pCLCA1 becomes a cAMP-dependent chloride conductance mediator in Caco-2 cells.
Biochem Biophys Res Commun
; 298(4): 531-6, 2002 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-12408984
ABSTRACT
Members of the CLCA protein family are expressed in airway and intestinal epithelium, where they may participate in secretory activity as mediators of chloride conductance. A calcium-dependent chloride conductance has been observed upon expression of CLCA proteins in non-epithelial cell lines. The pCLCA1 gene, cloned in our laboratory, codes for a product containing a unique A-kinase consensus acceptor site not found in other CLCA proteins. Calcium-dependent, but not cAMP-dependent, chloride conductance increased when pCLCA1 was expressed in NIH/3T3 fibroblasts. We transfected the Caco-2 human colon carcinoma cell line with pCLCA1 to investigate the regulation of CLCA-associated chloride conductance in this differentiated epithelial cell line. Expression of pCLCA1 in the Caco-2 cell line enhanced cAMP-responsive 36Cl efflux, short circuit current, and whole cell chloride current in these cells. This cAMP-dependent chloride conductance was localized to the apical membrane of polarized Caco-2 cells.
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Base de dados:
MEDLINE
Assunto principal:
Cloretos
/
Canais de Cloreto
/
AMP Cíclico
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article