Stage-associated expression of ceramide structures in glycosphingolipids from the human trematode parasite Schistosoma mansoni.
Biochim Biophys Acta
; 1524(2-3): 155-61, 2000 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-11113562
ABSTRACT
Glycosphingolipids of Schistosoma mansoni adults, cercariae and eggs comprise ceramide monohexosides (CMH) with glucose or galactose and ceramide dihexosides (CDH) with the schistosome-specific structure GalNAc(beta1-4)Glc(1-1)ceramide. Ceramide analysis revealed C18- and C20-phytosphingosines in egg CMH, C18-sphinganine as well as C18-, C19- and C20-phytosphingosines in cercarial CMH, and C18- and C20-phytosphingosines as well as C18-sphingosine and C18-sphinganine in adult CMH. For all three life cycle stages, the predominant fatty acid was C16h0. As a characteristic feature, a range of saturated, unsaturated and hydroxylated long-chain fatty acids with 24-28 carbon atoms were additionally found in minor cercarial CMH species. The corresponding ceramides represented major constituents in cercarial CDH, while adult and egg CDH were dominated by ceramides with short fatty acid chains. The resultant ceramide patterns could be correlated with the differential expression of carbohydrate antigens on schistosomal glycolipids at various stages. A possible impact of ceramide structure on the biosynthesis of the carbohydrate moieties is discussed.
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Base de dados:
MEDLINE
Assunto principal:
Schistosoma mansoni
/
Glicoesfingolipídeos
/
Ceramidas
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Alemanha