Early prognosis of the development of renal chronic allograft rejection by gene expression profiling of human protocol biopsies.
Transplantation
; 75(8): 1323-30, 2003 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-12717224
ABSTRACT
BACKGROUND:
Chronic allograft rejection (CR) is the major cause of failure of long-term graft survival and is so far irreversible. Early prognosis of CR by molecular markers before overt histologic manifestation would be a valuable aid for the optimization of treatment regimens and the design of clinical CR trials. Oligonucleotide microarray-based approaches have proven to be useful for the diagnosis and prognosis of a variety of diseases and were chosen for the unbiased identification of prognostic biomarkers.METHODS:
Renal allograft biopsies were taken at month 6 posttransplantation (PT) from two groups who were, at that time, healthy recipients one group developed CR at month-12 PT, the other group remained healthy. Gene expression profiles from the two groups at month-6 PT biopsies were analyzed to identify differentially expressed genes with prognostic value for CR development at month 12.RESULTS:
A set of 10 genes was identified that showed differential expression profiles between the two patient groups and had a complete separation of the 15% to 85% quantile range for each individual gene. This set of genes was sufficient to allow the correct prediction of the occurrence or nonoccurrence of CR in 15 of 17 (88%) patients using cross-validation (occurrence for a patient was predicted on the basis of the other patients' data only). In addition, a correct prediction could be made that a recipient with a normal biopsy 12 months PT developed CR within the following 6 months.CONCLUSIONS:
Identified expression patterns seem to be highly prognostic of the development of renal CR.
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Base de dados:
MEDLINE
Assunto principal:
Transplante de Rim
/
Perfilação da Expressão Gênica
/
Rejeição de Enxerto
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Suíça