others (e.g. shrubs). Where differences exist, the Australian values were higher, suggesting that site-, or region-specific CRswo-media should be used in detailed Australian assessments. However, in screening studies, use of the international mean values in the Wildlife Transfer Database (WTD) appears to be appropriate, as long as the values used encompass the Australian 95th percentile values. Gaps in the Australian datasets include a lack of marine parameters, and no CR data are available for freshwater phytoplankton, zooplankton, insects, insect larvae or amphibians; for terrestrial environments, there are no data for amphibians, annelids, ferns, fungi or lichens & bryophytes. The new Australian specific parameters will aide in evaluating remediation plans and ongoing operations at mining and waste sites within Australia. They have also substantially bolstered the body of U- and Th-series CRwo-media data for use internationally.

Fine particulate-bound polycyclic aromatic hydrocarbons in vehicles in Rome, Italy.

Urban commuters are exposed to elevated levels of air pollutants, especially in heavily polluted areas and traffic congested roads. In order to assess the contribution of commuting to citizens' exposure, measurements of fine particulate (PM2.5) and polycyclic aromatic hydrocarbons (PAHs) were carried out in cars, busses, and metro trains, within the LIFE+ EXPAH Project. Monitoring campaigns were performed in Rome, Italy, from April 2011 to August 2012. Inside the busses, the concentration of total PAHs ranged from 2.7 to 6.6 ng/m3 during the winter and from 0.34 to 1.51 ng/m3 in the summer. In cars, internal concentrations were in the range 2.2-7.3 and 0.46-0.82 ng/m3, respectively, in the 2-year time. Analogous differences between seasons were observed examining the benzo[a]pyrene-equivalent carcinogenicity. In the metro trains, total PAHs ranged from 1.19 to 2.35 ng/m3 and PM2.5 ranged from 17 to 31 µg/m3. The PM2.5 concentration in all transport modes ranged from 10 to 160 µg/m3 during the cold season and 15-48 µg/m3 during the warm time. The average inside-to-outside ratio (R I/O) was found to exceed 1.0 for PM2.5 only in busses, probably due to dust re-suspension caused by crowding and passenger activity. The molecular PAH signature suggests that vehicle emissions and biomass combustion were the major sources of commuters' exposure to these toxicants in Rome. According to linear regression analysis, the PAH concentrations inside the vehicles were linked to those detected outside. Statistically significant differences (p < 0.05) were found between the in-vehicle locations and the urban pollution network stations, with higher PAH values detected, on the average, in these latter.

Development and evaluation of a regression-based model to predict cesium-137 concentration ratios for saltwater fish.

Data from published studies and World Wide Web sources were combined to develop a regression model to predict (137)Cs concentration ratios for saltwater fish. Predictions were developed from 1) numeric trophic levels computed primarily from random resampling of known food items and 2) K concentrations in the saltwater for 65 samplings from 41 different species from both the Atlantic and Pacific Oceans. A number of different models were initially developed and evaluated for accuracy which was assessed as the ratios of independently measured concentration ratios to those predicted by the model. In contrast to freshwater systems, were K concentrations are highly variable and are an important factor in affecting fish concentration ratios, the less variable K concentrations in saltwater were relatively unimportant in affecting concentration ratios. As a result, the simplest model, which used only trophic level as a predictor, had comparable accuracies to more complex models that also included K concentrations. A test of model accuracy involving comparisons of 56 published concentration ratios from 51 species of marine fish to those predicted by the model indicated that 52 of the predicted concentration ratios were within a factor of 2 of the observed concentration ratios.

Bioaccumulation of the artificial Cs-137 and the natural radionuclides Th-234, Ra-226, and K-40 in the fruit bodies of Basidiomycetes in Greece.

The bioaccumulation of artificial Cs-137 and natural radionuclides Th-234, Ra-226, and K-40 by Basidiomycetes of several species is studied and evaluated in relation to their substratum soils. For this reason, 32 fungal samples, representing 30 species of Basidiomycetes, were collected along with their substratum soil samples, from six selected sampling areas in Greece. The fungal fruit bodies and the soil samples were properly treated and the activity concentrations of the studied radionuclides were measured by gamma spectroscopy. The measured radioactivity levels ranged as follows: Cs-137 from <0.1 to 87.2 ± 0.4 Bq kg(-1) fresh weight (F.W.), Th-234 from <0.5 ± 0.9 to 28.3 ± 25.5 Bq kg(-1) F.W., Ra-226 from <0.3 to 1.0 ± 0.5 Bq kg(-1) F.W., and K-40 from 56.4 ± 3.0 to 759.0 ± 28.3 Bq kg(-1) F.W. The analysis of the results supported that the bioaccumulation of the studied natural radionuclides and Cs-137 is dependent on the species and the functional group of the fungi. Fungi were found to accumulate Th-234 and not U-238. What is more, potential bioindicators for each radionuclide among the 32 species studied could be suggested for each habitat, based on their estimated concentration ratios (CRs). The calculation of the CRs' mean values for each radionuclide revealed a rank in decreasing order for all the species studied.