Sponge-cell culture? A molecular identification method for sponge cells.
Mar Biotechnol (NY)
; 5(5): 443-9, 2003.
Article
em En
| MEDLINE
| ID: mdl-14730427
ABSTRACT
Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.
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Base de dados:
MEDLINE
Assunto principal:
Poríferos
/
Técnicas de Cultura de Células
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Holanda