RESUMO
Natural product congeners serve a useful role in the understanding of natural product biosynthesis and structure-activity relationships. A minor congener with superior activity, selectivity, and modifiable functional groups could serve as a more effective lead structure and replace even the original lead molecule that was used for medicinal chemistry modifications. Currently, no effective method exists to discover targeted congeners rapidly, specifically, and selectively from producing sources. Herein, a new method based on liquid-chromatography tandem-mass spectrometry combination is evaluated for targeted discovery of congeners of platensimycin and platencin from the extracts of Streptomyces platensis. By utilizing a precursor-ion searching protocol, tandem mass spectrometry not only confirmed the presence of known congeners but also provided unambiguous detection of many previously unknown congeners of platensimycin and platencin. This high-throughput and quantitative method can be rapidly and broadly applied for dereplication and congener discovery from a variety of producing sources, even when the targeted compounds are obscured by the presence of unrelated natural products.
Assuntos
Adamantano/química , Aminobenzoatos/química , Aminofenóis/química , Anilidas/química , Ensaios de Triagem em Larga Escala/métodos , Compostos Policíclicos/química , Streptomyces/química , Adamantano/isolamento & purificação , Aminobenzoatos/isolamento & purificação , Aminofenóis/isolamento & purificação , Anilidas/isolamento & purificação , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Cromatografia Líquida , Estrutura Molecular , Compostos Policíclicos/isolamento & purificação , Relação Estrutura-Atividade , Espectrometria de Massas em TandemRESUMO
The enantiomers of vildagliptin, an orally available and selective dipeptidyl-peptidase-4 inhibitor used for the treatment of type II diabetes, have been separated by CD-modified CZE, using uncoated fused-silica capillary. After screening 13 negatively charged CD derivatives as potential chiral selectors, sulfobutyl-ether-α-CD (SBE-α-CD) was selected for the enantioseparation. For the optimization, a factorial analysis study was performed by orthogonal experimental design. Six experimental factors were chosen as variable parameters: temperature, applied voltage, chiral selector and BGE concentrations, pH, and the parameters of the hydrodynamic injection. The optimized system still was not considered final as the second peak (S-enantiomer) migrated too close to the EOF, resulting in a potential inaccuracy during the determination of the chiral impurity. To fine-tune the method "one factor at a time" variation approach was applied. The final method (applying 15°C capillary temperature, 40 mbar × 4 s hydrodynamic injection, 25 kV voltage in 75 mM acetate-Tris buffer [pH 4.75] containing 20 mM SBE-α-CD as chiral selector) was validated according to the ICH guideline. RSD percentage of the resolution value, migration times, and corrected peak areas were below 5% during testing repeatability and intermediate precision. LOD and LOQ values were found to be 2.5 and 7.5 µg/mL, respectively. The method is considered linear in the 7.5-180 µg/mL range for the R-enantiomer. The robustness of the method was justified using Plackett-Burmann statistical experimental design.
Assuntos
Adamantano/análogos & derivados , Ciclodextrinas/química , Eletroforese Capilar/métodos , Hipoglicemiantes/química , Nitrilas/isolamento & purificação , Pirrolidinas/isolamento & purificação , Adamantano/química , Adamantano/isolamento & purificação , Diabetes Mellitus Tipo 2/tratamento farmacológico , Concentração de Íons de Hidrogênio , Limite de Detecção , Nitrilas/química , Pirrolidinas/química , Reprodutibilidade dos Testes , Estereoisomerismo , Temperatura , VildagliptinaRESUMO
In this work, a miniaturized solid-phase extraction (SPE) platform, called sorbent membrane funnel, which permits in situ cleanup prior to membrane funnel-based spray analysis was developed. The fabrication of funnel and the mounting of SPE sorbent were simple and straightforward by a homemade punching system. Using different sorbents, the SPE sorbent funnel has been successfully applied in spray analysis of drug molecules spiked in human plasma, trypsin digested solution of bovine serum albumin in the presence of high concentration of chaotropic reagents, and phosphopeptides in the tryptic digested solution of casein. The results demonstrated that SPE sorbent attached membrane funnels can be a useful tool in common metabolomic and proteomic applications.
Assuntos
Membranas Artificiais , Miniaturização/métodos , Extração em Fase Sólida/métodos , Adamantano/análogos & derivados , Adamantano/sangue , Adamantano/isolamento & purificação , Sequência de Aminoácidos , Animais , Bovinos , Humanos , Dados de Sequência Molecular , Nitrilas/sangue , Nitrilas/isolamento & purificação , Fosfopeptídeos/química , Proteômica , Pirrolidinas/sangue , Pirrolidinas/isolamento & purificação , Soroalbumina Bovina/química , Soroalbumina Bovina/isolamento & purificação , Soroalbumina Bovina/metabolismo , Titânio/química , Tripsina/metabolismo , VildagliptinaRESUMO
Cross polarization-magic angle spinning (CPMAS) is the most used experiment for solid-state NMR measurements in the pharmaceutical industry, with the well-known variant RAMP-CPMAS its dominant implementation. The experimental work presented in this contribution focuses on the entangled effects of the main parameters of such an experiment. The shape of the RAMP-CP pulse has been considered as well as the contact time duration, and a particular attention also has been devoted to the radio-frequency (RF) field inhomogeneity. (13)C CPMAS NMR spectra have been recorded with a systematic variation of (13)C and (1)H constant radiofrequency field pair values and represented as a Hartmann-Hahn matching two-dimensional map. Such a map yields a rational overview of the intricate optimal conditions necessary to achieve an efficient CP magnetization transfer. The map also highlights the effects of sweeping the RF by the RAMP-CP pulse on the number of Hartmann-Hahn matches crossed and how RF field inhomogeneity helps in increasing the CP efficiency by using a larger fraction of the sample. In the light of the results, strategies for optimal RAMP-CPMAS measurements are suggested, which lead to a much higher efficiency than constant amplitude CP experiment.
Assuntos
Adamantano/isolamento & purificação , Glicina/isolamento & purificação , Ibuprofeno/isolamento & purificação , Prótons , Adamantano/química , Interpretação Estatística de Dados , Glicina/química , Ibuprofeno/química , Ressonância Magnética Nuclear Biomolecular , Ondas de Rádio , Razão Sinal-RuídoRESUMO
Platensimycin (1a) and platencin (2) are inhibitors of FabF and FabF/H bacterial fatty acid synthase. The discovery of natural congeners is an approach that can render a better understanding of the structure-function relationships of complex natural products. The isolation and structure elucidation of nine new congeners (11-20) of platensimycin and platencin are described from a fermentation broth of Streptomyces platensis. These hydroxylated congeners are likely derived by cytochrome P450 oxidation of the terpenoid units post-cyclization. Polar groups in the terpenoid portion of the molecule produce negative interactions with the hydrophobic pocket of FabF, resulting in poor activities. However, the discovery of these compounds serves an important purpose, not only to understand structure-function relationships, which cannot be easily accessed by chemical modification, but also to provide access to compounds that could be used for structural identification/confirmation of the oxidative trace metabolites produced in vivo during animal experiments.
Assuntos
Adamantano/química , Aminobenzoatos/química , Aminofenóis/química , Anilidas/química , Compostos Policíclicos/química , Streptomyces/química , Adamantano/isolamento & purificação , Adamantano/farmacologia , Aminobenzoatos/isolamento & purificação , Aminobenzoatos/farmacologia , Aminofenóis/isolamento & purificação , Aminofenóis/farmacologia , Anilidas/isolamento & purificação , Anilidas/farmacologia , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Sistema Enzimático do Citocromo P-450/metabolismo , Ácido Graxo Sintase Tipo II/antagonistas & inibidores , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Compostos Policíclicos/isolamento & purificação , Compostos Policíclicos/farmacologia , Estereoisomerismo , Relação Estrutura-AtividadeRESUMO
For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies, and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropriate exploitation of the chemical diversity of these microbial sources relies on proper understanding of their biological diversity and other related key factors that maximize the possibility of successful identification of novel molecules. Without doubt, the discovery of platensimycin has shown that microbial natural products can continue to deliver novel scaffolds if appropriate tools are put in place to reveal them in a cost-effective manner. Whereas today innovative technologies involving exploitation of uncultivated environmental diversity, together with chemical biology and in silico approaches, are seeing rapid development in natural products research, maximization of the chances of exploiting chemical diversity from microbial collections is still essential for novel drug discovery. This work provides an overview of the integrated approaches developed at the former Basic Research Center of Merck Sharp and Dohme in Spain to exploit the diversity and biosynthetic potential of actinomycetes, and includes some examples of those that were successfully applied to the discovery of novel antibiotics.
Assuntos
Actinobacteria/química , Produtos Biológicos/isolamento & purificação , Descoberta de Drogas/métodos , Adamantano/isolamento & purificação , Aminobenzoatos/isolamento & purificação , Anilidas/isolamento & purificação , Antibacterianos/isolamento & purificação , Biodiversidade , Produtos Biológicos/química , Indústria Farmacêutica , Fermentação , Ensaios de Triagem em Larga Escala , EspanhaRESUMO
Three new homoadamantane-type polyprenylated acylphloroglucinols, hyperacmosins E-G (1-3), with seven known compounds were isolated from the air-dried aerial parts of Hypericum asmosepalum. Their structures were determined by NMR, HRESIMS and experimental electronic circular dichroism (ECD) spectra. The hepatoprotective activity of these compounds were evaluated. Compounds 4 and 8 exhibited hepatoprotective activity against paracetamol-induced HepG2 cell damage.
Assuntos
Hypericum/química , Floroglucinol/análogos & derivados , Compostos Policíclicos/isolamento & purificação , Adamantano/análogos & derivados , Adamantano/isolamento & purificação , Células Hep G2 , Humanos , Compostos Policíclicos/química , Substâncias Protetoras/isolamento & purificaçãoRESUMO
Hypersubones D-H (1-5), five new polycyclic polyprenylated acylphloroglucinols (PPAPs) type metabolites with intriguing adamantane and homo-adamantane skeletons, were characterized from aerial parts of Hypericum subsessile. Compounds 1-2 were elucidated to share an adamantane core with 28,29-expoxide moiety, while 3-5 were homo-adamantane type PPAPs sharing a1,2-dioxepane ring system. Their structures were determined on the basis of comprehensive NMR and MS spectroscopic data.The anti-adipogenesis activities of these isolates were evaluated through employing 3T3-L1 cells as an in vitro system using oil red O staining, and compounds 1, 2 and 5 were able to significant inhibit the adipocyte differentiation, which implied that these compounds possessed anti-adipogenic activity.
Assuntos
Adamantano/farmacologia , Adipócitos/efeitos dos fármacos , Hypericum/química , Células 3T3-L1 , Adamantano/isolamento & purificação , Animais , Diferenciação Celular/efeitos dos fármacos , China , Camundongos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Componentes Aéreos da Planta/químicaRESUMO
Natural products continue to serve as one of the best sources for discovery of antibacterial agents as exemplified by the recent discoveries of platensimycin and platencin. Chemical modifications as well as discovery of congeners are the main sources for gaining knowledge of structure-activity relationship of natural products. Screening for congeners in the extracts of the fermentation broths of Streptomyces platensis led to the isolation of platencin A(1), a hydroxy congener of platencin. The hydroxylation of the tricyclic enone moiety negatively affected the antibacterial activity and appears to be consistent with the hydrophobic binding pocket of the FabF. Isolation, structure, enzyme-bound structure and activity of platencin A(1) and two other congeners have been described.
Assuntos
Adamantano/análogos & derivados , Aminobenzoatos/química , Antibacterianos/química , Streptomyces/química , Adamantano/química , Adamantano/isolamento & purificação , Adamantano/farmacologia , Aminobenzoatos/isolamento & purificação , Aminobenzoatos/farmacologia , Aminofenóis/química , Aminofenóis/isolamento & purificação , Aminofenóis/farmacologia , Anilidas/química , Anilidas/farmacologia , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Cristalografia por Raios X , Conformação Molecular , Compostos Policíclicos/química , Compostos Policíclicos/isolamento & purificação , Compostos Policíclicos/farmacologia , Relação Estrutura-AtividadeRESUMO
Hookeriones I-Q (1-9), nine new homo-adamantane type polycyclic polyprenylated acylphloroglucinols (PPAPs), were isolated from Hypericum hookerianum, along with twenty known analogues. These structures were determined on the basis of comprehensive NMR and MS spectroscopic data. Comprehensive analysis of the NMR data revealed the correlations between the configuration of H-18 and H-28 and the chemical shifts of related signals. The cytotoxicity and anti-allergic activities of the new isolates were evaluated, and several ones exhibited moderate cytotoxicity against ECA-109 cell lines.
Assuntos
Adamantano/farmacologia , Hypericum/química , Floroglucinol/farmacologia , Adamantano/isolamento & purificação , Animais , Linhagem Celular , Linhagem Celular Tumoral , China , Humanos , Estrutura Molecular , Floroglucinol/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Componentes Aéreos da Planta/química , RatosRESUMO
Platensimycins B(1)-B(3) are natural congeners of platensimycin with modest to significant changes in the benzoic acid portion of the molecule, leading to attenuation in the biological activities and thus confirming the significance of the free carboxylate for the potent activity.
Assuntos
Adamantano/isolamento & purificação , Adamantano/farmacologia , Aminobenzoatos/isolamento & purificação , Aminobenzoatos/farmacologia , Anilidas/isolamento & purificação , Anilidas/farmacologia , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Ácidos Graxos/biossíntese , Streptomyces/química , 3-Oxoacil-(Proteína de Transporte de Acila) Sintase/antagonistas & inibidores , 3-Oxoacil-(Proteína de Transporte de Acila) Sintase/metabolismo , Adamantano/química , Adamantano/metabolismo , Aminobenzoatos/química , Aminobenzoatos/metabolismo , Anilidas/química , Anilidas/metabolismo , Antibacterianos/química , Antibacterianos/metabolismo , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/enzimologiaRESUMO
The structure of sinaicinone, isolated from the aerial parts of the Egyptian medicinal plant Hypericum sinaicum, has been elucidated by means of spectroscopic data such as UV, IR, MS, 1D and 2D NMR spectra, and chemical degradation. It is a complex adamantanyl derivative with a unique skeleton and oxygenated side chains.
Assuntos
Adamantano/análogos & derivados , Adamantano/química , Hypericum/química , Adamantano/isolamento & purificação , Egito , Estrutura Molecular , Componentes Aéreos da Planta/química , Plantas Medicinais/químicaRESUMO
A simultaneous determination of amantadine, rimantadine, and memantine in processed products (deep-fried chicken, fried chicken, fried quail egg, and grilled chicken) with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. This new method was also applicable for chicken tissue (muscle, liver, and gizzard) and eggs. The chromatographic separation was performed on a Kinetex® XB-C18 core-shell technology column using a mobile phase of acetonitrile and 0.1% formic acid in a 10mmol/L ammonium formate solution, resulting in the complete separation of isomers (rimantadine and memantine) and any other obstructive peaks from the sample matrices. Sample preparation was performed by a modified QuEChERS method using acetonitrile and a 0.1% acetic acid extraction solution and cleaned using an Oasis® MCX cartridge. The sample matrix had no effect on the identification of the compounds. For quantification, an external solvent calibration curve was used. This new method exhibited good accuracy ranging from 79.9% to 91.5%. The relative standard deviation of repeatability (RSDr) ranged from 1.2% to 3.6% and the relative standard deviation of within-laboratory reproducibility (RSDWR) ranged from 1.3% to 6.0%. These standard deviations satisfied the criteria for Japanese validation guidelines. The limit of quantification (LOQ) was 1.0µg/kg for all samples. Analyte residues were not detected in 55 samples using the validated method.
Assuntos
Adamantano/análise , Cromatografia Líquida/métodos , Ovos/análise , Carne/análise , Espectrometria de Massas em Tandem/métodos , Adamantano/química , Adamantano/isolamento & purificação , Animais , Galinhas , Modelos Lineares , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Introduction Legal highs also known as novel psychoactive substances mimic the effects of classic drugs of abuse. Challenges to developing screening services for novel psychoactive substances include identifying which novel psychoactive substances are available to target. Using new techniques such as exact mass time of flight can help identify common novel psychoactive substances to target for screening patient samples by routine methods such as tandem mass spectrometry. We demonstrate this strategy working in our own clinical toxicology laboratory after qualitative analysis of 98 suspect materials for novel psychoactive substances by ultra-performance liquid chromatography with time of flight mass spectrometry. Results From July 2014 to July 2015 we received 98 requests to test a range of different suspect materials for novel psychoactive substances including herbs, tobacco, liquids, pills and powders. Overall, 87% of the suspect materials tested positive for novel psychoactive substances, and 15% for controlled drugs. Three common novel psychoactive substances were present in 74% of the suspect materials: methiopropamine, a methamphetamine analogue; ethylphenidate, a cocaine mimic; and the third generation synthetic cannabinoid 5F-AKB-48. For the 55 branded products we tested only 24% of the stated contents matched exactly the compounds we detected. Conclusion Testing suspect materials using ultra-performance liquid chromatography with time of flight mass spectrometry has identified three common novel psychoactive substances in use in the UK, simplifying the development of a relevant novel psychoactive substances screening service to our population. By incorporating this into our routine liquid chromatography tandem mass spectrometry drugs of abuse screen, then offers a clinically relevant novel psychoactive substances service to our users. This strategy ensures our clinical toxicology service continues to remain effective to meet the challenges of the changing drug use in the UK.
Assuntos
Adamantano/análogos & derivados , Drogas Ilícitas/química , Indazóis/isolamento & purificação , Metanfetamina/análogos & derivados , Metilfenidato/análogos & derivados , Tiofenos/isolamento & purificação , Adamantano/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Humanos , Extração Líquido-Líquido/métodos , Metanfetamina/isolamento & purificação , Metilfenidato/isolamento & purificação , Plantas Medicinais/química , Pós/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Nicotiana/químicaRESUMO
Streptomyces platensis MA7327 is a bacterium producing interesting antibiotics, which act by the novel mechanism of inhibiting fatty acid biosynthesis. The antibiotics produced by this actinomycete are platensimycin and platencin plus some minor related antibiotics. Platensimycin and platencin have activity against antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus; they also lack toxicity in animal models. Platensimycin also has activity against diabetes in a mouse model. We have been interested in studying the effects of primary metabolites on production of these antibiotics in our chemically defined production medium. In the present work, we tested 32 primary metabolites for their effect. They included 20 amino acids, 7 vitamins and 5 nucleic acid derivatives. Of these, only l-aspartic acid showed stimulation of antibiotic production. We conclude that the stimulatory effect of aspartic acid is due to its role as a precursor involved in the biosynthesis of aspartate-4-semialdehyde, which is the starting point for the biosynthesis of the 3-amino-2,4-dihydroxy benzoic acid portion of the platensimycin molecule.
Assuntos
Antibacterianos/isolamento & purificação , Ácido Aspártico/administração & dosagem , Streptomyces/metabolismo , Adamantano/isolamento & purificação , Aminoácidos/administração & dosagem , Aminoácidos/metabolismo , Aminobenzoatos/isolamento & purificação , Aminofenóis/isolamento & purificação , Anilidas/isolamento & purificação , Antibacterianos/biossíntese , Ácido Aspártico/química , Ácidos Nucleicos/administração & dosagem , Ácidos Nucleicos/metabolismo , Compostos Policíclicos/isolamento & purificação , Vitaminas/administração & dosagem , Vitaminas/metabolismoRESUMO
Vildagliptin is a member of a new class of oral anti-diabetic drug. One unknown impurity was identified in the range of 0.01-0.06% in different laboratory batches of vildagliptin along with known impurities by HPLC analysis. The structure of unknown impurity was proposed as (2S)-1-[2-[(3-hydroxyadamantan-1-yl)imino]acetyl]pyrrolidine-2-carbonitrile (Impurity-E) using LC/ESI-MS(n) study. The unknown impurity was found to be unstable in diluent (H2O:CH3CN) and degrading into another stable impurity. The degraded stable impurity was isolated from enriched reaction crude sample by semi preparative liquid chromatography. The structure of stable impurity was established using FT-IR, NMR ((1)H, (13)C and DEPT), 2D NMR (HSQC, HMBC and COSY) and mass spectral data as (8aS)-3-hydroxy-octahydropyrrolo[1,2-a]piperazine-1,4-dione (Impurity-F). Impurity identification, abnormal behaviour of impurity-E, isolation of impurity-F, fragmentation mechanism and structural elucidation were also discussed.
Assuntos
Adamantano/análogos & derivados , Inibidores da Dipeptidil Peptidase IV/análise , Contaminação de Medicamentos/prevenção & controle , Nitrilas/análise , Pirrolidinas/análise , Pirrolidinas/isolamento & purificação , Tecnologia Farmacêutica/métodos , Adamantano/análise , Adamantano/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray , Tecnologia Farmacêutica/normas , VildagliptinaRESUMO
Platensimycin and platencin are inhibitors of FabF and FabF/H bacterial fatty acid synthesis enzymes, respectively. Discovery of natural congeners provides one of the ways to understand the relationship of chemical structure and biological function. Efforts to discover the natural analogs of platensimycin by chemical screening led to the isolation of platensimycin B(4), a glucoside congener of platensimycin. This analog showed significantly attenuated activity and critically defined the limited binding space around the aromatic ring and established the importance of the free phenolic and carboxyl group for the activity.
Assuntos
Adamantano/química , Adamantano/farmacologia , Aminobenzoatos/química , Aminobenzoatos/farmacologia , Anilidas/química , Anilidas/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Streptomyces/química , Adamantano/isolamento & purificação , Aminobenzoatos/isolamento & purificação , Anilidas/isolamento & purificação , Proteínas de Bactérias/antagonistas & inibidores , Inibidores Enzimáticos/isolamento & purificação , Ácidos Graxos/biossíntese , Concentração Inibidora 50 , Testes de Sensibilidade Microbiana , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/enzimologia , Relação Estrutura-AtividadeRESUMO
Fatty acids are essential for survival of bacteria and are synthesized by a series of enzymes including the elongation enzymes, beta-ketoacyl acyl carrier protein synthase I/II (FabF/B). Inhibition of fatty acid synthesis is one of the new targets for the discovery and development of antibacterial agents. Platensimycin (1a) is a novel broad spectrum Gram-positive antibiotic produced by Streptomyces platensis. It was discovered by target-based whole-cell screening strategy using antisense differential sensitivity assay. It inhibits bacterial growth by selectively inhibiting condensing enzyme FabF of the fatty acid synthesis pathway and was isolated by a two-step process, a capture step followed by reversed-phase HPLC. The structure was elucidated by 2D NMR methods and confirmed by X-ray crystallographic analysis of a bromo derivative. It was determined that potential reactivity of the enone moiety does not play a key role in the biological activity of platensimycin. However, cyclohexenone ring conformation renders for the stronger binding interaction with the enzyme. The isolation, structure elucidation, derivatization, and biological activity of 6,7-dihydroplatensimycin are described.
Assuntos
Adamantano/química , Aminobenzoatos/química , Anilidas/química , Antibacterianos/química , Streptomyces/química , Adamantano/isolamento & purificação , Adamantano/farmacologia , Aminobenzoatos/isolamento & purificação , Aminobenzoatos/farmacologia , Anilidas/isolamento & purificação , Anilidas/farmacologia , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Cristalografia por Raios X , Modelos Moleculares , Conformação Molecular , Ressonância Magnética Nuclear Biomolecular , EstereoisomerismoRESUMO
Among the different experimental strategies available in capillary electrophoresis (CE) to determine binding parameters, affinity capillary electrophoresis (ACE) has been the most widely embraced due to its easiness of implementation and of data handling. Ligand-substrate binding constants are thus directly derived from the substrate migration time shifts resulting from the variation of ligand concentration introduced in a background electrolyte. Classically, the substrate migration time is measured on top of the electrophoretic peak, assuming symmetrical peak shape. Depending on both substrate and ligand concentrations that may be required to meet detection sensitivity or complexation conditions, zonal migrations in ACE may, however, produce triangular peak shape, most often due to pronounced electromigration dispersion (EMD), and this may result in positively or negatively erroneous migration time assessments. In this work, EMD distorted triangular peak shapes obtained in the course of host-guest complexation studies were fitted with the Haarhoff-Van der Linde function, allowing better estimation of migration time. The model systems studied were those of beta-cyclodextrin and naproxen, 2-naphthalenesulfonate, or 1-adamantanecarboxylate. The impact of this correction on binding isotherms and binding constant evaluation was exemplified. Furthermore, in situations where the substrate concentration injected by far overtakes that of the ligand in the electrolyte, the interest in this peak shape correction was discussed in connection with the question of whether the free ligand concentration can be still considered equal to the ligand concentration introduced, a question that still remains under debate nowadays.
Assuntos
Eletroforese Capilar/métodos , Adamantano/análogos & derivados , Adamantano/isolamento & purificação , Eletrólitos/química , Modelos Químicos , Naftalenossulfonatos/isolamento & purificação , Naproxeno/isolamento & purificação , beta-Ciclodextrinas/químicaRESUMO
Intact cells of (+/-)camphor-grown Pseudomonas putida, ATCC17453(CAM), have been shown to oxidize readily the monoketone derivative of cage hydrocarbon adamantane, forming oxygenated products indicative of both biological Baeyer-Villiger and hydroxylation reactions. Formed products were identified as 4-oxahomoadamantan-5-one, 5-hydroxyadamantan-2-one and 1-hydroxy-4-oxahomoadamantan-5-one. Minor products formed as a result of secondary reactions were tentatively identified as syn- and anti-1,4-dihydroxyadamantanes and bicyclo[3.3.1]nonan-3-ol. Adamantanone initial concentrations determined whether 1-hydroxy-4-oxahomoadamantan-5-one was the sole product (below 120 mg/l) or 4-oxahomoadamantan-5-one was the principle (up to 92%) product (240-600 mg/l). Formation of 1-hydroxy-4-oxahomoadamantan-5-one appears to occur by two routes determined by the sequence of lactonization and hydroxylation.