RESUMO
The actomyosin complex plays crucial roles in various life processes by balancing the forces generated by cellular components. In addition to its physical function, the actomyosin complex participates in mechanotransduction. However, the exact role of actomyosin contractility in force transmission and the related transcriptional changes during morphogenesis are not fully understood. Here, we report a mechanogenetic role of the actomyosin complex in branching morphogenesis using an organotypic culture system of mouse embryonic submandibular glands. We dissected the physical factors arranged by characteristic actin structures in developing epithelial buds and identified the spatial distribution of forces that is essential for buckling mechanism to promote the branching process. Moreover, the crucial genes required for the distribution of epithelial progenitor cells were regulated by YAP and TAZ through a mechanotransduction process in epithelial organs. These findings are important for our understanding of the physical processes involved in the development of epithelial organs and provide a theoretical background for developing new approaches for organ regeneration.
Assuntos
Citoesqueleto de Actina/genética , Actomiosina/genética , Morfogênese/genética , Contração Muscular/genética , Citoesqueleto de Actina/ultraestrutura , Actinas/genética , Actinas/ultraestrutura , Actomiosina/ultraestrutura , Aciltransferases/genética , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Células Epiteliais/metabolismo , Epitélio/crescimento & desenvolvimento , Epitélio/metabolismo , Humanos , Mecanotransdução Celular/genética , Camundongos , Regeneração/genética , Glândula Submandibular/metabolismo , Proteínas de Sinalização YAPRESUMO
The interaction of myosin with actin filaments is the central feature of muscle contraction and cargo movement along actin filaments of the cytoskeleton. The energy for these movements is generated during a complex mechanochemical reaction cycle. Crystal structures of myosin in different states have provided important structural insights into the myosin motor cycle when myosin is detached from F-actin. The difficulty of obtaining diffracting crystals, however, has prevented structure determination by crystallography of actomyosin complexes. Thus, although structural models exist of F-actin in complex with various myosins, a high-resolution structure of the F-actinmyosin complex is missing. Here, using electron cryomicroscopy, we present the structure of a human rigor actomyosin complex at an average resolution of 3.9 Å. The structure reveals details of the actomyosin interface, which is mainly stabilized by hydrophobic interactions. The negatively charged amino (N) terminus of actin interacts with a conserved basic motif in loop 2 of myosin, promoting cleft closure in myosin. Surprisingly, the overall structure of myosin is similar to rigor-like myosin structures in the absence of F-actin, indicating that F-actin binding induces only minimal conformational changes in myosin. A comparison with pre-powerstroke and intermediate (Pi-release) states of myosin allows us to discuss the general mechanism of myosin binding to F-actin. Our results serve as a strong foundation for the molecular understanding of cytoskeletal diseases, such as autosomal dominant hearing loss and diseases affecting skeletal and cardiac muscles, in particular nemaline myopathy and hypertrophic cardiomyopathy.
Assuntos
Actomiosina/química , Actomiosina/ultraestrutura , Citoplasma/química , Actinas/química , Actinas/ultraestrutura , Sítios de Ligação , Microscopia Crioeletrônica , Humanos , Modelos Moleculares , Miosinas/química , Miosinas/ultraestrutura , Ligação Proteica , Conformação Proteica , Estabilidade ProteicaRESUMO
Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.
Assuntos
Citoesqueleto de Actina/ultraestrutura , Actinas/ultraestrutura , Músculo Estriado/ultraestrutura , Troponina/ultraestrutura , Actinas/química , Actomiosina/química , Actomiosina/ultraestrutura , Animais , Cálcio , Microscopia Crioeletrônica , Camundongos , Sarcômeros/química , Sarcômeros/ultraestrutura , Tropomiosina/ultraestrutura , Troponina/químicaRESUMO
After several decades studying different acto-myosin complexes at lower and intermediate resolution - limited by the electron microscope instrumentation available then - recent advances in imaging technology have been crucial for obtaining a number of excellent high-resolution 3D reconstructions from cryo electron microscopy. The resolution level reached now is about 3-4 Å, which allows unambiguous model building of filamentous actin on its own as well as that of actin filaments decorated with strongly bound myosin variants. The interface between actin and the myosin motor domain can now be described in detail, and the function of parts of the interface (such as, e.g., the cardiomyopathy loop) can be understood in a mechanistical way. Most recently, reconstructions of actin filaments decorated with different myosins, which show a strongly bound acto-myosin complex also in the presence of the nucleotide ADP, have become available. The comparison of these structures with the nucleotide-free Rigor state provide the first mechanistic description of force sensing. An open question is still the initial interaction of the motor domain of myosin with the actin filament. Such weakly interacting states have so far not been the subject of microscopical studies, even though high-resolution structures would be needed to shed light on the initial steps of phosphate release and power stroke initiation.
Assuntos
Actomiosina/química , Citoesqueleto de Actina , Actinas/química , Actomiosina/ultraestrutura , Microscopia Crioeletrônica , Miosinas/químicaRESUMO
It is not known which kinetic step in the acto-myosin ATPase cycle limits contraction speed in unloaded muscles (V0). Huxley's 1957 model [Huxley AF (1957) Prog Biophys Biophys Chem 7:255-318] predicts that V0 is limited by the rate that myosin detaches from actin. However, this does not explain why, as observed by Bárány [Bárány M (1967) J Gen Physiol 50(6, Suppl):197-218], V0 is linearly correlated with the maximal actin-activated ATPase rate (vmax), which is limited by the rate that myosin attaches strongly to actin. We have observed smooth muscle myosin filaments of different length and head number (N) moving over surface-attached F-actin in vitro. Fitting filament velocities (V) vs. N to a detachment-limited model using the myosin step size d=8 nm gave an ADP release rate 8.5-fold faster and ton (myosin's attached time) and r (duty ratio) â¼10-fold lower than previously reported. In contrast, these data were accurately fit to an attachment-limited model, V=N·v·d, over the range of N found in all muscle types. At nonphysiologically high N, V=L/ton rather than d/ton, where L is related to the length of myosin's subfragment 2. The attachment-limited model also fit well to the [ATP] dependence of V for myosin-rod cofilaments at three fixed N. Previously published V0 vs. vmax values for 24 different muscles were accurately fit to the attachment-limited model using widely accepted values for r and N, giving d=11.1 nm. Therefore, in contrast with Huxley's model, we conclude that V0 is limited by the actin-myosin attachment rate.
Assuntos
Citoesqueleto de Actina/metabolismo , Actomiosina/metabolismo , Contração Muscular , Miosinas/metabolismo , Citoesqueleto de Actina/química , Citoesqueleto de Actina/ultraestrutura , Actinas/química , Actinas/metabolismo , Actinas/ultraestrutura , Actomiosina/química , Actomiosina/ultraestrutura , Trifosfato de Adenosina/metabolismo , Trifosfato de Adenosina/farmacologia , Algoritmos , Animais , Galinhas , Cinética , Microscopia Eletrônica , Microscopia de Fluorescência/métodos , Modelos Biológicos , Músculo Liso/metabolismo , Miosinas/química , Miosinas/ultraestrutura , Ligação Proteica/efeitos dos fármacos , Coelhos , Rodaminas/químicaRESUMO
Half a century has passed since the cross-bridge structure was recognized as the molecular machine that generates muscle tension. Despite various approaches by a number of scientists, information on the structural changes in the myosin heads, particularly its transient configurations, remains scant even now, in part because of their small size and rapid stochastic movements during the power stroke. Though progress in cryo-electron microscopy is eagerly awaited as the ultimate means to elucidate structural details, the introduction of some unconventional methods that provide high-contrast raw images of the target protein assemblies is quite useful, if available, to break the current impasse. Quick-freeze deepâ»etchâ»replica electron microscopy coupled with dedicated image analysis procedures, and high-speed atomic-force microscopy are two such candidates. We have applied the former to visualize actin-associated myosin heads under in vitro motility assay conditions, and found that they take novel configurations similar to the SH1â»SH2-crosslinked myosin that we characterized recently. By incorporating biochemical and biophysical results, we have revised the cross-bridge mechanism to involve the new conformer as an important main player. The latter “microscopy” is unique and advantageous enabling continuous observation of various protein assemblies as they function. Direct observation of myosin-V’s movement along actin filaments revealed several unexpected behaviors such as foot-stomping of the leading head and unwinding of the coiled-coil tail. The potential contribution of these methods with intermediate spatial resolution is discussed.
Assuntos
Actomiosina/ultraestrutura , Tono Muscular , Músculo Esquelético/ultraestrutura , Miosinas/ultraestrutura , Actomiosina/química , Microscopia Crioeletrônica , Humanos , Microscopia de Força Atômica , Miosinas/químicaRESUMO
Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. Fesselin or synaptopodin 2 appears to be another such protein. Rapid kinetic measurements and electron microscopy demonstrated that fesselin, isolated from turkey gizzard muscle, reduced the rate of dissociation of myosin filaments. Addition of fesselin increased both the length and thickness of myosin filaments. The rate of detachment of myosin, but not heavy meromyosin, from actin was also greatly reduced by fesselin. Data from this study suggest that fesselin stabilizes myosin filaments and tethers myosin to actin. These results support the view that one role of fesselin is to organize contractile units of myosin and actin.
Assuntos
Actinas/química , Actomiosina/química , Trifosfato de Adenosina/metabolismo , Proteínas Aviárias/química , Citoesqueleto/química , Proteínas de Membrana/química , Proteínas dos Microfilamentos/química , Miosinas de Músculo Liso/química , Actinas/metabolismo , Actinas/ultraestrutura , Actomiosina/metabolismo , Actomiosina/ultraestrutura , Animais , Proteínas Aviárias/isolamento & purificação , Proteínas Aviárias/metabolismo , Proteínas Aviárias/ultraestrutura , Citoesqueleto/metabolismo , Citoesqueleto/ultraestrutura , Moela das Aves , Cinética , Proteínas de Membrana/isolamento & purificação , Proteínas de Membrana/metabolismo , Proteínas de Membrana/ultraestrutura , Proteínas dos Microfilamentos/isolamento & purificação , Proteínas dos Microfilamentos/metabolismo , Proteínas dos Microfilamentos/ultraestrutura , Microscopia Eletrônica de Transmissão , Músculo Liso/metabolismo , Subfragmentos de Miosina/química , Subfragmentos de Miosina/isolamento & purificação , Subfragmentos de Miosina/metabolismo , Subfragmentos de Miosina/ultraestrutura , Estabilidade Proteica , Coelhos , Vesículas Secretórias/metabolismo , Vesículas Secretórias/ultraestrutura , Miosinas de Músculo Liso/isolamento & purificação , Miosinas de Músculo Liso/metabolismo , Miosinas de Músculo Liso/ultraestrutura , PerusAssuntos
Núcleo Celular/ultraestrutura , Citocinese , Citometria de Fluxo/normas , Ploidias , Actomiosina/metabolismo , Actomiosina/ultraestrutura , Linhagem Celular , Núcleo Celular/metabolismo , Fase G1/genética , Expressão Gênica , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Células HeLa , Humanos , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Proteína Vermelha FluorescenteRESUMO
In the early embryo, the brain initially forms as a relatively straight, cylindrical epithelial tube composed of neural stem cells. The brain tube then divides into three primary vesicles (forebrain, midbrain, hindbrain), as well as a series of bulges (rhombomeres) in the hindbrain. The boundaries between these subdivisions have been well studied as regions of differential gene expression, but the morphogenetic mechanisms that generate these constrictions are not well understood. Here, we show that regional variations in actomyosin-based contractility play a major role in vesicle formation in the embryonic chicken brain. In particular, boundaries did not form in brains exposed to the nonmuscle myosin II inhibitor blebbistatin, whereas increasing contractile force using calyculin or ATP deepened boundaries considerably. Tissue staining showed that contraction likely occurs at the inner part of the wall, as F-actin and phosphorylated myosin are concentrated at the apical side. However, relatively little actin and myosin was found in rhombomere boundaries. To determine the specific physical mechanisms that drive vesicle formation, we developed a finite-element model for the brain tube. Regional apical contraction was simulated in the model, with contractile anisotropy and strength estimated from contractile protein distributions and measurements of cell shapes. The model shows that a combination of circumferential contraction in the boundary regions and relatively isotropic contraction between boundaries can generate realistic morphologies for the primary vesicles. In contrast, rhombomere formation likely involves longitudinal contraction between boundaries. Further simulations suggest that these different mechanisms are dictated by regional differences in initial morphology and the need to withstand cerebrospinal fluid pressure. This study provides a new understanding of early brain morphogenesis.
Assuntos
Actomiosina/análise , Actomiosina/metabolismo , Encéfalo/embriologia , Embrião de Galinha/embriologia , Actomiosina/ultraestrutura , Animais , Encéfalo/citologia , Encéfalo/metabolismo , Encéfalo/ultraestrutura , Forma Celular , Embrião de Galinha/citologia , Embrião de Galinha/metabolismo , Embrião de Galinha/ultraestrutura , Modelos BiológicosRESUMO
Tight regulation of the contractility of the actomyosin cortex is essential for proper cell locomotion and division. Enhanced contractility leads, for example, to aberrations in the positioning of the mitotic spindle or to anomalous migration modes that allow tumor cells to escape anti-dissemination treatments. Spherical membrane protrusions called blebs occasionally appear during cell migration, cell division or apoptosis. We have shown that the cortex ruptures at sites where actomyosin cortical contractility is increased, leading to the formation of blebs. Here, we propose that bleb formation, which releases cortical tension, can be used as a reporter of cortical contractility. We go on to analyze the implications of spontaneous cortical contractile behaviors on cell locomotion and division and we particularly emphasize that variations in actomyosin contractility can account for a variety of migration modes.
Assuntos
Actomiosina/análise , Actomiosina/fisiologia , Divisão Celular/fisiologia , Movimento Celular/fisiologia , Citoplasma/química , Actomiosina/ultraestrutura , Animais , Membrana Celular/química , Membrana Celular/fisiologia , Membrana Celular/ultraestrutura , Polaridade Celular , Proteínas Contráteis/análise , Proteínas Contráteis/fisiologia , Proteínas Contráteis/ultraestrutura , Citocinese , Citoplasma/ultraestrutura , Proteínas do Citoesqueleto/análise , Proteínas do Citoesqueleto/fisiologia , Proteínas do Citoesqueleto/ultraestrutura , Citoesqueleto/química , Citoesqueleto/fisiologia , Citoesqueleto/ultraestrutura , Géis , HumanosRESUMO
The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.
Assuntos
Actomiosina/ultraestrutura , Miosina Tipo V/ultraestrutura , Actinas/metabolismo , Animais , Fenômenos Biomecânicos , Galinhas , Humanos , Ligação Proteica , CoelhosRESUMO
Actomyosin-undercoated adherens junctions are critical for epithelial cell integrity and remodeling. Actomyosin associates with adherens junctions through αE-catenin complexed with ß-catenin and E-cadherin in vivo; however, in vitro biochemical studies in solution showed that αE-catenin complexed with ß-catenin binds to F-actin less efficiently than αE-catenin that is not complexed with ß-catenin. Although a "catch-bond model" partly explains this inconsistency, the mechanism for this inconsistency between the in vivo and in vitro results remains elusive. We herein demonstrate that afadin binds to αE-catenin complexed with ß-catenin and enhances its F-actin-binding activity in a novel mechanism, eventually inducing the proper actomyosin organization through αE-catenin complexed with ß-catenin and E-cadherin at adherens junctions.
Assuntos
Junções Aderentes/genética , Caderinas/genética , Proteínas dos Microfilamentos/genética , beta Catenina/genética , Citoesqueleto de Actina/genética , Citoesqueleto de Actina/ultraestrutura , Actinas/genética , Actomiosina/genética , Actomiosina/ultraestrutura , Junções Aderentes/ultraestrutura , Animais , Humanos , Camundongos , Camundongos Knockout , Complexos Multiproteicos/genética , Complexos Multiproteicos/ultraestrutura , Ligação Proteica/genética , Vinculina/genética , alfa Catenina/genética , alfa Catenina/ultraestruturaRESUMO
Image analysis of electron micrographs of thin-sectioned myosin subfragment-1 (S1) crystals has been used to determine the structure of the myosin head at approximately 25-A resolution. Previous work established that the unit cell of type I crystals of myosin S1 contains eight molecules arranged with orthorhombic space group symmetry P212121 and provided preliminary information on the size and shape of the myosin head (Winkelmann, D. A., H. Mekeel, and I. Rayment. 1985. J. Mol. Biol. 181:487-501). We have applied a systematic method of data collection by electron microscopy to reconstruct the three-dimensional (3D) structure of the S1 crystal lattice. Electron micrographs of thin sections were recorded at angles of up to 50 degrees by tilting the sections about the two orthogonal unit cell axes in sections cut perpendicular to the three major crystallographic axes. The data from six separate tilt series were merged to form a complete data set for 3D reconstruction. This approach has yielded an electron density map of the unit cell of the S1 crystals of sufficient detail. to delineate the molecular envelope of the myosin head. Myosin S1 has a tadpole-shaped molecular envelope that is very similar in appearance to the pear-shaped myosin heads observed by electron microscopy of rotary-shadowed and negatively stained myosin. The molecule is divided into essentially three morphological domains: a large domain on one end of the molecule corresponding to approximately 60% of the total molecular volume, a smaller central domain of approximately 30% of the volume that is separated from the larger domain by a cleft on one side of the molecule, and the smallest domain corresponding to a thin tail-like region containing approximately 10% of the volume. This molecular organization supports models of force generation by myosin which invoke conformational mobility at interdomain junctions within the head.
Assuntos
Subfragmentos de Miosina/ultraestrutura , Actomiosina/ultraestrutura , Animais , Galinhas , Microscopia Eletrônica , Modelos Moleculares , Músculos , Subfragmentos de Miosina/isolamento & purificação , Miosinas/ultraestrutura , Conformação ProteicaRESUMO
To identify regulatory mechanisms potentially involved in formation of actomyosin structures in smooth muscle cells, the influence of F-actin on smooth muscle myosin assembly was examined. In physiologically relevant buffers, AMPPNP binding to myosin caused transition to the soluble 10S myosin conformation due to trapping of nucleotide at the active sites. The resulting 10S myosin-AMPPNP complex was highly stable and thick filament assembly was suppressed. However, upon addition to F-actin, myosin readily assembled to form thick filaments. Furthermore, myosin assembly caused rearrangement of actin filament networks into actomyosin fibers composed of coaligned F-actin and myosin thick filaments. Severin-induced fragmentation of actin in actomyosin fibers resulted in immediate disassembly of myosin thick filaments, demonstrating that actin filaments were indispensable for mediating myosin assembly in the presence of AMPPNP. Actomyosin fibers also formed after addition of F-actin to nonphosphorylated 10S myosin monomers containing the products of ATP hydrolysis trapped at the active site. The resulting fibers were rapidly disassembled after addition of millimolar MgATP and consequent transition of myosin to the soluble 10S state. However, reassembly of myosin filaments in the presence of MgATP and F-actin could be induced by phosphorylation of myosin P-light chains, causing regeneration of actomyosin fiber bundles. The results indicate that actomyosin fibers can be spontaneously formed by F-actin-mediated assembly of smooth muscle myosin. Moreover, induction of actomyosin fibers by myosin light chain phosphorylation in the presence of actin filament networks provides a plausible hypothesis for contractile fiber assembly in situ.
Assuntos
Actinas/metabolismo , Actomiosina/metabolismo , Músculo Liso/metabolismo , Miosinas/metabolismo , Proteínas de Protozoários , Actomiosina/ultraestrutura , Difosfato de Adenosina/metabolismo , Adenilil Imidodifosfato/metabolismo , Animais , Galinhas , Proteínas Fúngicas/farmacologia , Cinética , Proteínas dos Microfilamentos/farmacologia , Microscopia Eletrônica , FosforilaçãoRESUMO
The interaction between myosin subfragment 1 (S1) and actin filaments after the photolysis of P3-1-(2-nitrophenyl)ethyl ester of ATP (caged ATP) was analyzed with a newly developed freezing system using liquid helium. Actin and S1 (100 microM each) formed a ropelike double-helix characteristic of rigor in the presence of 5 mM caged ATP at room temperature. At 15 ms after photolysis, the ropelike double helix was partially disintegrated. The number of S1 attached to actin filaments gradually decreased up to 35 ms after photolysis, and no more changes were detected from 35 to 200 ms. After depletion of ATP, the ropelike double helix was reformed. Taking recent analyses of actomyosin kinetics into consideration, we concluded that most S1 observed on actin filaments at 35-200 ms are so called "weakly bound S1" (S1.ATP or S1.ADP.Pi) and that the weakly bound S1 under a rapid association-dissociation equilibrium with actin filaments can be captured by electron microscopy by means of our newly developed freezing system. This enabled us to directly compare the conformation of weakly and strongly bound S1. Within the resolution of deep-etch replica technique, there were no significant conformational differences between weakly and strongly bound S1, and neither types of S1 showed any positive cooperativity in their binding to actin filaments. Close comparison revealed that the weakly and strongly bound S1 have different angles of attachment to actin filaments. As compared to strongly bound S1, weakly bound S1 showed a significantly broader distribution of attachment angles. These results are discussed with special reference to the molecular mechanism of acto-myosin interaction in the presence of ATP.
Assuntos
Actinas/ultraestrutura , Actomiosina/ultraestrutura , Subfragmentos de Miosina/metabolismo , Miosinas/ultraestrutura , Citoesqueleto de Actina/ultraestrutura , Trifosfato de Adenosina/metabolismo , Animais , Simulação por Computador , Técnica de Congelamento e Réplica , Técnicas In Vitro , Substâncias Macromoleculares , Microscopia Eletrônica , Fotólise , Ligação Proteica , CoelhosRESUMO
Myosin is one of only three proteins known to convert chemical energy into mechanical work. Although the chemical, kinetic and physiological characteristics of this protein have been studied extensively, it has been difficult to define its molecular basis of movement. With the recent X-ray structural determination of the myosin head, however, it is now possible to put forward a hypothesis on how myosin might function as a molecular motor.
Assuntos
Miosinas/ultraestrutura , Actomiosina/fisiologia , Actomiosina/ultraestrutura , Modelos Moleculares , Miosinas/fisiologia , Relação Estrutura-AtividadeRESUMO
Lysenin, a toxin present in the coelomic fluid of the earthworm Eisenia foetida, is known to cause a long-lasting contraction of rat aorta smooth muscle strips. We addressed the mechanisms underlying its action on smooth muscle cells and present the first report demonstrating a completely new property of lysenin unrelated to its basic sphingomyelin-binding ability. Here we report lysenin enhancement effect on smooth muscle actomyosin ATPase activity and the ability of networking the actin filaments. The maximum enhancement of the ATPase activity of actomyosin at 120 mM KCl was observed at a molar ratio of lysenin to actin of about 1:10(5), while at 70 mM KCl at the ratio of about 1:10(6). The effect of lysenin became most pronounced only when both smooth muscle regulatory proteins, tropomyosin and caldesmon, were present. Co-sedimentation experiments indicated that lysenin did not displace neither tropomyosin nor caldesmon from the thin filament. Thus, the lysenin-dependent abolishment of the inhibitory effect of caldesmon on the ATPase activity was related rather to the modification of the filament structure. The ability of the toxin to exert its stimulatory effect at extremely low concentrations (as low as one molecule of lysenin per 10(6) actin molecules) may result from the long-range cooperative transitions in the entire thin filament with an involvement of smooth muscle tropomyosin, while the role of caldesmon may be limited exclusively to the inhibition of ATPase activity.
Assuntos
Actomiosina/metabolismo , Contração Muscular/efeitos dos fármacos , Músculo Liso/metabolismo , Oligoquetos , Toxinas Biológicas/toxicidade , Citoesqueleto de Actina/química , Citoesqueleto de Actina/metabolismo , Citoesqueleto de Actina/ultraestrutura , Actomiosina/química , Actomiosina/ultraestrutura , Adenosina Trifosfatases/metabolismo , Animais , Eletroforese em Gel de Poliacrilamida , Microscopia Eletrônica , Contração Muscular/fisiologia , Músculo Liso/química , Músculo Liso/ultraestrutura , RatosRESUMO
New electron microscopic data provide direct evidence in support of the classic steric-blocking model for regulation of actin-myosin interactions by tropomyosin.
Assuntos
Actomiosina/ultraestrutura , Microscopia Eletrônica , Modelos Biológicos , Modelos Moleculares , Conformação Proteica , Tropomiosina/ultraestrutura , Actomiosina/metabolismo , Regulação Alostérica , Animais , Cálcio/metabolismo , Caranguejos Ferradura , Insetos , Ranidae , Tropomiosina/metabolismoRESUMO
Cytokinesis is an essential cellular event that completes the cell division cycle. It begins with the assembly of an actomyosin contractile ring that undergoes constriction concomitant with the septum formation to divide the cell in two. Placement of the septum at the right position is important to ensure fidelity of the division process. In fission yeast, the medially placed nucleus is a major spatial cue to position the site of division. In this chapter, we describe a simple synthetic biology-based approach to displace the nucleus and study the consequence on division site positioning. We also describe how to perform fluorescence recovery after photobleaching to follow the dynamics of cytokinetic proteins at defined time points by live-cell microscopy.
Assuntos
Núcleo Celular/ultraestrutura , Citocinese/genética , Recuperação de Fluorescência Após Fotodegradação/métodos , Schizosaccharomyces/genética , Citoesqueleto de Actina/ultraestrutura , Actomiosina/genética , Actomiosina/ultraestrutura , Núcleo Celular/genética , Microscopia/métodos , Schizosaccharomyces/ultraestruturaRESUMO
The tension generated by randomly distributed myosin minifilaments in an actin gel is evaluated using a rigorous theorem relating the surface forces acting on the gel to the forces exerted by the myosins. The maximum tension generated per myosin depends strongly on the lengths of the myosin minifilaments and the actin filaments. The result is used to place an upper bound on the tension that can be generated during cytokinesis. It is found that actomyosin contraction by itself generates too little force for ring contraction during cytokinesis unless the actin filaments are tightly crosslinked into inextensible units much longer than a single actin filament.