Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

Can digital health democratize health care?

Much has been said about the potential of digital health technologies for democratizing health care. But how exactly is democratization with digital health technologies conceptualized and what does it involve? We investigate debates on the democratization of health care with digital health and identify that democratization is being envisioned as a matter of access to health information, health care, and patient empowerment. However, taking a closer look at the growing pool of empirical data on digital health, we argue that these technologies come short of materializing these goals, given the unequal health outcomes they facilitate. Building on this evidence, we argue that not only debates on democratization need to be connected to concerns of social determinants of health but also debates on the impact of digital health need to go far beyond democratization and engage with concerns of health justice.

Disinformation and Regime Survival.

Disinformation has transformed into a global issue and while it is seen as a growing concern to democracy today, autocrats have long used it as a part of their propaganda repertoire. Yet, no study has tested the effect of disinformation on regime stability and breakdown beyond country-specific studies. Drawing on novel measures from the Digital Society Project (DSP) estimating the levels of disinformation disseminated by governments across 148 countries between 2000-2022 and from the Episodes of Regime Transformation (ERT) dataset, we provide the first global comparative study of disinformation and survival of democratic and authoritarian regimes, respectively. The results show that in authoritarian regimes, disinformation helps rulers to stay in power as regimes with higher levels of disinformation are less likely to experience democratization episodes. In democracies, on the other hand, disinformation increases the probability of autocratization onsets. As such, this study is the first to provide comparative evidence on the negative effects of disinformation on democracy as well as on the prospects of democratization.

Democratizing Artificial Intelligence Imaging Analysis With Automated Machine Learning: Tutorial.

Deep learning-based clinical imaging analysis underlies diagnostic artificial intelligence (AI) models, which can match or even exceed the performance of clinical experts, having the potential to revolutionize clinical practice. A wide variety of automated machine learning (autoML) platforms lower the technical barrier to entry to deep learning, extending AI capabilities to clinicians with limited technical expertise, and even autonomous foundation models such as multimodal large language models. Here, we provide a technical overview of autoML with descriptions of how autoML may be applied in education, research, and clinical practice. Each stage of the process of conducting an autoML project is outlined, with an emphasis on ethical and technical best practices. Specifically, data acquisition, data partitioning, model training, model validation, analysis, and model deployment are considered. The strengths and limitations of available code-free, code-minimal, and code-intensive autoML platforms are considered. AutoML has great potential to democratize AI in medicine, improving AI literacy by enabling "hands-on" education. AutoML may serve as a useful adjunct in research by facilitating rapid testing and benchmarking before significant computational resources are committed. AutoML may also be applied in clinical contexts, provided regulatory requirements are met. The abstraction by autoML of arduous aspects of AI engineering promotes prioritization of data set curation, supporting the transition from conventional model-driven approaches to data-centric development. To fulfill its potential, clinicians must be educated on how to apply these technologies ethically, rigorously, and effectively; this tutorial represents a comprehensive summary of relevant considerations.

[Participatory approaches in age(ing) research : Definitions, fields of application and challenges in different research stages]. / Partizipative Alter(n)sforschung : Definitionen, Anwendungsfelder und Herausforderungen in unterschiedlichen Forschungsphasen.

There is much to be gained from participatory research: it can increase the closeness of research to everyday life, the acceptance of the resulting practical implications and holds the potential to fundamentally democratize scientific knowledge production. It is not surprising that this is not without irritation on the part of academic researchers and their institutional environment as well as on the part of nonacademically trained co-researchers. Based on an inspection of the relevant literature this article outlines the different understanding and definitions of participatory age(ing) research, its current fields of application, and utilization in different phases of the research process. Subsequently, the challenges that participatory approaches in age(ing) research can pose in these different fields and phases are discussed and possible solutions are outlined.

The Wrong Place at the Wrong Time? Territorial Autonomy and Conflict During Regime Transitions.

This article evaluates how territorial autonomy affects ethnic mobilization and conflict during regime transitions. Previous research has highlighted its conflict-inducing role during prominent transition contexts. Alternatively, it has shown its pacifying role in the "average" case, without distinguishing transition periods from stable contexts. Addressing these gaps, we argue that the de-escalatory consequences of autonomy depend on critical stabilizing factors which are themselves "muted" during transitions. We test our expectations in a cross-national analysis, covering all regime transitions between 1946 and 2017. We also revisit the 1989 transition from Communism, focusing on the role of "inherited" autonomy in the post-communist successor states. This enables us to address concerns whereby autonomy is offered to ward off transitions or whereby transitions are themselves induced by mobilization. Our findings indicate that during transitions, territorial autonomy increases the likelihood of ethnic mobilization, government concessions in response, and violent escalation where these are not forthcoming.

An Evolutionary Institutionalist Explanation of the Philippines' Militaristic COVID-19 Approach.

The Philippines undertook a "militaristic" approach to COVID-19. This has been attributed, directly or indirectly, to the strong political will of their current president, Rodrigo Duterte. Going against this narrative, this work presents an alternative framing of the issue. Drawing on concepts from institutionalist theory, this work argues that such a militaristic policy should be viewed as a continuation of the long-standing presence of militaristic ideas in civilian affairs. The dominance of such ideas has been normalized, not recently via Duterte but decades ago, owing to the failure to have a genuine liberal democracy after the fall of the Marcos regime. Overall, this work presents a historical grounding, using a new evolutionary framework, as to why institutions or policies - in this case the militaristic COVID-19 approach - come to fruition.

Migration and Democratization in Brazil: The Case of Electoral Participation and Competition.

Deepening democratization in Brazil has coincided with sustained flows of domestic migration, which raises an important question of whether migration deepens or depresses democratic development in migrant-sending regions. Whereas earlier perspectives have viewed migration as a political "brain drain," we contend that out-migration can generate resources that promote democratic processes back home. We investigate the role of migration in two aspects of democratization: electoral participation and competition. The analyses are based on spatial panel data models of mayoral election results across all municipalities between 1996 and 2012. The results show that migration increases electoral participation and competition in migrant-sending localities in Brazil. This study also identifies the sociopolitical context that conditions the impact of migration: the effect is most often present in the context of rural-urban migration and is more pronounced in sending localities with less democratic political structures. Moreover, using spatial network models, we find evidence for the transmission of political remittances from migration destination municipalities to origin municipalities. The present study extends the research on the migration-development nexus to the political arena, thus demonstrating the value of integrating demographic processes into explanations of political change.

"Pints or half-pints": Gender, functional democratization, and the consumption of drink in Ireland.

This paper examines the relationship between the gender power balance, changes in the consumption of alcohol and changing social interdependences. The empirical setting is Ireland circa 1900 up to the present. Drawing from the works of Norbert Elias, I explain how a lessening of the power inequality between men and women was more moderate and limited up to the 1960s. The effect of this was that emancipatory changes around drinking were mainly confined to women from specific social cohorts. As the reduction in gender power inequality accelerated post 1960 it initially increased tensions between the genders, reflected in new power struggles over the social spaces in which drinking occurred and in the type of glass one should drink from. Despite the emergence of less unequal power relations, men continued to have a model setting function in relation to alcohol consumption. A central contention of the paper is the need to give greater consideration to the nature of social interdependences for they can generate a lessening of power inequalities for some social cohorts while failing to generate such a dynamic for other similar social groups.

The Politics of Aging under a Hybrid Regime: The Case of Democratization in Hong Kong.

Students of the politics of aging have mainly focused their research on the following: (1) elders' voting patterns and other forms of political participation, (2) the power and influence of older people as perceived, most notably, by politicians, and (3) the effectiveness of pressure/interest groups and lobbyists for older people. The context of their research has been large and growing national fiscal deficits and the projected growth in health care, long-term care, and age pension costs arising from population aging and the consequent calls for welfare state reform. However, an aspect of the relationship between aging and politics in which there has been little investment is that of the engagement of elders with issues and that do not bear narrowly on their own well-being or self-interest. This paper focuses upon the politics of aging in relation to the highly controversial issue of greater democratization in Hong Kong SAR, China. It seeks to provide some insight into the role played by Hong Kong's elders in shaping politics and policy under a quasi-democratic regime in order to move beyond, on the one hand, the focus by students of the politics of aging on liberal democracies and, on the other, their hitherto exclusive focus on the politics of aging in relation to old-age welfare state reform.

Fragmented Politics in Hong Kong-Analyzing the Pro-democracy Cohesion in the Legislative Council Before 2019.

This study is to analyze how the fragmentation of the pro-democracy camp affected their council voting and policy stances before 2019. The quantitative measurements including the rice and unity indices are adopted to evaluate the cohesions of the pro-Beijing and pro-democracy camps in bill voting, in which the strategies employed by the pro-democracy camp are further analyzed. Before the 2010s, the moderate democrats deliberately separated from the administration and some of them also kept distance from the radical groups. However, since the radical ideologies gained supports from time to time, the moderate democrats had been forced to follow more pro-active lines against the administration. Although the political sphere of Hong Kong has drastically changed after the 2019 Anti-extradition Protests, the cohesion of the pro-democracy parties in the previous terms of the Legislative Council still facilitates to understand the legislative process in the city. In this article, 18 then members of the Legislative Council from different parties were interviewed in 2018, providing various insights on the analysis of pro-democracy cohesion and fragmented politics in Hong Kong.

From sharecropping to equal shares: transforming the sharing economy in northeastern Brazil.

Concepts of sharing and commons are normatively and historically ambivalent. Some forms of sharing, such as sharecropping or alms-giving, proceed from and sustain asymmetrical relations to the means of life. Access to commons in other social contexts merely serves to make unequal forms of life more bearable. In other words, some expressions of sharing and commons are "functional" within hierarchical societies. Departing from these observations, this contribution traces contests over the logic of sharing, and apportioned shares of common land, from Brazil's slave period through contemporary land rights movements in the northeastern state of Bahia. For former slaves and their descendants, "freedom" often meant sharecropping on the same plantations from which they had been released. However, rural Brazilians have also succeeded in transforming shared land into more equal and equitable distributions, from "peasant breaches" that emerged in slave gardens from the early colonial period through the abolition of slavery, to land occupations that occurred in the late twentieth century. By sharing land and other material resources-especially tree seeds, seedlings, and cuttings-rural laborers have established unexpected reconfigurations in distributions of property and social recognition that exceed institutionalized norms of sharing common land. With such outcomes in view, this contribution distinguishes socially replicative and transformative sharing.

Applying Artificial Intelligence to Address the Knowledge Gaps in Cancer Care.

BACKGROUND: Rapid advances in science challenge the timely adoption of evidence-based care in community settings. To bridge the gap between what is possible and what is practiced, we researched approaches to developing an artificial intelligence (AI) application that can provide real-time patient-specific decision support. MATERIALS AND METHODS: The Oncology Expert Advisor (OEA) was designed to simulate peer-to-peer consultation with three core functions: patient history summarization, treatment options recommendation, and management advisory. Machine-learning algorithms were trained to construct a dynamic summary of patients cancer history and to suggest approved therapy or investigative trial options. All patient data used were retrospectively accrued. Ground truth was established for approximately 1,000 unique patients. The full Medline database of more than 23 million published abstracts was used as the literature corpus. RESULTS: OEA's accuracies of searching disparate sources within electronic medical records to extract complex clinical concepts from unstructured text documents varied, with F1 scores of 90%-96% for non-time-dependent concepts (e.g., diagnosis) and F1 scores of 63%-65% for time-dependent concepts (e.g., therapy history timeline). Based on constructed patient profiles, OEA suggests approved therapy options linked to supporting evidence (99.9% recall; 88% precision), and screens for eligible clinical trials on ClinicalTrials.gov (97.9% recall; 96.9% precision). CONCLUSION: Our results demonstrated technical feasibility of an AI-powered application to construct longitudinal patient profiles in context and to suggest evidence-based treatment and trial options. Our experience highlighted the necessity of collaboration across clinical and AI domains, and the requirement of clinical expertise throughout the process, from design to training to testing. IMPLICATIONS FOR PRACTICE: Artificial intelligence (AI)-powered digital advisors such as the Oncology Expert Advisor have the potential to augment the capacity and update the knowledge base of practicing oncologists. By constructing dynamic patient profiles from disparate data sources and organizing and vetting vast literature for relevance to a specific patient, such AI applications could empower oncologists to consider all therapy options based on the latest scientific evidence for their patients, and help them spend less time on information "hunting and gathering" and more time with the patients. However, realization of this will require not only AI technology maturation but also active participation and leadership by clincial experts.

The Political Economy of Clientelism: A Comparative Study of Indonesia's Patronage Democracy.

What kind of economic development curtails clientelistic politics? Most of the literature addressing this relationship focuses narrowly on vote buying, resulting in theories that emphasize the importance of declining poverty rates and a growing middle class. This article employs a combination of ethnographic fieldwork and an expert survey to engage in a first-ever, more comprehensive comparative study of within-country variation of clientelistic politics. I find a pattern that poorly matches these dominant theories: Clientelism is perceived to be less intense in rural, poverty-prone Java, while scores are high in relatively wealthy yet state-dependent provincial capitals. On the basis of these findings, I develop an alternative perspective on the relationship between economic development and clientelism. Emphasizing the importance of societal constraints, I argue that the concentration of control over economic activities fosters clientelism because it stifles the public sphere and inhibits effective scrutiny and disciplining of politico-business elites.

Untangling the proximate causes and underlying drivers of deforestation and forest degradation in Myanmar.

Political transitions often trigger substantial environmental changes. In particular, deforestation can result from the complex interplay among the components of a system-actors, institutions, and existing policies-adapting to new opportunities. A dynamic conceptual map of system components is particularly useful for systems in which multiple actors, each with different worldviews and motivations, may be simultaneously trying to alter different facets of the system, unaware of the impacts on other components. In Myanmar, a global biodiversity hotspot with the largest forest area in mainland Southeast Asia, ongoing political and economic reforms are likely to change the dynamics of deforestation drivers. A fundamental conceptual map of these dynamics is therefore a prerequisite for interventions to reduce deforestation. We used a system-dynamics approach and causal-network analysis to determine the proximate causes and underlying drivers of forest loss and degradation in Myanmar from 1995 to 2016 and to articulate the linkages among them. Proximate causes included infrastructure development, timber extraction, and agricultural expansion. These were stimulated primarily by formal agricultural, logging, mining, and hydropower concessions and economic investment and social issues relating to civil war and land tenure. Reform of land laws, the link between natural resource extraction and civil war, and the allocation of agricultural concessions will influence the extent of future forest loss and degradation in Myanmar. The causal-network analysis identified priority areas for policy interventions, for example, creating a public registry of land-concession holders to deter corruption in concession allocation. We recommend application of this analytical approach to other countries, particularly those undergoing political transition, to inform policy interventions to reduce forest loss and degradation.

Screening Out Controversy: Human Genetics, Emerging Techniques of Diagnosis, and the Origins of the Social Issues Committee of the American Society of Human Genetics, 1964-1973.

In the years following World War II, and increasingly during the 1960s and 1970s, professional scientific societies developed internal sub-committees to address the social implications of their scientific expertise (Moore, Disrupting Science: Social Movements, American Scientists, and the Politics of the Military, 1945-1975. Princeton: Princeton University Press, 2008). This article explores the early years of one such committee, the American Society of Human Genetics' "Social Issues Committee," founded in 1967. Although the committee's name might suggest it was founded to increase the ASHG's public and policy engagement, exploration of the committee's early years reveals a more complicated reality. Affronted by legislators' recent unwillingness to seek the expert advice of human geneticists before adopting widespread neonatal screening programs for phenylketonuria (PKU), and feeling pressed to establish their relevance in an increasingly resource-scarce funding environment, committee members sought to increase the discipline's expert authority. Painfully aware of controversy over abortion rights and haunted by the taint of the discipline's eugenic past, however, the committee proceeded with great caution. Seeking to harness interest in and assert professional control over emerging techniques of genetic diagnosis, the committee strove to protect the society's image by relegating ethical and policy questions about their use to the individual consciences of member scientists. It was not until 1973, after the committee's modest success in organizing support for a retrospective public health study of PKU screening and following the legalization of abortion on demand, that the committee decided to take a more publicly engaged stance.

Optical network democratization.

The current Internet infrastructure is not able to support independent evolution and innovation at physical and network layer functionalities, protocols and services, while at same time supporting the increasing bandwidth demands of evolving and heterogeneous applications. This paper addresses this problem by proposing a completely democratized optical network infrastructure. It introduces the novel concepts of the optical white box and bare metal optical switch as key technology enablers for democratizing optical networks. These are programmable optical switches whose hardware is loosely connected internally and is completely separated from their control software. To alleviate their complexity, a multi-dimensional abstraction mechanism using software-defined network technology is proposed. It creates a universal model of the proposed switches without exposing their technological details. It also enables a conventional network programmer to develop network applications for control of the optical network without specific technical knowledge of the physical layer. Furthermore, a novel optical network virtualization mechanism is proposed, enabling the composition and operation of multiple coexisting and application-specific virtual optical networks sharing the same physical infrastructure. Finally, the optical white box and the abstraction mechanism are experimentally evaluated, while the virtualization mechanism is evaluated with simulation.

European do-it-yourself (DIY) biology: beyond the hope, hype and horror.

The encounter of amateur science with synthetic biology has led to the formation of several amateur/do-it-yourself biology (DIYBio) groups worldwide. Although media outlets covered DIYBio events, most seemed only to highlight the hope, hype, and horror of what DIYBio would do in the future. Here, we analyze the European amateur biology movement to find out who they are, what they aim for and how they differ from US groups. We found that all groups are driven by a core leadership of (semi-)professional people who struggle with finding lab space and equipment. Regulations on genetic modification limit what groups can do. Differences between Europe and the US are found in the distinct regulatory environments and the European emphasis on bio-art. We conclude that DIYBio Europe has so far been a responsible and transparent citizen science movement with a solid user base that will continue to grow irrespective of media attention.

Psychoactive Substances Bill and Act of New Zealand: A Chance to Engage Undergraduate Scientists with Society using a Transfer Learning Paradigm.

Our aim was to develop a teaching paradigm that connected undergraduate's neuropharmacological/toxicological knowledge to that of government policy. One goal of undergraduate education should be to help develop scientists that can use their scientific knowledge to critique government policy. There is little research, however, on whether democratization of science occurs: nor how to achieve this. Our work focused on a semi-structured workshop designed around the Psychoactive Substances Bill (PSB). Third year science students were given a questionnaire that was designed to address whether participating in the workshop enhanced their understanding of the PSB and its relationship to their established knowledge (i.e., transfer learning). Furthermore, whether they felt that they had enough expertise to consider making a submission (i.e., societal engagement). Results showed that the students appreciated the opportunity to explore potential application of their knowledge and delve into a socio-scientific issue. However, our findings suggested they felt uncomfortable discussing their ideas outside the classroom: nor, did they identify themselves as having sufficient knowledge to contribute to a submission. In conclusion, this study highlights two points. First, that discussion based transfer learning can be used in the tertiary sector and students value the opportunity to apply their knowledge to socio-scientific issue. Second, if social participation and democratization of science is a goal, then more emphasis should be placed on how students can realistically and confidently apply their learning to change social policy. In order to achieve this, education programs need to focus on legitimate real-life processes such as the PSB for engagement.