Identification of cytoskeletal regulatory proteins required for efficient phagocytosis in Drosophila.
Microbes Infect
; 5(10): 815-24, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-12919849
ABSTRACT
Phagocytosis is a complex and apparently evolutionarily conserved process that plays a central role in the immune response to infection. By ultrastructural and functional criteria, Drosophila hemocyte (macrophage) phagocytosis resembles mammalian phagocytosis. Using a non-saturated forward genetic screen for larval hemocyte phagocytosis mutants, D-SCAR and profilin were identified as important regulators of phagocytosis in Drosophila. In both hemocytes ex vivo and the macrophage-like S2 cell line, lack of D-SCAR significantly decreased phagocytosis of Escherichia coli and Staphylococcus aureus. In contrast, profilin mutant hemocytes exhibited increased phagocytic activity. Analysis of double mutants suggests that D-SCAR and profilin interact during phagocytosis. Finally, RNA interference studies in S2 cells indicated that the D-SCAR homolog D-WASp also participates in phagocytosis. This study demonstrates that Drosophila provides a viable model system in which to dissect the complex interactions that regulate phagocytosis.
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Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Proteínas Contráteis
/
Proteínas de Drosophila
/
Drosophila
/
Hemócitos
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos