Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors.
J Cancer Res Clin Oncol
; 127(12): 733-8, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11768613
ABSTRACT
PURPOSE:
The constitutive activation of Ras proteins by point mutation is the most frequently observed oncogene activation in human malignancies. The goal of this study was to investigate whether the constitutive activation of RhoA, Rac1, and Cdc42 proteins by point mutations, which can lead to experimental transformation of cultured cells, actually occurred in a panel of invasive colorectal and breast tumors.METHODS:
We performed denaturing gradient gel electrophoresis and sequencing of transcripts amplified by reverse transcription and PCR for RhoA; we used direct sequencing of PCR-amplified genomic DNA to search for mutations in coding exons of the Rac1 and Cdc42 genes.RESULTS:
Although mutations of the Kras4B and the p53 genes were detected using these methods, no mutation was found in the coding sequences of RhoA, Rac1, and Cdc42 genes, in primary as well as in associated metastasis.CONCLUSIONS:
Point mutations in the coding sequences of genes encoding RhoA, Rac1, and Cdc42 GTPases do not occur at high frequency in invasive breast and colorectal tumors.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Neoplasias Colorretais
/
Adenocarcinoma
/
Proteína cdc42 de Ligação ao GTP
/
Proteínas rac1 de Ligação ao GTP
/
Proteína rhoA de Ligação ao GTP
/
Mutação
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
França