Reference genome of Townsend's big-eared bat, Corynorhinus townsendii.

Townsend's big-eared bat, Corynorhinus townsendii, is a cave- and mine-roosting species found largely in western North America. Considered a species of conservation concern throughout much of its range, protection efforts would greatly benefit from understanding patterns of population structure, genetic diversity, and local adaptation. To facilitate such research, we present the first de novo genome assembly of C. townsendii as part of the California Conservation Genomics Project (CCGP). Pacific Biosciences HiFi long reads and Omni-C chromatin-proximity sequencing technologies were used to produce a de novo genome assembly, consistent with the standard CCGP reference genome protocol. This assembly comprises 391 scaffolds spanning 2.1 Gb, represented by a scaffold N50 of 174.6 Mb, a contig N50 of 23.4 Mb, and a benchmarking universal single-copy ortholog (BUSCO) completeness score of 96.6%. This high-quality genome will be a key tool for informed conservation and management of this vulnerable species in California and across its range.

High latitude northern bats (Eptesicus nilssonii) reveal adaptations to both high and low ambient temperatures.

Insectivorous bats at northern latitudes need to cope with long periods of no food for large parts of the year. Hence, bats which are resident at northern latitudes throughout the year will need to undergo a long hibernation season and a short reproductive season where foraging time is limited by extended daylight periods. Eptesicus nilssonii is the northernmost occurring bat species worldwide and hibernates locally when ambient temperatures (Ta) limit prey availability. Therefore, we investigated the energy spent maintaining normothermy at different Ta, as well as how much bats limit energy expenditure while in torpor. We found that, despite being exposed to Ta as low as 1.1°C, bats did not increase torpid metabolic rate, thus indicating that E. nilssonii can survive and hibernate at low ambient temperatures. Furthermore, we found a lower critical temperature (Tlc) of 27.8°C, which is lower than in most other vespertilionid bats, and we found no indication of any metabolic response to Ta up to 37.1°C. Interestingly, carbon dioxide production increased with increasing Ta above the Tlc, presumably caused by a release of retained CO2 in bats that remained in torpor for longer and aroused at Ta above the Tlc. Our results indicate that E. nilssonii can thermoconform at near-freezing Ta, and hence maintain longer torpor bouts with limited energy expenditure, yet also cope with high Ta when sun exposed in roosts during long summer days. These physiological traits are likely to enable the species to cope with ongoing and predicted climate change.

Mind the gap: natural cleft palates reduce biting performance in bats.

Novel morphological traits pose interesting evolutionary paradoxes when they become widespread in a lineage while being deleterious in others. Cleft palate is a rare congenital condition in mammals in which the incisor-bearing premaxilla bones of the upper jaw develop abnormally. However, ∼50% of bat species have natural, non-pathological cleft palates. We used the family Vespertilionidae as a model and linear and geometric morphometrics within a phylogenetic framework to (1) explore evolutionary patterns in cleft morphology, and (2) test whether cleft morphological variation is correlated with skull shape in bats. We also used finite element (FE) analyses to experimentally test how presence of a cleft palate impacts skull performance during biting in a species with extreme cleft morphology (hoary bat, Lasiurus cinereus). We constructed and compared the performance of two FE models: one based on the hoary bat's natural skull morphology, and another with a digitally filled cleft simulating a complete premaxilla. Results showed that cleft length and width are correlated with skull shape in Vespertilionidae, with narrower, shallower clefts seen in more gracile skulls and broader, deeper clefts in more robust skulls. FE analysis showed that the model with a natural cleft produced lower bite forces, and had higher stress and strain than the model with a filled cleft. In the rostrum, safety factors were 1.59-2.20 times higher in the model with a filled cleft than in the natural model. Our results demonstrate that cleft palates in bats reduce biting performance, and evolution of skull robusticity may compensate for this reduction in performance.

Great Evening Bat Ia io (Vespertilionidae, Chiroptera) from the Pleistocene of Vietnam (Lang Trang Cave).

A dentary fragment of a great evening bat Ia io Thomas, 1902 found in 2020 in the Pleistocene deposits of the Lang Trang karstic cave in northern Vietnam is described. This rare species of large tropical bats now inhabits Nepal, eastern India, southern China, Thailand, Laos, and Vietnam and as a fossil is known from the Pleistocene of China. In Vietnam the fossil remains of this species have been discovered for the first time. By the total size and lower molar structure, the specimen from the Lang Trang cave is most similar to the subspecies Ia io peninsulata Soisook et al., 2017 that now occurs in southern Thailand.

Sonar strobe groups and buzzes are produced before powered flight is achieved in the juvenile big brown bat, Eptesicus fuscus.

Laryngeally echolocating bats produce a rapid succession of echolocation calls just before landing. These landing buzzes exhibit an increase in call rate and a decrease in call peak frequency and duration relative to pre-buzz calls, and resemble the terminal buzz phase calls of an aerial hawking bat's echolocation attack sequence. Sonar strobe groups (SSGs) are clustered sequences of non-buzz calls whose pulse intervals (PIs) are fairly regular and shorter than the PIs both before and after the cluster, but longer than the PIs of buzz calls. Like buzzes, SSGs are thought to indicate increased auditory attention. We recorded the echolocation calls emitted by juvenile big brown bats (Eptesicus fuscus) over postnatal development from birth to 32 days old, when full flight has normally been achieved, and tested the following hypotheses: (i) buzz production precedes the onset of controlled, powered flight; (ii) the emission of SSGs precedes buzzes and coincides with the onset of fluttering behaviour; and (iii) the onset of flight is attained first by young bats with adult-like wing loadings. We found that E. fuscus pups emitted landing buzzes before they achieved powered flight and produced SSGs several days before emitting landing buzzes. Both observations indicate that the onset of adult-like echolocation behaviour occurs prior to adult-like flight behaviour. Pups that achieved flight first were typically those that also first achieved low, adult-like wing loadings. Our results demonstrate that echolocation and flight develop in parallel but may be temporally offset, such that the sensory system precedes the locomotory system during postnatal ontogeny.

The behavioral energetics of New Zealand's bats: Daily torpor and hibernation, a continuum.

We examine the impact of behavior on the short-term energy expenditures of the only terrestrial mammals endemic to New Zealand, two bats, the long-tailed (Chalinolobus tuberculatus, family Vespertilionidae), and the lesser short-tailed (Mystacina tuberculata, family Mystacinidae). Vespertilionidae has a world-wide distribution. Mystacinidae is restricted to New Zealand, although related to five neotropical families and one in Madagascar reflecting a shared Gondwanan origin of their Noctilionoidea superfamily. Both species have highly variable body temperatures and rates of metabolism. They feed on flying insects, which requires them to be torpid in shelters during cold, wet periods. In dry weather Mystacina is active in winter at ambient temperatures as low as -1.0 °C, foraging for terrestrial invertebrates in leaf litter, even in the presence of snow, and consuming fruit, nectar, and pollen from endemic plants that bloom in winter. The behavior of Mystacina expands its presence in a cool, wet, temperate forest in a manner unlike any other bat, another example of the distinctive characteristics of the endemic New Zealand fauna. The use of torpor generally depends on a series of factors, including body mass, ambient temperature, latitude, reproductive cycle, sociality, and fat deposits. These factors result in a diversity of responses that range along a continuum from short-term torpor to hibernation.

Contrasting thermal strategies of montane Neotropical bats at high elevations.

In the Neotropics, captive vespertilionid bats substantially reduce their metabolic rate at low ambient temperatures, similar to their temperate counterparts, whereas the ability of phyllostomids to lower metabolic rate seems to be more limited, even in mountain species. Nevertheless, field data on the thermal behaviour of syntopic individuals from these two families is lacking. Consequently, we aimed to test whether torpor was more common and deeper in vesper bats compared to leaf-nosed bats by studying skin temperature (Tsk) variation in individuals experiencing the same environmental conditions at a mountain area. Bats experienced ambient temperatures below 15 °C. Average Tsk was 10 °C in Myotis oxyotus gardneri (Vespertilionidae) during the day, while Sturnira burtonlimi (Phyllostomidae) regulated diurnal Tsk above 30 °C. Constant food availability may explain why diurnal Sturnira burtonlimi pay the high energetic cost required to remain normothermic and to defend a wide Ta-Tsk gap but further studies are needed to elucidate additional strategies that may be employed by these bats to reduce the energetic demands of normothermy. Our study shows that the contrasting thermal strategies and torpor use adopted by vespertilionid insectivores and phyllostomid frugivores in captive settings also occur in free-ranging conditions, thus providing a basis to develop further studies with predictions more accurately rooted in field data.

Postnatal growth and age estimation in Scotophilus kuhlii.

Adequate postnatal growth is important for young bats to develop skilled sensory and locomotor abilities, which are highly associated with their survival once independent. This study investigated the postnatal growth and development of Scotophilus kuhlii in captivity. An empirical growth curve was established, and the postnatal growth rate was quantified to derive an age-predictive equation. By further controlling the fostering conditions of twins, the differences in the development patterns between pups that received maternal care or were hand-reared were analyzed to determine whether the latter developed in the same manner as their maternally reared counterparts. Our results indicate that both forearm length and body mass increased rapidly and linearly during the first 4 weeks, after which the growth rate gradually decreased to reach a stable level. The first flight occurred at an average age of 39 days with a mean forearm length and body mass of 92.07% and 70.52% of maternal size, respectively. The developmental pattern of hand-reared pups, although similar to that of their maternally reared twin siblings, displayed a slightly faster growth rate in the 4th and 5th weeks. The heavier body mass of hand-reared pups during the pre-fledging period may cause higher wing loading, potentially influencing the flight performance and survival of the bats once independent.

Diversification of an emerging pathogen in a biodiversity hotspot: Leptospira in endemic small mammals of Madagascar.

Biodiversity hotspots and associated endemism are ideal systems for the study of parasite diversity within host communities. Here, we investigated the ecological and evolutionary forces acting on the diversification of an emerging bacterial pathogen, Leptospira spp., in communities of endemic Malagasy small mammals. We determined the infection rate with pathogenic Leptospira in 20 species of sympatric rodents (subfamily Nesomyinae) and tenrecids (family Tenrecidae) at two eastern humid forest localities. A multilocus genotyping analysis allowed the characterization of bacterial diversity within small mammals and gave insights into their genetic relationships with Leptospira infecting endemic Malagasy bats (family Miniopteridae and Vespertilionidae). We report for the first time the presence of pathogenic Leptospira in Malagasy endemic small mammals, with an overall prevalence of 13%. In addition, these hosts harbour species of Leptospira (L. kirschneri, L. borgpetersenii and L. borgpetersenii group B) which are different from those reported in introduced rats (L. interrogans) on Madagascar. The diversification of Leptospira on Madagascar can be traced millions of years into evolutionary history, resulting in the divergence of endemic lineages and strong host specificity. These observations are discussed in relation to the relative roles of endemic vs. introduced mammal species in the evolution and epidemiology of Leptospira on Madagascar, specifically how biodiversity and biogeographical processes can shape community ecology of an emerging pathogen and lead to its diversification within native animal communities.

Polychromophilus (Haemosporida: Plasmodiidae): A review of association with bats (Mammalia, Chiroptera) and the first record in the Neotropical bat, Myotis albescens (Chiroptera, Vespertilionidae) from Colombia.

Some species within the family Plasmodiidae (Haemosporida) have been extensively studied due to their implications for human health. However, for other haemosporidians that infect wild animals the knowledge is limited. Species within the genus Polychromophilus have thus far been documented exclusively as hemoparasites of bats. Records of Polychromophilus are primarily from Africa, Europe, and Southeast Asia, with limited information available for the Americas. Here, we assessed the state of knowledge on Polychromophilus species infecting bats worldwide and searched for the presence of Polychromophilus in blood samples of neotropical bats from Colombia. We found a total of 65 records of Polychromophilus in 46 bat species belonging to the families Emballonuridae, Hipposideridae, Miniopteridae, Rhinolophidae, Rhinonycteridae, and Vespertilionidae worldwide, except for Antarctica. In the Americas, records of the genus Polychromophilus are exclusively from Vespertilionidae bats in Brazil, Colombia, the United States, and Panama. The morphological and molecular analyses of blood from 125 bats, belonging to 39 species and captured in seven localities within the departments of Arauca and Caldas (Colombia), confirmed the presence of Polychromophilus deanei in a silver-tipped myotis, Myotis albescens (Vespertilionidae). This finding represents the first morphological and molecular confirmation of P. deanei in the Americas. Additionally, it expands the knowledge on the diversity and distribution of Polychromophilus in Neotropical bats.

Phylogenetic, morphological and niche differentiation unveil new species limits for the big brown bat (Eptesicus fuscus).

Phylogeographic accounts of mammals across fragmented landscapes show high levels of genetic, morphological and ecological variation. The big brown bat (Eptesicus fuscus) widely spans mainland landmasses from Canada to Ecuador and Colombia, and the insular Caribbean through The Bahamas and Greater and Lesser Antilles. Given the distribution of E. fuscus, we hypothesized that insular lineages could represent a different species aided by isolation. We assessed species limits by capitalizing on available mitochondrial and genomic data. Novel morphological and spatial datasets were produced to examine limits phenotypically and whether ecological niches could be associated with differences between groups. Phylogenetics strongly supported the Caribbean as unique compared to the mainland. Genomic data indicated high levels of genetic structure within the Caribbean and no detectable admixture of the Caribbean with continental lineages. Similarly, the Caribbean group shows high phenotypic disparity, and niche models revealed differences in habitat suitability between groups, concordant with the phylogenetic results. This study uncovered signals of divergence supporting the Caribbean clade of E. fuscus as unique through an integrative framework. We endorse re-evaluating the taxonomic status of Caribbean big brown bats as Eptesicus dutertreus. This recognition can help promote local conservation plans for insular lineages of big brown bats.

A Novel Mastadenovirus from Nyctalus noctula Which Represents a Distinct Evolutionary Branch of Viruses from Bats in Europe.

Bats are natural hosts of a wide variety of viruses, including adenoviruses. European bats are known to carry mastadenoviruses categorized as species B (widespread in European Vespertilionidae bats) and whose taxonomy has not been clarified. We examined fecal samples from Vespertilionidae bats (five species) captured in central Russia and found that 2/12 (16%) were positive for mastadenoviruses. The partial genome of the mastadenovirus was assembled from Pipistrellus nathusii, representing the bat adenovirus species B. The complete genome (37,915 nt) of a novel mastadenovirus was assembled from Nyctalus noctula and named BatAdV/MOW15-Nn19/Quixote. Comparative studies showed significant divergence of the Quixote genome sequence from European bat mastadenoviruses, while the only known virus showing low similarity was the isolate WA3301 from an Australian bat, and together they formed a subclade that separated from other BatAdVs. Phylogenetic and comparative analysis of the protein-coding genes provided evidence that Quixote is related to a novel species within the genus Mastadenovirus, provisionally named "K" (as the next available letter for the species). Phylogenetic analyses revealed that some earlier viruses from Western European bats, for which only partial DNA polymerase genes are known, are most likely members of the tentatively named species "K". Thus, at least two species of mastadenovirus are circulating in bats throughout Europe, from western to eastern areas.

Molecular phylogenetic reconstructions identify East Asia as the cradle for the evolution of the cosmopolitan genus Myotis (Mammalia, Chiroptera).

Sequences of the mitochondrial cytochrome b (1140 bp) and nuclear Rag 2 (1148 bp) genes were used to assess the evolutionary history of the cosmopolitan bat genus Myotis, based on a worldwide sampling of over 88 named species plus 7 species with uncertain nomenclature. Phylogenetic reconstructions of this comprehensive taxon sampling show that most radiation of species occurred independently within each biogeographic region. Our molecular study supports an early divergence of species from the New World, where all Nearctic and Neotropical species plus a lineage from the Palaearctic constitute a monophyletic clade, sister to the remaining Old World taxa. The major Old World clade includes all remaining Eurasian taxa, most Oriental species, one Oceanian, and all Ethiopian species. Another lineage, including M. latirostris from Taiwan, appears at the base of these two major biogeographic clades and, because it bears nyctalodont molars, could be considered as a distinct genus. However, this molar configuration is also found in crown-group species, indicating that these dental characters are variable in the genus Myotis and may confound interpretation of the fossil record. Molecular datings suggest an origin of all recent Myotis in the early Miocene (about 21MYA with 95% highest posterior density interval 23-20MYA). This period was characterized by a global climatic cooling that reduced the availability of tropical habitats and favoured the development of more temperate vegetation. This sharp climatic change might have triggered the evolution of Myotis in the Northern continents, because Myotis ancestors seem to have been well adapted and successful in such temperate habitats. Ancestral area reconstructions based on the molecular phylogeny suggest that the eastern portion of the Asian continent was an important center of origin for the early diversification of all Myotis lineages, and involved relatively few subsequent transcontinental range expansions.

The dataset of bat (Mammalia, Chiroptera) occurrences in Ukraine collected by the Ukrainian Bat Rehabilitation Center (2011-2022).

Background: Bats are of high conservational status in most European countries. All bats are under legal protection in Ukraine and included in the national Red Data Book. However, bats remain one of the least studied groups of mammals in Ukraine. Their cryptic lifestyle limits the possibilities of direct observations and, as a result, data on bat distribution are incomplete. Wildlife rehabilitation centres accumulate a plethora of records of wild animals and those data may significantly contribute to knowledge on the species range, phenology and habitat preferences.This paper presents the data accumulated from over a decade of work by the Ukrainian Bat Rehabilitation Center (formerly The Bat Rehabilitation Center of Feldman Ecopark), the premier organisation engaged in the rescue and rehabilitation of bats across Ukraine. In addition to in-person data collected by Ukrainian Bat Rehabilitation Center staff, the Center also accepts observations of bat encounters from citizens. The Center's dataset boasts over 20,000 distinct observations, which are the subject of this paper. New information: This dataset, spanning 2011-2022, contains a total of 20,948 records of bat findings, 19,024 of which consist of records directly identified by UBRC team members. The remaining 1924 observations were provided by citizens through helpline. Data on 16 species and one subspecies have been collected. The highest number of records belongs to Nyctalusnoctula (n = 15889), followed by Eptesicusserotinus (n = 2017) and Pipistrelluskuhliilepidus (n = 2001). Less than 10% of these records have been previously published; the rest are presented in this paper for the first time. The dataset is particularly rich in information on bats in human settlements and is (to the best of the authors' knowledge) the largest dataset on bats within human-modified landscapes ever collected from the territory of Eastern Europe. The entire dataset is available through the Global Biodiversity Information Facility (GBIF).

Metabarcoding dietary analysis in the insectivorous bat Nyctalusleisleri and implications for conservation.

In this study, we aim to uncover diet preferences for the insectivorous bat Nyctalusleisleri (Leisler's bat, the lesser noctule) and to provide recommendations for conservation of the species, based on the analysis of prey source habitats. Using a novel guano trap, we sampled bat faeces at selected roosts in a forest in Germany and tested two mitochondrial markers (COI and 16S) and three primer pairs for the metabarcoding of bat faecal pellets. We found a total of 17 arthropod prey orders comprising 358 species in N.leisleri guano. The most diverse orders were Lepidoptera (126 species), Diptera (86 species) and Coleoptera (48 species), followed by Hemiptera (28 species), Trichoptera (16 species), Neuroptera (15 species) and Ephemeroptera (10 species), with Lepidoptera species dominating in spring and Diptera in summer. Based on the ecological requirements of the most abundant arthropod species found in the bat guano, we propose some recommendations for the conservation of N.leisleri that are relevant for other insectivorous bat species.

Mammals in Tawny Owl (Strixaluco) pellets from Kharkiv Region, Ukraine.

Background: The Tawny owl (Strixaluco) is a common owl species in Europe, demonstrating generalist diet strategy. Its main prey are small rodents and numerous studies show that the composition of its diet broadly reflects changes in prey species abundance in its habitats. Due to strictly sedentary habits of adult birds and their year-round territoriality, it is possible to locate habitats of their prey with a precision of several hundred metres. Analysis of owl pellets is a traditional method in faunistic studies to provide data on distribution of small mammals, especially cryptic species which are hard to be found using trapping. New information: Here, we present a dataset on mammals found in Tawny owl pellets collected during up to 13-year studies in the Kharkiv Region, Ukraine in three territories. Data from two territories were collected in a systematic way and allowed us to make analysis of seasonal, year-to-year and habitat variability in the Tawny owl diet and local mammal species composition.

State dependence of arousal from torpor in brown long-eared bats (Plecotus auritus).

To cope with periods of low food availability and unsuitable environmental conditions (e.g., short photoperiod or challenging weather), many heterothermic mammals can readily go into torpor to save energy. However, torpor also entails several potential costs, and quantitative energetics can, therefore, be influenced by the individual state, such as available energy reserves. We studied the thermal energetics of brown long-eared bats (Plecotus auritus) in the northern part of its distributional range, including torpor entry, thermoregulatory ability during torpor and how they responded metabolically to an increasing ambient temperature (Ta) during arousal from torpor. Torpor entry occurred later in bats with higher body mass (Mb). During torpor, only 10 out of 21 bats increased oxygen consumption (V̇O2) to a greater extent above the mean torpor metabolic rates (TMR) when exposed to low Ta. The slope of the torpid thermoregulatory curve was shallower than that of resting metabolic rate (RMR) during normothermic conditions, indicating a higher thermal insulation during torpor. During exposure to an increasing Ta, all bats increased metabolic rate exponentially, but the bats with higher Mb aroused at a lower Ta than those with lower Mb. In bats with low Mb, arousal was postponed to an Ta above the lower critical temperature of the thermoneutral zone. Our results demonstrate that physiological traits, which are often considered fixed, can be more flexible than previously assumed and vary with individual state. Thus, future studies of thermal physiology should to a greater extent take individual state-dependent effects into account.

Lack of Detection of Toxoplasma gondii in Pipistrellus spp. Bats from Densely Cat-Populated Areas of NE Spain.

Toxoplasma gondii infection in healthy animals is often asymptomatic. However, some species with little history of contact with the parasite, such as marsupials and New World primates, present high mortality rates after infection. Despite its potential conservation concern, T. gondii infection in insectivorous bats has received little attention, and its impact on bat populations' health is unknown. To assess the putative role of insectivorous bats in the cycle of T. gondii, samples of three species of bats (Pipistrellus pipistrellus, P. pygmaeus and P. kuhlii) collected between 2019 and 2021 in NE Spain were tested for the presence of the parasite using a qPCR. All tissues resulted negative (0.0% prevalence with 95% CI: [0.0-2.6]) for the presence of T. gondii. Unlike previous studies on insectivorous bats from Europe, Asia and America, the present study suggests that Pipistrellus spp. bats do not play a significant role in the epidemiology of T. gondii in NE Spain. Further studies are encouraged to elucidate both the epidemiology of T. gondii and its potential impact on the health of microchiropteran species in Europe.

The Role of Molossidae and Vespertilionidae in Shaping the Diversity of Alphacoronaviruses in the Americas.

Bats are reservoirs of diverse coronaviruses (CoVs), including progenitors of severe acute respiratory syndrome CoV (SARS-CoV) and SARS-CoV-2. In the Americas, there is a contrast between alphacoronaviruses (alphaCoVs) and betaCoVs: while cospeciation prevails in the latter, alphaCoV evolution is dominated by deep and recent host switches. AlphaCoV lineages are maintained by two different bat family groups, Phyllostomidae and Vespertilionidae plus Molossidae. In this study, we used a Bayesian framework to analyze the process of diversification of the lineages maintained by Molossidae and Vespertilionidae, adding novel CoV sequences from Argentina. We provide evidence that the observed CoV diversity in these two bat families is shaped by their geographic distribution and that CoVs exhibit clustering at the level of bat genera. We discuss the causes of the cocirculation of two independent clades in Molossus and Tadarida as well as the role of Myotis as the ancestral host and a major evolutionary reservoir of alphaCoVs across the continent. Although more CoV sampling efforts are needed, these findings contribute to a better knowledge of the diversity of alphaCoVs and the links between bat host species. IMPORTANCE Bats harbor the largest diversity of coronaviruses among mammals. In the Americas, seven alphacoronavirus lineages circulate among bats. Three of these lineages are shared by members of two bat families: Vespertilionidae and Molossidae. Uncovering the relationships between these coronaviruses can help us to understand patterns of cross-species transmission and, ultimately, which hosts are more likely to be involved in spillover events. We found that two different lineages cocirculate among the bat genera Molossus and Tadarida, which share roosts and have common viral variants. The bat genus Myotis functions as a reservoir of coronavirus diversity and, as such, is a key host. Although there were some spillovers recorded, there is a strong host association, showing that once a successful host jump takes place, it is transmitted onward to members of the same bat genus.

The complete mitochondrial genome of the far Eastern myotis: Myotis bombinus Thomas, 1906 in mainland of Korea (Chiroptera, Vespertilionidae).

We have determined the second mitochondrial genome of Myotis bombinus Thomas, 1906 in mainland of Korea. The circular mitogenome of M. bombinus is 17,035 bp long which is slightly shorter than that of the previous mitogenome of M. bombinus. It includes 13 protein-coding genes (PCGs), two ribosomal RNA genes, and 22 transfer RNAs. The base composition was AT-biased (66.1%). Fifty single nucleotide polymorphisms and 14 insertions were identified between two mitogenomes of M. bombinus. Phylogenetic trees show that both M. bominus mitogenomes are clustered in one clade.