Detection of somatic TP53 splice site mutations in diffuse astrocytomas.
Cancer Lett
; 224(2): 321-7, 2005 Jun 28.
Article
em En
| MEDLINE
| ID: mdl-15914282
ABSTRACT
Alteration in TP53 is the most common genetic event reported for many tumors, including astrocytomas. The majority of studies, on analyzing TP53 mutations, have not included all splice junctions. Consequently, splice site mutations are thought to be relatively infrequent. TP53 were examined for mutations by polymerase chain reaction, single strand conformation polymorphism and direct sequencing in cases of diffuse astrocytomas. We found TP53 mutations in 17.8% (8 out of 45) of the tumors tested 3 splicing, 3 missense and 2 silent mutations. We have shown that splice site mutations of TP53 are more frequent than previously reported. These findings emphasize the importance of thorough screening of TP53 mutations in gliomas.
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Base de dados:
MEDLINE
Assunto principal:
Astrocitoma
/
Neoplasias Encefálicas
/
Genes p53
/
Sítios de Splice de RNA
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil